FastQCFastQC Report
Thu 26 May 2016
SRR937542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777350
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191560.6897222172214521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154700.5570057788899491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114380.41183142203899403No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60190.21671737447566927No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.12753163987254038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.10852071218967721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150000.031.1671981
GTATCAA214850.029.7427331
TACATGG139850.024.0497192
ATCAACG264950.023.9362743
TATCAAC267500.023.8856852
TCAACGC266900.023.7435954
GTACATG141700.023.7051941
CAACGCA269250.023.5010745
AACGCAG274500.023.117086
ACATGGG140250.023.1004643
AGTACTT157900.023.08853312-13
GAGTACT155950.022.88989312-13
AGAGTAC237250.021.9934810-11
GTACTTT163750.021.60945514-15
TAAGGTG10750.021.211535
GTAAGGT10050.020.7982034
TACTTTT174850.020.79449714-15
ACGCAGA306400.020.6497887
CGCAGAG307650.020.5041268
CATGGGA80450.019.8404944
Produced by FastQC (version 0.11.5)