FastQCFastQC Report
Thu 26 May 2016
SRR937541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624599
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177690.6770177082289522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131570.5012956264937996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90760.34580520681445054No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46530.17728422513305842No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36740.13998328887574824No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30930.11784657389566938No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.10691157011032924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127050.032.0313531
GTATCAA187100.031.5590551
ATCAACG226350.025.8861773
TATCAAC228350.025.7272912
TCAACGC228250.025.6488934
GTACATG133650.025.5050621
CAACGCA229900.025.4225045
AACGCAG233550.025.0577456
TACATGG132500.024.8954622
GAGTACT129900.024.22310612-13
ACATGGG136150.023.4899863
AGTACTT131500.023.4588412-13
GTACTTT137200.022.77892314-15
CATGGGG61750.022.3936444
CGCAGAG262200.022.2472748
ACGCAGA263150.022.2211137
AGAGTAC204250.021.97532510-11
GTGTAAG7200.021.1297151
TAAGGTG10000.020.907635
GCAGAGT271800.020.8315419
Produced by FastQC (version 0.11.5)