FastQCFastQC Report
Thu 26 May 2016
SRR937541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624599
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180910.6892862490612852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147200.5608475809066452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105490.40192806596360053No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60020.22868255303000576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33980.12946739673374866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.10603524576516261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA204000.029.0704481
GGTATCA144850.028.7705671
TAAGGTG10150.023.8669915
ATCAACG250600.023.5604523
TATCAAC252300.023.4770072
TACATGG128950.023.4276812
GTACATG133450.023.3945181
TCAACGC253650.023.2771474
GAGTACT142150.023.27515612-13
CAACGCA256600.022.9910345
AACGCAG262250.022.4944216
AGTACTT146200.022.12677412-13
GTACTTT150100.021.83439814-15
ACCGTCG2657.930794E-1021.510668
ACATGGG134250.021.2644273
AGAGTAC222950.021.26382610-11
GTAAGGT9850.020.7360384
ACGCAGA292250.020.05767
CGCAGAG293250.019.9082098
TACTTTT161550.019.8899514-15
Produced by FastQC (version 0.11.5)