##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937541_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2624599 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06061764101869 31.0 31.0 34.0 30.0 34.0 2 32.28432724389516 34.0 31.0 34.0 31.0 34.0 3 32.33627270299196 34.0 31.0 34.0 30.0 34.0 4 36.001173512601355 37.0 35.0 37.0 35.0 37.0 5 35.91044765314625 37.0 35.0 37.0 35.0 37.0 6 35.96016381931106 37.0 35.0 37.0 35.0 37.0 7 35.906357504517835 37.0 35.0 37.0 35.0 37.0 8 35.867181996183035 37.0 35.0 37.0 35.0 37.0 9 37.51937610278751 39.0 37.0 39.0 35.0 39.0 10-11 37.42908859601029 39.0 37.0 39.0 34.5 39.0 12-13 37.3066184586674 39.0 37.0 39.0 34.0 39.0 14-15 38.43868911022217 40.0 38.0 41.0 34.0 41.0 16-17 38.40266074931828 40.0 38.0 41.0 34.0 41.0 18-19 38.385251042159204 40.0 38.0 41.0 34.0 41.0 20-21 38.26901861960627 40.0 38.0 41.0 34.0 41.0 22-23 38.21052930371459 40.0 38.0 41.0 33.5 41.0 24-25 38.21052015946055 40.0 38.0 41.0 33.5 41.0 26-27 37.99615788926232 40.0 37.0 41.0 33.0 41.0 28-29 37.94827457451595 40.0 37.0 41.0 33.0 41.0 30-31 37.94369139819074 40.0 37.0 41.0 33.0 41.0 32-33 37.77865247224433 40.0 37.0 41.0 33.0 41.0 34-35 37.59188203607485 40.0 36.0 41.0 33.0 41.0 36-37 37.38434480848312 40.0 36.0 41.0 32.5 41.0 38-39 37.211374385191796 39.0 36.0 41.0 32.0 41.0 40-41 37.00157776483188 39.0 36.0 41.0 31.0 41.0 42-43 36.723910204949405 39.0 35.0 41.0 31.0 41.0 44-45 36.344629598654876 39.0 35.0 40.0 30.0 41.0 46-47 35.90949188809414 39.0 35.0 40.0 29.5 41.0 48-49 35.47675663977621 39.0 35.0 40.0 27.5 41.0 50-51 35.16455408997717 38.0 35.0 40.0 26.5 41.0 52-53 34.85584902684182 38.0 34.0 40.0 26.0 41.0 54-55 34.58161742041356 38.0 34.0 40.0 25.5 41.0 56-57 34.24954516861433 38.0 34.0 40.0 24.5 41.0 58-59 33.952309286104274 37.0 33.0 40.0 24.0 41.0 60-61 33.53501773032757 37.0 33.0 39.5 22.5 41.0 62-63 33.08140900762364 36.0 32.0 39.0 22.0 40.5 64-65 32.63528066573217 36.0 32.0 39.0 20.5 40.0 66-67 32.16051347272479 35.0 31.0 38.5 19.5 40.0 68-69 31.63477030205376 35.0 31.0 38.0 18.0 40.0 70-71 31.16349126095072 35.0 30.0 37.0 14.5 39.0 72-73 30.654628383231113 34.0 30.0 36.5 11.0 39.0 74-75 30.150359921648985 34.0 29.0 36.0 7.5 39.0 76-77 28.98824429941488 32.5 28.0 35.0 7.0 37.0 78-79 29.60885929622011 34.0 29.0 35.0 7.0 37.0 80-81 29.44096888705665 34.0 29.0 35.0 2.0 37.0 82-83 29.030420456610706 34.0 29.0 35.0 2.0 36.0 84-85 28.597166843392074 33.5 29.0 35.0 2.0 36.0 86-87 28.20714097658347 33.0 27.5 35.0 2.0 35.5 88-89 27.742502568963868 33.0 27.0 35.0 2.0 35.0 90-91 27.158270463411743 33.0 25.5 35.0 2.0 35.0 92-93 26.601680675790853 32.0 25.0 34.0 2.0 35.0 94-95 25.99314180947261 32.0 24.0 34.0 2.0 35.0 96-97 25.33314308204796 32.0 21.5 34.0 2.0 35.0 98-99 24.333815565730234 31.0 10.0 34.0 2.0 35.0 100-101 21.829881821946895 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15842972530191446 1101 2 0.21420731848586172 1101 3 0.1851751575274534 1101 4 0.13753188189975418 1101 5 0.08893063550534919 1101 6 0.11111757329170047 1101 7 0.07454621284979623 1101 8 0.11922338286436229 1101 9 0.12666105954640727 1101 10-11 0.1571220864430174 1101 12-13 0.173497797224357 1101 14-15 0.2268874144779076 1101 16-17 0.21583819179663521 1101 18-19 0.1934536277548986 1101 20-21 0.1609253340120702 1101 22-23 0.13123490248697323 1101 24-25 0.1468922687846117 1101 26-27 0.06338307595949999 1101 28-29 0.19592781848309215 1101 30-31 0.32427783062433235 1101 32-33 0.3288111423788749 1101 34-35 0.3084996915614724 1101 36-37 0.28677504097413475 1101 38-39 0.382480326654175 1101 40-41 0.2689983753697476 1101 42-43 0.38664093982988135 1101 44-45 0.4982163239147894 1101 46-47 0.46595375493394187 1101 48-49 0.3597636605108647 1101 50-51 0.41295574831248416 1101 52-53 0.3530085826307854 1101 54-55 0.3974917486566838 1101 56-57 0.34036725620862285 1101 58-59 0.45372380080207364 1101 60-61 0.4662637112827923 1101 62-63 0.39987798341400804 1101 64-65 0.4265255380714521 1101 66-67 0.2666532550902758 1101 68-69 0.34088503901019607 1101 70-71 0.38048447031974675 1101 72-73 0.20029644845195804 1101 74-75 0.2015603707563649 1101 76-77 0.20945871522991766 1101 78-79 0.38780982570826694 1101 80-81 0.5198055934047368 1101 82-83 0.4967086650951984 1101 84-85 0.5883000168075476 1101 86-87 0.4826082322676619 1101 88-89 0.632220433934652 1101 90-91 0.5502452739372536 1101 92-93 0.7447600140789739 1101 94-95 0.7468495136508047 1101 96-97 0.8549302683315929 1101 98-99 0.9998243836381029 1101 100-101 1.1986074344511835 1102 1 0.12095690448342111 1102 2 0.14159143542884323 1102 3 0.1450256780378254 1102 4 0.08790994691828047 1102 5 0.05579126178419358 1102 6 0.05250227347548275 1102 7 0.04365768394652747 1102 8 0.13352439920952008 1102 9 0.1623621262041013 1102 10-11 0.11457915655529405 1102 12-13 0.05299908628266081 1102 14-15 0.12680854077357395 1102 16-17 0.19233651177471245 1102 18-19 0.1534751948460169 1102 20-21 0.15940333990536715 1102 22-23 0.2321740163134578 1102 24-25 0.16480228786812035 1102 26-27 0.1450812520501472 1102 28-29 0.11151252539657719 1102 30-31 0.1096572335716175 1102 32-33 0.16440233063586618 1102 34-35 0.1759047426831657 1102 36-37 0.235358719204676 1102 38-39 0.33046133607736294 1102 40-41 0.38591492226375124 1102 42-43 0.3320594551615841 1102 44-45 0.32882633130144256 1102 46-47 0.16916169950683724 1102 48-49 0.30818730804114836 1102 50-51 0.2390650675070347 1102 52-53 0.4404926997340013 1102 54-55 0.5209164777461339 1102 56-57 0.42920953123229566 1102 58-59 0.50826262396955 1102 60-61 0.4596589899164698 1102 62-63 0.4084940042696701 1102 64-65 0.4929807398903918 1102 66-67 0.355066711929652 1102 68-69 0.341759699921365 1102 70-71 0.20797117515343544 1102 72-73 0.35934573390186486 1102 74-75 0.41369458343736554 1102 76-77 0.2879991391049792 1102 78-79 0.32745802254883216 1102 80-81 0.38447670327672867 1102 82-83 0.4855629975007929 1102 84-85 0.5328404663195592 1102 86-87 0.48592982066950086 1102 88-89 0.6925682313193597 1102 90-91 0.7132104044694323 1102 92-93 0.8320957172286363 1102 94-95 0.9723794375891579 1102 96-97 0.9103611775302909 1102 98-99 0.8963460092135342 1102 100-101 1.3667277967654954 1103 1 0.1093543179386458 1103 2 0.09710761751635744 1103 3 0.1321955760435216 1103 4 0.062321972938448766 1103 5 0.08045850050200443 1103 6 0.1311939077800801 1103 7 0.10313771471729893 1103 8 0.05286666801330142 1103 9 0.13364803349787024 1103 10-11 0.17833368425194607 1103 12-13 0.1250813787605196 1103 14-15 0.14703925842015764 1103 16-17 0.17617272845369314 1103 18-19 0.1661701122610708 1103 20-21 0.13455034573468794 1103 22-23 0.17480552920059722 1103 24-25 0.019155112910070216 1103 26-27 0.06963394897263697 1103 28-29 -0.020089563826822143 1103 30-31 0.007043997244899458 1103 32-33 0.1258605657054659 1103 34-35 0.21489878390479333 1103 36-37 0.09541075836044399 1103 38-39 0.160514979686603 1103 40-41 0.2556681324534722 1103 42-43 0.4323518584197501 1103 44-45 0.3444875141448023 1103 46-47 0.44348056657717194 1103 48-49 0.42974429495251343 1103 50-51 0.5490416302506418 1103 52-53 0.45570871087716114 1103 54-55 0.3466115040982487 1103 56-57 0.39863517043827557 1103 58-59 0.3292256473361519 1103 60-61 0.40276172467090277 1103 62-63 0.19320733204826723 1103 64-65 0.3800425593051173 1103 66-67 0.435666224949415 1103 68-69 0.528387734716496 1103 70-71 0.42452103379180883 1103 72-73 0.3926903424842898 1103 74-75 0.543585600984521 1103 76-77 0.48004837155259494 1103 78-79 0.6340713015031021 1103 80-81 0.5856427541463916 1103 82-83 0.6737126482114704 1103 84-85 0.6015189196343051 1103 86-87 0.6202115248082833 1103 88-89 0.7955126698652393 1103 90-91 0.8135811555786354 1103 92-93 0.9190894608468376 1103 94-95 0.9331632792230842 1103 96-97 1.1819877006263297 1103 98-99 1.2474023533567653 1103 100-101 1.7484764181788108 1104 1 0.2085487156724568 1104 2 0.2377762543679367 1104 3 0.1984182366445424 1104 4 0.11877848267958768 1104 5 0.1541385294888471 1104 6 0.1292682481290086 1104 7 0.09602726544014217 1104 8 0.14517818267426463 1104 9 0.1112784148592425 1104 10-11 0.22395464111014718 1104 12-13 0.16250244297665972 1104 14-15 0.298548073079715 1104 16-17 0.2949481139461625 1104 18-19 0.3422414562553371 1104 20-21 0.22640657879589554 1104 22-23 0.2634699672170058 1104 24-25 0.20121513807165314 1104 26-27 0.24674634867517398 1104 28-29 0.2951046974468241 1104 30-31 0.29636339427004543 1104 32-33 0.3781788218163129 1104 34-35 0.32106408747841186 1104 36-37 0.329944884687734 1104 38-39 0.40979784278773224 1104 40-41 0.49738690578012523 1104 42-43 0.5616898764871934 1104 44-45 0.49302553555412487 1104 46-47 0.491413737305443 1104 48-49 0.527858151058858 1104 50-51 0.47855115844164686 1104 52-53 0.6088386363601686 1104 54-55 0.7736955823511877 1104 56-57 0.7310510612033099 1104 58-59 0.6380964425457663 1104 60-61 0.6802751302399273 1104 62-63 0.5954642159010817 1104 64-65 0.7834866564436567 1104 66-67 0.9608001563137307 1104 68-69 0.9112678734682262 1104 70-71 0.8289411890361187 1104 72-73 0.7888221347461837 1104 74-75 0.9855570643018545 1104 76-77 0.6854649146807148 1104 78-79 0.7826490574835034 1104 80-81 0.7828897898926854 1104 82-83 0.8769453315720952 1104 84-85 0.8670933547196036 1104 86-87 0.9425213575879035 1104 88-89 0.8400896866533216 1104 90-91 0.9433613761567265 1104 92-93 1.2276322344909723 1104 94-95 1.2728608409526565 1104 96-97 1.449545681521311 1104 98-99 1.8208619275831843 1104 100-101 2.155048257636821 1105 1 0.10226804168556214 1105 2 0.10107404642930362 1105 3 0.10231331036947466 1105 4 0.010436376378677892 1105 5 0.013011504419871756 1105 6 -0.010394229880077432 1105 7 0.07929310937829115 1105 8 0.04431293648851664 1105 9 0.13382760834152663 1105 10-11 0.05757607670280862 1105 12-13 0.0858055783069176 1105 14-15 -0.01645068710847397 1105 16-17 0.10566936853248876 1105 18-19 0.19808840257096705 1105 20-21 0.14019020985267616 1105 22-23 0.10806595683355624 1105 24-25 0.04717941125804259 1105 26-27 0.04489182350658183 1105 28-29 0.003295734588128596 1105 30-31 0.0566243080722586 1105 32-33 0.008342235413614674 1105 34-35 0.17273046268778813 1105 36-37 0.11854298270698393 1105 38-39 0.04600779072389116 1105 40-41 0.06749656047653474 1105 42-43 0.25494567185899086 1105 44-45 0.21012071325394288 1105 46-47 0.2048756779732699 1105 48-49 0.253036932748131 1105 50-51 0.20319019358107226 1105 52-53 0.31250379261052075 1105 54-55 0.27742900567496775 1105 56-57 0.28804276365178083 1105 58-59 0.2950127446155406 1105 60-61 0.22569394816763122 1105 62-63 0.17975985723477805 1105 64-65 0.26500989894072546 1105 66-67 0.29566279734619627 1105 68-69 0.30675024078410473 1105 70-71 0.18618945304150358 1105 72-73 0.16180666631306906 1105 74-75 0.33816578939748254 1105 76-77 0.2999021257999175 1105 78-79 0.23083953098012344 1105 80-81 0.2941754324176742 1105 82-83 0.21230095522840742 1105 84-85 0.24413205026828066 1105 86-87 0.5208589857009294 1105 88-89 0.4620231654331306 1105 90-91 0.43211026461020907 1105 92-93 0.47912036997711027 1105 94-95 0.6699551700885245 1105 96-97 0.6993956859705932 1105 98-99 0.7494351405869821 1105 100-101 0.8599288114208079 1106 1 -0.2920650329709922 1106 2 -0.22913079562428607 1106 3 -0.2699819751383572 1106 4 -0.19515061047855653 1106 5 -0.1993896735621874 1106 6 -0.15735767166726333 1106 7 -0.1405939562497096 1106 8 -0.10197690487585476 1106 9 -0.32260350506780355 1106 10-11 -0.5063465979795367 1106 12-13 -0.44234500457893233 1106 14-15 -0.3713703944965516 1106 16-17 -0.24312345624585419 1106 18-19 -0.3108289653026972 1106 20-21 -0.266057608593286 1106 22-23 -0.3855555282148515 1106 24-25 -0.329600197396239 1106 26-27 -0.29748142550675283 1106 28-29 -0.30381452594432545 1106 30-31 -0.38498969032693964 1106 32-33 -0.3793872196465671 1106 34-35 -0.6002673371634017 1106 36-37 -0.425705914108903 1106 38-39 -0.43721141004863995 1106 40-41 -0.5731551933088639 1106 42-43 -0.8844333871007848 1106 44-45 -0.831919213341763 1106 46-47 -0.6577395366383385 1106 48-49 -0.6379297263790775 1106 50-51 -0.6873850868192264 1106 52-53 -0.6470035182450928 1106 54-55 -0.7227125811484072 1106 56-57 -0.8559197482698195 1106 58-59 -0.6929509157395657 1106 60-61 -0.8187884304605362 1106 62-63 -0.9254959522674184 1106 64-65 -1.2557035996461963 1106 66-67 -1.2485409769993119 1106 68-69 -1.181307408851783 1106 70-71 -1.1396763335656424 1106 72-73 -1.1024287432631468 1106 74-75 -1.0602476639273526 1106 76-77 -0.9954299641586779 1106 78-79 -0.9676679243181638 1106 80-81 -1.15511573228531 1106 82-83 -1.1300182229765383 1106 84-85 -0.904216987715575 1106 86-87 -0.7299471897438643 1106 88-89 -0.9337352969657005 1106 90-91 -1.1097316562083002 1106 92-93 -0.8494717404065426 1106 94-95 -0.667894789256831 1106 96-97 -0.7539459661439167 1106 98-99 -0.7118108133045808 1106 100-101 -0.0965597363565891 1107 1 0.1700860187058808 1107 2 0.20096174803131106 1107 3 0.12360362279220993 1107 4 0.10627852712917019 1107 5 0.11288941874202152 1107 6 0.16407509742315796 1107 7 0.057903222021373324 1107 8 0.09971723838099678 1107 9 0.15959772896762559 1107 10-11 0.15558898229512863 1107 12-13 0.1371128397451642 1107 14-15 0.24701426953600247 1107 16-17 0.1611157982817275 1107 18-19 0.24885800191803042 1107 20-21 0.19739590315592181 1107 22-23 0.19891180760136962 1107 24-25 0.2522598392940907 1107 26-27 0.254443761834807 1107 28-29 0.29786062404080127 1107 30-31 0.2743169228036493 1107 32-33 0.2697107992664627 1107 34-35 0.15901454742180476 1107 36-37 0.24778688555078787 1107 38-39 0.2625151871414957 1107 40-41 0.22961742572314137 1107 42-43 0.24516605161395688 1107 44-45 0.19804778440193616 1107 46-47 0.02950049536602961 1107 48-49 0.06563339596561946 1107 50-51 0.02921118772189857 1107 52-53 0.1058002718706419 1107 54-55 0.21640414555859877 1107 56-57 0.2409062664171131 1107 58-59 0.1488694009658431 1107 60-61 0.21694933276730666 1107 62-63 0.2160007715539507 1107 64-65 0.19351222384523226 1107 66-67 0.2526422768246164 1107 68-69 0.3638078482877951 1107 70-71 0.2621541703231607 1107 72-73 0.2961798484854157 1107 74-75 0.43189558983484844 1107 76-77 0.3184865744930825 1107 78-79 0.4091017483777364 1107 80-81 0.634745962747818 1107 82-83 0.5363154506154046 1107 84-85 0.650108205901347 1107 86-87 0.836348973100872 1107 88-89 0.9283691781165935 1107 90-91 0.9226441564709766 1107 92-93 1.0230217456856785 1107 94-95 1.072463649915452 1107 96-97 0.9432767211584192 1107 98-99 1.0212710938616567 1107 100-101 1.5509297054675457 1108 1 0.12266063521251169 1108 2 0.10469268353038785 1108 3 0.04347212676044876 1108 4 0.08286196585616779 1108 5 -0.039335966514023823 1108 6 0.07624971745515552 1108 7 0.06954242758087048 1108 8 0.03450353529586181 1108 9 0.029318828704546718 1108 10-11 0.06746714135068288 1108 12-13 0.08798580770402253 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96-97 0.9108758221102775 2316 98-99 0.873047247894565 2316 100-101 1.3870023434847027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 25.0 5 78.0 6 193.0 7 409.0 8 931.0 9 1809.0 10 3038.0 11 4786.0 12 7247.0 13 10241.0 14 12932.0 15 18157.0 16 24965.0 17 34430.0 18 30834.0 19 19568.0 20 17264.0 21 17915.0 22 18430.0 23 20040.0 24 22998.0 25 27240.0 26 32796.0 27 40524.0 28 50132.0 29 61325.0 30 75883.0 31 94317.0 32 119053.0 33 154621.0 34 208498.0 35 292772.0 36 415458.0 37 511081.0 38 262072.0 39 12516.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.354565111909935 19.276005371280423 15.472504586285243 21.8969249305244 2 16.24846309855334 20.787632701224073 38.670364501396215 24.293539698826372 3 19.925748657223448 24.375571277745667 29.856980056762954 25.84170000826793 4 13.608979356076626 16.796324618093895 35.476594891865346 34.11810113396413 5 13.866540770360414 37.77818930323406 33.0840174944044 15.271252432001134 6 32.97882838483136 35.80661274350862 16.009340855498305 15.205218016161707 7 29.087643483823623 33.45783489211114 19.278221168262274 18.176300455802963 8 27.59568223564819 34.304211805308164 18.452075917121054 19.648030041922596 9 27.003134574081606 16.47040176423141 19.530678781787238 36.995784879899745 10-11 25.746390210466437 26.72903555933687 25.63934528665141 21.885228943545282 12-13 26.821074274122243 24.01527856868107 26.892974765670886 22.2706723915258 14-15 23.450439476659103 25.05016956876079 25.93546671320076 25.56392424137935 16-17 22.18460801059514 28.616600097767314 26.539616147076185 22.65917574456136 18-19 22.290643256360305 26.418016618919687 29.68779230655807 21.603547818161935 20-21 23.05898915736443 25.145046326239218 30.090271156379515 21.705693360016838 22-23 22.82307774959098 25.056180973986436 30.182270968705538 21.938470307717044 24-25 22.71564151323688 25.38513502443611 30.041960695710085 21.85726276661692 26-27 22.259701534619722 25.64021231757411 30.200464154168376 21.899621993637787 28-29 22.19805731135351 26.0845967045505 30.086812696306826 21.630533287789167 30-31 22.230500735540932 25.515383492868814 30.023653137107804 22.230462634482446 32-33 22.138677184590865 25.65622405556049 30.000278137726944 22.204820622121705 34-35 22.7766984594599 25.508163342285812 29.803524271707794 21.911613926546494 36-37 22.78005591354832 25.309748674791756 29.812265736996768 22.09792967466316 38-39 22.41835800440372 25.65241394971194 29.873477815087178 22.055750230797162 40-41 22.577922155856314 25.783546042706472 29.610447801285574 22.028084000151644 42-43 22.409230189385344 25.83333762061309 29.268820634124882 22.488611555876687 44-45 22.59848075839395 25.842023867265056 29.24486750166406 22.314627872676933 46-47 23.02845120340288 26.337680537103004 28.48768516638161 22.14618309311251 48-49 23.08705063135359 26.352501848853866 28.18937292896934 22.37107459082321 50-51 22.944571723147043 26.83247993312502 28.035482753746383 22.187465589981556 52-53 23.16241452503792 26.9070627551104 27.55072298663529 22.379799733216387 54-55 22.822534032817966 26.763155057210646 27.738427851264134 22.675883058707257 56-57 23.105091482546477 27.044912384710962 27.626658396196905 22.22333773654566 58-59 23.266811425288207 26.451717005150122 27.722863568872807 22.558608000688867 60-61 22.83289752072602 26.53430104941745 28.357189041068747 22.27561238878777 62-63 22.575334365364004 26.732102694544956 28.39288973286967 22.29967320722137 64-65 22.611758977276146 26.8339849249352 28.124391573722306 22.429864524066343 66-67 22.78027995895754 26.758430525958442 27.859246307721676 22.602043207362343 68-69 22.6620904755355 26.711737678784452 27.967434263291267 22.658737582388778 70-71 22.982615630044815 26.735722295101077 27.668455257355507 22.613206817498597 72-73 22.922796968222574 26.600615941711474 27.754468396886534 22.722118693179418 74-75 22.677426151575915 27.505973293444065 27.067106251278766 22.74949430370125 76-77 22.900336394245368 26.91026324402318 27.434171848728127 22.75522851300332 78-79 22.889153733579874 27.01123104900977 27.378601454927022 22.721013762483334 80-81 22.899345766724746 27.253629983094562 27.22137743708658 22.625646813094114 82-83 22.87073136115416 27.1557013136325 27.230760584738395 22.74280674047495 84-85 22.841029867325968 27.23099089172197 27.10323762887963 22.824741612072437 86-87 22.93750397679798 27.18640066539689 27.0588764226459 22.817218935159236 88-89 22.789252758230877 27.117590153772063 26.95676558590474 23.13639150209232 90-91 22.956397416383115 26.969611579139702 26.98649223575533 23.087498768721858 92-93 22.88889866839345 26.914371356618588 27.12955220855187 23.067177766436092 94-95 22.75073258810203 27.01481254850741 27.100235121631915 23.134219741758645 96-97 22.592251235331567 26.95440332027864 27.186438766455375 23.266906677934422 98-99 22.636521393236517 27.06989824465214 26.780556656790168 23.513023705321174 100-101 22.630739408295028 26.782578011902082 27.16377307471439 23.42290950508851 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1472.0 1 1027.0 2 754.5 3 1009.0 4 922.0 5 849.5 6 1518.0 7 3415.0 8 5537.5 9 6427.5 10 5838.5 11 5068.0 12 5197.5 13 5526.0 14 5833.0 15 6048.0 16 6085.5 17 6023.0 18 6092.5 19 6324.5 20 6561.0 21 7093.5 22 8031.0 23 9439.5 24 11137.0 25 13250.5 26 15878.5 27 18466.5 28 20296.0 29 22810.0 30 26021.0 31 28557.0 32 32153.5 33 34506.0 34 36215.0 35 39264.5 36 44439.5 37 50327.0 38 54740.5 39 58030.5 40 61293.0 41 68104.0 42 74392.5 43 79085.5 44 85285.0 45 90622.5 46 94394.0 47 103352.0 48 115999.5 49 116516.5 50 108559.0 51 101926.5 52 98233.5 53 99862.5 54 101060.0 55 93869.5 56 82925.5 57 74762.0 58 68392.0 59 60800.5 60 50534.0 61 41119.0 62 33048.5 63 25092.0 64 18764.5 65 14537.5 66 11011.0 67 8704.5 68 6683.5 69 4717.0 70 3334.0 71 2327.0 72 1858.0 73 1612.5 74 1345.0 75 1035.5 76 677.0 77 444.5 78 301.5 79 214.5 80 142.0 81 94.0 82 58.0 83 24.5 84 11.5 85 8.5 86 5.5 87 3.0 88 2.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03871067542127388 2 0.0 3 0.0 4 3.8101058485505786E-5 5 0.008267929691354755 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005276996600242552 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.476568801557876E-4 22-23 0.0017145476318477604 24-25 0.0 26-27 0.0015240423394202313 28-29 0.0015430928686629842 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.381621725833166E-4 38-39 0.0 40-41 3.238589971267992E-4 42-43 0.021241340105669474 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.476568801557876E-4 84-85 3.238589971267992E-4 86-87 0.0 88-89 0.0 90-91 0.011182660665495949 92-93 0.0023813161553441118 94-95 0.0 96-97 0.0 98-99 7.429706404673629E-4 100-101 0.013411572586898037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2624599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.08656153042378 #Duplication Level Percentage of deduplicated Percentage of total 1 65.179827233844 24.82475500484191 2 15.914942391890923 12.122908653238069 3 6.739874017389987 7.700958794118846 4 3.6192820171754994 5.513840289724438 5 2.1111125999500127 4.020250996782453 6 1.3680614433545917 3.1262853803835013 7 0.9518756998013207 2.537757068685874 8 0.660376270086909 2.012116915511745 9 0.5105544358721802 1.7500736642828931 >10 2.617679266868757 17.98752779321445 >50 0.20575625270027287 5.432159987787805 >100 0.10965496206910223 7.5938574439392825 >500 0.008002479270183925 2.008545796999308 >1k 0.002600805762808697 1.4883507227911503 >5k 1.000309908772576E-4 0.2286663860695358 >10k+ 3.0009297263177277E-4 1.6519451016286355 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18091 0.6892862490612852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14720 0.5608475809066452 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10549 0.40192806596360053 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6002 0.22868255303000576 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3398 0.12946739673374866 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 0.10603524576516261 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.8101058485505786E-5 0.0 0.0 3.8101058485505786E-5 6 0.0 3.8101058485505786E-5 0.0 0.0 3.8101058485505786E-5 7 0.0 3.8101058485505786E-5 0.0 0.0 3.8101058485505786E-5 8 0.0 3.8101058485505786E-5 0.0 0.0 3.8101058485505786E-5 9 0.0 3.8101058485505786E-5 0.0 0.0 3.8101058485505786E-5 10-11 0.0 3.8101058485505786E-5 0.0 3.8101058485505786E-5 3.8101058485505786E-5 12-13 0.0 3.8101058485505786E-5 0.0 3.8101058485505786E-5 1.3335370469927025E-4 14-15 0.0 3.8101058485505786E-5 0.0 3.8101058485505786E-5 1.7145476318477604E-4 16-17 0.0 3.8101058485505786E-5 0.0 5.7151587728258675E-5 2.0955582167028183E-4 18-19 0.0 3.8101058485505786E-5 0.0 7.620211697101157E-5 2.2860635091303473E-4 20-21 0.0 3.8101058485505786E-5 0.0 7.620211697101157E-5 2.2860635091303473E-4 22-23 0.0 3.8101058485505786E-5 0.0 1.7145476318477604E-4 2.2860635091303473E-4 24-25 0.0 3.8101058485505786E-5 0.0 3.6196005561230495E-4 2.2860635091303473E-4 26-27 0.0 3.8101058485505786E-5 0.0 5.143642895543281E-4 2.2860635091303473E-4 28-29 0.0 3.8101058485505786E-5 0.0 0.0012192338715361851 2.2860635091303473E-4 30-31 0.0 3.8101058485505786E-5 0.0 0.002876629915655687 2.2860635091303473E-4 32-33 0.0 5.7151587728258675E-5 0.0 0.005391299775699068 2.2860635091303473E-4 34-35 0.0 7.620211697101157E-5 0.0 0.007925020164985204 2.2860635091303473E-4 36-37 0.0 7.620211697101157E-5 0.0 0.01238284400778938 2.2860635091303473E-4 38-39 0.0 7.620211697101157E-5 0.0 0.017869396429702214 2.2860635091303473E-4 40-41 0.0 7.620211697101157E-5 0.0 0.02507049648346281 2.2860635091303473E-4 42-43 0.0 7.620211697101157E-5 0.0 0.03573879285940443 2.476568801557876E-4 44-45 0.0 7.620211697101157E-5 0.0 0.04909321385857421 2.8575793864129336E-4 46-47 3.8101058485505786E-5 7.620211697101157E-5 0.0 0.07342073970156965 3.238589971267992E-4 48-49 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.10584474047273507 3.8101058485505787E-4 50-51 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.13998328887574826 3.8101058485505787E-4 52-53 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.1721977338252434 3.8101058485505787E-4 54-55 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.21132752088985784 3.8101058485505787E-4 56-57 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.2529148262267874 3.8101058485505787E-4 58-59 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.301588928442021 3.8101058485505787E-4 60-61 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.3643794728261346 3.8101058485505787E-4 62-63 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.4249982568765743 4.1911164334056366E-4 64-65 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.49110359334892684 4.1911164334056366E-4 66-67 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.5782788151637641 4.762632310688223E-4 68-69 3.8101058485505786E-5 1.1430317545651736E-4 0.0 0.6777606788694197 5.33414818797081E-4 70-71 3.8101058485505786E-5 1.3335370469927025E-4 0.0 0.7862343923776547 5.33414818797081E-4 72-73 3.8101058485505786E-5 1.7145476318477604E-4 0.0 0.898461060146712 5.52465348039834E-4 74-75 3.8101058485505786E-5 1.9050529242752893E-4 0.0 1.0151265012293307 5.905664065253397E-4 76-77 3.8101058485505786E-5 1.9050529242752893E-4 0.0 1.1395074066552642 6.096169357680926E-4 78-79 3.8101058485505786E-5 1.9050529242752893E-4 0.0 1.289701779205128 6.096169357680926E-4 80-81 3.8101058485505786E-5 1.9050529242752893E-4 0.0 1.4592705399948716 6.096169357680926E-4 82-83 3.8101058485505786E-5 1.9050529242752893E-4 0.0 1.6480422342613101 6.477179942535983E-4 84-85 3.8101058485505786E-5 1.9050529242752893E-4 0.0 1.847653679666875 6.477179942535983E-4 86-87 3.8101058485505786E-5 1.9050529242752893E-4 0.0 2.0792700142002642 6.858190527391042E-4 88-89 3.8101058485505786E-5 1.9050529242752893E-4 0.0 2.3649136496661014 7.239201112246099E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20400 0.0 29.070448 1 GGTATCA 14485 0.0 28.770567 1 TAAGGTG 1015 0.0 23.866991 5 ATCAACG 25060 0.0 23.560452 3 TATCAAC 25230 0.0 23.477007 2 TACATGG 12895 0.0 23.427681 2 GTACATG 13345 0.0 23.394518 1 TCAACGC 25365 0.0 23.277147 4 GAGTACT 14215 0.0 23.275156 12-13 CAACGCA 25660 0.0 22.991034 5 AACGCAG 26225 0.0 22.494421 6 AGTACTT 14620 0.0 22.126774 12-13 GTACTTT 15010 0.0 21.834398 14-15 ACCGTCG 265 7.930794E-10 21.51066 8 ACATGGG 13425 0.0 21.264427 3 AGAGTAC 22295 0.0 21.263826 10-11 GTAAGGT 985 0.0 20.736038 4 ACGCAGA 29225 0.0 20.0576 7 CGCAGAG 29325 0.0 19.908209 8 TACTTTT 16155 0.0 19.88995 14-15 >>END_MODULE