FastQCFastQC Report
Thu 26 May 2016
SRR937540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259857
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154460.6834945750992208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113730.503261932060303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80850.35776600023806815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40560.17948038305078595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31790.14067261778068257No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27470.12155636396462254No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.11275049704472451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA156850.031.9001981
GGTATCA107400.030.3795951
ATCAACG190700.025.951913
TATCAAC191650.025.8982622
TCAACGC193300.025.5782494
CAACGCA195550.025.3297585
TACATGG112050.025.1182162
AACGCAG198500.025.0068476
GTACATG115350.024.9459441
GAGTACT111150.023.88676312-13
ACATGGG115150.023.615733
GTACTTT116650.022.51496514-15
AGTACTT114350.022.38760212-13
CGCAGAG221900.022.1776338
ACGCAGA223200.022.1123097
AGAGTAC176750.021.32271410-11
CATGGGG54100.020.738824
GCAGAGT230350.020.6831119
CATGGGA64950.020.4218164
TACTTTT125950.020.21145214-15
Produced by FastQC (version 0.11.5)