FastQCFastQC Report
Thu 26 May 2016
SRR937540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259857
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154930.6855743527134681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126080.5579114076687154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90900.40223784071293006No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52630.23289084220815742No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28540.1262911768311004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.10536064892601611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126550.029.1806581
GTATCAA176200.027.9171241
TAAGGTG10000.023.7527545
TATCAAC213650.023.1468472
ATCAACG213350.023.0012633
GAGTACT125700.022.95656612-13
TCAACGC215700.022.750674
GTACATG111350.022.5361611
TACATGG109400.022.4500452
CAACGCA219000.022.4078485
AACGCAG222400.022.0613776
GTACTTT129750.022.05549414-15
ACATGGG110750.021.7903373
AGTACTT126900.021.61654712-13
GTAAGGT9300.021.4541024
GGACCGA5350.021.307116
AGGACCG7650.020.4925735
AGAGTAC192100.020.33782410-11
TACTTTT138000.019.70441214-15
ACGCAGA251500.019.4722737
Produced by FastQC (version 0.11.5)