FastQCFastQC Report
Thu 26 May 2016
SRR937539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760537
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114370.6496313340759098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87790.49865467184160284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63060.3581861670615272No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22690.1288811311548692No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.1147945200810889No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.10644479496880782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.053.7957341
GTATCAA75750.046.2647131
ATCAACG95150.036.4747663
TATCAAC97550.035.772332
TCAACGC97550.035.5804254
CAACGCA99250.034.9709855
AACGCAG101450.034.3687636
ACGCAGA116400.029.7513567
CGCAGAG117650.029.4335828
GCAGAGT126100.026.8585139
GTACATG70400.025.802141
TACATGG71200.024.8394152
GTGGTAT19050.024.2126181
ACATGGG72200.024.0344453
GAGTACT78150.023.64540312-13
TGGTATC18250.023.1849172
AGTACTT81700.022.35632112-13
CAGAGTA120350.022.26142310-11
AGAGTAC116100.022.0943610-11
GTACTTT85050.021.55829214-15
Produced by FastQC (version 0.11.5)