FastQCFastQC Report
Thu 26 May 2016
SRR937539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760537
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113530.6448600625831777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101000.5736885961499247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74890.4253815739175036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26010.1477390137213816No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24790.14080930988669935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.12410985966213718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51750.054.899661
TTCGCGA250.001614825138.00691290-91
GTATCAA85050.037.035481
TATCAAC116600.026.971262
ATCAACG115900.026.9702053
TCAACGC118050.026.4790084
CAACGCA121250.025.819365
AACGCAG124550.025.2453866
TAAGGTG9500.024.5027185
GTAAGGT8500.023.473194
AGGTAAG8700.022.9335782
AGTACTT99300.021.95551112-13
ACGCAGA143000.021.9223967
TGGTATC23550.021.5841352
CGCAGAG145050.021.5470718
GAGTACT99050.021.38752412-13
GGTAAGG10050.021.2710153
GTGGTAT25650.020.9300331
AGAGTAC139350.020.57076510-11
AAGGTAA10400.020.5569171
Produced by FastQC (version 0.11.5)