##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1760537 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193906745498676 31.0 31.0 33.0 30.0 34.0 2 31.540390801215764 31.0 31.0 34.0 30.0 34.0 3 31.59269643296335 31.0 31.0 34.0 30.0 34.0 4 35.460302737176214 37.0 35.0 37.0 33.0 37.0 5 35.30069120955709 37.0 35.0 37.0 33.0 37.0 6 35.386390061668685 37.0 35.0 37.0 33.0 37.0 7 35.29677024680538 37.0 35.0 37.0 32.0 37.0 8 35.25031169467043 37.0 35.0 37.0 32.0 37.0 9 36.78704679310915 39.0 37.0 39.0 32.0 39.0 10-11 36.642892765105195 38.0 35.0 39.0 32.0 39.0 12-13 36.4801037978753 38.0 35.0 39.0 32.0 39.0 14-15 37.35566818533209 39.0 36.0 40.0 32.0 41.0 16-17 37.246427936476195 39.0 36.0 40.0 32.0 41.0 18-19 37.18392655195545 39.0 36.0 40.0 31.5 41.0 20-21 37.01917937538376 39.0 36.0 40.0 31.0 41.0 22-23 36.9178256406994 39.0 36.0 40.0 31.0 41.0 24-25 36.91118874525216 39.0 36.0 40.0 31.0 41.0 26-27 36.63715133507561 38.0 35.5 40.0 30.0 41.0 28-29 36.386407669932524 38.0 35.0 40.0 30.0 41.0 30-31 36.373421859353144 38.0 35.0 40.0 30.0 41.0 32-33 36.20939690560323 38.0 35.0 40.0 30.0 41.0 34-35 35.958922192490135 38.0 35.0 40.0 30.0 41.0 36-37 35.687844390660345 38.0 34.0 40.0 29.0 41.0 38-39 35.4587583220347 38.0 34.0 40.0 28.0 41.0 40-41 35.147647564351104 38.0 34.0 40.0 27.0 41.0 42-43 34.79954525238606 38.0 33.0 40.0 26.0 41.0 44-45 34.332789654520184 37.0 33.0 40.0 24.5 41.0 46-47 33.80752321592787 37.0 33.0 40.0 23.5 41.0 48-49 33.310307593648986 37.0 32.0 39.0 22.5 40.0 50-51 32.872496573488654 36.5 31.0 39.0 19.5 40.0 52-53 32.456100326207284 36.0 31.0 39.0 18.5 40.0 54-55 32.04631740201995 35.5 30.5 39.0 17.0 40.0 56-57 31.573949880065 35.0 30.0 38.5 13.5 40.0 58-59 31.12485309879883 35.0 29.0 38.0 10.5 40.0 60-61 30.591853792337226 34.0 28.0 38.0 8.0 40.0 62-63 29.98382340160985 34.0 28.0 38.0 7.0 40.0 64-65 29.341379647232635 34.0 26.5 37.0 4.0 39.0 66-67 28.708147570883202 33.0 26.0 36.5 2.0 39.0 68-69 28.06629198931917 32.5 26.0 36.0 2.0 39.0 70-71 27.493630352557204 32.0 25.0 35.5 2.0 38.0 72-73 26.78791442610976 31.0 23.5 35.0 2.0 37.5 74-75 26.12311896881463 31.0 22.0 34.5 2.0 37.0 76-77 25.372436648590742 30.0 21.5 34.0 2.0 36.0 78-79 25.89662216698655 31.0 22.0 34.0 2.0 36.0 80-81 25.565940392050834 31.0 21.0 34.0 2.0 36.0 82-83 24.972014788669593 31.0 19.5 34.0 2.0 35.0 84-85 24.27324361828238 30.0 17.5 34.0 2.0 35.0 86-87 23.51979935667356 30.0 9.5 34.0 2.0 35.0 88-89 22.662162169837952 29.0 4.5 34.0 2.0 35.0 90-91 21.616738245205866 29.0 2.0 33.0 2.0 35.0 92-93 20.659746145636248 27.0 2.0 33.0 2.0 34.0 94-95 19.63520533791678 26.0 2.0 32.5 2.0 34.0 96-97 18.452714995481493 25.0 2.0 32.0 2.0 34.0 98-99 16.92030670187562 20.5 2.0 32.0 2.0 34.0 100-101 14.406273483601879 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25829322150713097 1101 2 0.21282206787991598 1101 3 0.17599686222424182 1101 4 0.13158946202683808 1101 5 0.018133445749796806 1101 6 0.08520623370907288 1101 7 0.20172509263444738 1101 8 0.0036122825583646545 1101 9 -0.02745428971236663 1101 10-11 0.23016657287236342 1101 12-13 0.1694907000562509 1101 14-15 0.23989967610261687 1101 16-17 0.11088010794635039 1101 18-19 0.2666990594905272 1101 20-21 0.12014957219349753 1101 22-23 0.2114741717022568 1101 24-25 0.12716650550294162 1101 26-27 -0.06618771519525524 1101 28-29 -0.1972460749774072 1101 30-31 -0.17848556801974524 1101 32-33 -0.06053651528075932 1101 34-35 -0.13396545730187626 1101 36-37 -0.3373222892464369 1101 38-39 -0.1217125505702299 1101 40-41 -0.054771066777362876 1101 42-43 0.10356077378740025 1101 44-45 -0.07393149985821879 1101 46-47 -0.3851032995491863 1101 48-49 -0.2736288727978433 1101 50-51 -0.1772039286931104 1101 52-53 -0.2634238359638701 1101 54-55 -0.34577108240596743 1101 56-57 -0.292706516076926 1101 58-59 -0.3274129911585959 1101 60-61 -0.12567324143666525 1101 62-63 -0.6069840964037923 1101 64-65 -0.6353147333061777 1101 66-67 -0.5014628481420083 1101 68-69 -0.45674896821686417 1101 70-71 -0.3879702916451073 1101 72-73 -0.4685041371695462 1101 74-75 -0.5895561230534163 1101 76-77 -0.3947074247757918 1101 78-79 -0.2654640546675111 1101 80-81 -0.1794521647092715 1101 82-83 -0.2092730187999905 1101 84-85 -0.35373226056050555 1101 86-87 -0.4576607159400261 1101 88-89 -0.5878369295173478 1101 90-91 -0.7208222156550548 1101 92-93 -0.6322464700135519 1101 94-95 -0.8216379063562904 1101 96-97 -0.7526986141032914 1101 98-99 -1.0626255874655257 1101 100-101 -0.843159007263834 1102 1 0.26055372390687026 1102 2 0.1836495346610043 1102 3 0.19212482306152978 1102 4 0.12250028199395047 1102 5 0.1418898394532775 1102 6 0.09310771414664742 1102 7 0.12638460540713936 1102 8 0.11294426049904871 1102 9 0.018841111018858214 1102 10-11 0.15461565790658938 1102 12-13 0.20623473705345674 1102 14-15 0.3338187147605183 1102 16-17 0.3023781132644956 1102 18-19 0.2628624587391286 1102 20-21 0.25827336602061735 1102 22-23 0.3530477903604492 1102 24-25 0.26456142957476914 1102 26-27 0.12485708611444579 1102 28-29 0.1272605450035158 1102 30-31 0.2821556815726538 1102 32-33 0.39291697715974294 1102 34-35 0.22456456916766143 1102 36-37 0.08588619173792722 1102 38-39 0.21582133154132777 1102 40-41 0.2523828921094946 1102 42-43 0.3725619658145618 1102 44-45 0.12928511261654307 1102 46-47 -0.031804214547420884 1102 48-49 0.14447459879463054 1102 50-51 0.08030801700290624 1102 52-53 0.08604405773993307 1102 54-55 -0.03686153950262039 1102 56-57 0.1639731743898203 1102 58-59 0.14346932748028252 1102 60-61 0.0666518693110767 1102 62-63 -0.1693717555796681 1102 64-65 -0.35159182195564753 1102 66-67 -0.2412140435771235 1102 68-69 0.08622665094376813 1102 70-71 -0.0015864485140610896 1102 72-73 -0.31432108256371194 1102 74-75 -0.4170824081285822 1102 76-77 -0.16247536960975495 1102 78-79 -0.14480965793949352 1102 80-81 -0.0961578335351767 1102 82-83 -0.3049635452674728 1102 84-85 -0.24829984940915395 1102 86-87 -0.5154165847101488 1102 88-89 -0.5728648606891937 1102 90-91 -0.18029751165493835 1102 92-93 -0.15435890918154271 1102 94-95 -0.6617321909658109 1102 96-97 -0.6218465321453088 1102 98-99 -0.5260029386033196 1102 100-101 -0.49785087505938996 1103 1 0.31597382779857597 1103 2 0.24576698627284443 1103 3 0.2705757427630324 1103 4 0.15065329842880004 1103 5 0.09785476776333013 1103 6 0.07456912356432355 1103 7 0.14969765853909678 1103 8 0.20458523793716665 1103 9 0.132974921965328 1103 10-11 0.24070513360435086 1103 12-13 0.32770609257276817 1103 14-15 0.257691924493507 1103 16-17 0.1861715372391899 1103 18-19 0.2747412498038315 1103 20-21 0.16373825657386476 1103 22-23 0.019077806393532626 1103 24-25 0.11981383902207199 1103 26-27 0.20374116605206893 1103 28-29 -0.02209295838979841 1103 30-31 0.16933449320541882 1103 32-33 0.1986109743286093 1103 34-35 0.221092403441574 1103 36-37 0.07724739334715736 1103 38-39 0.16493183548410428 1103 40-41 0.2701160737480848 1103 42-43 0.4528602570793012 1103 44-45 0.2802065663661324 1103 46-47 0.3731195646875136 1103 48-49 0.07071136357475183 1103 50-51 0.03390845156076239 1103 52-53 0.05629107067613148 1103 54-55 0.019379827018319418 1103 56-57 0.13619845609521164 1103 58-59 0.06040696700836179 1103 60-61 0.16732350235333016 1103 62-63 -0.09448249148688603 1103 64-65 0.14742524954322178 1103 66-67 0.07032975494088944 1103 68-69 -0.06173329263380012 1103 70-71 -0.2066357221858084 1103 72-73 -0.35591948451221 1103 74-75 -0.45795519106367166 1103 76-77 -0.29932855488635113 1103 78-79 -0.4040604920310926 1103 80-81 -0.4059811942745277 1103 82-83 -0.5159365693871862 1103 84-85 -0.4380471875544991 1103 86-87 -0.47459897677262575 1103 88-89 -0.586342370776098 1103 90-91 -0.24544969243368087 1103 92-93 -0.47159053027512243 1103 94-95 -0.4372481762786222 1103 96-97 -0.7041303932186302 1103 98-99 -0.4810041297157426 1103 100-101 -0.37311845815251843 1104 1 0.28921967345629795 1104 2 0.24306657081795535 1104 3 0.14647481375400062 1104 4 0.12237972134320074 1104 5 -0.012236645535359969 1104 6 0.0964815383679749 1104 7 0.026317391282674407 1104 8 0.1325244345811356 1104 9 -0.02122437902582419 1104 10-11 0.1345060788943897 1104 12-13 0.13648196759415754 1104 14-15 0.1056742368755863 1104 16-17 0.10078696254119279 1104 18-19 0.24787077305136762 1104 20-21 0.16918185611402947 1104 22-23 0.15191014757728993 1104 24-25 0.01948317759176632 1104 26-27 0.12237622277525162 1104 28-29 0.10632078410089463 1104 30-31 0.039045129329913664 1104 32-33 0.20208039868350625 1104 34-35 0.2124694624308816 1104 36-37 0.29464695318150547 1104 38-39 0.08444457888305834 1104 40-41 -0.04522013727357432 1104 42-43 0.21640468976065108 1104 44-45 0.16254985755892903 1104 46-47 -0.27030472739868117 1104 48-49 -0.10913878506173802 1104 50-51 0.035169609627835996 1104 52-53 0.11913327137215646 1104 54-55 -0.18518955692332284 1104 56-57 -0.05362380822070989 1104 58-59 -0.05784478361292145 1104 60-61 -0.19493382182627883 1104 62-63 -0.07232792772278174 1104 64-65 -0.2508777655832368 1104 66-67 -0.27359800863577277 1104 68-69 -0.37698954491169445 1104 70-71 -0.35303604978938097 1104 72-73 -0.4090370903945306 1104 74-75 -0.35708630604806757 1104 76-77 -0.4441943770895094 1104 78-79 -0.5537560702919038 1104 80-81 -0.38527539203832717 1104 82-83 -0.5202783085125908 1104 84-85 -0.32702126935125975 1104 86-87 -0.432589231310029 1104 88-89 -0.5707169446393827 1104 90-91 -0.6045320139893136 1104 92-93 -0.4752510704904438 1104 94-95 -0.5849241247711419 1104 96-97 -0.5582695288708095 1104 98-99 -0.25779609400662196 1104 100-101 -0.25135306826376613 1105 1 0.2517020547733999 1105 2 0.20135158196988456 1105 3 0.17521047533207934 1105 4 0.08581448117715951 1105 5 0.025028227360785138 1105 6 0.08221504031215687 1105 7 0.12398019601076982 1105 8 0.1333063269011987 1105 9 0.08952579859948173 1105 10-11 0.06408281281359507 1105 12-13 0.05436729090630621 1105 14-15 0.10075592320570337 1105 16-17 0.10001806979363437 1105 18-19 0.056511418409819214 1105 20-21 -0.08304916887882285 1105 22-23 0.022446339452677933 1105 24-25 0.04299354379185161 1105 26-27 0.06813529338679558 1105 28-29 -0.11141640801701413 1105 30-31 0.04464080597045239 1105 32-33 0.0966801844676013 1105 34-35 0.10150969333738402 1105 36-37 0.05057838107585866 1105 38-39 0.22940941823605243 1105 40-41 0.11781815690973474 1105 42-43 0.04380766073835929 1105 44-45 0.17830192971209868 1105 46-47 0.04339871868143064 1105 48-49 0.0978127043696162 1105 50-51 0.22324870328912283 1105 52-53 0.3691299682020954 1105 54-55 0.28444781041178047 1105 56-57 0.2057359905373808 1105 58-59 0.17070187270523363 1105 60-61 0.01977212571256004 1105 62-63 -0.20294699293844332 1105 64-65 -0.17792235326450268 1105 66-67 -0.16552915267061152 1105 68-69 -0.1265484118774829 1105 70-71 -0.17184854097205005 1105 72-73 0.09975307135850997 1105 74-75 -0.05322620020170987 1105 76-77 -0.22522787293880953 1105 78-79 -0.011617103583169808 1105 80-81 -0.2760902776996268 1105 82-83 -0.3373168908175721 1105 84-85 0.10892632063454855 1105 86-87 -0.02579158436051543 1105 88-89 -0.16585687331648202 1105 90-91 0.0011893535998090954 1105 92-93 -0.02373105818134036 1105 94-95 -0.11536603351755303 1105 96-97 0.1659497615571155 1105 98-99 0.13094163045444063 1105 100-101 0.003402537642047676 1106 1 0.033771458335060345 1106 2 0.02838019754847565 1106 3 0.04947896975543031 1106 4 -0.04122361446722067 1106 5 0.012228716532185047 1106 6 -0.09294955733015087 1106 7 -0.08225057723079487 1106 8 -0.1941799388534875 1106 9 -0.052225122151909886 1106 10-11 -0.2961169084724702 1106 12-13 -0.4681949101753631 1106 14-15 -0.20329763442598647 1106 16-17 -0.0465721875427505 1106 18-19 -0.10861781858708497 1106 20-21 -0.09536827737343145 1106 22-23 0.03595839572635384 1106 24-25 -0.09479103593312743 1106 26-27 0.0444105992374233 1106 28-29 -0.039166101099887385 1106 30-31 -0.09511183541287949 1106 32-33 -0.08364263547277062 1106 34-35 -0.5089174310268589 1106 36-37 -0.050355523905444954 1106 38-39 -0.06097969106436807 1106 40-41 -0.07120546741421663 1106 42-43 -0.26416187507022215 1106 44-45 -0.16228637947077118 1106 46-47 -0.07867040047825213 1106 48-49 -0.07997727899287099 1106 50-51 0.4170648563732229 1106 52-53 0.3385488164689008 1106 54-55 0.27085315906490237 1106 56-57 0.22692073805952973 1106 58-59 0.2731717853464275 1106 60-61 0.2863332081464698 1106 62-63 -0.27238503833847716 1106 64-65 -0.5749502341379973 1106 66-67 -0.5560960132046198 1106 68-69 0.04500285868833842 1106 70-71 -0.25102616896478835 1106 72-73 -0.432767583334833 1106 74-75 -0.5826642930020789 1106 76-77 -0.49594347570509356 1106 78-79 -0.6177894559192936 1106 80-81 -0.8329421137513684 1106 82-83 -1.176771028629286 1106 84-85 -0.9958044691971288 1106 86-87 -0.9025270289044975 1106 88-89 -1.020151343120105 1106 90-91 -1.0995606381051317 1106 92-93 -1.2756068076990452 1106 94-95 -1.266696205248948 1106 96-97 -0.8612599232780944 1106 98-99 -1.1918491587136373 1106 100-101 -1.6134935466167786 1107 1 0.10457714837151855 1107 2 0.11816263733091503 1107 3 0.038320043211889754 1107 4 0.013070809057971644 1107 5 -0.010426833046444983 1107 6 -0.08404692150444504 1107 7 -0.1547686574411813 1107 8 -0.07977852829822751 1107 9 -0.09594934664536936 1107 10-11 0.014811906302497846 1107 12-13 0.008775982180139863 1107 14-15 4.5587701983862416E-4 1107 16-17 0.09887638098273754 1107 18-19 0.10877653919188646 1107 20-21 0.13430568114667807 1107 22-23 0.14500746631388495 1107 24-25 -0.09051902589188643 1107 26-27 0.018848767906362696 1107 28-29 0.1487156752434302 1107 30-31 0.018264531809641937 1107 32-33 -0.1804255534095347 1107 34-35 -0.0670101929657747 1107 36-37 0.05452165020250277 1107 38-39 0.12691403913705557 1107 40-41 -0.07665941517586106 1107 42-43 0.11050042843823604 1107 44-45 -0.0684605471833919 1107 46-47 0.1128199467813289 1107 48-49 -0.22821557915425217 1107 50-51 -0.08296801673657228 1107 52-53 -0.006868373179052867 1107 54-55 -0.04872875474778127 1107 56-57 -0.23472530820189874 1107 58-59 0.016319236186184582 1107 60-61 -0.04428809362867625 1107 62-63 -0.012300464792367904 1107 64-65 -0.024696214653207704 1107 66-67 -0.2480973878983015 1107 68-69 -0.17662167062849932 1107 70-71 -0.06579516065685453 1107 72-73 -0.06313785408972095 1107 74-75 -0.48831249095796636 1107 76-77 -0.06398931875093794 1107 78-79 -0.26907001296908106 1107 80-81 0.00686273951502514 1107 82-83 -0.027119184746677405 1107 84-85 -0.1794725105574777 1107 86-87 -0.4781650660809973 1107 88-89 -0.4367149077605923 1107 90-91 -0.4222400953999781 1107 92-93 -0.5550118044117305 1107 94-95 -0.7234889851186495 1107 96-97 -0.8692630312812035 1107 98-99 -0.7810159748035304 1107 100-101 -0.6055711271558977 1108 1 0.11748162501736203 1108 2 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64-65 -0.20731075206898097 2316 66-67 0.04556234894031874 2316 68-69 0.0910421102644996 2316 70-71 0.3098832609043711 2316 72-73 0.5685263967343168 2316 74-75 0.21130560734366455 2316 76-77 0.3803081343552428 2316 78-79 0.3340773666690069 2316 80-81 0.3738548830624069 2316 82-83 0.2961172501314735 2316 84-85 0.1804623940663781 2316 86-87 0.3124202628045367 2316 88-89 0.6731728382913253 2316 90-91 0.8335074589730311 2316 92-93 1.123181410048904 2316 94-95 1.3892059369786978 2316 96-97 1.1287687188478621 2316 98-99 1.6211101321818937 2316 100-101 1.7195508232564496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 49.0 5 99.0 6 257.0 7 668.0 8 1417.0 9 2361.0 10 3594.0 11 5496.0 12 7767.0 13 10890.0 14 14168.0 15 18643.0 16 24250.0 17 30003.0 18 28504.0 19 23736.0 20 23839.0 21 25247.0 22 27476.0 23 30122.0 24 35143.0 25 40525.0 26 48597.0 27 57913.0 28 68124.0 29 79464.0 30 93285.0 31 107150.0 32 126544.0 33 147439.0 34 170772.0 35 188708.0 36 175103.0 37 114760.0 38 27777.0 39 623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.80647534450544 21.927514817270236 16.790766453234347 20.475243384989977 2 18.025011686775112 22.501600363979854 36.48824193981723 22.985146009427808 3 21.049429804656192 24.85395081159896 30.552382596900834 23.544236786844014 4 15.688111070656282 18.54740911437817 34.61960754019938 31.144872274766165 5 14.120983921196306 37.85623905196478 33.44804800402723 14.574729022811686 6 32.77210305719221 36.93072056991702 15.565705236527263 14.731471136363508 7 28.61751840489578 33.66364921611986 19.54619528019008 18.17263709879429 8 27.326207855898517 35.59590056897412 18.3337811133762 18.74411046175116 9 26.511399646812308 16.519391526562636 19.554204200195734 37.41500462642932 10-11 25.779577481188976 27.061402288051884 25.843762442936445 21.31525778782269 12-13 26.460341240707475 24.20695971996393 27.2112980069441 22.121401032384497 14-15 23.152736920610018 25.46135071287908 25.6657201751511 25.720192191359796 16-17 22.16374890161354 28.895132564666348 26.505776362553018 22.43534217116709 18-19 22.320150045128276 26.593079270699793 29.723288973761985 21.36348171040995 20-21 22.750331290964063 25.60014359255159 30.264203478824925 21.38532163765942 22-23 22.603550699180047 25.478202117377958 30.323741221731115 21.594505961710887 24-25 22.596622727321783 25.941263242438513 30.04930893087491 21.412805099364796 26-27 22.192789555104742 26.135242774062483 30.04281984452849 21.629147826304283 28-29 22.258136617526645 26.190483628782168 30.06321735107399 21.488162402617192 30-31 22.179425936518232 26.12677268356189 30.035352849727097 21.658448530192775 32-33 22.121545869243306 26.068267806924815 29.903518074314828 21.90666824951705 34-35 22.668140459416644 26.05398239287218 29.782645863165612 21.495231284545568 36-37 22.703044582420024 25.90658418425742 29.67367343032268 21.716697802999878 38-39 22.384164604322432 26.114475299297883 29.731354694618744 21.77000540176094 40-41 22.573121737120164 26.036524373723037 29.729347472313627 21.661006416843176 42-43 22.517678322695286 26.134463185688283 29.413218295473392 21.934640196143036 44-45 22.572629828285347 26.31529470837591 29.32562621518321 21.786449248155535 46-47 22.827637249316542 26.70935061290958 28.63401905214148 21.828993085632394 48-49 22.904403599583535 26.871999850045754 28.21519797652648 22.008398573844232 50-51 22.857088490613943 27.147654380453233 28.116023690498977 21.879233438433843 52-53 23.299425118585976 26.965039644154025 27.84428841881767 21.89124681844233 54-55 23.06479390367479 27.121853799070283 27.8762263310719 21.93712596618303 56-57 22.99829540645837 27.301300682689426 27.796263299209272 21.904140611642926 58-59 23.055806268201124 26.91505489518255 27.82594174391109 22.20319709270524 60-61 22.87057869275113 27.057284226346844 28.05927964024613 22.012857440655893 62-63 22.830732895701708 27.107751782552707 28.280064548487193 21.78145077325839 64-65 22.678023807508733 27.030247021221363 28.132524337744673 22.159204833525227 66-67 22.70491901050645 27.172192348130142 27.971238321035003 22.1516503203284 68-69 22.90289837702928 26.962682408833217 28.036360496825687 22.09805871731182 70-71 22.862512971894372 27.113744272344174 27.83227503880918 22.19146771695227 72-73 22.968332957501037 27.160093766845 27.614301772697765 22.2572715029562 74-75 22.894690654044762 27.590019408850825 27.277273922672457 22.23801601443196 76-77 22.929111969813757 27.3453781431461 27.647672272721337 22.077837614318813 78-79 22.95666038259917 27.37244374869713 27.519955559014097 22.150940309689602 80-81 23.033114328185096 27.540773070943697 27.332001542711115 22.094111058160095 82-83 23.080606421896668 27.414913802627456 27.320282859047325 22.18419691642855 84-85 23.02230030448105 27.456829210868396 27.28940865469134 22.23146182995922 86-87 23.269661472607506 27.459292250035073 27.131522938739717 22.139523338617707 88-89 23.04944457287748 27.484455027074127 27.001619392264974 22.46448100778342 90-91 23.096587205307635 27.378252334858523 27.097282528887312 22.42787793094653 92-93 23.194620648170318 27.188152066205834 27.160461218755138 22.45676606686871 94-95 23.075572964385298 27.086252660409865 27.18105328090236 22.657121094302475 96-97 23.26826985175546 26.895316599423925 27.113375066812 22.723038482008615 98-99 23.000406983299484 27.070154775777194 26.87700101305613 23.052437227867195 100-101 23.577726287958 26.543664891602948 27.24440176034964 22.634207060089416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2301.0 1 1473.5 2 848.5 3 1127.0 4 977.0 5 885.5 6 1667.5 7 3385.5 8 5035.5 9 5441.5 10 4409.5 11 3293.0 12 3068.0 13 3208.0 14 3321.0 15 3343.0 16 3430.5 17 3546.0 18 3642.0 19 3812.5 20 4075.0 21 4559.5 22 5270.0 23 6001.5 24 7063.0 25 8398.0 26 10018.0 27 11836.5 28 13473.5 29 15660.5 30 18362.5 31 21522.5 32 25280.0 33 27429.0 34 28456.5 35 30607.0 36 34375.5 37 38334.0 38 41725.5 39 44049.0 40 45970.5 41 49602.0 42 52971.0 43 55744.0 44 59319.0 45 62116.0 46 62598.0 47 63800.5 48 65976.5 49 66034.0 50 64955.5 51 63534.0 52 62336.0 53 62270.0 54 62220.0 55 58845.0 56 53979.5 57 49370.5 58 45109.5 59 40729.0 60 34421.0 61 28589.0 62 23299.0 63 17758.0 64 13339.0 65 10274.0 66 7913.0 67 6080.0 68 4466.0 69 3284.5 70 2499.0 71 1849.0 72 1374.0 73 1112.5 74 874.5 75 670.5 76 522.0 77 361.0 78 262.5 79 200.0 80 127.5 81 77.0 82 56.0 83 34.5 84 19.0 85 15.5 86 9.5 87 3.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014768221287027764 2 0.0 3 0.0 4 0.0 5 0.02942284087184762 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0028116421296456705 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.964089365915058E-4 24-25 2.2720340441581175E-4 26-27 0.0012212182987349884 28-29 0.001334820000942894 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.952119154553413E-4 42-43 0.010735360858647106 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.680085110395294E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.668114899033647E-4 84-85 2.840042555197647E-5 86-87 0.0 88-89 0.0 90-91 0.01167257490186233 92-93 0.0015620234053587059 94-95 0.0 96-97 0.0 98-99 0.0012780191498389412 100-101 0.01809107107660901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1760537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.169872593017814 #Duplication Level Percentage of deduplicated Percentage of total 1 72.09058223496689 35.44684743649795 2 14.0143339764901 13.781660322000377 3 5.415492180454024 7.988371816242257 4 2.695604305547641 5.301700810598711 5 1.5147493728178643 3.7240016835904015 6 0.933420500524115 2.753770025188899 7 0.634582758595371 2.1841647372902124 8 0.4457473851779308 1.7533873710295755 9 0.3471828603783996 1.5363843310156864 >10 1.7697577440147554 15.434180514671302 >50 0.09278371844731899 3.103057797371828 >100 0.04172887158276664 3.6633837497227346 >500 0.002881493573495794 1.0143345845690277 >1k 8.068182005788224E-4 0.6745228643167152 >5k 1.1525974293983177E-4 0.42442461190286335 >10k+ 2.3051948587966354E-4 1.2158073439914712 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11353 0.6448600625831777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10100 0.5736885961499247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7489 0.4253815739175036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2601 0.1477390137213816 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2479 0.14080930988669935 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2185 0.12410985966213718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.6800851103952945E-5 2 0.0 0.0 0.0 0.0 5.6800851103952945E-5 3 0.0 0.0 0.0 0.0 5.6800851103952945E-5 4 0.0 0.0 0.0 0.0 5.6800851103952945E-5 5 0.0 0.0 0.0 0.0 5.6800851103952945E-5 6 0.0 0.0 0.0 0.0 5.6800851103952945E-5 7 0.0 0.0 0.0 0.0 5.6800851103952945E-5 8 0.0 0.0 0.0 0.0 5.6800851103952945E-5 9 0.0 0.0 0.0 0.0 5.6800851103952945E-5 10-11 0.0 0.0 0.0 0.0 5.6800851103952945E-5 12-13 0.0 0.0 0.0 2.8400425551976472E-5 5.6800851103952945E-5 14-15 0.0 0.0 0.0 5.6800851103952945E-5 5.6800851103952945E-5 16-17 0.0 0.0 0.0 1.1360170220790589E-4 5.6800851103952945E-5 18-19 0.0 0.0 0.0 1.4200212775988236E-4 5.6800851103952945E-5 20-21 0.0 0.0 0.0 2.2720340441581178E-4 5.6800851103952945E-5 22-23 0.0 0.0 0.0 2.840042555197647E-4 5.6800851103952945E-5 24-25 0.0 0.0 0.0 3.976059577276706E-4 5.6800851103952945E-5 26-27 0.0 0.0 0.0 5.39608085487553E-4 5.6800851103952945E-5 28-29 0.0 0.0 0.0 9.088136176632471E-4 5.6800851103952945E-5 30-31 0.0 0.0 0.0 0.001675625107566612 5.6800851103952945E-5 32-33 0.0 0.0 0.0 0.0031524472362693882 5.6800851103952945E-5 34-35 0.0 0.0 0.0 0.005594883833739364 5.6800851103952945E-5 36-37 0.0 0.0 0.0 0.008804131921112707 5.6800851103952945E-5 38-39 0.0 0.0 0.0 0.013575403413844753 5.6800851103952945E-5 40-41 0.0 0.0 0.0 0.018772681289856447 5.6800851103952945E-5 42-43 0.0 0.0 0.0 0.025503582145674872 5.6800851103952945E-5 44-45 0.0 0.0 0.0 0.033626103853540146 5.6800851103952945E-5 46-47 0.0 0.0 0.0 0.044929473223226775 5.6800851103952945E-5 48-49 0.0 0.0 0.0 0.05651684684843318 5.6800851103952945E-5 50-51 0.0 0.0 0.0 0.07131346856101292 5.6800851103952945E-5 52-53 0.0 0.0 0.0 0.08684850133794406 5.6800851103952945E-5 54-55 0.0 0.0 0.0 0.10278114007260285 1.1360170220790589E-4 56-57 0.0 0.0 0.0 0.12402465838548125 1.1360170220790589E-4 58-59 0.0 0.0 0.0 0.14700060265703022 1.1360170220790589E-4 60-61 0.0 0.0 0.0 0.1754010282090067 1.1360170220790589E-4 62-63 0.0 0.0 0.0 0.2049658712086142 1.1360170220790589E-4 64-65 0.0 0.0 0.0 0.23927358527540177 1.1360170220790589E-4 66-67 0.0 0.0 0.0 0.280028195942488 1.1360170220790589E-4 68-69 0.0 0.0 0.0 0.329473336828479 1.1360170220790589E-4 70-71 0.0 0.0 0.0 0.3830933402706106 1.1360170220790589E-4 72-73 0.0 0.0 0.0 0.4423934288231375 1.1360170220790589E-4 74-75 0.0 0.0 0.0 0.5059535812084608 1.1360170220790589E-4 76-77 0.0 0.0 0.0 0.5854463723284429 1.1360170220790589E-4 78-79 0.0 2.8400425551976472E-5 0.0 0.6761005306903518 1.1360170220790589E-4 80-81 0.0 5.6800851103952945E-5 0.0 0.777262846506492 1.4200212775988236E-4 82-83 0.0 5.6800851103952945E-5 0.0 0.8830544316876043 1.7040255331185881E-4 84-85 0.0 5.6800851103952945E-5 0.0 0.992112065807194 1.7040255331185881E-4 86-87 0.0 5.6800851103952945E-5 0.0 1.116704732703715 1.988029788638353E-4 88-89 5.6800851103952945E-5 5.6800851103952945E-5 0.0 1.2567188306749588 2.2720340441581178E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5175 0.0 54.89966 1 TTCGCGA 25 0.0016148251 38.006912 90-91 GTATCAA 8505 0.0 37.03548 1 TATCAAC 11660 0.0 26.97126 2 ATCAACG 11590 0.0 26.970205 3 TCAACGC 11805 0.0 26.479008 4 CAACGCA 12125 0.0 25.81936 5 AACGCAG 12455 0.0 25.245386 6 TAAGGTG 950 0.0 24.502718 5 GTAAGGT 850 0.0 23.47319 4 AGGTAAG 870 0.0 22.933578 2 AGTACTT 9930 0.0 21.955511 12-13 ACGCAGA 14300 0.0 21.922396 7 TGGTATC 2355 0.0 21.584135 2 CGCAGAG 14505 0.0 21.547071 8 GAGTACT 9905 0.0 21.387524 12-13 GGTAAGG 1005 0.0 21.271015 3 GTGGTAT 2565 0.0 20.930033 1 AGAGTAC 13935 0.0 20.570765 10-11 AAGGTAA 1040 0.0 20.556917 1 >>END_MODULE