FastQCFastQC Report
Thu 26 May 2016
SRR937538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697406
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108340.6382680395851081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83930.4944603707068315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59390.3498868273117922No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21640.12748865032879583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.11111071835494866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.10374654030915409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.048.761931
GTATCAA70650.043.547221
ATCAACG91250.033.2836573
TATCAAC92700.032.9197732
TCAACGC92450.032.909854
CAACGCA94600.032.0604485
AACGCAG97150.031.2936746
GTGGTAT19000.028.2808971
ACGCAGA111000.027.217837
CGCAGAG113500.026.5772518
TGGTATC19750.026.2337842
GTACATG67050.025.7440091
TACATGG67900.024.7119182
TACGCTA1351.3425999E-524.631099
GCAGAGT122150.024.0333569
ACATGGG68700.023.8685153
TATACGA1050.00218761522.635092
GAGTACT76450.021.74560412-13
AGAGTAC111500.021.49184410-11
AGTACTT80850.021.00278912-13
Produced by FastQC (version 0.11.5)