FastQCFastQC Report
Thu 26 May 2016
SRR937538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697406
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106600.628017103745362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95650.5635069040642016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72790.42883081596271017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23490.1383876338365718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22640.13337999276543147No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.12289340322822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.052.176471
GTATCAA84100.036.156111
ATCAACG111650.027.1887153
TGGTATC24200.026.6974682
TATCAAC113600.026.680192
TCAACGC114150.026.634874
CAACGCA117250.025.9306665
AACGCAG120400.025.2872336
GTGGTAT26300.025.110581
GGACCGA5350.023.9706486
TAAGGTG7600.023.127825
ACCGAGT5200.022.835968
GAGTACT93950.022.4222612-13
ACGCAGA136400.022.2171767
CGCAGAG136650.022.1070128
GTACATG66600.021.686881
AGTACTT95800.021.34470712-13
ATACCGT2457.530616E-921.3253616
AATACCG1805.16569E-621.1136845
GTACTTT99100.021.06468614-15
Produced by FastQC (version 0.11.5)