##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697406 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.320281064164966 31.0 31.0 34.0 30.0 34.0 2 31.588284712084203 31.0 31.0 34.0 30.0 34.0 3 31.635675259778743 31.0 31.0 34.0 30.0 34.0 4 35.47101695174873 37.0 35.0 37.0 33.0 37.0 5 35.315786559020054 37.0 35.0 37.0 33.0 37.0 6 35.398098628142 37.0 35.0 37.0 33.0 37.0 7 35.31395611892499 37.0 35.0 37.0 32.0 37.0 8 35.2758868532337 37.0 35.0 37.0 32.0 37.0 9 36.799804525257954 39.0 37.0 39.0 33.0 39.0 10-11 36.66467686575869 38.0 35.0 39.0 32.0 39.0 12-13 36.513966900081655 38.0 35.0 39.0 32.0 39.0 14-15 37.413644113429555 39.0 36.0 40.0 32.0 41.0 16-17 37.32074913132156 39.0 36.0 40.0 32.0 41.0 18-19 37.2697153774642 39.0 36.0 40.0 32.0 41.0 20-21 37.10427852853118 39.0 36.0 40.0 31.0 41.0 22-23 36.8700219629246 38.5 36.0 40.0 31.0 41.0 24-25 36.92422879381833 39.0 36.0 40.0 31.0 41.0 26-27 36.67755062725123 38.0 35.5 40.0 30.5 41.0 28-29 36.434549247498836 38.0 35.0 40.0 30.0 41.0 30-31 36.428499427950655 38.0 35.0 40.0 30.0 41.0 32-33 36.2734946736373 38.0 35.0 40.0 30.0 41.0 34-35 36.04679670037693 38.0 35.0 40.0 30.0 41.0 36-37 35.76187017130846 38.0 34.5 40.0 29.0 41.0 38-39 35.5183904145502 38.0 34.0 40.0 28.5 41.0 40-41 35.217152820244536 38.0 34.0 40.0 27.0 41.0 42-43 34.88759819395006 38.0 33.5 40.0 26.5 41.0 44-45 34.419374327650544 37.5 33.0 40.0 24.5 41.0 46-47 33.93249788206239 37.0 33.0 40.0 23.5 41.0 48-49 33.436123708765024 37.0 32.0 39.5 22.5 40.5 50-51 33.03567885349763 37.0 31.5 39.0 20.5 40.0 52-53 32.60955776048865 36.0 31.0 39.0 19.0 40.0 54-55 32.222457679541606 36.0 31.0 39.0 18.0 40.0 56-57 31.77908555760967 35.0 30.0 39.0 14.5 40.0 58-59 31.33470159761424 35.0 29.5 38.0 12.5 40.0 60-61 30.767331151180095 34.5 28.5 38.0 8.0 40.0 62-63 30.214264589614977 34.0 28.0 38.0 7.5 40.0 64-65 29.62621258555702 34.0 27.5 37.0 6.5 39.0 66-67 28.97539775398461 33.0 26.0 36.5 2.0 39.0 68-69 28.33234682804232 33.0 26.0 36.0 2.0 39.0 70-71 27.752833735712024 32.0 25.5 35.5 2.0 38.5 72-73 27.042216476199567 31.5 24.0 35.0 2.0 38.0 74-75 26.335686630069645 31.0 22.0 35.0 2.0 37.0 76-77 25.55850898370808 30.5 22.0 34.0 2.0 36.0 78-79 26.097274311508265 31.0 23.0 34.0 2.0 36.0 80-81 25.758303258030196 31.0 21.5 34.0 2.0 36.0 82-83 25.198566223991197 31.0 20.0 34.0 2.0 35.5 84-85 24.540460267019206 30.5 18.0 34.0 2.0 35.0 86-87 23.790519769577813 30.0 13.5 34.0 2.0 35.0 88-89 23.02421606851867 29.5 6.5 34.0 2.0 35.0 90-91 22.030683289678485 29.0 2.0 34.0 2.0 35.0 92-93 21.062175755240645 28.0 2.0 33.0 2.0 35.0 94-95 20.088149505775284 26.5 2.0 33.0 2.0 34.0 96-97 18.97971227861808 25.5 2.0 32.5 2.0 34.0 98-99 17.540309448652827 22.0 2.0 32.0 2.0 34.0 100-101 15.13268805459625 8.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2966573236071106 1101 2 0.31476498701043454 1101 3 0.21185560867090203 1101 4 0.19907452462312136 1101 5 0.20757900962806985 1101 6 0.22582881403787525 1101 7 0.17205083177410785 1101 8 0.14088589703716536 1101 9 0.31152447430054764 1101 10-11 0.3848807639808527 1101 12-13 0.3211865130253173 1101 14-15 0.40496139626144867 1101 16-17 0.3776505821834206 1101 18-19 0.35528362592521745 1101 20-21 0.3755573800909531 1101 22-23 0.32614121555843667 1101 24-25 0.33820235947385413 1101 26-27 0.18712765371993356 1101 28-29 0.22523741075416837 1101 30-31 0.36704750931506425 1101 32-33 0.6153169874092015 1101 34-35 0.5303338321945859 1101 36-37 0.4508771767968227 1101 38-39 0.4850263351944122 1101 40-41 0.4586830081543667 1101 42-43 0.5651336038012644 1101 44-45 0.7454716394409715 1101 46-47 0.6997531035902611 1101 48-49 0.6904713344809537 1101 50-51 0.6760344426963698 1101 52-53 0.5321951797755844 1101 54-55 0.6444750410846609 1101 56-57 0.738586533076603 1101 58-59 0.5629608211127213 1101 60-61 0.5789883923969761 1101 62-63 0.6163734040549294 1101 64-65 0.5983487988377156 1101 66-67 0.6036152172144718 1101 68-69 0.6396288347172892 1101 70-71 0.6982224541327327 1101 72-73 0.4031307347028843 1101 74-75 0.5188558989669652 1101 76-77 0.5014132397893398 1101 78-79 0.636212446402638 1101 80-81 0.6877177251624857 1101 82-83 0.5647477918786237 1101 84-85 0.7413703302497758 1101 86-87 0.7535946401854083 1101 88-89 0.9228084385104296 1101 90-91 0.9637605863757877 1101 92-93 0.9717996102797528 1101 94-95 0.8708611581402543 1101 96-97 1.074844839535249 1101 98-99 1.3546890233452622 1101 100-101 1.300615004584607 1102 1 0.25751234070262186 1102 2 0.25349293340183365 1102 3 0.1642571109685278 1102 4 0.11621326652969088 1102 5 0.0480527959723176 1102 6 0.13572138724274652 1102 7 0.14658948501263325 1102 8 0.11319358466317908 1102 9 0.15367443284795002 1102 10-11 0.25178398020401715 1102 12-13 0.1149449635445734 1102 14-15 0.3198896183187898 1102 16-17 0.2228809089371424 1102 18-19 0.31942489530851503 1102 20-21 0.3337337169062806 1102 22-23 0.38696850249313997 1102 24-25 0.1466937417272689 1102 26-27 0.22583062775115792 1102 28-29 0.13490061102394435 1102 30-31 0.09679759742203942 1102 32-33 0.2645598380012615 1102 34-35 0.036571311541983675 1102 36-37 0.16543844019665244 1102 38-39 0.1526210176222591 1102 40-41 0.18219247872701771 1102 42-43 0.3126727422225102 1102 44-45 0.42570827014655066 1102 46-47 0.27331534325150386 1102 48-49 0.40242305280946056 1102 50-51 0.3467108246825674 1102 52-53 0.2829947042166481 1102 54-55 0.4434673217154099 1102 56-57 0.418136091039198 1102 58-59 0.5038556380261419 1102 60-61 0.3969179193695034 1102 62-63 0.4876143553782306 1102 64-65 0.6907840946536759 1102 66-67 0.5540204522427992 1102 68-69 0.5549307863081907 1102 70-71 0.39491735370409486 1102 72-73 0.6499758583312811 1102 74-75 0.6264608099822482 1102 76-77 0.48851947578518207 1102 78-79 0.7180064356525406 1102 80-81 0.6368863243500194 1102 82-83 0.7619834105664687 1102 84-85 0.8723236372616476 1102 86-87 1.1449413845447118 1102 88-89 1.2229170578165913 1102 90-91 1.2305408997874672 1102 92-93 1.1395492050257054 1102 94-95 1.0999342537410293 1102 96-97 1.254363966594351 1102 98-99 1.091702777923448 1102 100-101 1.1388059976838285 1103 1 0.23797397654994867 1103 2 0.23042528645742166 1103 3 0.20488629933957725 1103 4 0.18399459245082284 1103 5 0.013329321290854068 1103 6 0.052178524845871266 1103 7 0.09799642404939846 1103 8 0.129146084336071 1103 9 0.25857688248140676 1103 10-11 0.20946087754715847 1103 12-13 0.22460734768473145 1103 14-15 0.2972021295909215 1103 16-17 0.37355794419814004 1103 18-19 0.1392316688438413 1103 20-21 0.0445906819352615 1103 22-23 0.2748324376788176 1103 24-25 0.20487916699170938 1103 26-27 0.19643113627908093 1103 28-29 0.1049901161817175 1103 30-31 0.1883202460108535 1103 32-33 0.3756031689652346 1103 34-35 0.3561075538813583 1103 36-37 0.3761309646947524 1103 38-39 0.23420650724836634 1103 40-41 0.3479432293184743 1103 42-43 0.4586446564871025 1103 44-45 0.589830529028994 1103 46-47 0.6015138883948481 1103 48-49 0.6308097320655577 1103 50-51 0.8334388824807561 1103 52-53 0.656407322640348 1103 54-55 0.7946733957596237 1103 56-57 0.660823791948399 1103 58-59 0.7772618910099389 1103 60-61 0.7474379404461828 1103 62-63 0.974066577447207 1103 64-65 0.9013882102642086 1103 66-67 1.1771811053369312 1103 68-69 0.9159431741615158 1103 70-71 0.9746480248690048 1103 72-73 0.9819300130185447 1103 74-75 1.1978669002936932 1103 76-77 1.022945896604412 1103 78-79 1.104020856910278 1103 80-81 1.131981399267712 1103 82-83 1.2247190644562842 1103 84-85 1.1227382749443429 1103 86-87 1.2391640405598743 1103 88-89 1.1893711895175443 1103 90-91 1.4825309838466367 1103 92-93 1.4799587857200365 1103 94-95 1.3527094087218074 1103 96-97 1.33399915616069 1103 98-99 1.1688642868156158 1103 100-101 0.9752354102598169 1104 1 0.26812630406355353 1104 2 0.2484694326325645 1104 3 0.1672761682633741 1104 4 0.1161996256319 1104 5 0.12454999017413115 1104 6 0.13780652335200472 1104 7 0.19042979351979028 1104 8 0.17442853548678272 1104 9 0.34231940369870273 1104 10-11 0.2620803604092714 1104 12-13 0.28982367482939964 1104 14-15 0.36229503886574577 1104 16-17 0.36174054140909107 1104 18-19 0.27679430388529624 1104 20-21 0.1160060637708753 1104 22-23 0.17295126962034857 1104 24-25 0.1393618552256939 1104 26-27 0.30669857694399383 1104 28-29 0.24830642427505012 1104 30-31 0.3263564238898411 1104 32-33 0.4755012405468406 1104 34-35 0.48409122341026034 1104 36-37 0.5124070676687253 1104 38-39 0.3999849621549103 1104 40-41 0.5850997382975507 1104 42-43 0.6576458600475874 1104 44-45 0.6245252200571798 1104 46-47 0.6625309839433342 1104 48-49 0.6548827372320929 1104 50-51 0.6964130112224183 1104 52-53 0.60100677967732 1104 54-55 0.4331118629739379 1104 56-57 0.5466680268785673 1104 58-59 0.7935006019603783 1104 60-61 0.6370287487821997 1104 62-63 0.8015689361838305 1104 64-65 0.8484886274279511 1104 66-67 0.6480212238180876 1104 68-69 0.6553119437326025 1104 70-71 0.8076292487206729 1104 72-73 0.6818803812687406 1104 74-75 0.6301145532751526 1104 76-77 0.7236888419406924 1104 78-79 0.9662506984062027 1104 80-81 0.9893156337593929 1104 82-83 1.1523792771054637 1104 84-85 1.2489373938957335 1104 86-87 1.388781558564137 1104 88-89 1.4434266856006808 1104 90-91 1.4842426744106305 1104 92-93 1.214802579899093 1104 94-95 1.476087966293882 1104 96-97 1.749298870693078 1104 98-99 1.6708486118011372 1104 100-101 1.461851564430468 1105 1 0.05424212659987049 1105 2 0.085171827607045 1105 3 0.09011009052485974 1105 4 -0.04576150511014987 1105 5 -0.15876253436454135 1105 6 -0.06747459168528991 1105 7 0.029414481387867397 1105 8 -0.0749440597232649 1105 9 0.030531810451741137 1105 10-11 0.09042193873671778 1105 12-13 0.05663188056163193 1105 14-15 0.1538488276368568 1105 16-17 0.05280512719629371 1105 18-19 0.046126263052947536 1105 20-21 0.14350724162717654 1105 22-23 0.0035244930204214597 1105 24-25 0.18178745396931362 1105 26-27 -0.07358312702145753 1105 28-29 -0.11601002645129199 1105 30-31 0.047779666920853 1105 32-33 0.04198200222725035 1105 34-35 -0.10944686061015574 1105 36-37 -0.04964966574313223 1105 38-39 0.04904091032923219 1105 40-41 0.11737241203135085 1105 42-43 0.12384020633734139 1105 44-45 -0.019860527881569112 1105 46-47 -0.019058925754343647 1105 48-49 0.08744359314101047 1105 50-51 0.2826735137744407 1105 52-53 0.06631518171894157 1105 54-55 0.11985724970342204 1105 56-57 -0.11737732058904271 1105 58-59 0.1818372725532349 1105 60-61 0.2265424904390514 1105 62-63 0.19450788396553875 1105 64-65 0.2770775555787175 1105 66-67 0.29786260583614776 1105 68-69 -0.07188338410406558 1105 70-71 0.07390918590166962 1105 72-73 -0.14801321904535314 1105 74-75 -0.013947676799443087 1105 76-77 0.07140517488140574 1105 78-79 0.21424422019654443 1105 80-81 -0.11073148049266379 1105 82-83 0.14602729438069062 1105 84-85 0.24739695416271346 1105 86-87 0.3468861914655932 1105 88-89 0.4439514205830122 1105 90-91 0.4990889641632812 1105 92-93 0.7687150259140161 1105 94-95 0.6893372792110419 1105 96-97 0.6743842652749379 1105 98-99 0.7441297268384339 1105 100-101 0.6414838769550073 1106 1 0.08234452727970876 1106 2 0.1672015705430958 1106 3 0.20096668708317722 1106 4 0.11056736678277446 1106 5 0.13657037406970574 1106 6 0.15857299701239214 1106 7 0.1170575661552391 1106 8 0.1752072043515156 1106 9 0.018780801316410134 1106 10-11 -0.07552579867564901 1106 12-13 -0.24081086428646614 1106 14-15 0.09045388393597875 1106 16-17 0.38028037682450133 1106 18-19 0.09608780968562058 1106 20-21 -0.08465783166791141 1106 22-23 -0.07732742964794426 1106 24-25 0.2111218495503593 1106 26-27 0.2578041684958592 1106 28-29 0.26054807849877903 1106 30-31 0.35584956282058045 1106 32-33 0.39410458561369666 1106 34-35 0.134349400277884 1106 36-37 0.0722965845224337 1106 38-39 0.1453978255618651 1106 40-41 0.23651071668399481 1106 42-43 0.23942720442529009 1106 44-45 0.2963606320929344 1106 46-47 0.46728736700112705 1106 48-49 0.5160562976236989 1106 50-51 0.6285290480850421 1106 52-53 0.6862072573509792 1106 54-55 0.7805396376362879 1106 56-57 0.5326845022280402 1106 58-59 0.3646318338449248 1106 60-61 0.4477982796891027 1106 62-63 0.5322563437063472 1106 64-65 0.7125256116653951 1106 66-67 0.7335379829340845 1106 68-69 0.7961002572811076 1106 70-71 0.946059175279192 1106 72-73 0.8275091943814559 1106 74-75 0.4896446325270922 1106 76-77 0.5054500017350279 1106 78-79 0.6708222954036813 1106 80-81 0.7909670536054882 1106 82-83 0.7937266782770145 1106 84-85 0.8478464973961941 1106 86-87 1.0754277253512932 1106 88-89 0.8661961624568164 1106 90-91 0.9506969947560151 1106 92-93 1.1472703690200277 1106 94-95 1.4701634954846732 1106 96-97 1.5451554571168735 1106 98-99 1.5614110100336767 1106 100-101 1.2407953279853885 1107 1 0.2279074820528102 1107 2 0.32084436623084045 1107 3 0.2588448262867864 1107 4 0.11656789458199768 1107 5 0.1808883407280959 1107 6 0.10247740649040793 1107 7 0.22896228367964255 1107 8 0.1405713612903554 1107 9 0.2875493123045416 1107 10-11 0.24029606221884592 1107 12-13 0.33929284629905254 1107 14-15 0.3740500704394307 1107 16-17 0.3092236894219269 1107 18-19 0.36988328944824644 1107 20-21 0.4541267045996449 1107 22-23 0.3783229651713853 1107 24-25 0.2803551525041783 1107 26-27 0.39147986824677616 1107 28-29 0.4081215509958369 1107 30-31 0.5897231137026822 1107 32-33 0.492221897330154 1107 34-35 0.7132520733160774 1107 36-37 0.4845096182201587 1107 38-39 0.6143037801770177 1107 40-41 0.7580366843077329 1107 42-43 0.7529966968452939 1107 44-45 0.8881073188774522 1107 46-47 0.7801609590868779 1107 48-49 0.8927705414335847 1107 50-51 0.9356064138371494 1107 52-53 0.7862237388137459 1107 54-55 0.9214316183255065 1107 56-57 0.8052387218258694 1107 58-59 0.8225123765951956 1107 60-61 0.8259118536452519 1107 62-63 1.1173894756649823 1107 64-65 1.1058331567417063 1107 66-67 1.1028544535073195 1107 68-69 1.2669007217051806 1107 70-71 1.2049724092070662 1107 72-73 1.3363885824482864 1107 74-75 1.3359121943595547 1107 76-77 1.129994308910252 1107 78-79 1.4975088003231036 1107 80-81 1.4488409498134764 1107 82-83 1.4557045077664732 1107 84-85 1.4316029857458652 1107 86-87 1.703967315688839 1107 88-89 1.7813254585512013 1107 90-91 1.9402936845327048 1107 92-93 2.1281261199223387 1107 94-95 2.3624381286494973 1107 96-97 2.1626893790084765 1107 98-99 2.310975060627843 1107 100-101 2.1119982147920773 1108 1 0.10443807624409729 1108 2 0.07154511715274836 1108 3 -0.031032766617997254 1108 4 -0.007042293287490509 1108 5 -0.13667605379530556 1108 6 -0.16467437274714314 1108 7 -0.11683839217535308 1108 8 -0.036995510188511105 1108 9 0.08158161888086113 1108 10-11 -0.06818561462945638 1108 12-13 -0.0033670247476109694 1108 14-15 -0.13052446190884837 1108 16-17 -0.12684128769150504 1108 18-19 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92-93 1.7372356339691777 2316 94-95 1.7752551604208797 2316 96-97 1.8351364984672252 2316 98-99 2.0547697519128754 2316 100-101 2.333910142430639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 42.0 5 106.0 6 238.0 7 558.0 8 1332.0 9 2193.0 10 3310.0 11 5184.0 12 7358.0 13 10109.0 14 13098.0 15 17393.0 16 22633.0 17 28484.0 18 26843.0 19 22428.0 20 22188.0 21 23565.0 22 25870.0 23 28751.0 24 32976.0 25 38536.0 26 44927.0 27 53872.0 28 63449.0 29 74581.0 30 86841.0 31 100916.0 32 118322.0 33 138122.0 34 161605.0 35 182919.0 36 178397.0 37 125839.0 38 33605.0 39 794.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.92969512773571 21.81756687880825 16.58269190996366 20.670046083492373 2 17.89507047812957 22.44548446276259 36.7153173725084 22.944127686599437 3 21.09901814886951 24.815100217626192 30.561221063198786 23.524660570305514 4 15.601747607820403 18.549716449688525 34.67797333107106 31.170562611420017 5 14.130578255263885 37.81216191053107 33.47477602125117 14.58248381295388 6 32.807295367166134 36.90484185869497 15.558446240911131 14.72941653322776 7 28.57825411245159 33.67585598259933 19.611454183619003 18.134435721330078 8 27.31827270552832 35.61563939328599 18.340161399217394 18.7259265019683 9 26.522823649733766 16.577766309297832 19.62011445700086 37.27929558396754 10-11 25.743347715086344 27.10731171779519 25.891014256758325 21.258326310360136 12-13 26.4640530326797 24.201108951886404 27.21498012953581 22.119857885898085 14-15 23.15038358530605 25.492516227702744 25.690789357407716 25.666310829583495 16-17 22.222997915643045 28.913206386686507 26.509273562129508 22.35452213554094 18-19 22.32444683240191 26.602592426325817 29.7218815062513 21.35107923502097 20-21 22.752835797681875 25.61099112410348 30.27163212572596 21.36454095248868 22-23 22.62232401263707 25.559808823854308 30.266983820724498 21.550883342784132 24-25 22.638190273864943 25.86841333187228 30.07200398726056 21.421392407002212 26-27 22.209847039004217 26.16399791798425 29.999790855803326 21.62636418720821 28-29 22.23130156656002 26.27553086916524 30.011167200773663 21.482000363501076 30-31 22.166205374553876 26.179417299102276 30.015977320688158 21.63840000565569 32-33 22.122314873400942 26.095554039516767 29.886014306535973 21.896116780546315 34-35 22.629854024317105 26.056906833132437 29.817998758105013 21.49524038444544 36-37 22.662609888264797 25.945295350670378 29.65327682357668 21.738817937488143 38-39 22.389758958598875 26.19135498264852 29.707721578609238 21.71116448014337 40-41 22.5732467340424 26.072988839083855 29.714772005244498 21.63899242162924 42-43 22.46180043572471 26.210594385662034 29.406674925501093 21.92093025311216 44-45 22.516710792821517 26.361931087789248 29.34256742346852 21.778790695920716 46-47 22.807919849464415 26.704335910206517 28.65351601207961 21.834228228249458 48-49 22.888778524407243 26.82758279398093 28.25473104254374 22.028907639068084 50-51 22.860941931394137 27.157085576461963 28.100112760294238 21.881859731849655 52-53 23.256022205654453 27.02989945537469 27.875395714223856 21.838682624747 54-55 23.048316077591334 27.153874794834 27.871764327450236 21.926044800124426 56-57 22.998622604138312 27.34746430730185 27.820097254280945 21.833815834278894 58-59 23.007606901354183 26.932006838670304 27.81382297458593 22.24656328538959 60-61 22.849777837476715 27.07540211357801 28.076046626440583 21.99877342250469 62-63 22.790569845988525 27.166835748194597 28.286308638004108 21.75628576781277 64-65 22.640428983990866 27.06102723803262 28.15021862771782 22.14832515025869 66-67 22.615950456166644 27.297888660697563 27.966556027255706 22.11960485588009 68-69 22.78020779360029 27.027071610172115 28.02762804762916 22.165092548598434 70-71 22.82297222939002 27.151577171283712 27.852764748092092 22.172685851234178 72-73 22.883358489365538 27.2491378020344 27.607684902728046 22.25981880587202 74-75 22.847480213926428 27.604857058358462 27.259211997601046 22.288450730114068 76-77 22.848481742140656 27.395802771994443 27.61681648350483 22.13889900236007 78-79 22.910223010876596 27.35971829957005 27.558020885987204 22.17203780356615 80-81 22.981721221004644 27.54592229140297 27.347825843618395 22.124530643973994 82-83 22.96450084645234 27.510569975995335 27.30133555951133 22.223593618040997 84-85 22.935519029103336 27.49522799575822 27.306439260044773 22.262813715093674 86-87 23.137069151399253 27.493275032608583 27.185157823172535 22.184497992819633 88-89 22.958679302417924 27.492273504394355 27.081735306697396 22.467311886490325 90-91 23.030721179708387 27.426699810809712 27.120878512260838 22.421700497221064 92-93 23.104402288956955 27.218097205848824 27.1500800946407 22.52742041055352 94-95 22.990286354590474 27.134816302051483 27.168102386818475 22.706794956539568 96-97 23.19374386563969 26.98706143374066 27.12692190318639 22.69227279743326 98-99 22.938934434559606 27.129509264422442 26.92033400878589 23.01122229223206 100-101 23.433046628393512 26.58993376343985 27.297818218029708 22.67920139013693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2014.0 1 1292.0 2 746.5 3 1024.0 4 911.0 5 818.5 6 1574.5 7 3241.0 8 4712.5 9 5069.0 10 4176.0 11 3126.5 12 2995.5 13 3149.5 14 3257.0 15 3313.0 16 3356.5 17 3447.0 18 3563.5 19 3747.5 20 3942.5 21 4350.5 22 4964.0 23 5748.0 24 6732.5 25 8061.0 26 9784.0 27 11593.0 28 13131.0 29 15225.5 30 17985.0 31 20868.0 32 24218.0 33 26363.5 34 27586.0 35 29477.5 36 33145.0 37 37467.0 38 40956.0 39 43023.5 40 44818.0 41 48228.0 42 51292.0 43 53877.5 44 57394.5 45 60139.5 46 60708.0 47 61811.0 48 63581.0 49 63688.0 50 62931.0 51 61622.5 52 60057.0 53 59925.5 54 60024.5 55 56605.5 56 51468.0 57 46839.5 58 42952.0 59 38862.5 60 32665.0 61 26999.5 62 22147.0 63 16764.0 64 12500.0 65 9785.0 66 7573.5 67 5744.0 68 4254.5 69 3180.0 70 2447.0 71 1810.5 72 1302.0 73 1050.5 74 869.5 75 701.0 76 514.0 77 347.5 78 259.0 79 180.5 80 118.0 81 85.5 82 56.0 83 34.5 84 21.0 85 11.5 86 5.0 87 3.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015847711154549943 2 0.0 3 0.0 4 0.0 5 0.021797967015551964 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.9456712183178334E-5 12-13 0.0022092534137383747 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010898983507775983 24-25 0.0 26-27 7.364178045794583E-4 28-29 0.0014433788969757382 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.9456712183178334E-5 40-41 5.596775314803883E-4 42-43 0.013756284589544282 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.9456712183178334E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.9456712183178334E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.9456712183178334E-5 82-83 0.0016201191700748083 84-85 3.5348054619814E-4 86-87 0.0 88-89 0.0 90-91 0.010192022415379703 92-93 0.00229762355028791 94-95 0.0 96-97 0.0 98-99 0.001502292321342095 100-101 0.018086421280471495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1697406.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.6319943352801 #Duplication Level Percentage of deduplicated Percentage of total 1 71.96183182126656 35.7160922930948 2 14.353279152972595 14.247637392260595 3 5.461506644326175 8.131964004998737 4 2.5451746459468114 5.052883744397226 5 1.4968794628544013 3.7146556510493376 6 0.9351636885917459 2.784842333684711 7 0.6211826056476035 2.158137209527347 8 0.4433133174008815 1.7602019246395821 9 0.3281000561081662 1.4655834113549027 >10 1.7137171510626543 15.010166652737333 >50 0.09564790380904588 3.1804522063117506 >100 0.04041157548956965 3.582397615885957 >500 0.0027254816889779395 0.9803037205478448 >1k 7.109952232116364E-4 0.5970748362732013 >5k 2.3699840773721214E-4 0.9906548997424944 >10k+ 1.1849920386860607E-4 0.6269521034941219 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10660 0.628017103745362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9565 0.5635069040642016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7279 0.42883081596271017 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2349 0.1383876338365718 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2264 0.13337999276543147 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.12289340322822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.891342436635667E-5 2.9456712183178334E-5 22-23 0.0 0.0 0.0 1.4728356091589165E-4 1.1782684873271333E-4 24-25 0.0 0.0 0.0 4.1239397056449666E-4 1.1782684873271333E-4 26-27 0.0 0.0 0.0 6.480476680299233E-4 1.1782684873271333E-4 28-29 0.0 0.0 0.0 0.001148811775143955 1.1782684873271333E-4 30-31 0.0 0.0 0.0 0.002562733959936515 1.4728356091589165E-4 32-33 0.0 0.0 0.0 0.0045363336762094635 1.7674027309907E-4 34-35 0.0 0.0 0.0 0.00724635119706187 1.7674027309907E-4 36-37 0.0 0.0 0.0 0.012931496648415287 1.7674027309907E-4 38-39 0.0 0.0 0.0 0.02182742372773514 1.7674027309907E-4 40-41 0.0 0.0 0.0 0.031577595460367175 1.7674027309907E-4 42-43 0.0 0.0 0.0 0.04123939705644966 1.7674027309907E-4 44-45 0.0 0.0 0.0 0.05446546082669673 1.7674027309907E-4 46-47 0.0 0.0 0.0 0.07028371526906349 1.7674027309907E-4 48-49 0.0 0.0 0.0 0.08589577272614801 2.0619698528224833E-4 50-51 0.0 0.0 0.0 0.10457132825028309 2.3565369746542667E-4 52-53 0.0 0.0 0.0 0.12433678212519575 2.3565369746542667E-4 54-55 0.0 0.0 0.0 0.1472541042037085 2.3565369746542667E-4 56-57 0.0 0.0 0.0 0.1769759267965354 2.3565369746542667E-4 58-59 0.0 0.0 0.0 0.2111457129290223 2.3565369746542667E-4 60-61 0.0 0.0 0.0 0.24964563575243637 2.3565369746542667E-4 62-63 0.0 0.0 0.0 0.28867577939514766 2.3565369746542667E-4 64-65 0.0 0.0 0.0 0.33159420904603853 2.945671218317833E-4 66-67 0.0 0.0 0.0 0.3859712997361857 2.945671218317833E-4 68-69 0.0 0.0 0.0 0.44856681312543967 2.945671218317833E-4 70-71 0.0 0.0 0.0 0.5180257404533741 2.945671218317833E-4 72-73 0.0 0.0 0.0 0.588692392980819 2.945671218317833E-4 74-75 0.0 0.0 0.0 0.6710239035328024 2.945671218317833E-4 76-77 0.0 0.0 0.0 0.7640482006072795 2.945671218317833E-4 78-79 0.0 2.9456712183178334E-5 0.0 0.8718008537733459 2.945671218317833E-4 80-81 0.0 5.891342436635667E-5 0.0 0.9893625920964106 2.945671218317833E-4 82-83 0.0 5.891342436635667E-5 0.0 1.11428850846527 2.945671218317833E-4 84-85 0.0 5.891342436635667E-5 0.0 1.2441336957687201 2.945671218317833E-4 86-87 0.0 5.891342436635667E-5 0.0 1.3843771024728322 2.945671218317833E-4 88-89 0.0 5.891342436635667E-5 0.0 1.5374047222644434 2.945671218317833E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5245 0.0 52.17647 1 GTATCAA 8410 0.0 36.15611 1 ATCAACG 11165 0.0 27.188715 3 TGGTATC 2420 0.0 26.697468 2 TATCAAC 11360 0.0 26.68019 2 TCAACGC 11415 0.0 26.63487 4 CAACGCA 11725 0.0 25.930666 5 AACGCAG 12040 0.0 25.287233 6 GTGGTAT 2630 0.0 25.11058 1 GGACCGA 535 0.0 23.970648 6 TAAGGTG 760 0.0 23.12782 5 ACCGAGT 520 0.0 22.83596 8 GAGTACT 9395 0.0 22.42226 12-13 ACGCAGA 13640 0.0 22.217176 7 CGCAGAG 13665 0.0 22.107012 8 GTACATG 6660 0.0 21.68688 1 AGTACTT 9580 0.0 21.344707 12-13 ATACCGT 245 7.530616E-9 21.325361 6 AATACCG 180 5.16569E-6 21.113684 5 GTACTTT 9910 0.0 21.064686 14-15 >>END_MODULE