FastQCFastQC Report
Thu 26 May 2016
SRR937537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605057
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106980.6665183853283715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80310.5003560621211582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59410.3701426179880216No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21500.13395162913217412No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.11482458255376599No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.11133560988799773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.052.4727751
GTATCAA66000.042.5640071
ATCAACG86350.032.138633
TATCAAC86700.032.0128862
TCAACGC88200.031.5144624
CAACGCA90500.030.7135435
AACGCAG92500.030.145616
ACGCAGA104200.026.5327937
CGCAGAG104950.026.2526598
GTACATG63850.025.6836741
TGGTATC18350.025.1227262
TACATGG64400.025.0913562
GTGGTAT19350.024.8107191
ACATGGG66250.024.1724623
GTATAGG3800.023.76671
GCAGAGT113900.023.6051569
TAAGGTG7200.023.0971365
GAGTACT71400.022.15198912-13
AGAGTAC104000.021.37367810-11
AGTACTT75000.021.21535312-13
Produced by FastQC (version 0.11.5)