##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937537_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1605057 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24480563618613 33.0 31.0 34.0 31.0 34.0 2 32.410582303307606 34.0 31.0 34.0 31.0 34.0 3 32.43732154060572 34.0 31.0 34.0 31.0 34.0 4 35.99726240251904 37.0 35.0 37.0 35.0 37.0 5 35.98702226774501 37.0 35.0 37.0 35.0 37.0 6 36.053242345910455 37.0 37.0 37.0 35.0 37.0 7 36.01220953523769 37.0 37.0 37.0 35.0 37.0 8 35.995373373032855 37.0 37.0 37.0 35.0 37.0 9 37.7114775363118 39.0 38.0 39.0 35.0 39.0 10-11 37.62111563639173 39.0 38.0 39.0 35.0 39.0 12-13 37.51705951875852 39.0 37.5 39.0 35.0 39.0 14-15 38.789757622314966 40.0 38.0 41.0 36.0 41.0 16-17 38.71921994047564 40.0 38.0 41.0 36.0 41.0 18-19 38.65343037661591 40.0 38.0 41.0 35.0 41.0 20-21 38.56609048775215 40.0 38.0 41.0 35.0 41.0 22-23 38.38070704031072 40.0 38.0 41.0 34.0 41.0 24-25 38.222293663091094 40.0 38.0 41.0 34.0 41.0 26-27 38.155600704523266 40.0 38.0 41.0 34.0 41.0 28-29 38.185185635151896 40.0 38.0 41.0 34.0 41.0 30-31 37.99154702917093 40.0 38.0 41.0 34.0 41.0 32-33 37.74505329094231 40.0 37.0 41.0 33.5 41.0 34-35 37.79675394705609 40.0 38.0 41.0 33.5 41.0 36-37 37.72376650798071 40.0 37.5 41.0 33.0 41.0 38-39 37.60261348973899 40.0 37.0 41.0 33.0 41.0 40-41 37.37610751518482 40.0 37.0 41.0 33.0 41.0 42-43 37.12855773969398 40.0 37.0 41.0 32.0 41.0 44-45 36.90166455147699 40.0 36.0 41.0 31.5 41.0 46-47 36.55569303769274 39.5 36.0 41.0 31.0 41.0 48-49 36.19201529914514 39.0 35.5 41.0 30.0 41.0 50-51 35.24046716097933 38.0 34.5 40.0 28.0 40.5 52-53 35.3302337549383 38.5 35.0 40.0 28.5 40.5 54-55 35.66365898531953 39.0 35.0 40.0 29.0 41.0 56-57 35.44385806859196 38.5 35.0 40.0 28.0 41.0 58-59 35.18729926725344 38.0 35.0 40.0 28.0 41.0 60-61 34.86107471572661 38.0 34.5 40.0 27.0 41.0 62-63 34.48778641506189 37.5 34.0 40.0 26.0 41.0 64-65 34.10151508638011 37.0 34.0 40.0 26.0 41.0 66-67 33.690128450266876 36.0 33.5 39.0 25.5 41.0 68-69 33.20410022821619 36.0 33.0 39.0 23.5 40.0 70-71 32.7645631899677 35.0 33.0 38.5 23.0 40.0 72-73 32.318457849160495 35.0 32.0 37.5 21.5 40.0 74-75 31.877218379160364 35.0 32.0 37.0 20.0 39.0 76-77 31.40601922548545 35.0 31.0 36.5 18.5 39.0 78-79 30.89922974698095 35.0 31.0 36.0 16.5 38.5 80-81 30.4128809755666 34.0 30.5 35.5 10.0 37.0 82-83 29.922651968123247 34.0 30.0 35.0 7.0 37.0 84-85 29.491522730968434 34.0 29.5 35.0 3.5 36.0 86-87 29.051617481497544 34.0 29.0 35.0 2.0 36.0 88-89 28.593734988850862 34.0 29.0 35.0 2.0 35.5 90-91 28.137200423411755 34.0 28.0 35.0 2.0 35.0 92-93 27.60145963663596 33.0 27.0 35.0 2.0 35.0 94-95 27.00463690697589 33.0 25.5 35.0 2.0 35.0 96-97 26.31327765929808 32.5 25.0 35.0 2.0 35.0 98-99 25.394071051682275 32.0 22.0 34.0 2.0 35.0 100-101 23.02131170419493 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.016035893118186095 1101 2 0.04347421920774508 1101 3 -0.036447271285091176 1101 4 -0.020177488003085386 1101 5 -0.10383691113477056 1101 6 -0.1380631752780488 1101 7 -0.1536910653903263 1101 8 -0.17388256249892464 1101 9 -0.03605123955277634 1101 10-11 -0.13131200443849167 1101 12-13 -0.00792436750349168 1101 14-15 0.04950833225722562 1101 16-17 0.10150942660417428 1101 18-19 0.015461777223848117 1101 20-21 0.17529898764117746 1101 22-23 0.07317633624381159 1101 24-25 0.12750053033650488 1101 26-27 0.07251344039582364 1101 28-29 0.08317158250213197 1101 30-31 0.07671630191850909 1101 32-33 -0.0174447732645433 1101 34-35 0.012806691883717747 1101 36-37 0.07749996123909142 1101 38-39 0.03314517189840416 1101 40-41 0.01633908807245632 1101 42-43 -0.1671809926327299 1101 44-45 0.029459362440228176 1101 46-47 0.18937365176176257 1101 48-49 0.187531352620681 1101 50-51 0.0559882465513013 1101 52-53 0.13892086544083782 1101 54-55 0.24191249443608598 1101 56-57 0.24466302011384045 1101 58-59 0.1430243867947425 1101 60-61 0.1515268550450486 1101 62-63 0.0750254924637801 1101 64-65 -0.24488123899299552 1101 66-67 -0.05837929787735874 1101 68-69 -0.11197542458536702 1101 70-71 -0.02520967784272443 1101 72-73 -0.08888768770069788 1101 74-75 -0.22229158741863486 1101 76-77 -0.08896273375885855 1101 78-79 0.15556784563458592 1101 80-81 0.0762228328435377 1101 82-83 0.0726143499494718 1101 84-85 0.4657556731424215 1101 86-87 0.3317419634646548 1101 88-89 0.2289590622397668 1101 90-91 0.48766946174295 1101 92-93 0.40921439584513664 1101 94-95 0.4495195488044281 1101 96-97 0.4109694909936472 1101 98-99 0.5903612837214531 1101 100-101 1.1669743961298948 1102 1 0.17135855841505787 1102 2 0.07616806591707359 1102 3 0.19614160730551333 1102 4 0.14425246438903372 1102 5 0.03841163997018526 1102 6 0.130326531890681 1102 7 0.08852882420703168 1102 8 0.15924157905169523 1102 9 0.16533456295082516 1102 10-11 0.12852950114677952 1102 12-13 0.18573492533894864 1102 14-15 0.2692436413734427 1102 16-17 0.23568957720736705 1102 18-19 0.30933454246377323 1102 20-21 0.34686435262873516 1102 22-23 0.30126703316511794 1102 24-25 0.40207181746153964 1102 26-27 0.2847079813091966 1102 28-29 0.18242117212145814 1102 30-31 0.20132499789436054 1102 32-33 0.21310541601307875 1102 34-35 0.2958160012902695 1102 36-37 0.3860935745618477 1102 38-39 0.3787688946932306 1102 40-41 0.3033712447950663 1102 42-43 0.3232205662554932 1102 44-45 0.4498365776583739 1102 46-47 0.4578696298450211 1102 48-49 0.540064545996529 1102 50-51 0.44255716631024455 1102 52-53 0.3290065894044645 1102 54-55 0.3850893806052298 1102 56-57 0.4961496962350793 1102 58-59 0.42703107944288377 1102 60-61 0.4014563179581785 1102 62-63 0.4485945573913952 1102 64-65 0.3390878586906254 1102 66-67 0.5097904229148895 1102 68-69 0.37351552470610017 1102 70-71 0.4696375786554299 1102 72-73 0.4480140033282467 1102 74-75 0.5386135578803248 1102 76-77 0.521255838153607 1102 78-79 0.5726404262497304 1102 80-81 0.6319517843162572 1102 82-83 0.4158453952011527 1102 84-85 0.4865397328774712 1102 86-87 0.5509327039365637 1102 88-89 0.750325657811004 1102 90-91 0.82367203755636 1102 92-93 0.9636600934550614 1102 94-95 1.036554869459696 1102 96-97 1.103045514711976 1102 98-99 1.20757121623663 1102 100-101 2.034321680545105 1103 1 0.10302497765328411 1103 2 0.07904376213925701 1103 3 0.08471704497124932 1103 4 0.04651308114146957 1103 5 0.0800926562303772 1103 6 0.018715833034704588 1103 7 0.0959995303125396 1103 8 0.07546808685172124 1103 9 -0.012164696647829487 1103 10-11 0.04279630034465498 1103 12-13 0.06178853717874233 1103 14-15 0.134496248226732 1103 16-17 0.13593961808958 1103 18-19 0.07518610197885778 1103 20-21 0.002870495887535185 1103 22-23 0.06755925393961348 1103 24-25 0.10399377124692677 1103 26-27 0.031984133302103146 1103 28-29 -0.05957401545818897 1103 30-31 -0.031070594211939806 1103 32-33 0.003828432057744635 1103 34-35 0.03373596710746796 1103 36-37 -0.03649228373475211 1103 38-39 0.040272094056000185 1103 40-41 0.001334971534589613 1103 42-43 0.10492481566939205 1103 44-45 -0.020341450244018233 1103 46-47 -0.00991989722503206 1103 48-49 0.2159115613689977 1103 50-51 0.18119473740195957 1103 52-53 0.12884464309467347 1103 54-55 0.15293286465891498 1103 56-57 0.15634070847864479 1103 58-59 0.14941166005318252 1103 60-61 0.20429290290311997 1103 62-63 0.31911494084770453 1103 64-65 0.3316212973254906 1103 66-67 0.3195852733387454 1103 68-69 0.2678708028068755 1103 70-71 0.20889384644485176 1103 72-73 0.42038780163613154 1103 74-75 0.3992511847376363 1103 76-77 0.4028121580160331 1103 78-79 0.6480332534374149 1103 80-81 0.5374543416210571 1103 82-83 0.574368526243525 1103 84-85 0.662577971498461 1103 86-87 0.7320088744677626 1103 88-89 0.676604668167581 1103 90-91 0.7863335788119734 1103 92-93 0.720730078526465 1103 94-95 0.6679563267504065 1103 96-97 0.9331669254610198 1103 98-99 0.9240146446754274 1103 100-101 1.3302469109361539 1104 1 0.11156124004540402 1104 2 0.189171512568592 1104 3 0.22818031296866081 1104 4 0.1396679624965742 1104 5 0.1572384620901346 1104 6 0.14007737575970225 1104 7 0.179880970050867 1104 8 0.08459136233013709 1104 9 0.2330388603784428 1104 10-11 0.2467294245375129 1104 12-13 0.28722067437053056 1104 14-15 0.38717876151643793 1104 16-17 0.42473230964014874 1104 18-19 0.35709708895262793 1104 20-21 0.381147189283773 1104 22-23 0.2956547923671238 1104 24-25 0.3404754059282027 1104 26-27 0.4045237976398326 1104 28-29 0.2913703930373899 1104 30-31 0.4672673396658098 1104 32-33 0.4352598429914778 1104 34-35 0.48213751682201433 1104 36-37 0.45470593283757665 1104 38-39 0.6153517503666635 1104 40-41 0.6029373807455158 1104 42-43 0.6612023063113099 1104 44-45 0.6633813594463405 1104 46-47 0.6872496075816485 1104 48-49 0.6851807437280257 1104 50-51 0.6866614674114402 1104 52-53 0.7301596889226545 1104 54-55 0.7737367449519255 1104 56-57 0.8819612359167337 1104 58-59 0.7390615272244077 1104 60-61 0.8496475816704532 1104 62-63 0.8161368732947807 1104 64-65 0.7052421578593666 1104 66-67 0.7345263945610441 1104 68-69 0.8555125391642733 1104 70-71 0.897244503489965 1104 72-73 0.9015439989926648 1104 74-75 0.8388727933719125 1104 76-77 0.8386154512959649 1104 78-79 0.9880394239005561 1104 80-81 1.1016203325401008 1104 82-83 1.1369809802039512 1104 84-85 1.1289550905474464 1104 86-87 1.2506540482645896 1104 88-89 1.2814452053801162 1104 90-91 1.303976052348606 1104 92-93 1.2817009435653297 1104 94-95 1.4701308689484556 1104 96-97 1.6117806735805296 1104 98-99 1.6224120989498374 1104 100-101 2.045288916500489 1105 1 0.11284236533449388 1105 2 -0.0187924758458351 1105 3 0.0322206727926897 1105 4 0.06596969814410159 1105 5 0.015388126123049517 1105 6 0.06992774090959131 1105 7 0.042427024170386574 1105 8 0.08132288778848817 1105 9 0.03639926076657218 1105 10-11 0.14326396603911462 1105 12-13 0.03775727326011946 1105 14-15 0.08113135404418159 1105 16-17 0.13760338728830135 1105 18-19 0.10741408720895151 1105 20-21 0.2050946018630242 1105 22-23 0.1699615182748886 1105 24-25 0.13498757880716283 1105 26-27 0.07653018867486594 1105 28-29 0.06131720736907198 1105 30-31 0.1866410765973825 1105 32-33 0.05956182669287813 1105 34-35 0.06860471899322107 1105 36-37 -0.05619530524306526 1105 38-39 0.0037647162884368868 1105 40-41 0.14192634234822776 1105 42-43 0.12352584151671664 1105 44-45 0.12212489363034962 1105 46-47 0.19315620800333733 1105 48-49 0.17056307499666445 1105 50-51 0.008919195383889189 1105 52-53 0.0672758437958123 1105 54-55 0.06711297701361474 1105 56-57 0.050893947656248884 1105 58-59 0.1374499066301027 1105 60-61 0.07275671807819606 1105 62-63 0.0634684702510171 1105 64-65 0.04797235549994383 1105 66-67 0.017836016862538884 1105 68-69 -0.00907995939481765 1105 70-71 0.1441821325326984 1105 72-73 0.10814587982466861 1105 74-75 0.14626990573302123 1105 76-77 0.29988475754765176 1105 78-79 0.34914530642516084 1105 80-81 0.24118273632803167 1105 82-83 0.2829862185180616 1105 84-85 0.4530170456496947 1105 86-87 0.6326467963367008 1105 88-89 0.6385822259612901 1105 90-91 0.6026329135916448 1105 92-93 0.6623724399867896 1105 94-95 0.5489127785255263 1105 96-97 0.7176865444320129 1105 98-99 1.003994098993612 1105 100-101 1.2685782567501462 1106 1 -0.022035842501708203 1106 2 0.019233311254055252 1106 3 0.02181915580796101 1106 4 -0.06297701188842098 1106 5 0.0644180679783517 1106 6 -0.023383225922088968 1106 7 -0.15175249024684945 1106 8 -0.1442383596498189 1106 9 0.013943074364476615 1106 10-11 -0.1153178118243332 1106 12-13 -0.09589337456181113 1106 14-15 -0.12455706527577348 1106 16-17 -0.22965333940972243 1106 18-19 0.01866569531394191 1106 20-21 0.04413508441072622 1106 22-23 -0.04927914172830583 1106 24-25 -0.07856083386846535 1106 26-27 -0.11856244227374901 1106 28-29 -0.021803739898757613 1106 30-31 0.16094803632537946 1106 32-33 0.16501783126461333 1106 34-35 0.09110670477216587 1106 36-37 -0.04997670686805833 1106 38-39 -0.04366470823897117 1106 40-41 -0.06873342843613273 1106 42-43 0.05243030675089244 1106 44-45 0.08108482396061589 1106 46-47 0.14362343000453848 1106 48-49 0.014357532861922095 1106 50-51 0.059085645357981775 1106 52-53 0.10534606312698003 1106 54-55 0.18895080347089532 1106 56-57 0.11672843280972245 1106 58-59 0.2383818897704586 1106 60-61 0.11735615125964216 1106 62-63 0.4107405363224217 1106 64-65 0.4075254902963863 1106 66-67 0.28261697963380783 1106 68-69 0.1600103829521018 1106 70-71 0.244135512868489 1106 72-73 0.37871301282469005 1106 74-75 0.5120879851395728 1106 76-77 0.4878968048344774 1106 78-79 0.630970609828946 1106 80-81 0.5709707877491113 1106 82-83 0.3660075008732697 1106 84-85 0.275949682285642 1106 86-87 0.46150026704082237 1106 88-89 0.46187920689371254 1106 90-91 0.6426034652759007 1106 92-93 0.7064088826193071 1106 94-95 0.8331242978207349 1106 96-97 0.8199379597694723 1106 98-99 1.0722537636874883 1106 100-101 1.6156017757555219 1107 1 0.0609296799909842 1107 2 0.00464428352623969 1107 3 -0.0055419447275895095 1107 4 0.013324592125684376 1107 5 0.020042707497474055 1107 6 0.0015605193681338392 1107 7 0.04100314594555954 1107 8 0.11248529253459338 1107 9 0.10300974943324803 1107 10-11 0.14252218236421044 1107 12-13 0.10223873276456175 1107 14-15 0.20853076909075696 1107 16-17 0.25247030979893736 1107 18-19 0.2212854887447122 1107 20-21 0.17125847542975237 1107 22-23 0.3587510813387951 1107 24-25 0.2580690123292797 1107 26-27 0.27533605506251746 1107 28-29 0.23412565092247206 1107 30-31 0.19885815307085863 1107 32-33 0.2068099824744678 1107 34-35 0.21803492192453433 1107 36-37 0.2860606159766519 1107 38-39 0.20156031497621285 1107 40-41 0.2023626347406804 1107 42-43 0.3227373598793193 1107 44-45 0.4109124670567539 1107 46-47 0.3610475550698524 1107 48-49 0.21037642359169695 1107 50-51 0.09405590103575179 1107 52-53 0.08444583023116792 1107 54-55 0.11063138174567655 1107 56-57 0.10053118992503585 1107 58-59 0.1418447817920523 1107 60-61 0.2804685530272266 1107 62-63 0.2697628640377303 1107 64-65 0.14926714136228014 1107 66-67 0.184127695379253 1107 68-69 0.18534928968087883 1107 70-71 0.3819133840029778 1107 72-73 0.4223566901103908 1107 74-75 0.531865749497328 1107 76-77 0.6900435423238775 1107 78-79 0.6205152420662401 1107 80-81 0.5908470142593245 1107 82-83 0.4818996423681945 1107 84-85 0.7134065230421491 1107 86-87 0.7477109270348166 1107 88-89 0.7477340903101464 1107 90-91 0.8463190330611887 1107 92-93 0.6901372461083142 1107 94-95 0.9913035269452273 1107 96-97 1.129061419055592 1107 98-99 1.4790805604467288 1107 100-101 2.3186959379563845 1108 1 -0.02834386505467279 1108 2 -0.07715572556012518 1108 3 0.02596502905824849 1108 4 -0.046554559207741875 1108 5 0.005731681192443716 1108 6 0.08579113133487937 1108 7 0.07541775682194185 1108 8 0.012398972058470292 1108 9 0.09914804392236931 1108 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0.45466341253428055 2316 90-91 0.3808335381037473 2316 92-93 0.5681101449048214 2316 94-95 0.3218831142007801 2316 96-97 0.5799230783898572 2316 98-99 0.7171869171044634 2316 100-101 1.0483987095214218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5852.0 3 881.0 4 855.0 5 941.0 6 1031.0 7 1092.0 8 1355.0 9 1680.0 10 2043.0 11 2652.0 12 3730.0 13 5425.0 14 5985.0 15 8021.0 16 10646.0 17 14953.0 18 13263.0 19 8661.0 20 8237.0 21 8308.0 22 8963.0 23 9895.0 24 11323.0 25 12214.0 26 14638.0 27 17291.0 28 20824.0 29 25935.0 30 32861.0 31 42471.0 32 57078.0 33 78861.0 34 115270.0 35 178162.0 36 262264.0 37 345280.0 38 240268.0 39 25841.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13033166027581 18.287645512579797 14.942988372739638 23.639034454404754 2 15.213628012192142 19.219055627433697 40.617653618441395 24.949662741932762 3 18.90575202609594 23.47697677876044 29.728546245710124 27.88872494943349 4 12.782711885578665 15.855474361376555 36.04946599213041 35.31234776091437 5 14.032731923993586 36.54192544256299 33.86056580111021 15.564776832333205 6 32.88821048754374 35.238683859770696 16.737243297405115 15.13586235528045 7 28.989531377775556 32.638567228280884 19.86566204515803 18.506239348785527 8 26.997979061402944 34.32442775971746 19.15814827833138 19.51944490054822 9 26.366245511819574 16.531363101990564 19.994418454811026 37.10797293137883 10-11 25.154055066491853 26.17306611725042 26.515483548729474 22.15739526752825 12-13 25.958131703269636 23.479041815229344 27.528583032045834 23.034243449455186 14-15 22.939299916968928 24.761800712943483 25.888213957960062 26.41068541212753 16-17 22.25597555775722 28.14847139217526 26.598606457541656 22.996946592525862 18-19 22.249084388579462 26.15672320831865 29.499247252385363 22.094945150716523 20-21 22.644546899324972 25.22824508772127 29.929569552173962 22.197638460779796 22-23 22.377803736348994 24.98612000121089 30.04446280893725 22.591613453502863 24-25 22.23409892835007 25.380264389002242 29.944466949502186 22.441169733145497 26-27 21.882117344558054 25.701672440393388 29.740711343523685 22.67549887152488 28-29 22.011924052916847 25.874988801104813 29.751102018111148 22.36198512786719 30-31 21.893719233734654 25.720925586607525 29.692719730052847 22.692635449604975 32-33 21.736208315279892 25.751439384763685 29.620421969800077 22.89193033015634 34-35 22.33093261090036 25.70240094093586 29.566874213194517 22.399792234969265 36-37 22.261123804928502 25.560493330882306 29.447079785661916 22.731303078527272 38-39 22.034971091500665 25.767998564328238 29.48498396573114 22.712046378439954 40-41 22.253361741729726 25.66455393002409 29.537606223298855 22.544478104947324 42-43 22.021879362927184 25.73476648809975 29.344558609494044 22.898795539479018 44-45 21.969742840360272 26.00198127217149 29.315992936333995 22.712282951134238 46-47 22.327188105882513 26.229586479917007 28.75539481224118 22.6878306019593 48-49 22.35824862940068 26.32234273535541 28.382580434921035 22.936828200322875 50-51 22.35306053326965 26.702908899637734 28.32831529806193 22.615715269030684 52-53 22.652136028205433 26.367945012770555 28.233123774028112 22.7467951849959 54-55 22.384152924787696 26.54693484646687 28.22935846538345 22.839553763361987 56-57 22.327805798061114 26.826930968166927 28.186092404629186 22.659170829142774 58-59 22.38672764237156 26.341566925829014 28.196894732653988 23.074810699145434 60-61 22.1290261977676 26.519953925233676 28.487961859430772 22.863058017567955 62-63 22.1913835607845 26.598444320784637 28.6308518596774 22.579320258753466 64-65 22.07592972675921 26.583861320913986 28.474380886906793 22.86582806542001 66-67 22.01894386590057 26.707380592820478 28.367613735129787 22.906061806149157 68-69 22.22515588187838 26.531830675406475 28.48031124256403 22.762702200151114 70-71 22.18031738106012 26.56931983085493 28.320751478458973 22.929611309625976 72-73 22.175816439069237 26.64746771724117 28.189281918097382 22.987433925592214 74-75 22.085701936025494 27.057075847820627 27.986780037107025 22.870442179046858 76-77 22.336592351399638 26.82541010316409 27.97874917130383 22.85924837413244 78-79 22.300480108176508 26.839829407242295 28.039428739629418 22.82026174495178 80-81 22.32233147645527 27.062707712744043 27.855699100858196 22.759261709942496 82-83 22.269427267072388 26.9826144846315 27.863110641141404 22.884847607154708 84-85 22.22084902368705 27.037249258628837 27.833423527509847 22.908478190174264 86-87 22.401506793522785 27.046912524518945 27.782894398055024 22.768686283903243 88-89 22.33546265151768 27.01367884322061 27.496356896490916 23.15450160877079 90-91 22.25803541917202 26.94496922724956 27.711767678296713 23.085227675281704 92-93 22.34080702653458 26.826271354393754 27.75577286855553 23.077148750516137 94-95 22.259802720589498 26.65665860924414 27.823544007103436 23.259994663062933 96-97 22.457358951249898 26.554163868451862 27.70766290603241 23.280814274265826 98-99 22.250760948910234 26.73999281573099 27.57769980428953 23.431546431069247 100-101 22.548542015342083 26.186957950486033 28.01226692514548 23.252233109026403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3020.0 1 1866.5 2 936.5 3 1135.0 4 837.0 5 724.5 6 1474.5 7 3226.5 8 5205.5 9 5679.5 10 4264.5 11 2925.0 12 2733.5 13 2778.5 14 2770.5 15 2728.0 16 2594.5 17 2606.5 18 2726.5 19 2832.0 20 3010.5 21 3478.5 22 4098.5 23 4743.0 24 5719.0 25 6951.5 26 8320.5 27 10067.5 28 11681.0 29 13547.0 30 16038.5 31 18606.5 32 21822.0 33 23787.0 34 25148.5 35 27161.5 36 30278.5 37 34385.0 38 37534.0 39 39743.5 40 41976.5 41 45383.0 42 48229.5 43 50725.0 44 54097.0 45 57038.0 46 57966.0 47 59085.0 48 61225.5 49 60947.0 50 59794.5 51 58804.0 52 57491.5 53 57955.0 54 58375.0 55 54812.0 56 50037.0 57 45670.0 58 42411.5 59 38469.0 60 31798.5 61 26391.0 62 21724.0 63 16326.0 64 12002.0 65 9499.0 66 7332.5 67 5388.0 68 3934.0 69 2926.0 70 2264.5 71 1682.5 72 1296.0 73 1079.5 74 881.0 75 706.0 76 521.0 77 340.0 78 225.0 79 162.5 80 117.0 81 77.0 82 57.0 83 36.5 84 25.0 85 17.5 86 15.5 87 14.5 88 8.0 89 5.0 90 6.5 91 6.5 92 3.0 93 3.5 94 5.0 95 3.0 96 3.5 97 3.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2862203647596316 2 0.27126762476348193 3 0.2613614345160328 4 0.2465333006865177 5 0.24478881435363356 6 0.20260962694782803 7 0.19482174153316675 8 0.20722005511330752 9 0.20871532911292248 10-11 0.20915145069614352 12-13 0.20684623661340376 14-15 0.20229811153124155 16-17 0.20186198994802054 18-19 0.2030145969893904 20-21 0.19008670720105267 22-23 0.18264148874463648 24-25 0.18366948961937177 26-27 0.1941675591583352 28-29 0.20787423748813905 30-31 0.2032326577810009 32-33 0.20753157052989393 34-35 0.20232926307290022 36-37 0.18743882616006785 38-39 0.18812416007655805 40-41 0.1837006411610304 42-43 0.195413620824681 44-45 0.1903982226176391 46-47 0.19033591953432183 48-49 0.19955677586528078 50-51 0.20774963132150448 52-53 0.21940030790183776 54-55 0.19759422874078614 56-57 0.1710531152476205 58-59 0.17317142008040834 60-61 0.1793394253288201 62-63 0.1863796737436739 64-65 0.1760373619130037 66-67 0.18083469932843504 68-69 0.1845728843274725 70-71 0.1795574861204306 72-73 0.1798066984536998 74-75 0.18282839799458836 76-77 0.19581859086624337 78-79 0.20127011065650627 80-81 0.19220501203384055 82-83 0.1908031926592015 84-85 0.18429252045254468 86-87 0.1856320367438664 88-89 0.19049167724261507 90-91 0.19136392040905711 92-93 0.18787494774328886 94-95 0.18781264465997158 96-97 0.17725227203769087 98-99 0.18351373191107856 100-101 0.17834257599574346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1605057.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.187975507833585 #Duplication Level Percentage of deduplicated Percentage of total 1 70.80672385325919 34.120326748307896 2 14.657363052509337 14.126173035674897 3 5.650713274864577 8.168892986728931 4 2.7929645449199647 5.383492283394033 5 1.5657164592304966 3.7724353194805555 6 1.0006264724101959 2.893089836699546 7 0.7087908676297551 2.390863787866313 8 0.47860690784440163 1.8450478362468794 9 0.3364578184307646 1.4591899002564723 >10 1.8577555465362598 15.841668799775915 >50 0.09650775096888606 3.1719167586285755 >100 0.04414851970313863 3.802538220460652 >500 0.002071389538886899 0.6869840905363219 >1k 0.0011651566156238805 0.7983413918083284 >5k 2.5892369236086237E-4 0.8716438170153915 >10k+ 1.2946184618043118E-4 0.6673951871192856 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10698 0.6665183853283715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8031 0.5003560621211582 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5941 0.3701426179880216 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2150 0.13395162913217412 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1843 0.11482458255376599 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1787 0.11133560988799773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.115154165864514E-5 0.0 12-13 0.0 0.0 0.0 6.230308331729029E-5 1.557577082932257E-4 14-15 0.0 0.0 0.0 6.230308331729029E-5 1.8690924995187086E-4 16-17 0.0 0.0 0.0 1.2460616663458057E-4 1.8690924995187086E-4 18-19 0.0 0.0 0.0 1.2460616663458057E-4 3.738184999037417E-4 20-21 0.0 0.0 0.0 1.2460616663458057E-4 4.3612158322103205E-4 22-23 0.0 0.0 0.0 2.18060791610516E-4 4.3612158322103205E-4 24-25 0.0 0.0 0.0 2.4921233326916114E-4 4.3612158322103205E-4 26-27 0.0 0.0 0.0 4.36121583221032E-4 4.6727312487967717E-4 28-29 0.0 0.0 0.0 8.722431664420641E-4 4.984246665383223E-4 30-31 0.0 0.0 0.0 0.0020248502078119342 4.984246665383223E-4 32-33 0.0 0.0 0.0 0.003893942707330643 4.984246665383223E-4 34-35 0.0 0.0 0.0 0.006635278373291416 4.984246665383223E-4 36-37 0.0 0.0 0.0 0.012273707413506186 4.984246665383223E-4 38-39 0.0 0.0 0.0 0.02096498753626818 5.607277498556126E-4 40-41 0.0 0.0 0.0 0.03133845090859702 5.607277498556126E-4 42-43 0.0 0.0 0.0 0.041338095781022106 5.607277498556126E-4 44-45 0.0 0.0 0.0 0.05460865252760494 5.607277498556126E-4 46-47 0.0 0.0 0.0 0.07236503127303268 5.607277498556126E-4 48-49 0.0 0.0 0.0 0.09264468489281066 5.607277498556126E-4 50-51 0.0 0.0 0.0 0.11469997638713142 5.607277498556126E-4 52-53 0.0 0.0 0.0 0.13837514804770173 5.607277498556126E-4 54-55 0.0 0.0 0.0 0.1661623232072132 5.607277498556126E-4 56-57 0.0 0.0 0.0 0.20257847540616936 5.918792915142577E-4 58-59 0.0 0.0 0.0 0.24055220468805782 6.230308331729029E-4 60-61 0.0 0.0 0.0 0.2813918758025416 6.230308331729029E-4 62-63 0.0 0.0 0.0 0.32687312662416346 6.853339164901932E-4 64-65 0.0 0.0 0.0 0.3770271086945822 6.853339164901932E-4 66-67 0.0 0.0 0.0 0.4358412193461042 6.853339164901932E-4 68-69 0.0 0.0 0.0 0.505122247994931 6.853339164901932E-4 70-71 0.0 0.0 0.0 0.5827518898082747 7.164854581488383E-4 72-73 0.0 0.0 0.0 0.6652100205787084 7.787885414661285E-4 74-75 0.0 0.0 0.0 0.7519982156396938 8.099400831247738E-4 76-77 0.0 0.0 0.0 0.852493089030483 8.099400831247738E-4 78-79 0.0 0.0 0.0 0.9723019182496322 8.099400831247738E-4 80-81 0.0 0.0 0.0 1.1024530592994517 8.099400831247738E-4 82-83 0.0 0.0 0.0 1.2415446928053022 8.099400831247738E-4 84-85 0.0 0.0 3.115154165864514E-5 1.3866797253929302 8.099400831247738E-4 86-87 0.0 0.0 6.230308331729029E-5 1.548480832767933 9.033947081007092E-4 88-89 0.0 0.0 6.230308331729029E-5 1.7282252281383155 9.968493330766446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3705 0.0 52.472775 1 GTATCAA 6600 0.0 42.564007 1 ATCAACG 8635 0.0 32.13863 3 TATCAAC 8670 0.0 32.012886 2 TCAACGC 8820 0.0 31.514462 4 CAACGCA 9050 0.0 30.713543 5 AACGCAG 9250 0.0 30.14561 6 ACGCAGA 10420 0.0 26.532793 7 CGCAGAG 10495 0.0 26.252659 8 GTACATG 6385 0.0 25.683674 1 TGGTATC 1835 0.0 25.122726 2 TACATGG 6440 0.0 25.091356 2 GTGGTAT 1935 0.0 24.810719 1 ACATGGG 6625 0.0 24.172462 3 GTATAGG 380 0.0 23.7667 1 GCAGAGT 11390 0.0 23.605156 9 TAAGGTG 720 0.0 23.097136 5 GAGTACT 7140 0.0 22.151989 12-13 AGAGTAC 10400 0.0 21.373678 10-11 AGTACTT 7500 0.0 21.215353 12-13 >>END_MODULE