FastQCFastQC Report
Thu 26 May 2016
SRR937537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605057
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101590.6329370234203521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93270.5811008581003666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65130.4057799816455116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22670.1412410898802971No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.1354469031317891No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.11469997638713142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.053.072451
GTATCAA76700.040.3285181
ACCGTAT1107.014023E-834.5463528
GTAAGGT6900.030.9761684
ATCAACG102600.029.8590933
TAAGGTG8300.029.7570275
TATCAAC103200.029.6854952
TCAACGC104600.029.378994
TACCGTA1303.0374576E-729.2315277
CAACGCA107050.028.617875
AACGCAG109900.027.8757326
GGTAAGG7750.026.965923
AGGTAAG7550.025.7929612
ACGCAGA126300.024.145527
CGCAGAG127900.023.8434648
TGGTATC20350.023.1065462
GAGTACT88450.022.47514312-13
GCAGAGT136600.022.220569
AGTACTT91500.021.72597312-13
GTGGTAT22450.021.3762571
Produced by FastQC (version 0.11.5)