##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937537_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1605057 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.396978425065278 31.0 31.0 34.0 30.0 34.0 2 31.67059425303899 31.0 31.0 34.0 30.0 34.0 3 31.69001786229399 31.0 31.0 34.0 30.0 34.0 4 35.496632206831286 37.0 35.0 37.0 33.0 37.0 5 35.351036754457944 37.0 35.0 37.0 33.0 37.0 6 35.43772090336979 37.0 35.0 37.0 33.0 37.0 7 35.371786796356766 37.0 35.0 37.0 33.0 37.0 8 35.31763295633738 37.0 35.0 37.0 32.0 37.0 9 36.86300984949444 39.0 37.0 39.0 33.0 39.0 10-11 36.71891029415155 38.0 36.0 39.0 32.0 39.0 12-13 36.563663782656946 38.0 35.0 39.0 32.0 39.0 14-15 37.47880199893213 39.0 36.0 40.0 32.0 41.0 16-17 37.3858903453273 39.0 36.0 40.0 32.0 41.0 18-19 37.31773980612526 39.0 36.0 40.0 32.0 41.0 20-21 37.15180831584174 39.0 36.0 40.0 31.0 41.0 22-23 37.06391797923688 39.0 36.0 40.0 31.0 41.0 24-25 37.0538027621449 39.0 36.0 40.0 31.0 41.0 26-27 36.737723956220876 38.5 35.5 40.0 30.5 41.0 28-29 36.65083576471116 38.5 35.5 40.0 30.0 41.0 30-31 36.6107798663848 38.0 35.0 40.0 30.0 41.0 32-33 36.400144979274884 38.0 35.0 40.0 30.0 41.0 34-35 36.14787294158401 38.0 35.0 40.0 30.0 41.0 36-37 35.85994298021815 38.0 35.0 40.0 29.5 41.0 38-39 35.630748316103414 38.0 34.0 40.0 29.0 41.0 40-41 35.331675759801676 38.0 34.0 40.0 27.0 41.0 42-43 34.99284604845809 38.0 33.5 40.0 26.5 41.0 44-45 34.530082109233504 38.0 33.0 40.0 25.5 41.0 46-47 34.05782037647261 37.0 33.0 40.0 24.0 41.0 48-49 33.562835774679655 37.0 32.5 40.0 22.5 41.0 50-51 33.16712521735988 37.0 32.0 39.0 21.5 40.0 52-53 32.73312879231081 36.0 31.0 39.0 19.5 40.0 54-55 32.35460080233911 36.0 31.0 39.0 18.0 40.0 56-57 31.89562021784896 35.5 30.0 39.0 15.0 40.0 58-59 31.50299304012256 35.0 30.0 38.5 13.0 40.0 60-61 30.966327987105757 35.0 29.0 38.0 8.5 40.0 62-63 30.323188210761362 34.0 28.0 38.0 7.5 40.0 64-65 29.75832291314265 34.0 28.0 37.5 6.5 39.5 66-67 29.1536518640771 33.5 26.5 37.0 2.0 39.0 68-69 28.477517932384956 33.0 26.0 36.0 2.0 39.0 70-71 27.907300799909287 32.0 25.5 36.0 2.0 38.5 72-73 27.26690360529252 32.0 24.5 35.0 2.0 38.0 74-75 26.62454697870543 31.0 23.5 35.0 2.0 37.0 76-77 25.806703126431024 30.5 23.0 34.0 2.0 36.0 78-79 26.34777456501545 31.0 24.0 34.0 2.0 36.0 80-81 26.074215744362974 31.0 23.0 34.0 2.0 36.0 82-83 25.470344043856386 31.0 20.5 34.0 2.0 35.5 84-85 24.812459308298706 31.0 19.0 34.0 2.0 35.0 86-87 24.152481189141568 30.0 17.0 34.0 2.0 35.0 88-89 23.40836431354151 30.0 8.5 34.0 2.0 35.0 90-91 22.505780791585593 29.0 3.5 34.0 2.0 35.0 92-93 21.59846130075131 29.0 2.0 33.5 2.0 35.0 94-95 20.628599794275218 27.5 2.0 33.0 2.0 34.5 96-97 19.592427247132033 26.5 2.0 33.0 2.0 34.0 98-99 18.22606175357012 24.5 2.0 32.0 2.0 34.0 100-101 15.903866342441422 11.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18128649646962813 1101 2 0.18097565059655452 1101 3 0.2390178986241196 1101 4 0.12262144885881554 1101 5 0.12526690750595293 1101 6 0.0868940728021883 1101 7 0.06857687802408208 1101 8 0.1651739944922923 1101 9 0.21766146404126374 1101 10-11 0.17301211238073222 1101 12-13 0.1619080413990659 1101 14-15 0.2561464586985309 1101 16-17 0.2804676599283482 1101 18-19 0.3081027736358948 1101 20-21 0.3307805869236162 1101 22-23 0.2449592742133646 1101 24-25 0.09606235969399535 1101 26-27 0.05439948134982586 1101 28-29 0.22247424885795652 1101 30-31 0.48371738070905934 1101 32-33 0.378953324353013 1101 34-35 0.3015825459962187 1101 36-37 0.22351891685829628 1101 38-39 0.34106321282961716 1101 40-41 0.23401770326841387 1101 42-43 0.24963269211057337 1101 44-45 0.47822579115905484 1101 46-47 0.30637968190968934 1101 48-49 0.21081637994637958 1101 50-51 0.2716954475563256 1101 52-53 0.2521439877367513 1101 54-55 0.2938501435668144 1101 56-57 0.4768386080020868 1101 58-59 0.4292340353933355 1101 60-61 0.325122034090672 1101 62-63 0.4056071294584207 1101 64-65 0.5713853961170194 1101 66-67 0.46889377021789613 1101 68-69 0.5057238179852384 1101 70-71 0.4223989903550667 1101 72-73 0.22490202165446505 1101 74-75 0.34123400754269895 1101 76-77 0.163323228663387 1101 78-79 0.2871878692792542 1101 80-81 0.5253999972659464 1101 82-83 0.4733643736715969 1101 84-85 0.6476713105735463 1101 86-87 0.6759015170667304 1101 88-89 0.909427806496744 1101 90-91 0.8680202887712234 1101 92-93 1.0613063288119555 1101 94-95 1.0545499149099804 1101 96-97 1.0634427412738496 1101 98-99 1.004707449490656 1101 100-101 1.189492939174892 1102 1 0.14756790780693052 1102 2 0.09385583432798583 1102 3 0.20632963532536763 1102 4 0.1547536044647444 1102 5 0.04149639312495168 1102 6 0.03458347139442708 1102 7 0.08250897630699683 1102 8 0.11953854653526008 1102 9 0.16237229920781715 1102 10-11 0.2806513347233093 1102 12-13 0.13504122807481167 1102 14-15 0.27904439767557676 1102 16-17 0.3665660842898859 1102 18-19 0.3654256027128113 1102 20-21 0.21239087975488502 1102 22-23 0.14036301063172374 1102 24-25 0.15998403561051333 1102 26-27 0.11636100792872384 1102 28-29 0.1413813520728766 1102 30-31 -0.00382026078387554 1102 32-33 0.07726620379228422 1102 34-35 0.0638509740428006 1102 36-37 0.3175960349250957 1102 38-39 0.46916600714050105 1102 40-41 0.4778270037415169 1102 42-43 0.42411650580333315 1102 44-45 0.4249032896422378 1102 46-47 0.328329557771859 1102 48-49 0.4439488966128593 1102 50-51 0.4871413945585914 1102 52-53 0.513156892859719 1102 54-55 0.5342792472805229 1102 56-57 0.48962517654961957 1102 58-59 0.48709212743027663 1102 60-61 0.4149416154837695 1102 62-63 0.6164006065459624 1102 64-65 0.8447253550975624 1102 66-67 0.6207856158328688 1102 68-69 0.6608964756514482 1102 70-71 0.46636239295991544 1102 72-73 0.4712852744005751 1102 74-75 0.7182134196467871 1102 76-77 0.5769984900600598 1102 78-79 0.4770813378055685 1102 80-81 0.867272970953195 1102 82-83 1.0196032021976151 1102 84-85 0.9921885875069876 1102 86-87 0.7199116342066851 1102 88-89 0.7186207058874068 1102 90-91 0.9122405282623163 1102 92-93 1.299568696996939 1102 94-95 1.3940864173871539 1102 96-97 1.3884437648991224 1102 98-99 1.5131138324456224 1102 100-101 1.3445289893958101 1103 1 0.1084343996911521 1103 2 0.23580527904581672 1103 3 0.1669572394468979 1103 4 0.0880929916309725 1103 5 0.16338604807908297 1103 6 0.19050069113273338 1103 7 0.15251175705098063 1103 8 0.21666789936043074 1103 9 0.12653725083115575 1103 10-11 0.0469623952267213 1103 12-13 0.34715622879448915 1103 14-15 0.2878265373111262 1103 16-17 0.16041348097846253 1103 18-19 0.26649121545992216 1103 20-21 0.3886378625723026 1103 22-23 0.3801516891603782 1103 24-25 0.2904890985992665 1103 26-27 0.21551280565333286 1103 28-29 0.35742763902606356 1103 30-31 0.3969062368426961 1103 32-33 0.44211827800875625 1103 34-35 0.2643066317264342 1103 36-37 0.2609023922785809 1103 38-39 0.4554617999275905 1103 40-41 0.5238179847864828 1103 42-43 0.603604487186665 1103 44-45 0.5987009193028925 1103 46-47 0.5532697039530845 1103 48-49 0.6384715468653539 1103 50-51 0.6505417546234966 1103 52-53 0.6024055870781169 1103 54-55 0.5615246980511799 1103 56-57 0.503493854595984 1103 58-59 0.5777281792862503 1103 60-61 0.7872777720132795 1103 62-63 0.36626232293570027 1103 64-65 0.7904343430800758 1103 66-67 0.8638211332434196 1103 68-69 0.858865665912365 1103 70-71 0.8990847790040455 1103 72-73 0.9567587130203599 1103 74-75 0.9171018002816567 1103 76-77 0.9283910851422839 1103 78-79 1.0607039237769023 1103 80-81 1.3150170264535284 1103 82-83 1.4152069891338428 1103 84-85 1.5009326708901227 1103 86-87 1.5023615600911882 1103 88-89 1.711982715800115 1103 90-91 1.6190069299419143 1103 92-93 1.7867239582173156 1103 94-95 1.7386720300136886 1103 96-97 1.7445704070640922 1103 98-99 1.6829641621031968 1103 100-101 1.675547171241396 1104 1 0.27082406170812945 1104 2 0.2729973360761839 1104 3 0.14522819922694552 1104 4 0.17712239962144594 1104 5 0.22182797883338168 1104 6 0.13193999261446265 1104 7 0.14199193648586572 1104 8 0.1434318736847544 1104 9 0.1786640128942807 1104 10-11 0.2418473270103405 1104 12-13 0.26528223729662415 1104 14-15 0.39534822266664804 1104 16-17 0.2850657098535052 1104 18-19 0.41584440894762764 1104 20-21 0.3154571253384688 1104 22-23 0.3919977497565199 1104 24-25 0.3996489600095714 1104 26-27 0.23585758710699878 1104 28-29 0.39544629239073714 1104 30-31 0.4045087388835853 1104 32-33 0.4215414216140019 1104 34-35 0.5654818865310673 1104 36-37 0.4436771075772299 1104 38-39 0.5421110746251401 1104 40-41 0.47260450587391034 1104 42-43 0.6416968584738143 1104 44-45 0.5777827762300589 1104 46-47 0.5120013188397436 1104 48-49 0.49043162451489053 1104 50-51 0.6111197304385527 1104 52-53 0.7264066409713692 1104 54-55 0.7776062628369473 1104 56-57 0.6970150048139949 1104 58-59 0.513710306785498 1104 60-61 0.3482923217469427 1104 62-63 0.5542983136636224 1104 64-65 0.9118757468877448 1104 66-67 0.9665593445189842 1104 68-69 1.0254531540697123 1104 70-71 0.9186910160863455 1104 72-73 0.9568638293172071 1104 74-75 1.399386291395917 1104 76-77 1.0993449950589849 1104 78-79 1.0219979190425725 1104 80-81 1.2982810103338736 1104 82-83 1.1050470212420933 1104 84-85 1.0892358208296464 1104 86-87 1.2494258120863506 1104 88-89 1.3595694094420843 1104 90-91 1.2098943026078537 1104 92-93 1.4785434530584247 1104 94-95 1.5306291316434013 1104 96-97 1.9339367332631703 1104 98-99 1.9332829238605242 1104 100-101 1.7895799238413144 1105 1 0.099879924899124 1105 2 0.08320402331673193 1105 3 0.07062672695425931 1105 4 0.03329492838475545 1105 5 0.15473962058522517 1105 6 0.1093481214857448 1105 7 0.03865707105509131 1105 8 0.04207414352359251 1105 9 0.12285626923606685 1105 10-11 0.15057225715399625 1105 12-13 0.1156444689059768 1105 14-15 0.08516852952387666 1105 16-17 0.15422750663901752 1105 18-19 0.29698574858444715 1105 20-21 0.3905882655837516 1105 22-23 0.31237464572709683 1105 24-25 0.267203513622043 1105 26-27 0.049893058810617674 1105 28-29 0.022250701913094417 1105 30-31 0.12404808825287716 1105 32-33 0.09781288009781264 1105 34-35 0.12229789621976295 1105 36-37 0.10194131413314977 1105 38-39 0.0028828139934802266 1105 40-41 0.10352660966256622 1105 42-43 0.20303912174652083 1105 44-45 0.24281666476632324 1105 46-47 0.19005365820661524 1105 48-49 0.1616041167996869 1105 50-51 -0.028634550314656337 1105 52-53 0.333809589894166 1105 54-55 0.35559877744658763 1105 56-57 0.21971704104445777 1105 58-59 0.39493554699648925 1105 60-61 0.4357174496041196 1105 62-63 0.5058271280390763 1105 64-65 0.4997510195646093 1105 66-67 0.5392223487431984 1105 68-69 0.5520051322993389 1105 70-71 0.37455639579115996 1105 72-73 0.4956776940694816 1105 74-75 0.6696599531817569 1105 76-77 0.6311360169679787 1105 78-79 0.8083928937629672 1105 80-81 0.9871273409605053 1105 82-83 0.8316923844586235 1105 84-85 1.0351491332972742 1105 86-87 1.326082483641141 1105 88-89 1.3722799816440023 1105 90-91 1.4381359654442747 1105 92-93 1.4159404924625143 1105 94-95 1.4783984707257503 1105 96-97 1.6462686585259974 1105 98-99 1.631937596392934 1105 100-101 1.3693985526870645 1106 1 -0.2527337156591649 1106 2 -0.1764932086236186 1106 3 -0.29069453721791305 1106 4 -0.13334097750641405 1106 5 -0.021493920690950574 1106 6 -0.20069889465067092 1106 7 -0.19351514553904536 1106 8 -0.045151931770583076 1106 9 -0.32613047081897406 1106 10-11 -0.4165308023810965 1106 12-13 -0.6608317407102504 1106 14-15 -0.42528165784342065 1106 16-17 -0.25529438888098355 1106 18-19 -0.29986721901010327 1106 20-21 -0.19081705069854848 1106 22-23 -0.25293649609921687 1106 24-25 -0.3544099642459244 1106 26-27 -0.33011011296712667 1106 28-29 -0.3072294683238752 1106 30-31 -0.3866126395334035 1106 32-33 -0.42559743720300247 1106 34-35 -0.6207652754759465 1106 36-37 -0.5286175339646704 1106 38-39 -0.5132259889201833 1106 40-41 -0.5078079841789886 1106 42-43 -0.7864174515577744 1106 44-45 -0.5497478405798972 1106 46-47 -0.25772862659441387 1106 48-49 -0.4325885222150916 1106 50-51 -0.44528433273343637 1106 52-53 -0.35436733514934815 1106 54-55 -0.3673123950037862 1106 56-57 -0.6217899088400891 1106 58-59 -0.5401369322263179 1106 60-61 -0.6020688135905452 1106 62-63 -0.5853247057816482 1106 64-65 -0.9302885866427815 1106 66-67 -1.005944176423906 1106 68-69 -0.8803009278468643 1106 70-71 -0.7110492018496899 1106 72-73 -0.6219020207712944 1106 74-75 -0.7983020839198396 1106 76-77 -0.9867631997943214 1106 78-79 -0.8319287409056564 1106 80-81 -0.9678987646843034 1106 82-83 -0.8444353397498325 1106 84-85 -0.2517839626995908 1106 86-87 0.08308493493315794 1106 88-89 -0.3166398503064656 1106 90-91 -0.5363943858711195 1106 92-93 -0.5399548443406381 1106 94-95 -0.21058643735421612 1106 96-97 -0.11856876735762256 1106 98-99 -0.25920412055051045 1106 100-101 0.08180649252171968 1107 1 0.03625576508207473 1107 2 0.09308128609049504 1107 3 -0.018847973634557036 1107 4 0.04408344513340268 1107 5 0.07893747481970337 1107 6 0.07700551058978533 1107 7 0.08274252247458236 1107 8 0.01777107257662891 1107 9 0.15001411888267313 1107 10-11 0.05194861815382268 1107 12-13 0.004130436694921968 1107 14-15 -7.728089403755689E-4 1107 16-17 0.025849189942562134 1107 18-19 0.19567879082211448 1107 20-21 0.09727883130791781 1107 22-23 -0.09793518764661258 1107 24-25 0.08862289760686082 1107 26-27 0.0684126046582989 1107 28-29 0.10624242970259701 1107 30-31 0.2113029385794718 1107 32-33 0.18543417483686397 1107 34-35 0.21671134523295876 1107 36-37 0.09703767695876309 1107 38-39 0.21862962442901335 1107 40-41 0.37797591896575966 1107 42-43 0.4622935694641157 1107 44-45 0.4300372231054226 1107 46-47 0.16903828767174645 1107 48-49 0.34232043311460103 1107 50-51 0.3184743308419584 1107 52-53 0.42037707898334276 1107 54-55 0.5415834197858445 1107 56-57 0.5829842364865492 1107 58-59 0.6300686830045983 1107 60-61 0.5988793112962263 1107 62-63 0.48183654721707114 1107 64-65 0.6550085196202033 1107 66-67 0.7794936650756625 1107 68-69 0.6888864574447275 1107 70-71 0.694294536088691 1107 72-73 0.8098588938677089 1107 74-75 0.9558510496709829 1107 76-77 0.6571552087772261 1107 78-79 0.8536111511448148 1107 80-81 1.096123077284382 1107 82-83 1.0778303225153572 1107 84-85 1.3335695951974706 1107 86-87 1.3941028060326381 1107 88-89 1.6018488037903005 1107 90-91 1.4905343564127271 1107 92-93 1.3404709672719477 1107 94-95 1.4451224857674063 1107 96-97 1.5406327177141996 1107 98-99 1.4241866709189033 1107 100-101 1.431663087866779 1108 1 0.00867864455143419 1108 2 0.01736914216246177 1108 3 0.0072071806065068245 1108 4 0.06682207581806665 1108 5 -0.10537945762316525 1108 6 -0.05629147727991324 1108 7 -0.11940640306264783 1108 8 -0.02348102864549162 1108 9 0.004450537037229196 1108 10-11 -0.03708640978675248 1108 12-13 0.14103925501303394 1108 14-15 -0.17211895404964395 1108 16-17 -0.1213385798089206 1108 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0.03661884165704521 2316 48-49 0.2139655531775233 2316 50-51 0.14064726077042877 2316 52-53 -0.16000383711598687 2316 54-55 0.11849885551060879 2316 56-57 0.21632125650112854 2316 58-59 0.584377389307182 2316 60-61 0.7477393075276169 2316 62-63 0.6750503911147945 2316 64-65 0.4850945012195673 2316 66-67 0.7059085314129838 2316 68-69 0.5687303469753751 2316 70-71 0.7865977697177797 2316 72-73 1.1842608322427814 2316 74-75 1.192259484797681 2316 76-77 1.0305310208711767 2316 78-79 1.0621789983687435 2316 80-81 1.2518228913117895 2316 82-83 0.8223832509691604 2316 84-85 0.9818704447726816 2316 86-87 1.3988381432820454 2316 88-89 1.7825258832833448 2316 90-91 1.689638697684714 2316 92-93 1.916935808621762 2316 94-95 1.543425793130119 2316 96-97 2.128431031671976 2316 98-99 1.7459110546286887 2316 100-101 1.9481456252865978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 43.0 5 99.0 6 219.0 7 533.0 8 1163.0 9 2075.0 10 3126.0 11 4888.0 12 7201.0 13 9540.0 14 12306.0 15 16130.0 16 20954.0 17 26231.0 18 24518.0 19 20543.0 20 20174.0 21 21201.0 22 23117.0 23 25563.0 24 29212.0 25 34420.0 26 40426.0 27 48035.0 28 56961.0 29 67549.0 30 79227.0 31 92754.0 32 109504.0 33 129074.0 34 153100.0 35 177132.0 36 179373.0 37 131751.0 38 36099.0 39 800.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.06846603621425 21.70174789132486 16.453399743979976 20.77638632848092 2 17.893383225642452 22.398020755649174 36.811652171854334 22.896943846854036 3 21.144233506972025 24.83880634768734 30.496237828313888 23.520722317026745 4 15.619819109227898 18.447008423999893 34.69141594348363 31.241756523288583 5 14.107950520237841 37.7985082299078 33.49529172136761 14.598249528486754 6 32.762138665480414 36.9300903332405 15.613526497812849 14.694244503466233 7 28.544157621816545 33.74920641447625 19.569086954544293 18.137549009162914 8 27.302830989802857 35.56521668700862 18.39816280667914 18.733789516509383 9 26.553947928329023 16.625577783218915 19.59338515703804 37.227089131414026 10-11 25.720955704376852 27.103928396312405 25.877492201211545 21.297623698099194 12-13 26.45483690181835 24.22863683135104 27.21570941299359 22.100816853837028 14-15 23.161108920119347 25.503455640516194 25.680707912553885 25.65472752681057 16-17 22.18734287941176 28.929315282884033 26.511145710090045 22.37219612761416 18-19 22.318241657461385 26.656966076594163 29.67788682894128 21.346905437003173 20-21 22.68212121674676 25.7194711319052 30.22696170618931 21.371445945158733 22-23 22.635367196614396 25.58107078828622 30.259948007283345 21.523614007816043 24-25 22.6209723393001 25.952536265067224 29.999682254275083 21.426809141357598 26-27 22.190177220489655 26.211991081811636 29.98405953267671 21.613772165021995 28-29 22.21021830412674 26.238893130757866 30.079596568665472 21.471291996449914 30-31 22.13851105506599 26.182887076837197 30.00322107141433 21.675380796682482 32-33 22.10986899530671 26.135271208436833 29.87062141718331 21.884238379073143 34-35 22.596393772931428 26.152342253265772 29.75965962579522 21.491604348007577 36-37 22.623884654168418 25.938918470840346 29.69029342672182 21.746903448269418 38-39 22.3555923559101 26.23744826507719 29.68866526235517 21.71829411665754 40-41 22.570704786256414 26.117625949033492 29.672989195379095 21.638680069331002 42-43 22.463694738249103 26.208646087687228 29.377499009976876 21.95016016408679 44-45 22.52577322799128 26.375823413124895 29.33154398878046 21.76685937010337 46-47 22.79962643071243 26.75549840286046 28.60253561088485 21.842339555542264 48-49 22.90127391114459 26.879948811786747 28.23497857085449 21.983798706214174 50-51 22.81542026233336 27.188629438082263 28.1373496392963 21.858600660288076 52-53 23.272794446799846 27.06104115337996 27.843100850343898 21.823063549476295 54-55 23.077155515349297 27.22523249953117 27.83651920149876 21.86109278362077 56-57 23.00170648145206 27.41513229748227 27.763406533225922 21.819754687839747 58-59 22.998186357244634 26.9444636545618 27.86648698457438 22.190863003619185 60-61 22.777446533051474 27.161652203005875 28.045701803736566 22.015199460206087 62-63 22.783240719799984 27.2102174564517 28.24999361393396 21.756548209814355 64-65 22.641283144461536 27.1035545778125 28.131119330964573 22.124042946761392 66-67 22.611876089135773 27.331988832795346 27.979972050836828 22.076163027232056 68-69 22.810342561043004 27.048852470659924 28.019441054118328 22.121363914178747 70-71 22.80445491966952 27.191775743789787 27.795772985009254 22.207996351531442 72-73 22.864639698154022 27.280526485975265 27.555688053446076 22.299145762424637 74-75 22.818068143374347 27.633566907592687 27.264483442021064 22.283881507011902 76-77 22.909155251184227 27.36821807574435 27.624844475928267 22.097782197143154 78-79 22.869623944819406 27.467186523593867 27.47646968300814 22.186719848578587 80-81 22.92747235767951 27.61792883368005 27.338624111168635 22.115974697471803 82-83 22.948303033758023 27.47612154221458 27.314164525774963 22.26141089825243 84-85 22.903904910482876 27.533556461581405 27.251198712069858 22.31133991586586 86-87 23.14008162949976 27.506375163000442 27.172804454919667 22.180738752580126 88-89 22.92476217355521 27.473634892717207 27.126326354764974 22.475276578962617 90-91 22.98867584529263 27.422412031244548 27.164761653149625 22.424150470313194 92-93 23.014891719384096 27.29994869221215 27.214373407924324 22.470786180479426 94-95 22.918064592098595 27.185701193166352 27.17261754566972 22.723616669065336 96-97 23.14104732729118 26.99334042342422 27.14364661192718 22.721965637357428 98-99 22.88892211744842 27.119177343937395 26.943263272992347 23.048637265621835 100-101 23.243785012996536 26.735543279576508 27.291825624879078 22.728846082547875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1928.0 1 1230.0 2 706.0 3 946.0 4 826.5 5 802.5 6 1452.5 7 2905.0 8 4244.0 9 4702.0 10 3976.5 11 3014.0 12 2870.0 13 2928.5 14 3011.5 15 3141.0 16 3195.0 17 3210.0 18 3323.0 19 3520.0 20 3789.0 21 4202.5 22 4804.0 23 5554.0 24 6489.0 25 7701.0 26 9174.0 27 10900.0 28 12508.0 29 14394.0 30 16924.5 31 19588.5 32 22887.0 33 24833.0 34 25915.5 35 28096.5 36 31408.5 37 35206.5 38 38694.0 39 40877.5 40 42581.0 41 45987.0 42 48763.5 43 51360.0 44 54690.5 45 57080.0 46 57701.0 47 58830.0 48 60655.5 49 60346.5 50 59186.0 51 57782.5 52 56622.0 53 56854.5 54 56717.0 55 53342.5 56 48651.5 57 44347.5 58 40477.5 59 36497.5 60 30673.5 61 25227.0 62 20733.0 63 15929.0 64 11792.5 65 8944.5 66 6945.5 67 5389.0 68 4023.5 69 2978.5 70 2228.0 71 1604.5 72 1216.0 73 1018.5 74 816.0 75 656.0 76 495.5 77 328.0 78 226.0 79 171.0 80 106.5 81 65.0 82 47.5 83 29.5 84 12.0 85 5.0 86 2.5 87 0.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030840026242058696 2 0.0 3 0.0 4 0.0 5 0.007351763831440253 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035824272907441918 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.049700415623868E-4 22-23 0.0015264255412736122 24-25 0.0 26-27 0.0014018193746390315 28-29 0.0015887286245909024 30-31 6.230308331729029E-5 32-33 0.0 34-35 0.0 36-37 3.426669582450966E-4 38-39 0.0 40-41 1.8690924995187086E-4 42-43 0.01847286420357657 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.115154165864514E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.345462497593543E-5 84-85 6.230308331729029E-5 86-87 0.0 88-89 0.0 90-91 0.01052922108062206 92-93 0.002336365624398386 94-95 0.0 96-97 0.0 98-99 7.476369998074834E-4 100-101 0.012865586705020445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1605057.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.222959389021035 #Duplication Level Percentage of deduplicated Percentage of total 1 72.51319541800186 36.41827268646453 2 13.97306548406973 14.035374006931312 3 5.358651513965774 8.073820120974574 4 2.6473450806947403 5.318300179058263 5 1.4093212338351688 3.539014154649435 6 0.9148471088731405 2.7567797517659423 7 0.6120260268490726 2.151643080402541 8 0.4252414761239144 1.708550830871897 9 0.3352017918736541 1.5151343382357854 >10 1.681569148957756 14.932973311170247 >50 0.08663118805020624 2.9137071026910504 >100 0.039060666316017484 3.4634956236482264 >500 0.002851897901563724 1.0145096473239312 >1k 6.19977804687766E-4 0.5393571944922084 >5k 2.479911218751064E-4 0.986423964987482 >10k+ 1.239955609375532E-4 0.632644006332565 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10159 0.6329370234203521 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9327 0.5811008581003666 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6513 0.4057799816455116 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2267 0.1412410898802971 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2174 0.1354469031317891 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1841 0.11469997638713142 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.230308331729029E-5 0.0 18-19 0.0 0.0 0.0 6.230308331729029E-5 0.0 20-21 0.0 0.0 0.0 6.230308331729029E-5 0.0 22-23 0.0 0.0 0.0 1.557577082932257E-4 0.0 24-25 0.0 0.0 0.0 1.8690924995187086E-4 0.0 26-27 0.0 0.0 0.0 3.115154165864514E-4 6.230308331729029E-5 28-29 0.0 0.0 0.0 7.787885414661285E-4 6.230308331729029E-5 30-31 0.0 0.0 0.0 0.0019002440411773538 1.2460616663458057E-4 32-33 0.0 0.0 0.0 0.003707033457378772 1.2460616663458057E-4 34-35 0.0 0.0 0.0 0.0063237629567049645 1.2460616663458057E-4 36-37 0.0 0.0 0.0 0.011775282746967864 1.2460616663458057E-4 38-39 0.0 0.0 0.0 0.020061592828167475 1.2460616663458057E-4 40-41 0.0 0.0 0.0 0.02996778307561663 1.2460616663458057E-4 42-43 0.0 0.0 0.0 0.03968706407311391 1.2460616663458057E-4 44-45 0.0 0.0 0.0 0.05295762081969675 1.2460616663458057E-4 46-47 0.0 0.0 0.0 0.07083860573175907 1.2460616663458057E-4 48-49 0.0 0.0 0.0 0.09049522851836414 1.2460616663458057E-4 50-51 0.0 0.0 0.0 0.11174057992956013 1.2460616663458057E-4 52-53 0.0 0.0 0.0 0.13491732692359212 2.4921233326916114E-4 54-55 0.0 0.0 0.0 0.16173880429168558 2.4921233326916114E-4 56-57 0.0 0.0 0.0 0.1965039247827336 2.8036387492780626E-4 58-59 0.0 0.0 0.0 0.232546258481786 3.115154165864514E-4 60-61 0.0 0.0 0.0 0.27139223093011655 3.115154165864514E-4 62-63 0.0 0.0 0.0 0.3149420861689024 3.115154165864514E-4 64-65 0.0 0.0 0.0 0.36238588411501893 3.115154165864514E-4 66-67 0.0 0.0 0.0 0.41730605205921034 3.115154165864514E-4 68-69 0.0 0.0 0.0 0.482475077209096 3.115154165864514E-4 70-71 0.0 0.0 0.0 0.5541547745656386 3.738184999037417E-4 72-73 0.0 0.0 0.0 0.6316909617540063 3.738184999037417E-4 74-75 0.0 0.0 0.0 0.7136818193995602 3.738184999037417E-4 76-77 0.0 0.0 0.0 0.8089743853333557 3.738184999037417E-4 78-79 0.0 0.0 0.0 0.921057632221161 3.738184999037417E-4 80-81 0.0 0.0 0.0 1.0427044023981704 3.738184999037417E-4 82-83 0.0 0.0 0.0 1.1712668148233987 3.738184999037417E-4 84-85 0.0 0.0 0.0 1.3062464448303082 4.3612158322103205E-4 86-87 0.0 0.0 0.0 1.4558984509584394 4.3612158322103205E-4 88-89 0.0 0.0 0.0 1.6182914376249566 4.3612158322103205E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4360 0.0 53.07245 1 GTATCAA 7670 0.0 40.328518 1 ACCGTAT 110 7.014023E-8 34.546352 8 GTAAGGT 690 0.0 30.976168 4 ATCAACG 10260 0.0 29.859093 3 TAAGGTG 830 0.0 29.757027 5 TATCAAC 10320 0.0 29.685495 2 TCAACGC 10460 0.0 29.37899 4 TACCGTA 130 3.0374576E-7 29.231527 7 CAACGCA 10705 0.0 28.61787 5 AACGCAG 10990 0.0 27.875732 6 GGTAAGG 775 0.0 26.96592 3 AGGTAAG 755 0.0 25.792961 2 ACGCAGA 12630 0.0 24.14552 7 CGCAGAG 12790 0.0 23.843464 8 TGGTATC 2035 0.0 23.106546 2 GAGTACT 8845 0.0 22.475143 12-13 GCAGAGT 13660 0.0 22.22056 9 AGTACTT 9150 0.0 21.725973 12-13 GTGGTAT 2245 0.0 21.376257 1 >>END_MODULE