FastQCFastQC Report
Thu 26 May 2016
SRR937536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357048
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90690.6682888151340262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68140.5021193060230736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.3705101072327582No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18920.1394202710589456No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.11922938613814693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.1121552074797649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30500.052.7037351
GTATCAA55550.042.121671
ATCAACG73150.031.7141723
TATCAAC74350.031.2662492
TCAACGC74550.031.1846754
CAACGCA76200.030.5060355
AACGCAG78150.029.7891526
TGGTATC15750.029.2778432
GTGGTAT16250.029.2665251
TACGCGC350.00841008127.13190758-59
ACGCAGA89700.025.7415647
GTACATG52000.025.6996691
CGCAGAG90050.025.535998
TACATGG53750.024.2337132
GGACCGT1401.7766954E-523.7553316
ATACCGT2002.0498192E-823.7553316
GCAGAGT95850.023.5942349
ACATGGG53450.023.4803263
GAGTACT61700.021.7853212-13
AGAGTAC89050.021.57642210-11
Produced by FastQC (version 0.11.5)