##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1357048 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24063850357541 33.0 31.0 34.0 31.0 34.0 2 32.38157456479063 34.0 31.0 34.0 31.0 34.0 3 32.43023459744976 34.0 31.0 34.0 31.0 34.0 4 36.005559862289324 37.0 35.0 37.0 35.0 37.0 5 35.99892708290348 37.0 37.0 37.0 35.0 37.0 6 36.07108591589981 37.0 37.0 37.0 35.0 37.0 7 36.005930519775276 37.0 37.0 37.0 35.0 37.0 8 36.01628903325454 37.0 37.0 37.0 35.0 37.0 9 37.67457304384222 39.0 38.0 39.0 35.0 39.0 10-11 37.618353219635566 39.0 38.0 39.0 35.0 39.0 12-13 37.53332527662987 39.0 38.0 39.0 35.0 39.0 14-15 38.78346197039456 40.0 38.0 41.0 36.0 41.0 16-17 38.69849629489893 40.0 38.0 41.0 36.0 41.0 18-19 38.62385634111689 40.0 38.0 41.0 35.0 41.0 20-21 38.624246157836716 40.0 38.0 41.0 35.0 41.0 22-23 38.62174477247673 40.0 38.0 41.0 35.0 41.0 24-25 38.55181762177904 40.0 38.0 41.0 35.0 41.0 26-27 38.445153377036036 40.0 38.0 41.0 34.5 41.0 28-29 38.291253146535716 40.0 38.0 41.0 34.0 41.0 30-31 38.03474011236153 40.0 38.0 41.0 34.0 41.0 32-33 37.89018221905194 40.0 37.5 41.0 34.0 41.0 34-35 37.893496029617225 40.0 38.0 41.0 34.0 41.0 36-37 37.748443312248355 40.0 37.5 41.0 33.5 41.0 38-39 37.58534849172616 40.0 37.0 41.0 33.0 41.0 40-41 37.356947211889334 40.0 37.0 41.0 33.0 41.0 42-43 37.09358290937388 40.0 37.0 41.0 32.0 41.0 44-45 36.83640556561006 40.0 36.0 41.0 31.5 41.0 46-47 36.44975822520648 39.0 36.0 41.0 30.5 41.0 48-49 36.07494392239626 39.0 35.5 41.0 30.0 41.0 50-51 35.1801583289611 38.0 34.5 39.5 28.0 40.5 52-53 35.275197340108825 38.5 35.0 40.0 28.5 40.5 54-55 35.518458448043106 39.0 35.0 40.0 28.5 41.0 56-57 35.263613372555724 38.5 35.0 40.0 28.0 41.0 58-59 34.96738324657639 38.0 35.0 40.0 27.0 41.0 60-61 34.60503607831116 38.0 34.0 40.0 26.0 41.0 62-63 34.22717140440132 37.0 34.0 40.0 26.0 41.0 64-65 33.7741384239909 36.5 33.5 39.5 25.0 41.0 66-67 33.245985035164566 36.0 33.0 39.0 22.5 40.5 68-69 32.785328890356126 35.5 32.5 39.0 22.0 40.0 70-71 32.32268423814043 35.0 32.0 38.0 20.5 40.0 72-73 31.854788850504917 35.0 31.5 37.0 19.0 39.5 74-75 31.316930572831616 35.0 31.0 37.0 17.0 39.0 76-77 30.790550518478344 34.5 31.0 36.0 12.0 39.0 78-79 30.28653002694083 34.0 30.0 36.0 7.5 37.5 80-81 29.777482447194203 34.0 29.5 35.0 4.0 37.0 82-83 29.266459992572113 34.0 29.0 35.0 2.0 36.5 84-85 28.757377410379 34.0 29.0 35.0 2.0 36.0 86-87 28.19996492386415 33.5 27.5 35.0 2.0 36.0 88-89 27.675496371535864 33.0 26.5 35.0 2.0 35.0 90-91 27.12227644121652 33.0 25.5 35.0 2.0 35.0 92-93 26.468172459632967 32.0 25.0 34.5 2.0 35.0 94-95 25.710447603916737 32.0 23.5 34.0 2.0 35.0 96-97 24.841262431395204 31.5 19.0 34.0 2.0 35.0 98-99 23.649620352412 31.0 2.0 34.0 2.0 35.0 100-101 20.396988905329806 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07810451342722757 1101 2 0.11448407106330194 1101 3 0.1378618463603658 1101 4 0.08780682842667886 1101 5 0.030743535137943923 1101 6 0.08352614544607206 1101 7 0.054662007579096894 1101 8 0.026936478401900388 1101 9 0.2057220745976167 1101 10-11 0.24004341711902555 1101 12-13 0.197993963191756 1101 14-15 0.34018682823778335 1101 16-17 0.3021118374059668 1101 18-19 0.36819356626518385 1101 20-21 0.3399649553332793 1101 22-23 0.23363017499065108 1101 24-25 0.18240557866725027 1101 26-27 0.28420107325151633 1101 28-29 0.2740673749039786 1101 30-31 0.26930306278470795 1101 32-33 0.30172962136914094 1101 34-35 0.35337560917280086 1101 36-37 0.3957836318164354 1101 38-39 0.2944307003698512 1101 40-41 0.31286948930085856 1101 42-43 0.35683099452169387 1101 44-45 0.5167344231817239 1101 46-47 0.4382328673012239 1101 48-49 0.39170075216563305 1101 50-51 0.29916818795867783 1101 52-53 0.43151360168031516 1101 54-55 0.5450467646898289 1101 56-57 0.7400750953702797 1101 58-59 0.6008921259763582 1101 60-61 0.5777563061409765 1101 62-63 0.584327991081544 1101 64-65 0.5861180997721931 1101 66-67 0.5165257884137517 1101 68-69 0.714223854958874 1101 70-71 0.7712249910112732 1101 72-73 0.8302440739935939 1101 74-75 0.8038803736479565 1101 76-77 0.7932294919593303 1101 78-79 0.9876501566395177 1101 80-81 1.2082371342264047 1101 82-83 1.2083821051038655 1101 84-85 1.1259789519442691 1101 86-87 1.269448861087728 1101 88-89 1.0940311859040328 1101 90-91 1.2144413662175815 1101 92-93 1.3300079866639116 1101 94-95 1.490071272842215 1101 96-97 1.5317126862999757 1101 98-99 1.8727892937610875 1101 100-101 2.446468695158213 1102 1 -0.02416990807731878 1102 2 -0.04281561143766055 1102 3 -0.0023612500854781615 1102 4 -0.07960487668210448 1102 5 -0.08405549508375998 1102 6 0.045271238103609335 1102 7 0.008092234030122825 1102 8 0.06449251511929077 1102 9 0.05345637838693307 1102 10-11 0.11357621236433602 1102 12-13 0.16443800779749296 1102 14-15 0.22267596821182423 1102 16-17 0.3093703500737419 1102 18-19 0.2092456079839451 1102 20-21 0.16805019383029673 1102 22-23 0.1489719523753621 1102 24-25 0.23906895005303141 1102 26-27 0.301573302758932 1102 28-29 0.201367040462614 1102 30-31 0.3303343755771806 1102 32-33 0.3124394960298744 1102 34-35 0.40729601924467573 1102 36-37 0.4371872689933056 1102 38-39 0.4688084513787629 1102 40-41 0.4002956631493788 1102 42-43 0.52816378739508 1102 44-45 0.6826691817028561 1102 46-47 0.5845971305306321 1102 48-49 0.5226170096753151 1102 50-51 0.4006350962228069 1102 52-53 0.416947020064093 1102 54-55 0.48294427383021343 1102 56-57 0.48818750934565713 1102 58-59 0.5084241300154133 1102 60-61 0.5861772736151138 1102 62-63 0.6035835710340436 1102 64-65 0.5837548581645677 1102 66-67 0.6151739184900933 1102 68-69 0.6387473099041685 1102 70-71 0.7606154995857821 1102 72-73 0.8027212781044746 1102 74-75 0.7045632337823449 1102 76-77 0.5604308158320741 1102 78-79 0.7757180631450318 1102 80-81 0.9742391537507267 1102 82-83 1.1287419853555605 1102 84-85 1.1440983376835838 1102 86-87 1.1178894477663235 1102 88-89 1.0816694352204088 1102 90-91 1.1195406651444593 1102 92-93 1.1635285326131743 1102 94-95 1.3485654311309077 1102 96-97 1.311145940343259 1102 98-99 1.4538673927652184 1102 100-101 2.0199557885002193 1103 1 0.05681476381644046 1103 2 -0.038160670419081555 1103 3 0.09191013509499868 1103 4 0.05910388929938648 1103 5 0.0289133542487221 1103 6 -0.019488244837695845 1103 7 0.03460915145613086 1103 8 0.05093031973891726 1103 9 0.04901334667117396 1103 10-11 0.02555615161700331 1103 12-13 0.04102202983605707 1103 14-15 0.15657792484105215 1103 16-17 0.12439118782184977 1103 18-19 0.07764993093372397 1103 20-21 0.05543805849088557 1103 22-23 4.3059474310069845E-4 1103 24-25 -0.10440071159845132 1103 26-27 -0.122337130242407 1103 28-29 -0.01810742446639324 1103 30-31 0.07555935262033842 1103 32-33 0.06196270009853322 1103 34-35 0.045958871543888336 1103 36-37 0.1175750630174619 1103 38-39 0.1563058025124704 1103 40-41 0.11839271761007097 1103 42-43 -0.020243361012070693 1103 44-45 0.2273714491323986 1103 46-47 0.07930646160360055 1103 48-49 0.08917772169057514 1103 50-51 0.011015120239200371 1103 52-53 0.03432005107348601 1103 54-55 0.2227373090777931 1103 56-57 0.14993788018693976 1103 58-59 0.11998731430586673 1103 60-61 0.31685467701979064 1103 62-63 0.3377504142917971 1103 64-65 0.24413135035924682 1103 66-67 0.4381025050550349 1103 68-69 0.5560850226964291 1103 70-71 0.5126922107534 1103 72-73 0.4257558193208446 1103 74-75 0.49545113376115424 1103 76-77 0.6732442650091599 1103 78-79 0.8111245758242696 1103 80-81 0.8541995973475345 1103 82-83 0.9905029516619841 1103 84-85 1.1807727743580188 1103 86-87 1.2672678471447227 1103 88-89 1.3927840781532375 1103 90-91 1.4507796282016017 1103 92-93 1.3848854585155514 1103 94-95 1.5342015122215322 1103 96-97 1.7025318271836944 1103 98-99 1.8538603291217939 1103 100-101 2.4007921238820096 1104 1 0.11574559135024742 1104 2 0.07708081716997839 1104 3 0.07590802230939886 1104 4 0.10475012976079512 1104 5 0.12689065299414892 1104 6 0.14219782073699605 1104 7 0.15770867954845613 1104 8 0.1737080061267804 1104 9 0.31296875436157734 1104 10-11 0.1921736915422727 1104 12-13 0.160414223496133 1104 14-15 0.277766545789639 1104 16-17 0.32821512832366295 1104 18-19 0.40844971204555947 1104 20-21 0.29851415346328736 1104 22-23 0.19127668256470542 1104 24-25 0.20148431392171773 1104 26-27 0.21381601090353541 1104 28-29 0.3059450952654288 1104 30-31 0.30236946041940627 1104 32-33 0.3480465154670327 1104 34-35 0.43319867606645346 1104 36-37 0.2977252856544794 1104 38-39 0.3436360270966574 1104 40-41 0.40383408053583025 1104 42-43 0.47750598517702514 1104 44-45 0.419902841567783 1104 46-47 0.39327770399538764 1104 48-49 0.4804416665790967 1104 50-51 0.500821153540187 1104 52-53 0.589180968680445 1104 54-55 0.5577464350134704 1104 56-57 0.6142685087518416 1104 58-59 0.6823179585376167 1104 60-61 0.7195482548453924 1104 62-63 0.6235845673366995 1104 64-65 0.6553695121014869 1104 66-67 0.7294288935009661 1104 68-69 0.6586776612948242 1104 70-71 0.7426189011217588 1104 72-73 0.7973729843609512 1104 74-75 0.7566687607493066 1104 76-77 0.7728511391719621 1104 78-79 0.9893298718884225 1104 80-81 1.0800588397214206 1104 82-83 1.1144682966066846 1104 84-85 1.2138598025482636 1104 86-87 1.5134812272995326 1104 88-89 1.5723572014613545 1104 90-91 1.530882402687478 1104 92-93 1.6258717086232615 1104 94-95 1.7482074454785455 1104 96-97 1.8475322580218538 1104 98-99 2.2310471685265867 1104 100-101 2.8846748598050738 1105 1 0.11304252728105268 1105 2 0.12117023989867448 1105 3 0.10001266852648172 1105 4 0.10612956099610926 1105 5 0.06698843706065105 1105 6 0.07605908064384437 1105 7 0.1214144514193265 1105 8 0.057628117555132974 1105 9 0.18490694868319935 1105 10-11 0.14548622154707402 1105 12-13 0.106557316917403 1105 14-15 0.1385912514267531 1105 16-17 0.16771984381498584 1105 18-19 0.23512869120853708 1105 20-21 0.14364042629852491 1105 22-23 0.1393127104344245 1105 24-25 0.08032163841995299 1105 26-27 0.09364020382703586 1105 28-29 0.2396604107685505 1105 30-31 0.1728330767032773 1105 32-33 0.2857657610726463 1105 34-35 0.41160495791366003 1105 36-37 0.3267633527978475 1105 38-39 0.29802767712909173 1105 40-41 0.42643927631451817 1105 42-43 0.4148347735726432 1105 44-45 0.4509651799629708 1105 46-47 0.5392860653332008 1105 48-49 0.5243954097043115 1105 50-51 0.33528361490242986 1105 52-53 0.48594063443749036 1105 54-55 0.6252646063419292 1105 56-57 0.6024785960500267 1105 58-59 0.5915709390144883 1105 60-61 0.6443539869204074 1105 62-63 0.6765485678925742 1105 64-65 0.6447045162284653 1105 66-67 0.8435907120235058 1105 68-69 0.8055310262542719 1105 70-71 0.6439207409950853 1105 72-73 0.6014041293441501 1105 74-75 0.8059954688120676 1105 76-77 0.8675835331971093 1105 78-79 1.1741801058205894 1105 80-81 1.2562511094319468 1105 82-83 1.255801304624807 1105 84-85 1.4600456396506232 1105 86-87 1.5625498677556884 1105 88-89 1.5358524133268325 1105 90-91 1.4124480584555563 1105 92-93 1.598743420728777 1105 94-95 1.5829827200487934 1105 96-97 1.6507183993834218 1105 98-99 1.5152067130010707 1105 100-101 2.080959818029971 1106 1 0.007630216623930153 1106 2 -0.16496560519214398 1106 3 -0.12612070524659913 1106 4 -0.05414381916745015 1106 5 -0.07208684077537697 1106 6 -0.1070153259902682 1106 7 -0.07246916199668618 1106 8 -0.0354759698546232 1106 9 0.0041771735481503924 1106 10-11 -0.3082701814125173 1106 12-13 -0.45414707728524206 1106 14-15 -0.5980154233777952 1106 16-17 -0.44956805346806306 1106 18-19 -0.1538874559821508 1106 20-21 -0.13266600212313762 1106 22-23 -0.196564176753661 1106 24-25 -0.401952429468885 1106 26-27 -0.3439087856786003 1106 28-29 -0.1892060469594412 1106 30-31 -0.05466779075421613 1106 32-33 -0.08828346807592169 1106 34-35 -0.10924740955880452 1106 36-37 -0.33513822634922263 1106 38-39 -0.520488983047251 1106 40-41 -0.1617599318652836 1106 42-43 -0.18519633632360666 1106 44-45 -0.17702349191592504 1106 46-47 -0.11316958824296819 1106 48-49 -0.017390610375770166 1106 50-51 -0.11943822455678088 1106 52-53 -0.01965930290587181 1106 54-55 -0.13821780486536994 1106 56-57 -0.4145243508091596 1106 58-59 -0.4750346623894188 1106 60-61 -0.11544781464525045 1106 62-63 -0.3541060691981599 1106 64-65 -0.06257429459341068 1106 66-67 0.04929967441953664 1106 68-69 -0.16241626353394878 1106 70-71 -0.13496902575245429 1106 72-73 -0.16165968681976395 1106 74-75 0.03168528473634069 1106 76-77 0.2564108991718079 1106 78-79 0.36680262951029263 1106 80-81 0.1631696445645936 1106 82-83 0.3079546898308507 1106 84-85 0.5665758159379521 1106 86-87 0.6024950369535844 1106 88-89 0.5285058596917516 1106 90-91 0.8236970162584321 1106 92-93 0.8264744140487323 1106 94-95 0.859512721596488 1106 96-97 0.9843072894372433 1106 98-99 0.8036823530474031 1106 100-101 1.4665563729131925 1107 1 -0.055903233718311185 1107 2 0.03739390880041782 1107 3 0.05276882265525984 1107 4 0.08720121373178813 1107 5 0.07812557309445367 1107 6 0.11100232334921145 1107 7 -0.003359744104386664 1107 8 0.08982917539169932 1107 9 0.15900736661284043 1107 10-11 0.09094869973206698 1107 12-13 0.2155103687223061 1107 14-15 0.3007136030081412 1107 16-17 0.19456330773218866 1107 18-19 0.21782738001169122 1107 20-21 0.1659816742701068 1107 22-23 0.17110186646149117 1107 24-25 0.17351334642032867 1107 26-27 0.3026021538591763 1107 28-29 0.19892231717067688 1107 30-31 0.10325515885018177 1107 32-33 0.17415998507068764 1107 34-35 0.11521649001616652 1107 36-37 0.20002867688468484 1107 38-39 0.2987739800591882 1107 40-41 0.32293471429866116 1107 42-43 0.3392854754801178 1107 44-45 0.24595193786995395 1107 46-47 0.330680044390796 1107 48-49 0.49415052666337544 1107 50-51 0.24531583988022732 1107 52-53 0.35132050262890857 1107 54-55 0.5524866754763735 1107 56-57 0.52523576438535 1107 58-59 0.37890187269687203 1107 60-61 0.4786551220663071 1107 62-63 0.4770589242809322 1107 64-65 0.47640698629979994 1107 66-67 0.5362071926885292 1107 68-69 0.641575448500987 1107 70-71 0.6443637387600063 1107 72-73 0.8343247954290867 1107 74-75 0.7379359903821339 1107 76-77 0.7514870228330714 1107 78-79 0.8695033698051553 1107 80-81 1.0627323676604128 1107 82-83 1.1966209174658111 1107 84-85 1.2795364888285405 1107 86-87 1.390815038754738 1107 88-89 1.3986168724677057 1107 90-91 1.5793036928513224 1107 92-93 1.6328829525863462 1107 94-95 1.744619017926997 1107 96-97 1.965515672890053 1107 98-99 2.20835900089795 1107 100-101 2.912137962452981 1108 1 0.015474363240372213 1108 2 0.01130112044398146 1108 3 0.12147526211235515 1108 4 0.0733081563698974 1108 5 0.10649564456986838 1108 6 0.15606592498399152 1108 7 0.17291182542759032 1108 8 0.15823363213777242 1108 9 0.20849547812782987 1108 10-11 0.22128239989102383 1108 12-13 0.1789973241807843 1108 14-15 0.25046164197318177 1108 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-0.5179712087471344 2316 52-53 -0.558863342109909 2316 54-55 -0.6427260563855697 2316 56-57 -0.5575276272785672 2316 58-59 -0.6545417602232035 2316 60-61 -0.6429386827735755 2316 62-63 -0.6903098137684296 2316 64-65 -0.6608204545925602 2316 66-67 -0.503420836468301 2316 68-69 -0.8501393835279814 2316 70-71 -0.8838049857437973 2316 72-73 -0.8897925962275757 2316 74-75 -0.8675174292074104 2316 76-77 -0.9740435822786893 2316 78-79 -0.9994342347345402 2316 80-81 -0.9276248844769448 2316 82-83 -1.01956445840543 2316 84-85 -1.1409244473392022 2316 86-87 -1.9349938551169785 2316 88-89 -1.7195639341947775 2316 90-91 -1.7831018056798307 2316 92-93 -1.4131048394747445 2316 94-95 -1.5847867067666854 2316 96-97 -1.7197827951309286 2316 98-99 -1.7196481443866816 2316 100-101 -1.8641546410647543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5062.0 3 737.0 4 662.0 5 780.0 6 872.0 7 986.0 8 1129.0 9 1447.0 10 1819.0 11 2404.0 12 3378.0 13 4693.0 14 5465.0 15 7186.0 16 9610.0 17 13623.0 18 11745.0 19 8053.0 20 7548.0 21 7725.0 22 8564.0 23 9463.0 24 10728.0 25 12024.0 26 14183.0 27 16687.0 28 20191.0 29 24506.0 30 31021.0 31 40210.0 32 52893.0 33 72815.0 34 105952.0 35 159021.0 36 226341.0 37 280132.0 38 165216.0 39 12175.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20080372350492 18.327660744700985 14.922333341218774 23.549202190575322 2 15.11993060266033 19.219634599953007 40.70347563039119 24.95695916699548 3 18.92320674856055 23.494564382071623 29.812620446672423 27.769608422695402 4 12.796305532465132 15.809513253902283 36.07950494135033 35.314676272282256 5 14.030423560932464 36.53132686892391 33.80344757008303 15.63480200006059 6 32.84309056014981 35.21580946178152 16.78536907032573 15.155730907742942 7 28.948357424538546 32.61398129162922 20.006910977600604 18.43075030623162 8 27.031138087115757 34.17015557967644 19.185624940005464 19.613081393202343 9 26.418963272762447 16.469255641923645 20.039932628457464 37.07184845685644 10-11 25.14921796715026 26.147831936595562 26.56866241774828 22.134287678505892 12-13 25.967272893762072 23.433318465655663 27.615332809518467 22.984075831063798 14-15 22.9458365500739 24.71597611507827 25.94950612648316 26.388681208364673 16-17 22.192724905961153 28.17754209459003 26.6117846762365 23.01794832321232 18-19 22.206730153386246 26.136867444617085 29.599104880813815 22.05729752118285 20-21 22.64813803083102 25.1817671726419 30.002104128521644 22.167990668005434 22-23 22.367855623944145 25.002510425650055 30.071687419518707 22.557946530887097 24-25 22.177966711767173 25.420071508602625 29.970152449548042 22.43180933008216 26-27 21.820852089871895 25.670648166163396 29.85290246550723 22.65559727845748 28-29 21.966233911042337 25.86948952853968 29.812472562791285 22.351803997626693 30-31 21.859672752889885 25.666626790901827 29.774975151490985 22.698725304717303 32-33 21.677736877277578 25.731796918555496 29.75033875213682 22.840127452030114 34-35 22.29570418505049 25.643707655670216 29.68461666266684 22.375971496612454 36-37 22.227829465320042 25.56569371359548 29.477613317788926 22.728863503295557 38-39 21.986747632846676 25.76422599162037 29.579504974252018 22.66952140128094 40-41 22.222238627743746 25.656828890615568 29.669504616821364 22.451427864819323 42-43 21.929883821419224 25.69624641037312 29.471358109188152 22.9025116590195 44-45 21.991935918535205 25.930651262070835 29.4334912629273 22.64392155646666 46-47 22.247930222899072 26.185758456781578 28.865974433522545 22.70033688679681 48-49 22.315331175122285 26.285622297313267 28.52209557172901 22.87695095583544 50-51 22.305271773863353 26.610663907017646 28.479998759487064 22.604065559631938 52-53 22.602696985507052 26.352674369702132 28.304759879229756 22.73986876556106 54-55 22.356918094449068 26.577667707436873 28.28070595158849 22.784708246525568 56-57 22.241780745062837 26.810354755267884 28.228940281583668 22.718924218085608 58-59 22.307093861710033 26.31468617668703 28.302520462565813 23.07569949903712 60-61 22.047640825691627 26.534698929220156 28.525362411120547 22.892297833967664 62-63 22.14922001731039 26.563702431021408 28.718975549531162 22.56810200213704 64-65 21.978233715151354 26.513725368603513 28.591171233422806 22.916869682822323 66-67 22.03311128967783 26.645054105839044 28.391234843001346 22.930599761481783 68-69 22.169599081057477 26.42919443776966 28.66295265652124 22.738253824651622 70-71 22.10245980303858 26.558822660898578 28.404007146126474 22.934710389936363 72-73 22.10618214571805 26.583558822117958 28.278471573647156 23.03178745851683 74-75 22.024240652264922 27.006007742065048 28.052360458617308 22.917391147052722 76-77 22.26827316234722 26.74735569078379 28.085495024431157 22.89887612243783 78-79 22.256167677093575 26.791626629200334 28.131717388583866 22.820488305122225 80-81 22.314738148878373 26.969766659788263 27.964612288909073 22.750882902424287 82-83 22.225335102138143 26.902443327744912 27.966789877652747 22.905431692464198 84-85 22.194264918115078 26.883796123707686 27.93532622938113 22.986612728796104 86-87 22.372623102579208 26.965374331353413 27.817174343460294 22.844828222607084 88-89 22.332704694342286 26.904235358303925 27.60275183672957 23.16030811062422 90-91 22.221496276822915 26.823744025254044 27.853934371828988 23.100825326094057 92-93 22.345393568809484 26.66277626812597 27.903200683292766 23.088629479771775 94-95 22.207904525282217 26.518987271686356 27.951868422538816 23.321239780492604 96-97 22.462832157312953 26.398129195513466 27.800494279243775 23.338544367929803 98-99 22.21582792237755 26.589012222177104 27.615475764209062 23.579684091236285 100-101 22.65421917046793 25.819524346102302 28.22414646452157 23.302110018908195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2503.0 1 1545.5 2 807.5 3 951.0 4 664.5 5 591.0 6 1220.5 7 2738.0 8 4400.5 9 4755.0 10 3583.0 11 2478.0 12 2296.5 13 2312.5 14 2328.5 15 2346.0 16 2310.5 17 2289.0 18 2334.5 19 2415.0 20 2555.5 21 2916.5 22 3501.0 23 4205.0 24 4946.5 25 5968.0 26 7288.5 27 8670.5 28 9947.0 29 11547.0 30 13540.0 31 15859.5 32 18900.0 33 20372.0 34 21310.0 35 23200.0 36 25738.0 37 28931.0 38 31665.5 39 33538.0 40 35313.0 41 38279.5 42 40671.5 43 43004.0 44 45887.5 45 48210.5 46 48837.5 47 49608.5 48 51421.5 49 51529.0 50 50663.5 51 49640.5 52 48521.5 53 48820.5 54 49092.5 55 46233.5 56 42530.5 57 38790.5 58 35709.5 59 32299.5 60 26944.5 61 22284.5 62 18224.5 63 13595.0 64 10103.5 65 8125.0 66 6151.0 67 4542.0 68 3376.5 69 2533.5 70 1903.0 71 1392.5 72 1067.0 73 892.0 74 700.0 75 544.5 76 429.5 77 291.5 78 194.0 79 154.0 80 97.5 81 50.5 82 37.5 83 29.0 84 19.0 85 15.5 86 13.0 87 10.0 88 11.5 89 9.5 90 5.5 91 3.5 92 3.0 93 3.5 94 3.5 95 2.0 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3201065842917863 2 0.2713242272933603 3 0.2764824825650972 4 0.270661023044137 5 0.271840052820534 6 0.20787768745099658 7 0.19756117690752278 8 0.20323525770643336 9 0.2038984619556567 10-11 0.20787768745099658 12-13 0.21222535975146053 14-15 0.20666181299408715 16-17 0.1980401577541841 18-19 0.19096597909580207 20-21 0.18923427911171897 22-23 0.19866651732289498 24-25 0.1964189918116382 26-27 0.2020930726105488 28-29 0.20776715340945937 30-31 0.20942516403251762 32-33 0.20728817256279808 34-35 0.20802506617304622 36-37 0.1952031173547288 38-39 0.18960272591684302 40-41 0.183523353632296 42-43 0.19339772800962088 44-45 0.19505573863267917 46-47 0.20157724708337507 48-49 0.20927778531046803 50-51 0.20408268535821872 52-53 0.21417812781861803 54-55 0.20356685983104503 56-57 0.17862301112414594 58-59 0.16631688783300222 60-61 0.18234432385589897 62-63 0.17468063030931846 64-65 0.1824548578974362 66-67 0.1793967494149065 68-69 0.18035471110822904 70-71 0.1818653430092377 72-73 0.19033961952709116 74-75 0.19372933013423252 76-77 0.19380301949525736 78-79 0.1906712216517028 80-81 0.18912374507018176 82-83 0.181349517482064 84-85 0.17707553454262487 86-87 0.18024417706669182 88-89 0.18676568551738773 90-91 0.1836707323543456 92-93 0.17913883665131963 94-95 0.18197587705077492 96-97 0.18794471529378476 98-99 0.18739204508609864 100-101 0.17276470692267334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1357048.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.772476673944645 #Duplication Level Percentage of deduplicated Percentage of total 1 72.40449517557435 36.76155542390587 2 14.35061472067107 14.572325023240772 3 5.233374588384102 7.971341676442393 4 2.541368519882507 5.161262955825271 5 1.457359886697518 3.699688542644617 6 0.9149271021356481 2.7871868970925178 7 0.5917271769911924 2.10304180037871 8 0.44633214651130976 1.8129110798061694 9 0.2989812602855053 1.3662017157413153 >10 1.6331522972354005 14.504459118950711 >50 0.08726334863060983 3.021512229890252 >100 0.03706104302750336 3.3697478829103877 >500 0.002180043895659266 0.7944319526208033 >1k 7.266812985530887E-4 0.5312938936345702 >5k 4.360087791318532E-4 1.543039806915625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9069 0.6682888151340262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6814 0.5021193060230736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5028 0.3705101072327582 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1892 0.1394202710589456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1618 0.11922938613814693 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1522 0.1121552074797649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.368936102481268E-5 14-15 0.0 0.0 0.0 0.0 7.368936102481268E-5 16-17 0.0 0.0 0.0 0.0 7.368936102481268E-5 18-19 0.0 0.0 0.0 0.0 7.368936102481268E-5 20-21 0.0 0.0 0.0 0.0 7.368936102481268E-5 22-23 0.0 0.0 0.0 7.368936102481268E-5 7.368936102481268E-5 24-25 0.0 0.0 0.0 1.4737872204962537E-4 7.368936102481268E-5 26-27 0.0 0.0 0.0 3.684468051240634E-4 7.368936102481268E-5 28-29 0.0 0.0 0.0 9.211170128101586E-4 7.368936102481268E-5 30-31 0.0 0.0 0.0 0.002542282955356037 7.368936102481268E-5 32-33 0.0 0.0 0.0 0.004937187188662449 7.368936102481268E-5 34-35 0.0 0.0 0.0 0.008290053115291428 7.368936102481268E-5 36-37 0.0 0.0 0.0 0.01307986158190425 7.368936102481268E-5 38-39 0.0 0.0 0.0 0.020338263642848298 7.368936102481268E-5 40-41 0.0 0.0 0.0 0.029807346534536726 7.368936102481268E-5 42-43 0.0 0.0 0.0 0.03975541027288644 7.368936102481268E-5 44-45 0.0 0.0 0.0 0.05180362080044332 7.368936102481268E-5 46-47 0.0 0.0 0.0 0.06831003767000135 7.368936102481268E-5 48-49 0.0 0.0 0.0 0.08459538645648496 7.368936102481268E-5 50-51 0.0 0.0 0.0 0.10662850540290394 7.368936102481268E-5 52-53 0.0 0.0 0.0 0.12803526478061203 1.4737872204962537E-4 54-55 0.0 0.0 0.0 0.15426867730544536 1.4737872204962537E-4 56-57 0.0 0.0 0.0 0.18746573444712347 1.842234025620317E-4 58-59 0.0 0.0 0.0 0.22206288944827302 2.2106808307443805E-4 60-61 0.0 0.0 0.0 0.26207621248474633 2.2106808307443805E-4 62-63 0.0 0.0 0.0 0.30547924612836097 2.9475744409925074E-4 64-65 0.0 0.0 0.0 0.3583513626636641 2.9475744409925074E-4 66-67 0.0 0.0 0.0 0.41693440467839016 2.9475744409925074E-4 68-69 0.0 0.0 0.0 0.4909922125083269 2.9475744409925074E-4 70-71 0.0 0.0 0.0 0.5744822585494397 3.684468051240634E-4 72-73 0.0 0.0 0.0 0.6615830832807683 4.0529148563646974E-4 74-75 0.0 0.0 0.0 0.7517420164946266 4.789808466612824E-4 76-77 0.0 0.0 0.0 0.8589968814662414 5.158255271736887E-4 78-79 0.0 0.0 0.0 0.9837161250007369 5.158255271736887E-4 80-81 0.0 0.0 0.0 1.1193045492863922 5.158255271736887E-4 82-83 0.0 0.0 0.0 1.271509924483143 5.158255271736887E-4 84-85 0.0 0.0 0.0 1.4251522422198772 5.158255271736887E-4 86-87 0.0 0.0 0.0 1.5964800066025666 5.158255271736887E-4 88-89 0.0 0.0 0.0 1.7937095813854778 5.526702076860951E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3050 0.0 52.703735 1 GTATCAA 5555 0.0 42.12167 1 ATCAACG 7315 0.0 31.714172 3 TATCAAC 7435 0.0 31.266249 2 TCAACGC 7455 0.0 31.184675 4 CAACGCA 7620 0.0 30.506035 5 AACGCAG 7815 0.0 29.789152 6 TGGTATC 1575 0.0 29.277843 2 GTGGTAT 1625 0.0 29.266525 1 TACGCGC 35 0.008410081 27.131907 58-59 ACGCAGA 8970 0.0 25.741564 7 GTACATG 5200 0.0 25.699669 1 CGCAGAG 9005 0.0 25.53599 8 TACATGG 5375 0.0 24.233713 2 GGACCGT 140 1.7766954E-5 23.755331 6 ATACCGT 200 2.0498192E-8 23.755331 6 GCAGAGT 9585 0.0 23.594234 9 ACATGGG 5345 0.0 23.480326 3 GAGTACT 6170 0.0 21.78532 12-13 AGAGTAC 8905 0.0 21.576422 10-11 >>END_MODULE