FastQCFastQC Report
Thu 26 May 2016
SRR937536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357048
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84790.6248120921293867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77090.568071284140281No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54830.40403876649904796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21710.15997960278486834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.14089405827944185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.12048210527556874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40950.053.9593051
GTATCAA66750.036.449031
ACGGTAC650.005833026529.2424621
TATCAAC88400.027.4067272
ATCAACG87750.027.3931923
TCAACGC89750.026.8356884
CAACGCA91850.026.2221365
AACGCAG93200.025.83856
GTAAGGT4800.025.7318734
TAAGGTG6100.022.584345
GAGTACT74500.022.34719512-13
GTGGTAT21050.022.1228621
TGGTATC19400.022.0383992
ACGCAGA108550.021.9667177
CGCAGAG109200.021.7489688
GTACTTT77600.021.33125114-15
AGGTAAG5300.020.6153762
AGTACTT75900.020.46431512-13
GTGTAGG4000.020.1955781
GCAGAGT117350.019.8337219
Produced by FastQC (version 0.11.5)