##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937536_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1357048 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.346331890986907 31.0 31.0 34.0 30.0 34.0 2 31.64703238205281 31.0 31.0 34.0 30.0 34.0 3 31.68768753942381 31.0 31.0 34.0 30.0 34.0 4 35.529742499896834 37.0 35.0 37.0 33.0 37.0 5 35.23827086440568 37.0 35.0 37.0 33.0 37.0 6 35.41867789496024 37.0 35.0 37.0 33.0 37.0 7 35.35514071720381 37.0 35.0 37.0 33.0 37.0 8 35.342648159829274 37.0 35.0 37.0 33.0 37.0 9 36.87805958226975 39.0 37.0 39.0 33.0 39.0 10-11 36.753079478397225 38.5 36.0 39.0 32.0 39.0 12-13 36.637757839074226 38.0 35.0 39.0 32.0 39.0 14-15 37.54622017791559 39.0 36.0 40.0 32.0 41.0 16-17 37.46322311369974 39.0 36.0 40.0 32.0 41.0 18-19 37.412835802418186 39.0 36.0 40.0 32.0 41.0 20-21 37.28432524125897 39.0 36.0 40.0 32.0 41.0 22-23 37.06839809645643 39.0 36.0 40.0 31.0 41.0 24-25 36.949041227723704 39.0 36.0 40.0 31.0 41.0 26-27 36.763973713531136 38.5 35.5 40.0 30.5 41.0 28-29 36.57408838891476 38.0 35.0 40.0 30.0 41.0 30-31 36.62264488802165 38.0 35.0 40.0 30.0 41.0 32-33 36.4706911619928 38.0 35.0 40.0 30.0 41.0 34-35 36.23978665456196 38.0 35.0 40.0 30.0 41.0 36-37 35.96954160796081 38.0 35.0 40.0 30.0 41.0 38-39 35.743580551314324 38.0 35.0 40.0 29.0 41.0 40-41 35.45403662950758 38.0 34.0 40.0 28.0 41.0 42-43 35.15243012774788 38.0 34.0 40.0 27.0 41.0 44-45 34.70828555806427 38.0 33.0 40.0 25.5 41.0 46-47 34.219832312490055 37.5 33.0 40.0 24.0 41.0 48-49 33.727818028544306 37.0 33.0 40.0 23.0 41.0 50-51 33.35068287930862 37.0 32.0 39.5 22.0 40.5 52-53 32.98615045304219 36.5 31.5 39.0 20.5 40.0 54-55 32.62856619662679 36.0 31.0 39.0 19.5 40.0 56-57 32.170905155897216 36.0 31.0 39.0 18.0 40.0 58-59 31.755865673137578 35.0 30.0 38.5 15.0 40.0 60-61 31.21870375992596 35.0 29.0 38.0 12.0 40.0 62-63 30.65827922077922 34.0 28.5 38.0 8.0 40.0 64-65 30.065831864458737 34.0 28.0 37.5 7.5 39.5 66-67 29.452565421414718 34.0 27.5 37.0 6.5 39.0 68-69 28.808314812740598 33.0 26.0 36.0 2.0 39.0 70-71 28.25522604948388 32.5 26.0 36.0 2.0 38.5 72-73 27.66636478591767 32.0 25.5 35.0 2.0 38.0 74-75 27.014410691442013 31.5 24.5 35.0 2.0 37.0 76-77 26.188463856842205 30.5 23.5 34.0 2.0 36.0 78-79 26.701771418549676 31.5 25.0 34.5 2.0 36.0 80-81 26.381690625534247 31.5 24.0 34.5 2.0 36.0 82-83 25.849757709380953 31.0 22.5 34.0 2.0 35.5 84-85 25.205106230582853 31.0 20.0 34.0 2.0 35.0 86-87 24.487854887962698 30.5 18.5 34.0 2.0 35.0 88-89 23.719918160595647 30.0 13.0 34.0 2.0 35.0 90-91 22.79047977669176 29.0 4.5 34.0 2.0 35.0 92-93 21.886410429107887 29.0 2.0 33.5 2.0 35.0 94-95 20.944949994399607 28.5 2.0 33.0 2.0 34.5 96-97 19.858789077468153 26.5 2.0 33.0 2.0 34.0 98-99 18.49710290277131 25.0 2.0 32.5 2.0 34.0 100-101 16.2037072380638 18.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12120750416087489 1101 2 0.07872337869214618 1101 3 0.2152648985914425 1101 4 0.11639485340388234 1101 5 -0.06246390482566255 1101 6 -0.0015438938496146193 1101 7 -0.01619030952968359 1101 8 0.07171612665031546 1101 9 0.20715160736490645 1101 10-11 0.13541837991537875 1101 12-13 0.2019413024033767 1101 14-15 0.24243782092219135 1101 16-17 -0.03712935669381778 1101 18-19 0.11550041009925849 1101 20-21 0.1160063531403992 1101 22-23 -0.005821636382393081 1101 24-25 0.12817165363457406 1101 26-27 0.037919464313048934 1101 28-29 0.05526619119789444 1101 30-31 0.1827635463047912 1101 32-33 0.21481310593947 1101 34-35 0.040374933129093904 1101 36-37 0.20839021121395263 1101 38-39 0.37100605279306365 1101 40-41 0.16989065988841645 1101 42-43 0.244483731879221 1101 44-45 0.23969113832308153 1101 46-47 0.14973772469192426 1101 48-49 0.34536747666940215 1101 50-51 0.42973492635454846 1101 52-53 0.4587187067241061 1101 54-55 0.49767607888234267 1101 56-57 0.5780510823369198 1101 58-59 0.586024327424898 1101 60-61 0.5867467488498299 1101 62-63 0.7576545523119869 1101 64-65 0.548106605411057 1101 66-67 0.6175534398725162 1101 68-69 0.5403031340576021 1101 70-71 0.550970339893972 1101 72-73 0.39297292876583256 1101 74-75 0.5102224432211706 1101 76-77 0.46807176395855876 1101 78-79 0.7282671145482702 1101 80-81 0.7776594127491911 1101 82-83 0.725440485672884 1101 84-85 1.0606566535702413 1101 86-87 1.398826480074689 1101 88-89 1.5187103685756504 1101 90-91 1.2628784437895568 1101 92-93 1.4712756647464182 1101 94-95 1.2641680146917444 1101 96-97 1.6740409184275045 1101 98-99 1.9450770780493123 1101 100-101 1.9391725170319845 1102 1 0.13389857115418735 1102 2 0.22321975134182281 1102 3 0.14529058314580823 1102 4 0.14340726195771936 1102 5 -0.0924607027275286 1102 6 0.05175240357616673 1102 7 0.08702525774995706 1102 8 0.081396543702418 1102 9 0.13719550491131827 1102 10-11 0.21201544753895263 1102 12-13 0.09695420397867593 1102 14-15 0.15453006167930994 1102 16-17 0.28023732420438563 1102 18-19 0.2879073459454773 1102 20-21 0.2213998554225256 1102 22-23 0.20108917756045486 1102 24-25 0.2659199009517508 1102 26-27 0.016447829142151704 1102 28-29 0.043986207530295474 1102 30-31 0.2595757813692927 1102 32-33 0.2478154710870939 1102 34-35 0.3543802580997024 1102 36-37 0.29125551300746366 1102 38-39 0.14360943481862165 1102 40-41 0.17220307454149264 1102 42-43 0.2350235649634982 1102 44-45 0.36516213476858184 1102 46-47 0.41366325134388404 1102 48-49 0.48404310412815477 1102 50-51 0.5395010291808617 1102 52-53 0.4126239534211962 1102 54-55 0.3012428180621285 1102 56-57 0.5138011526475452 1102 58-59 0.6544422791663997 1102 60-61 0.7401219551225537 1102 62-63 0.7975875962705246 1102 64-65 0.7497553471050828 1102 66-67 0.8106893020162644 1102 68-69 0.6545699637093669 1102 70-71 0.7188718897772404 1102 72-73 0.8118181657719248 1102 74-75 0.8012912578349365 1102 76-77 0.7114172449340295 1102 78-79 1.013578082327367 1102 80-81 0.7792630031652585 1102 82-83 0.6327456744272482 1102 84-85 0.8793740607856364 1102 86-87 1.1563617878657837 1102 88-89 1.1522666628162774 1102 90-91 1.2216514302797563 1102 92-93 1.419330517195771 1102 94-95 1.1486330525239445 1102 96-97 1.7267783311322091 1102 98-99 2.049737401512054 1102 100-101 2.2126799835116095 1103 1 0.1608463708292618 1103 2 0.2710123627707972 1103 3 0.21914803882144795 1103 4 0.18687692360919783 1103 5 -0.10951940160440898 1103 6 0.04088851998501042 1103 7 0.06928844910405729 1103 8 0.1090153895030852 1103 9 0.15719267945394932 1103 10-11 0.1736210136899743 1103 12-13 0.15125243100417407 1103 14-15 0.3567339184286169 1103 16-17 0.320764978368409 1103 18-19 0.2677123893868796 1103 20-21 0.19250602198324174 1103 22-23 0.30457508559181434 1103 24-25 0.3247282643055698 1103 26-27 0.2723530350473595 1103 28-29 0.22820602811374613 1103 30-31 0.31643104731546856 1103 32-33 0.17009420245673113 1103 34-35 0.3407580217501902 1103 36-37 0.32230375769206887 1103 38-39 0.37385948779594713 1103 40-41 0.33868608647905063 1103 42-43 0.36529480887111276 1103 44-45 0.39862614550531816 1103 46-47 0.48866148543302756 1103 48-49 0.6412268294936965 1103 50-51 0.60275948789387 1103 52-53 0.5604236284936022 1103 54-55 0.4084512661796609 1103 56-57 0.7147194262930938 1103 58-59 0.5156593449289169 1103 60-61 0.34547972866214494 1103 62-63 0.5096169810271824 1103 64-65 0.8218645510324727 1103 66-67 0.6980453802814317 1103 68-69 0.5533762079701567 1103 70-71 0.8172472517889666 1103 72-73 0.995903777130259 1103 74-75 0.8679473290364612 1103 76-77 0.8982046999033031 1103 78-79 1.0509306287708213 1103 80-81 0.9982500766977864 1103 82-83 1.0980490579124513 1103 84-85 1.2759093436845568 1103 86-87 1.6298766568351972 1103 88-89 1.8621663590796125 1103 90-91 2.0792059969252286 1103 92-93 2.0583218289143552 1103 94-95 1.9137068675977567 1103 96-97 2.077149172412142 1103 98-99 2.4715640597023487 1103 100-101 2.6456954528907133 1104 1 0.024256124781501853 1104 2 0.1586267167317672 1104 3 0.07241060278972355 1104 4 0.0013777052727306227 1104 5 -0.1218346587588357 1104 6 -0.03019216761031629 1104 7 -0.007467216417815337 1104 8 0.0838555597345163 1104 9 0.11694388999728744 1104 10-11 -0.017385994983861508 1104 12-13 0.1320306702077616 1104 14-15 0.12334821307939592 1104 16-17 0.11581862051758662 1104 18-19 0.06268254964749076 1104 20-21 0.26871883141736674 1104 22-23 0.11969929567522541 1104 24-25 0.12006637154959066 1104 26-27 0.06744629192650109 1104 28-29 0.06802487465366625 1104 30-31 0.18635216476419458 1104 32-33 0.11040946408740382 1104 34-35 0.2469613079822821 1104 36-37 0.20606888039142746 1104 38-39 0.016610271977356206 1104 40-41 0.004955598691914531 1104 42-43 0.263392070866594 1104 44-45 0.2816524646814287 1104 46-47 0.3244601083175809 1104 48-49 0.1887175049248455 1104 50-51 0.3317513153974545 1104 52-53 0.21248835045750525 1104 54-55 0.1287701535652772 1104 56-57 0.44432998216384334 1104 58-59 0.28639804773675337 1104 60-61 0.08293724634049582 1104 62-63 0.24239749863727056 1104 64-65 0.3290124359528015 1104 66-67 0.3080122896722486 1104 68-69 0.2672969807483483 1104 70-71 0.4894670307298199 1104 72-73 0.592285519887426 1104 74-75 0.6633395987926285 1104 76-77 0.5733022539734947 1104 78-79 0.6446319986123754 1104 80-81 0.5430519862620571 1104 82-83 0.7926795834119176 1104 84-85 0.6384697309688967 1104 86-87 0.9942411929232087 1104 88-89 1.2915007309007827 1104 90-91 1.301637781815007 1104 92-93 1.7034698637887438 1104 94-95 1.7175519583367524 1104 96-97 2.0083771059444437 1104 98-99 2.1809980726409215 1104 100-101 2.47688556218932 1105 1 0.06525610583110009 1105 2 0.1583666751251087 1105 3 0.06564846821661163 1105 4 0.06713164695602813 1105 5 -0.17219192644798653 1105 6 0.01647740478442472 1105 7 -0.03156344023467739 1105 8 0.030988153581112954 1105 9 0.08847565086850295 1105 10-11 0.05197681359182127 1105 12-13 -0.07970926081277696 1105 14-15 0.1153712788751875 1105 16-17 -0.12628906408037466 1105 18-19 0.024174019861668228 1105 20-21 0.12202693966205658 1105 22-23 0.1347922368616068 1105 24-25 0.16334737545757605 1105 26-27 0.11254863988111907 1105 28-29 0.08664364313030859 1105 30-31 0.14081264159838014 1105 32-33 0.250754550880238 1105 34-35 0.04345400997249982 1105 36-37 0.07410645061318633 1105 38-39 0.0847024825205196 1105 40-41 0.13888544852051865 1105 42-43 0.33427867588129345 1105 44-45 0.2525263571357925 1105 46-47 0.3611713768573992 1105 48-49 0.21289940034389332 1105 50-51 0.4700158061370203 1105 52-53 0.4074941566496548 1105 54-55 0.37909520557360565 1105 56-57 0.5299207819549778 1105 58-59 0.5894827254957242 1105 60-61 0.4724587803122482 1105 62-63 0.7012338071834812 1105 64-65 0.46621570426520265 1105 66-67 0.5837503551311443 1105 68-69 0.38151117607223384 1105 70-71 0.6777678167503218 1105 72-73 0.6368734434288754 1105 74-75 0.4863150906608027 1105 76-77 0.5333008537494557 1105 78-79 0.7698730898185495 1105 80-81 0.8502766685270124 1105 82-83 1.0344820876020577 1105 84-85 1.2070776819252629 1105 86-87 1.1733498503083943 1105 88-89 1.0656045875178428 1105 90-91 1.1397204276699213 1105 92-93 1.4092672650571565 1105 94-95 1.517045798403796 1105 96-97 1.7132647563895667 1105 98-99 2.125347721216272 1105 100-101 2.3746140671711693 1106 1 -0.2042626237618066 1106 2 0.03284495249183195 1106 3 -0.0525741301118785 1106 4 0.048359929315310524 1106 5 -0.22251663013948075 1106 6 -0.22684878918656182 1106 7 -0.2931533587560793 1106 8 -0.27663349016970074 1106 9 -0.31648158784581426 1106 10-11 -0.16059629622455418 1106 12-13 -0.31459022567513273 1106 14-15 -0.2192961603903001 1106 16-17 -0.11813808404063053 1106 18-19 -0.07208911057397671 1106 20-21 -0.217535861763821 1106 22-23 -0.14537032092729163 1106 24-25 -0.0725082309388938 1106 26-27 -0.38117524278371206 1106 28-29 -0.32613007403111993 1106 30-31 -0.366985270762207 1106 32-33 -0.3548699436229512 1106 34-35 -0.7389864038109621 1106 36-37 -0.44932395058902586 1106 38-39 -0.5387320375445839 1106 40-41 -0.47805905417047256 1106 42-43 -0.493942898972179 1106 44-45 -0.20338605212440086 1106 46-47 -0.14440266157534154 1106 48-49 -0.15552024812987497 1106 50-51 -0.3922915434121492 1106 52-53 -0.30539130026953387 1106 54-55 -0.43597190692876353 1106 56-57 -0.47915787834118717 1106 58-59 -0.5211968931225393 1106 60-61 -0.4459250153322394 1106 62-63 -0.4310980808034195 1106 64-65 -0.41478379398240506 1106 66-67 -0.3136709246619631 1106 68-69 -0.3843051014242995 1106 70-71 -0.23024284111945192 1106 72-73 0.08129594140688567 1106 74-75 0.08082130764748996 1106 76-77 0.1810965756238616 1106 78-79 0.49782344379112686 1106 80-81 0.46169659034350374 1106 82-83 0.46445595778548565 1106 84-85 0.8966591118446807 1106 86-87 0.6568918726950379 1106 88-89 0.9830538381344205 1106 90-91 1.1029291529193017 1106 92-93 1.4686640763083965 1106 94-95 1.4330380012715231 1106 96-97 1.633129314448059 1106 98-99 1.90264532903063 1106 100-101 2.4206525011545352 1107 1 0.023890830732359092 1107 2 0.13531548257130055 1107 3 0.0031312458413026434 1107 4 0.023257645853796305 1107 5 -0.27197358500099966 1107 6 -0.141719680424238 1107 7 0.10860468842029292 1107 8 -0.06334453678354635 1107 9 -0.01311531389069387 1107 10-11 0.04371055184233086 1107 12-13 0.02885772274559173 1107 14-15 0.11771017312214838 1107 16-17 0.09086021181442305 1107 18-19 0.1579067209424707 1107 20-21 0.09651103384165793 1107 22-23 0.07686382070777853 1107 24-25 0.05472537597807303 1107 26-27 0.1619208843010682 1107 28-29 0.04214485252377642 1107 30-31 0.11782029216021073 1107 32-33 0.08440362776092059 1107 34-35 0.4177038798478847 1107 36-37 0.05147911985172726 1107 38-39 0.19277193079216204 1107 40-41 0.28787430373009215 1107 42-43 0.5486608901429548 1107 44-45 0.40389429213472283 1107 46-47 0.5525545440904835 1107 48-49 0.2432763215955447 1107 50-51 0.42347042326448303 1107 52-53 0.388885858526514 1107 54-55 0.31206859126296393 1107 56-57 0.5591691592245027 1107 58-59 0.6056043039838208 1107 60-61 0.7598131555610159 1107 62-63 0.7613542489948166 1107 64-65 0.7958529244972858 1107 66-67 1.0505197819704932 1107 68-69 1.095536026046208 1107 70-71 1.252417724132215 1107 72-73 1.1067907377554427 1107 74-75 1.37784330856595 1107 76-77 1.3654888991246779 1107 78-79 1.4312123017226526 1107 80-81 1.4159848143278104 1107 82-83 1.6021301426218102 1107 84-85 1.8106994517730932 1107 86-87 1.947782660081515 1107 88-89 2.4061368737307554 1107 90-91 2.192593598270946 1107 92-93 2.4554701140207555 1107 94-95 2.58989118357265 1107 96-97 3.0822680209864863 1107 98-99 3.3498686521430656 1107 100-101 3.6028741671374895 1108 1 -0.0446144104276982 1108 2 0.01754926535865664 1108 3 0.097788930838135 1108 4 -0.1410668947804794 1108 5 -0.314237825431114 1108 6 -0.08023451833548023 1108 7 0.0049245751860382825 1108 8 -0.04090814768159845 1108 9 -0.11782253887336935 1108 10-11 -0.0365173617350294 1108 12-13 -0.029535358700812253 1108 14-15 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60-61 0.6631764463588645 2316 62-63 0.5673376387478406 2316 64-65 0.6689696981376194 2316 66-67 0.9849350671711186 2316 68-69 0.5208618754558252 2316 70-71 0.5840651186068335 2316 72-73 0.2959478771743633 2316 74-75 0.7339083949975276 2316 76-77 0.44054704224564745 2316 78-79 0.3909293687695623 2316 80-81 0.5834702619179701 2316 82-83 0.2718536317170219 2316 84-85 0.03323310100831023 2316 86-87 0.2885612155197599 2316 88-89 0.19718809410134597 2316 90-91 0.19943531715455265 2316 92-93 -0.10848973789721228 2316 94-95 -0.150905997015105 2316 96-97 -1.0922453969824275 2316 98-99 -1.013958525820236 2316 100-101 -0.9923565851613247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 16.0 4 28.0 5 70.0 6 170.0 7 401.0 8 889.0 9 1523.0 10 2393.0 11 3692.0 12 5285.0 13 7470.0 14 9566.0 15 12937.0 16 16895.0 17 21616.0 18 20175.0 19 16437.0 20 16020.0 21 16966.0 22 18726.0 23 20420.0 24 23653.0 25 27902.0 26 33503.0 27 39563.0 28 47398.0 29 55634.0 30 66009.0 31 78131.0 32 92544.0 33 111164.0 34 132987.0 35 154899.0 36 156621.0 37 113951.0 38 30741.0 39 652.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08777074298738 21.71148120546281 16.52812085588066 20.672627195669143 2 17.92869522669795 22.485645312472364 36.72265093054925 22.863008530280432 3 21.174637890479925 24.91776267309631 30.521543821589216 23.386055614834554 4 15.563782563328637 18.589025590841295 34.736796340291576 31.11039550553849 5 14.11053886813812 37.85343046068671 33.45601999935144 14.580010671823732 6 32.79987148575437 36.92588618825569 15.587657916300676 14.686584409689265 7 28.62684297091923 33.75672783866157 19.52952290560098 18.086906284818223 8 27.28952844704093 35.59476157070347 18.348429827095284 18.767280155160318 9 26.52242219877263 16.72269514416587 19.589358666753128 37.16552399030837 10-11 25.766774646143688 27.147492203665603 25.835268907216253 21.250464242974456 12-13 26.449408328064365 24.23950001547529 27.151249592852476 22.159842063607865 14-15 23.150249659555154 25.592167705195397 25.650861281251657 25.6067213539978 16-17 22.214763221345155 28.963640195483137 26.451790946230346 22.36980563694136 18-19 22.29950598652369 26.71364609063202 29.6569833933656 21.32986452947869 20-21 22.689118357728212 25.747671230252156 30.211628631874948 21.35158178014468 22-23 22.656229274806122 25.62219795378779 30.17293470219094 21.548638069215148 24-25 22.601675262574968 25.995014152114788 29.975844502855846 21.427466082454398 26-27 22.190928126624655 26.290098476918335 29.910498640954415 21.608474755502595 28-29 22.222979607702978 26.311313681022398 30.01379874466427 21.451907966610353 30-31 22.1351989562612 26.286168213348937 29.9419805319575 21.636652298432367 32-33 22.144021434761335 26.196383620918347 29.802630415431143 21.85696452888918 34-35 22.581183569041034 26.204342071909025 29.75532184565321 21.459152513396727 36-37 22.697539070099214 26.03655139685553 29.571540579257327 21.69436895378793 38-39 22.392759872900587 26.24700821194239 29.661920580554263 21.698311334602753 40-41 22.523263731275534 26.17947191256315 29.70116016529997 21.59610419086134 42-43 22.414077092666037 26.28153374660393 29.369750670554453 21.93463849017558 44-45 22.472823363654047 26.46380231207739 29.25003389710607 21.81334042716249 46-47 22.753579829158586 26.8721887508769 28.580418673473602 21.79381274649091 48-49 22.889978836415516 26.963600403228185 28.207845264132146 21.938575496224157 50-51 22.832132688011036 27.29214441935731 28.07490965684338 21.80081323578827 52-53 23.23149218008501 27.129733067658623 27.840982780270117 21.797791971986253 54-55 23.012192641675167 27.285401842823543 27.8418302079219 21.860575307579396 56-57 23.00673962895933 27.45860868591236 27.738701947167677 21.795949737960633 58-59 22.96112591448497 27.07774522345562 27.805280284853595 22.155848577205816 60-61 22.765480660964094 27.164367067340283 28.07833621213104 21.991816059564584 62-63 22.74823735048429 27.250694153780852 28.25688553389416 21.744182961840703 64-65 22.588552505143518 27.26292658771097 28.104790692738945 22.043730214406565 66-67 22.567256279807346 27.435875518036205 27.936889483643913 22.059978718512536 68-69 22.815736805183015 27.141560210103105 27.95888575790982 22.08381722680406 70-71 22.74186322075564 27.296234178894185 27.805575042297693 22.15632755805248 72-73 22.826172259998266 27.37295488919879 27.599107621509194 22.201765229293745 74-75 22.83055677859471 27.64987960996207 27.24230360396375 22.277260007479473 76-77 22.861792655823525 27.47190961557734 27.60672430157224 22.059573427026898 78-79 22.862492704753258 27.474967724059873 27.462145775241552 22.200393795945317 80-81 22.914701617039338 27.647437673538445 27.348185178416678 22.089675531005533 82-83 22.907403286255587 27.576810218666132 27.27903107190176 22.23675542317652 84-85 22.864691814171437 27.634609425690275 27.23208084616113 22.268617913977156 86-87 23.11701575773296 27.633547228985268 27.13279117614115 22.116645837140616 88-89 22.861350519657375 27.640695097004674 27.059322883199417 22.438631500138538 90-91 22.974372693094676 27.498071936571595 27.108774466507708 22.418780903826022 92-93 23.021615073072095 27.39982432042069 27.08162615621334 22.496934450293878 94-95 22.87443038123928 27.291775972552184 27.117021652881846 22.71677199332669 96-97 23.036362752091303 27.157919248250618 27.10950533805731 22.696212661600768 98-99 22.858716657731794 27.311082740592134 26.859438816396686 22.970761785279382 100-101 23.146812689545385 26.900391711776777 27.297372988469743 22.65542261020809 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1669.0 1 1076.5 2 615.0 3 785.5 4 703.0 5 674.0 6 1219.5 7 2440.5 8 3526.0 9 3841.5 10 3257.5 11 2549.5 12 2479.0 13 2606.5 14 2691.0 15 2697.0 16 2713.5 17 2783.0 18 2927.5 19 3088.0 20 3228.5 21 3546.0 22 4032.5 23 4660.0 24 5468.5 25 6578.5 26 7962.5 27 9350.5 28 10570.0 29 12301.5 30 14603.0 31 16773.5 32 19373.0 33 21059.5 34 22076.0 35 23859.0 36 26596.5 37 29919.0 38 32917.0 39 34497.5 40 35786.0 41 38723.0 42 41109.0 43 43339.5 44 46295.0 45 48243.5 46 48387.5 47 49223.5 48 51139.0 49 51180.0 50 50391.5 51 49270.0 52 47919.5 53 47876.5 54 47922.5 55 45110.0 56 41078.5 57 37423.0 58 34273.0 59 30958.0 60 25976.5 61 21379.0 62 17521.5 63 13237.5 64 9764.0 65 7553.5 66 5755.5 67 4408.0 68 3246.0 69 2381.0 70 1796.0 71 1307.5 72 1000.5 73 811.0 74 664.0 75 532.5 76 390.0 77 272.0 78 178.0 79 120.5 80 86.5 81 57.0 82 30.5 83 16.5 84 11.0 85 8.5 86 4.5 87 3.0 88 1.5 89 2.0 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033676037988339395 2 0.0 3 0.0 4 0.0 5 0.015032629649061786 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003389710607141384 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.368936102481268E-5 22-23 2.9475744409925074E-4 24-25 5.158255271736887E-4 26-27 0.002173836150231974 28-29 0.0029844191215049136 30-31 2.5791276358684437E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010942870112184682 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.684468051240634E-5 74-75 3.684468051240634E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.4737872204962537E-4 84-85 1.1053404153721903E-4 86-87 0.0 88-89 0.0 90-91 0.008584810559390676 92-93 0.002358059552794006 94-95 0.0 96-97 0.0 98-99 4.0529148563646974E-4 100-101 0.01348515306754072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1357048.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.484222615431264 #Duplication Level Percentage of deduplicated Percentage of total 1 73.77244630190862 38.7188949459432 2 13.817835523213292 14.50436711327481 3 5.059624660111795 7.966514010354995 4 2.3433346033631017 4.919523799414094 5 1.2977310526432027 3.4055202730941896 6 0.827147258370841 2.604730850644731 7 0.5711695414511396 2.0984172555272784 8 0.40016548887853987 1.680189968105134 9 0.27923530372474914 1.3189903058499568 >10 1.5181237877014784 13.961657924817219 >50 0.0751507751729874 2.655171562333173 >100 0.034667257285305474 3.2212115822046896 >500 0.0023859827072886014 0.8397538248537114 >1k 5.614076958326121E-4 0.5087140275824326 >5k 4.2105577187445914E-4 1.5963425560004192 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8479 0.6248120921293867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7709 0.568071284140281 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5483 0.40403876649904796 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2171 0.15997960278486834 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1912 0.14089405827944185 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1635 0.12048210527556874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.684468051240634E-5 14-15 0.0 0.0 0.0 0.0 7.368936102481268E-5 16-17 0.0 0.0 0.0 0.0 7.368936102481268E-5 18-19 0.0 0.0 0.0 0.0 1.4737872204962537E-4 20-21 0.0 0.0 0.0 0.0 1.842234025620317E-4 22-23 0.0 0.0 0.0 0.0 2.9475744409925074E-4 24-25 0.0 0.0 0.0 7.368936102481268E-5 2.9475744409925074E-4 26-27 0.0 0.0 0.0 2.9475744409925074E-4 3.316021246116571E-4 28-29 0.0 0.0 0.0 8.474276517853458E-4 4.421361661488761E-4 30-31 0.0 0.0 0.0 0.002394904233306412 4.421361661488761E-4 32-33 0.0 0.0 0.0 0.004642429744563199 4.421361661488761E-4 34-35 0.0 0.0 0.0 0.007737382907605332 4.421361661488761E-4 36-37 0.0 0.0 0.0 0.012453502013193344 4.421361661488761E-4 38-39 0.0 0.0 0.0 0.019564525352087768 4.421361661488761E-4 40-41 0.0 0.0 0.0 0.028665161438652133 4.421361661488761E-4 42-43 0.0 0.0 0.0 0.03798686560829094 4.421361661488761E-4 44-45 0.0 0.0 0.0 0.04944556124764931 4.421361661488761E-4 46-47 0.0 0.0 0.0 0.06558353131208329 4.421361661488761E-4 48-49 0.0 0.0 0.0 0.08157412265446763 4.789808466612824E-4 50-51 0.0 0.0 0.0 0.10272296926858887 5.158255271736887E-4 52-53 3.684468051240634E-5 0.0 0.0 0.1232086116334868 5.158255271736887E-4 54-55 7.368936102481268E-5 0.0 0.0 0.14852090714550997 5.158255271736887E-4 56-57 7.368936102481268E-5 0.0 0.0 0.17980204090054294 5.158255271736887E-4 58-59 7.368936102481268E-5 0.0 0.0 0.21281487463965904 5.158255271736887E-4 60-61 7.368936102481268E-5 0.0 0.0 0.250985963650512 5.158255271736887E-4 62-63 7.368936102481268E-5 0.0 0.0 0.29199409306082025 5.158255271736887E-4 64-65 7.368936102481268E-5 0.0 0.0 0.3419186351551309 5.158255271736887E-4 66-67 7.368936102481268E-5 0.0 0.0 0.396154004869393 5.158255271736887E-4 68-69 7.368936102481268E-5 0.0 0.0 0.4655693829547665 5.158255271736887E-4 70-71 7.368936102481268E-5 0.0 0.0 0.5437169503215804 5.158255271736887E-4 72-73 7.368936102481268E-5 0.0 0.0 0.6247752474488744 5.158255271736887E-4 74-75 7.368936102481268E-5 0.0 0.0 0.7092969445443345 5.158255271736887E-4 76-77 7.368936102481268E-5 0.0 0.0 0.8108777287170388 5.158255271736887E-4 78-79 7.368936102481268E-5 0.0 0.0 0.9295175999669871 5.158255271736887E-4 80-81 7.368936102481268E-5 0.0 0.0 1.05526849455583 5.158255271736887E-4 82-83 7.368936102481268E-5 0.0 0.0 1.1946887656147758 5.158255271736887E-4 84-85 7.368936102481268E-5 0.0 0.0 1.3366513196290772 5.158255271736887E-4 86-87 7.368936102481268E-5 0.0 0.0 1.4931675224457792 5.158255271736887E-4 88-89 7.368936102481268E-5 0.0 0.0 1.6710536399596772 5.158255271736887E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4095 0.0 53.959305 1 GTATCAA 6675 0.0 36.44903 1 ACGGTAC 65 0.0058330265 29.242462 1 TATCAAC 8840 0.0 27.406727 2 ATCAACG 8775 0.0 27.393192 3 TCAACGC 8975 0.0 26.835688 4 CAACGCA 9185 0.0 26.222136 5 AACGCAG 9320 0.0 25.8385 6 GTAAGGT 480 0.0 25.731873 4 TAAGGTG 610 0.0 22.58434 5 GAGTACT 7450 0.0 22.347195 12-13 GTGGTAT 2105 0.0 22.122862 1 TGGTATC 1940 0.0 22.038399 2 ACGCAGA 10855 0.0 21.966717 7 CGCAGAG 10920 0.0 21.748968 8 GTACTTT 7760 0.0 21.331251 14-15 AGGTAAG 530 0.0 20.615376 2 AGTACTT 7590 0.0 20.464315 12-13 GTGTAGG 400 0.0 20.195578 1 GCAGAGT 11735 0.0 19.833721 9 >>END_MODULE