FastQCFastQC Report
Thu 26 May 2016
SRR937535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108026
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114341.0319252436314672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89120.8043132561871292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.5813040488219591No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41830.3775182170815486No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36470.3291439009553927No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24520.22129444615920563No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.18745047498885406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.18645771850119042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17180.15505051325510413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.1479207166618834No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15380.13880540709333536No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14370.12969009752478733No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13590.12265055152135419No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13280.11985278323793846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1201.8189894E-1243.5773852
TACCTGG11150.035.8141062
GTACCTG11250.035.50541
TAACGCA1605.2750693E-1132.6845174
ACCTGGG13700.027.758723
GCGGTAT1405.750662E-727.17251
CGTAAGA700.00835793827.1651212
CGTATCC700.00835978427.1638933
CGGTATA908.9046E-426.4105342
GTACATG102000.026.0602951
TACATGG103300.025.5872822
ACATGGG103350.024.745823
GTATCAA144000.024.6014941
ATAACGC2002.0370862E-823.7684043
GCGTGTC1000.001651814323.7544237
GGTATCA106600.023.731331
CATGGGG26250.023.0008454
GAGTACT79700.022.56018812-13
CATGGGA73450.022.0067794
AGTACTT82700.021.88540612-13
Produced by FastQC (version 0.11.5)