FastQCFastQC Report
Thu 26 May 2016
SRR937535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108026
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133141.2015963524321633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114011.028946974168476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.7336470443834351No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA46840.42273376256513834No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40220.36298787212574435No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.26118520684532676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25370.228965746291152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24810.22391171326304618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.1888042338356681No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20700.18681872086034082No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG15040.1357368870405568No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.13528563409161878No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12490.11272298664471772No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11920.10757870302682426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11000.032.389292
TAGGCCG753.076748E-431.6695255
GTACCGT600.003952637331.6638096
GTACCTG11800.030.201581
GAGTACT97650.023.7621612-13
TATAACG1000.001652739823.7521442
ACCTGGG14600.023.1013983
GGTATCA133150.023.0894471
GTACACG1652.422139E-623.038621
GTACATG98300.022.6226141
TACATGG99200.022.3155212
GTATCAA174750.022.1338841
GTACTTT105200.021.96451214-15
TAACGCA1302.9396213E-421.9250554
AGTACTT98700.021.92122512-13
ACATGGG100600.021.1077693
GTGTAGC5300.020.6206531
ATGGGCG1200.00476090619.7934535
ACTTTTT118500.019.75981916-17
ATGGGAT24400.019.0795925
Produced by FastQC (version 0.11.5)