##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937535_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1108026 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06890722780874 31.0 31.0 34.0 31.0 34.0 2 32.344878188778964 33.0 31.0 34.0 31.0 34.0 3 32.41459947690758 34.0 31.0 34.0 31.0 34.0 4 36.07439897619731 37.0 35.0 37.0 35.0 37.0 5 36.00570203226278 37.0 35.0 37.0 35.0 37.0 6 36.065219588710015 37.0 35.0 37.0 35.0 37.0 7 36.012482559073526 37.0 35.0 37.0 35.0 37.0 8 36.000601068927985 37.0 35.0 37.0 35.0 37.0 9 37.66379760041732 39.0 37.0 39.0 35.0 39.0 10-11 37.57811820300246 39.0 37.0 39.0 35.0 39.0 12-13 37.46276170414774 39.0 37.0 39.0 35.0 39.0 14-15 38.55723331401971 40.0 38.0 41.0 34.0 41.0 16-17 38.522774285079954 40.0 38.0 41.0 34.0 41.0 18-19 38.603831047285894 40.0 38.0 41.0 34.0 41.0 20-21 38.55800495656239 40.0 38.0 41.0 34.0 41.0 22-23 38.48969022387561 40.0 38.0 41.0 34.0 41.0 24-25 38.487338744758695 40.0 38.0 41.0 34.0 41.0 26-27 38.30283224400871 40.0 38.0 41.0 34.0 41.0 28-29 38.13378296177166 40.0 37.5 41.0 34.0 41.0 30-31 38.05662322003275 40.0 37.0 41.0 34.0 41.0 32-33 37.87360314649656 40.0 36.5 41.0 33.5 41.0 34-35 37.67644306180541 40.0 36.0 41.0 33.0 41.0 36-37 37.47168929248953 40.0 36.0 41.0 33.0 41.0 38-39 37.2985895637828 40.0 36.0 41.0 33.0 41.0 40-41 37.052320523164624 40.0 35.0 41.0 32.5 41.0 42-43 36.75185013709064 39.0 35.0 41.0 31.0 41.0 44-45 36.3161599998556 39.0 35.0 41.0 30.5 41.0 46-47 35.728427852776015 39.0 35.0 41.0 28.5 41.0 48-49 35.2095875909049 39.0 35.0 41.0 25.5 41.0 50-51 34.8785357924814 39.0 35.0 40.0 24.0 41.0 52-53 34.58889547718194 38.5 35.0 40.0 23.0 41.0 54-55 34.348359605280024 38.0 34.0 40.0 23.0 41.0 56-57 34.09352623494395 38.0 34.0 40.0 21.5 41.0 58-59 33.84036430553074 38.0 34.0 40.0 20.0 41.0 60-61 33.50778411336918 37.5 33.0 40.0 18.5 41.0 62-63 33.113271258977676 37.0 33.0 40.0 17.5 41.0 64-65 32.68423529772767 36.0 32.0 39.0 13.5 41.0 66-67 32.25674397532188 36.0 32.0 39.0 9.0 40.0 68-69 31.8245086306639 35.0 31.0 39.0 7.5 40.0 70-71 31.42814744419355 35.0 31.0 38.0 6.5 40.0 72-73 30.927235913236693 35.0 30.5 37.5 2.0 39.5 74-75 30.436683796228607 34.5 30.0 37.0 2.0 39.0 76-77 29.232172800999255 33.5 28.5 35.5 2.0 38.0 78-79 29.792261192426892 34.0 30.0 36.0 2.0 38.0 80-81 29.59689528946072 34.0 30.0 35.5 2.0 37.0 82-83 29.22425376299834 34.0 29.0 35.0 2.0 37.0 84-85 28.837418075027124 34.0 29.0 35.0 2.0 36.5 86-87 28.440768537922395 34.0 29.0 35.0 2.0 36.0 88-89 27.954685178867642 34.0 28.0 35.0 2.0 36.0 90-91 27.380853878880096 33.0 26.5 35.0 2.0 35.0 92-93 26.926070326869585 33.0 25.5 35.0 2.0 35.0 94-95 26.43553535747356 33.0 25.0 35.0 2.0 35.0 96-97 25.82306868250384 32.0 23.5 34.0 2.0 35.0 98-99 24.909421349318517 32.0 18.5 34.0 2.0 35.0 100-101 22.2259279114389 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1681336923921677 1101 2 0.05698428901032315 1101 3 0.1857790698954105 1101 4 0.02835869579014627 1101 5 -0.07468929376907596 1101 6 -0.0946350201176287 1101 7 0.019483619112804718 1101 8 -0.0716354441614726 1101 9 0.010255254234664335 1101 10-11 0.033545282306917557 1101 12-13 0.024108135769374428 1101 14-15 0.2516351043224603 1101 16-17 0.0792334393782923 1101 18-19 0.10275559927629274 1101 20-21 0.10833992986852792 1101 22-23 0.10578067836677008 1101 24-25 -0.01955327756181191 1101 26-27 -0.009506135633586155 1101 28-29 -0.018654674339131816 1101 30-31 0.043126055620767545 1101 32-33 0.18399675909913782 1101 34-35 0.10831883941126108 1101 36-37 0.13818003566718318 1101 38-39 0.09084568111510549 1101 40-41 0.08677038778468926 1101 42-43 0.24916129649333385 1101 44-45 0.17783412459690595 1101 46-47 0.11211836801503239 1101 48-49 0.36659904015575506 1101 50-51 0.46536284945936046 1101 52-53 0.42270302913146907 1101 54-55 0.4687156450654584 1101 56-57 0.4544702699124059 1101 58-59 0.3784668510437186 1101 60-61 0.5059058150454163 1101 62-63 0.31262441068745517 1101 64-65 0.3378005728494031 1101 66-67 0.21422698209040902 1101 68-69 0.37497968188614195 1101 70-71 0.3300085025916353 1101 72-73 0.1974460364126962 1101 74-75 0.2812709600496106 1101 76-77 0.2489819811930083 1101 78-79 0.2687829111066016 1101 80-81 0.2428724895151575 1101 82-83 0.3508495135927667 1101 84-85 0.3713200853932719 1101 86-87 0.19999975455440833 1101 88-89 0.022874469149996912 1101 90-91 -0.028417441682961453 1101 92-93 -0.36285275528752337 1101 94-95 -0.40656825231143756 1101 96-97 -0.36965516089612294 1101 98-99 -0.19874572330210682 1101 100-101 -0.16154211459724266 1102 1 0.3751111183669522 1102 2 0.2835092645979316 1102 3 0.24747905424502648 1102 4 0.16047438518955204 1102 5 0.10515565721876641 1102 6 0.1190610494317923 1102 7 0.16796387868874518 1102 8 0.1471657364505674 1102 9 0.23436874817178932 1102 10-11 0.2159824658550633 1102 12-13 0.3158489213304634 1102 14-15 0.3627517261320321 1102 16-17 0.3447532194793794 1102 18-19 0.24624757459718438 1102 20-21 0.24181125109154777 1102 22-23 0.25821210874570966 1102 24-25 0.19311118923402404 1102 26-27 0.060477530656157796 1102 28-29 0.14905461647806106 1102 30-31 0.22352317859532178 1102 32-33 0.3328506440313461 1102 34-35 0.36080471549107784 1102 36-37 0.23380387979818096 1102 38-39 0.37442512224210844 1102 40-41 0.4296339230520587 1102 42-43 0.44250194803424137 1102 44-45 0.4448462018436672 1102 46-47 0.3280121041142934 1102 48-49 0.5977188361276475 1102 50-51 0.5674628664323151 1102 52-53 0.7045521109388915 1102 54-55 0.6589570797868234 1102 56-57 0.6695462183587253 1102 58-59 0.5684547936354747 1102 60-61 0.5070707767019726 1102 62-63 0.5279351148885851 1102 64-65 0.45621115515388766 1102 66-67 0.6534252415913997 1102 68-69 0.7327397372576421 1102 70-71 0.6455257477714262 1102 72-73 0.5210287398731914 1102 74-75 0.3975455956271219 1102 76-77 0.44311396903420075 1102 78-79 0.5622727923620694 1102 80-81 0.5181880872165365 1102 82-83 0.652911837388185 1102 84-85 0.643443886540819 1102 86-87 0.5245236853135857 1102 88-89 0.5710710379687001 1102 90-91 0.7292881679876544 1102 92-93 0.5008599565712792 1102 94-95 0.22208782058849152 1102 96-97 0.1774866602354237 1102 98-99 0.02180124658523397 1102 100-101 0.12722289379004081 1103 1 0.3181054993711214 1103 2 0.261801923924331 1103 3 0.2207448724065273 1103 4 0.09223418163553276 1103 5 0.12705652139661794 1103 6 0.067405265055136 1103 7 0.1097235252221509 1103 8 0.17615610252344283 1103 9 0.24276589656206937 1103 10-11 0.2037543697856279 1103 12-13 0.2441736755760573 1103 14-15 0.21864281328439006 1103 16-17 0.1253878539175659 1103 18-19 0.11933567821674984 1103 20-21 -0.008869147566869628 1103 22-23 0.07235867690619102 1103 24-25 0.15891033909344543 1103 26-27 0.19640155768947665 1103 28-29 0.18197166985102342 1103 30-31 0.3324646459123315 1103 32-33 0.4018237156445821 1103 34-35 0.34104828253811803 1103 36-37 0.3704543013932238 1103 38-39 0.35493035487234437 1103 40-41 0.45826006971289246 1103 42-43 0.435820617192185 1103 44-45 0.3863319172196569 1103 46-47 0.14674054566405914 1103 48-49 0.2509979692513795 1103 50-51 0.19956650780873275 1103 52-53 0.29038557714928004 1103 54-55 -0.08299057074243166 1103 56-57 0.13569221140004828 1103 58-59 0.0844821513173315 1103 60-61 0.12935071526506903 1103 62-63 -0.028536610918585836 1103 64-65 0.1035854364253126 1103 66-67 0.11554710415973801 1103 68-69 0.149663640272383 1103 70-71 0.1187455700296276 1103 72-73 0.1626409269477307 1103 74-75 0.2549330828334462 1103 76-77 0.2883507014307156 1103 78-79 0.28571952774320764 1103 80-81 0.2656388920426309 1103 82-83 0.12056274824335489 1103 84-85 0.054472136883912725 1103 86-87 0.07728371207979379 1103 88-89 0.16642429332023667 1103 90-91 0.17614552344808132 1103 92-93 0.20653830415311702 1103 94-95 0.1809061551688238 1103 96-97 -0.1528163948465462 1103 98-99 -0.18268359248361676 1103 100-101 -0.2704191858516971 1104 1 0.19983470002365067 1104 2 0.07746260907930491 1104 3 0.10655237648482796 1104 4 0.15014609028153103 1104 5 0.07566989823185111 1104 6 -0.006004196608806467 1104 7 0.11624881070338944 1104 8 0.06441058865721061 1104 9 0.01927486882960494 1104 10-11 0.09364017728971419 1104 12-13 0.17361902631015624 1104 14-15 0.10281899599238642 1104 16-17 0.16902570057334998 1104 18-19 0.07732915293127718 1104 20-21 -0.09369785882297776 1104 22-23 0.01934073237111278 1104 24-25 0.06266237824515741 1104 26-27 0.013285733872187677 1104 28-29 -0.03050793863184964 1104 30-31 -0.09331023173856323 1104 32-33 -0.07860042791169519 1104 34-35 -0.0859500861405138 1104 36-37 0.0070774661566801456 1104 38-39 -0.08715773648165026 1104 40-41 -0.2228166023915108 1104 42-43 -0.18408150352559716 1104 44-45 -0.21481000773955827 1104 46-47 -0.17055146086121908 1104 48-49 -0.2983495022004945 1104 50-51 -0.09978096364314837 1104 52-53 -0.1810662988703058 1104 54-55 -0.20370835214536953 1104 56-57 -0.2060696156798869 1104 58-59 -0.1655178486826614 1104 60-61 -0.04193802861747287 1104 62-63 -0.033022744686938665 1104 64-65 -0.09380444938219057 1104 66-67 -0.1360842172105805 1104 68-69 -0.04609492416481231 1104 70-71 -0.21200483780071622 1104 72-73 -0.10000181367216854 1104 74-75 -0.19204897263147913 1104 76-77 -0.010996770021158397 1104 78-79 -0.16411734011976264 1104 80-81 -0.12057644237825116 1104 82-83 -0.18612566937491692 1104 84-85 -0.12415755316502342 1104 86-87 -0.2542823564357022 1104 88-89 -0.22631632658188394 1104 90-91 -0.08405023511635434 1104 92-93 0.0540097560291386 1104 94-95 0.058557052395574516 1104 96-97 0.11798295262490299 1104 98-99 0.3205790502570025 1104 100-101 0.134556344327752 1105 1 0.3122026216349312 1105 2 0.16145166963011803 1105 3 0.13352382302973353 1105 4 0.046746437870268664 1105 5 0.023511596085540987 1105 6 0.06108522752408163 1105 7 0.055194209417521733 1105 8 0.0865853639506895 1105 9 0.04243056939150591 1105 10-11 0.09024225749101333 1105 12-13 0.1681113175170097 1105 14-15 0.12470255909680361 1105 16-17 0.21303605893094613 1105 18-19 0.14499567032670058 1105 20-21 -0.011668649640668605 1105 22-23 0.03894457717167654 1105 24-25 -0.024691282631913225 1105 26-27 -0.21047251602918493 1105 28-29 -0.21215170815705875 1105 30-31 -0.14201996848807852 1105 32-33 -0.024227713627503533 1105 34-35 -0.1463362988093735 1105 36-37 -0.1208726399325144 1105 38-39 -0.23597649454496405 1105 40-41 -0.08592888101693319 1105 42-43 -0.10276133271665344 1105 44-45 -0.18285663367650073 1105 46-47 -0.21094865562820075 1105 48-49 -0.13800220667543073 1105 50-51 0.05421897238347384 1105 52-53 -0.02360713521571256 1105 54-55 -0.07894158845240185 1105 56-57 0.032653742069960856 1105 58-59 0.09086682862490392 1105 60-61 -0.07183359206307927 1105 62-63 -0.1305708313495515 1105 64-65 -0.2873266108296946 1105 66-67 -0.19680695655100777 1105 68-69 -0.20706995855050891 1105 70-71 -0.12264467829373515 1105 72-73 0.01811704888100607 1105 74-75 -0.009099079786903275 1105 76-77 -0.0793103515712339 1105 78-79 -0.07160049379449873 1105 80-81 -0.07119898340118525 1105 82-83 0.13540790599106245 1105 84-85 0.15599057188162035 1105 86-87 0.049905767971210935 1105 88-89 0.05792542884785945 1105 90-91 0.201534961441876 1105 92-93 0.31780734956356227 1105 94-95 0.21494066348003216 1105 96-97 0.30211502035139404 1105 98-99 0.3646467181071742 1105 100-101 0.3495649007682715 1106 1 0.09351654669604414 1106 2 0.024758060888913747 1106 3 0.015633537144388754 1106 4 -0.05060675276985194 1106 5 -0.028046304428350766 1106 6 -0.06557994236066378 1106 7 -0.09212655094768962 1106 8 -0.07440600460691371 1106 9 -0.11766273001477145 1106 10-11 -0.1608023715565352 1106 12-13 -0.2521008513199874 1106 14-15 -0.06282238726851119 1106 16-17 0.05890182253919107 1106 18-19 0.005327207521737876 1106 20-21 0.130422728895482 1106 22-23 0.1197520910169132 1106 24-25 0.019286041122015263 1106 26-27 -0.024824783653798477 1106 28-29 0.08649682250202062 1106 30-31 0.13498759026061435 1106 32-33 0.00153252685545624 1106 34-35 -0.3021918344078429 1106 36-37 0.08205045042321757 1106 38-39 0.1474709684096851 1106 40-41 -0.0918255781181685 1106 42-43 -0.2346808307957886 1106 44-45 -0.0811315925149998 1106 46-47 0.14053004017581827 1106 48-49 0.15922438116071902 1106 50-51 0.20484512615286832 1106 52-53 0.21346752925566648 1106 54-55 0.18733591491535861 1106 56-57 0.2705110987530901 1106 58-59 0.11344640753025459 1106 60-61 0.08641991290124906 1106 62-63 0.012250228372046479 1106 64-65 -0.3168506341794952 1106 66-67 -0.1673640088884838 1106 68-69 0.010702455758728746 1106 70-71 0.2323171537673332 1106 72-73 0.27325600013580953 1106 74-75 0.2020313647573282 1106 76-77 0.16120691131356324 1106 78-79 0.3372860251896874 1106 80-81 0.04909550844135424 1106 82-83 0.06988939578799958 1106 84-85 -0.07502212581832524 1106 86-87 0.3103208754900457 1106 88-89 0.3094596303147057 1106 90-91 0.008886655300081259 1106 92-93 0.16501398030746373 1106 94-95 0.3278260848241423 1106 96-97 0.26015835218795047 1106 98-99 -0.102396015016609 1106 100-101 -0.022987237135236427 1107 1 0.07111611212475566 1107 2 0.04533893240039788 1107 3 0.06310895296185492 1107 4 -0.0291382231102304 1107 5 -0.10136251086060355 1107 6 -0.07837542565104627 1107 7 -0.06568672722679736 1107 8 -0.04186489255641135 1107 9 -0.032431210526453924 1107 10-11 -0.05757126866851081 1107 12-13 -0.032709741243571955 1107 14-15 0.020672276571808368 1107 16-17 -0.14452709741773617 1107 18-19 0.002900442722548746 1107 20-21 -0.006248035702213883 1107 22-23 -0.05606140274838367 1107 24-25 -0.16911225326735746 1107 26-27 0.06489165585679757 1107 28-29 0.14310340887102768 1107 30-31 -0.13367365243356488 1107 32-33 -0.22548808669058928 1107 34-35 0.007651232532744245 1107 36-37 0.0797976094406323 1107 38-39 -0.0762896797441357 1107 40-41 0.10302151187430297 1107 42-43 0.18160156974770558 1107 44-45 0.16030291478708136 1107 46-47 0.21304538018837604 1107 48-49 0.2211923138293983 1107 50-51 0.27545240957856976 1107 52-53 0.3271197660316716 1107 54-55 0.045773840790459985 1107 56-57 0.02428841091877132 1107 58-59 -0.03642350698499541 1107 60-61 0.08560747425108417 1107 62-63 -0.013770272174575382 1107 64-65 0.22458339661897497 1107 66-67 0.20400111948838884 1107 68-69 0.07817026113287184 1107 70-71 0.1140237718578625 1107 72-73 0.06671176766739606 1107 74-75 -0.02937376269377623 1107 76-77 -0.10356640230613579 1107 78-79 -0.00794253997626626 1107 80-81 0.06250534102521499 1107 82-83 0.042148036500197605 1107 84-85 0.17478863417770185 1107 86-87 -0.19349796056140178 1107 88-89 -0.15297690961561727 1107 90-91 -0.05962231045564792 1107 92-93 -0.23722824145187005 1107 94-95 -0.17469768249506146 1107 96-97 -0.03151665329184539 1107 98-99 -0.044499805670877635 1107 100-101 -0.1035694428568199 1108 1 0.16682302571403795 1108 2 0.0833236757615623 1108 3 0.039766504684372705 1108 4 -0.019592366924179316 1108 5 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64-65 -1.2430901283579203 2316 66-67 -1.04598996308696 2316 68-69 -0.9364445871898432 2316 70-71 -1.018604274253331 2316 72-73 -1.1139982214695578 2316 74-75 -1.0578884440174434 2316 76-77 -0.9773272856175801 2316 78-79 -1.0783666851195157 2316 80-81 -0.8663490131100389 2316 82-83 -1.0717445004260497 2316 84-85 -0.9480424049454719 2316 86-87 -0.9850912611434666 2316 88-89 -0.7635837808966457 2316 90-91 -0.7647157812794525 2316 92-93 -0.4719356688559273 2316 94-95 -0.6668997999780757 2316 96-97 -0.3900344151803914 2316 98-99 -0.23721751243352784 2316 100-101 0.03517838858764222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 12.0 5 36.0 6 77.0 7 174.0 8 384.0 9 734.0 10 1255.0 11 2166.0 12 3514.0 13 5389.0 14 6677.0 15 9380.0 16 13828.0 17 19998.0 18 17895.0 19 9311.0 20 6850.0 21 6642.0 22 6749.0 23 7318.0 24 8191.0 25 9732.0 26 11533.0 27 14063.0 28 17300.0 29 21495.0 30 26545.0 31 32954.0 32 42095.0 33 55268.0 34 76079.0 35 108446.0 36 156738.0 37 227197.0 38 168411.0 39 13579.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12151199684429 19.833223960651107 15.394197500886866 19.651066541617734 2 15.360740632440034 22.090997864671046 40.50789421908872 22.0403672838002 3 19.72805692285199 28.060532875582346 30.535023546378877 21.676386655186793 4 12.191681422638096 17.904724257373022 37.666444650215794 32.23714966977309 5 12.237212632392467 39.74721852803863 33.06852582663189 14.947043012937018 6 28.570177956112943 37.514372406423675 18.157064906419162 15.758384731044217 7 26.62951952391009 35.838689705837226 20.332465122659578 17.19932564759311 8 26.694138946198017 34.21074956724842 19.605135619561274 19.48997586699229 9 27.829310864546496 16.780833662747984 20.876495677899253 34.513359794806256 10-11 24.667652203107146 28.118925007174923 26.101463323062816 21.111959466655115 12-13 26.897224274253922 26.01810230414705 27.485862792711607 19.598810628887424 14-15 23.666096282939208 25.87209144911762 28.321988834197033 22.139823433746137 16-17 20.092127802055188 29.74894090932884 28.848736401492385 21.310194887123586 18-19 19.974034905318106 27.12549164008787 33.44343905287421 19.457034401719817 20-21 21.472781324625956 25.750884907032866 33.48251755825225 19.29381621008893 22-23 20.86788262109342 25.910758710256193 33.11357165967897 20.10778700897142 24-25 20.55021445864141 25.97880458203692 33.816145430259766 19.65483552906191 26-27 20.6587284360705 26.031767663480636 33.756538164923086 19.552965735525785 28-29 19.985072397518262 26.008684904394304 34.32459593263445 19.681646765452978 30-31 19.978412058922803 26.498926920487424 34.037648936035794 19.485012084553972 32-33 19.81659275143363 26.56530622927621 33.50990861225278 20.108192407037382 34-35 20.41188564167267 26.15953957759114 33.5600428148798 19.8685319658564 36-37 20.78290581628951 26.102636580730053 33.41577724710431 19.698680355876125 38-39 20.785929211047392 26.134856041284234 33.58359821881436 19.495616528854015 40-41 20.37920829877466 26.31981040036317 33.67929611315329 19.621685187708877 42-43 20.121266825377127 26.823662149812144 33.06996423373312 19.985106791077616 44-45 20.286121444803644 27.144669890417735 32.23692404329862 20.33228462148 46-47 20.504663247974324 27.239026882040672 31.349715620391578 20.90659424959342 48-49 20.907632131375976 27.38112643566126 31.05752933595421 20.653712097008555 50-51 21.362991482149337 27.771369985902854 30.538588444675486 20.32705008727232 52-53 21.12784357045773 28.175467001676857 30.349739085544925 20.34695034232049 54-55 20.464520204869025 28.22084339252273 30.488301256740595 20.826335145867645 56-57 21.04011999718418 28.605691563194362 29.749347036982886 20.604841402638566 58-59 21.05695173217957 27.872721398234333 30.086162238070223 20.984164631515867 60-61 21.173961621839197 27.862523081588336 30.422526186208625 20.540989110363835 62-63 21.71077212989587 27.521962481024815 30.413591377819653 20.353674011259663 64-65 21.244853460117362 27.55228667919345 30.619859100779223 20.583000759909968 66-67 20.891387025214208 28.08259914478541 30.326093431020574 20.699920398979806 68-69 20.925591998743712 28.358495197766114 30.07943856913105 20.636474234359124 70-71 20.913543545007066 28.400732473786718 29.67520617747237 21.010517803733848 72-73 21.12824969811178 28.224157194867267 29.428957443236893 21.218635663784063 74-75 21.04011999718418 28.518148491100387 29.443893915846743 20.997837595868692 76-77 21.189123720923515 27.942936357089092 30.0499266262705 20.818013295716888 78-79 21.020806370969634 28.132868723297104 29.903179167275855 20.9431457384574 80-81 20.77009023253967 28.898960854709188 29.58238344587582 20.748565466875327 82-83 20.915166877027552 28.823352359824966 29.10547812239258 21.1560026407549 84-85 20.8091687379177 28.28011256053558 29.227698628010536 21.68302007353618 86-87 21.102483154727416 28.16747982448065 29.470788591603448 21.259248429188485 88-89 21.444623140612222 27.74470093662062 29.61830318061128 21.19237274215587 90-91 20.835121667661817 28.170699840099218 29.818269127053647 21.175909365185316 92-93 20.760619997761744 28.485907993111937 29.563521431330575 21.18995057779575 94-95 20.494103928969174 28.50366327143948 29.228420632728835 21.77381216686251 96-97 20.817155915113904 28.134222482143922 28.945710660219166 22.10291094252301 98-99 20.764516140677685 28.081611324105964 28.901912260561296 22.251960274655055 100-101 21.017780995331336 28.219860409527968 29.324425082956314 21.43793351218438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1860.0 1 1293.5 2 1560.5 3 2033.5 4 1307.0 5 992.5 6 1524.0 7 2980.5 8 4464.0 9 5007.0 10 4340.5 11 3446.0 12 3316.5 13 3511.0 14 3719.5 15 3790.5 16 3838.0 17 3977.5 18 4030.5 19 4149.5 20 4389.5 21 4751.0 22 5361.0 23 6034.0 24 6820.0 25 7722.0 26 9188.5 27 10884.0 28 11852.0 29 13257.5 30 15071.0 31 16548.5 32 18395.5 33 21186.0 34 24364.5 35 26418.5 36 29113.5 37 32112.5 38 33638.0 39 34194.0 40 34062.5 41 34766.0 42 35421.0 43 36612.5 44 38328.0 45 38547.5 46 37599.5 47 38941.5 48 51165.0 49 51110.5 50 39713.5 51 35363.0 52 30538.5 53 28113.5 54 26501.0 55 24133.5 56 21424.0 57 19233.5 58 17765.5 59 16117.0 60 13870.0 61 11487.0 62 9097.0 63 6599.5 64 4724.0 65 3698.0 66 2683.0 67 1997.0 68 1572.5 69 1169.0 70 879.0 71 669.0 72 547.0 73 523.0 74 433.5 75 330.0 76 253.0 77 172.0 78 111.5 79 71.5 80 50.5 81 39.5 82 32.5 83 16.5 84 10.0 85 9.5 86 4.5 87 1.0 88 1.0 89 1.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017418363829007626 2 0.0 3 0.0 4 0.0 5 0.03871750301888223 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004106401835335994 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.768794234070319E-4 24-25 3.1587706425661495E-4 26-27 0.0011281323723450532 28-29 0.001037881782557449 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.317541285132299E-4 42-43 0.012499706685583192 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 9.025058978760426E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.573806029822405E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.013672964352822047 92-93 0.001985512975327294 94-95 0.0 96-97 0.0 98-99 0.0016245106161768765 100-101 0.01931362621454731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1108026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.02376709124154 #Duplication Level Percentage of deduplicated Percentage of total 1 77.32668394837947 40.22825395668539 2 12.996791583908188 13.522841165892954 3 4.12323906298044 6.435192860220101 4 1.7425762309841781 3.6262151991781755 5 0.9445270746254614 2.456892827084336 6 0.5756668769742237 1.7969015717909647 7 0.3885030711334158 1.4147975300813804 8 0.26834802191600693 1.1168379993242978 9 0.22152616195961328 1.0372162908963214 >10 1.2363538957520313 12.174221960968222 >50 0.11460048788041273 4.130574601135549 >100 0.055463872539112495 5.390000787172324 >500 0.002599868621604375 0.9521949158527393 >1k 0.002599868621604375 2.7563157374797855 >5k 1.7332457477362503E-4 0.7329917112658066 >10k+ 3.4664914954725005E-4 2.2285508849716336 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13314 1.2015963524321633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11401 1.028946974168476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8129 0.7336470443834351 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4684 0.42273376256513834 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4022 0.36298787212574435 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2894 0.26118520684532676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2537 0.228965746291152 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2481 0.22391171326304618 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2092 0.1888042338356681 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2070 0.18681872086034082 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1504 0.1357368870405568 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 0.13528563409161878 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1249 0.11272298664471772 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1192 0.10757870302682426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.2562647446901067E-4 0.0 24-25 0.0 0.0 0.0 4.5125294893802134E-4 0.0 26-27 0.0 0.0 0.0 6.317541285132298E-4 0.0 28-29 0.0 0.0 0.0 0.0010830070774512511 0.0 30-31 0.0 0.0 0.0 0.002075763565114898 0.0 32-33 0.0 0.0 0.0 0.003971025950654588 0.0 34-35 0.0 0.0 0.0 0.004918657143424432 0.0 36-37 0.0 0.0 0.0 0.00762617483705256 0.0 38-39 0.0 0.0 0.0 0.012635082570264597 0.0 40-41 0.0 0.0 0.0 0.01714761205964481 0.0 42-43 0.0 0.0 0.0 0.021073512715405595 0.0 44-45 0.0 0.0 0.0 0.023645654524352317 0.0 46-47 0.0 0.0 0.0 0.028113058718838728 0.0 48-49 0.0 0.0 0.0 0.034972103542696656 0.0 50-51 0.0 0.0 0.0 0.042192150725704994 0.0 52-53 0.0 0.0 0.0 0.05794087864364193 0.0 54-55 0.0 0.0 0.0 0.06985395649560569 0.0 56-57 0.0 0.0 0.0 0.09372523749442702 0.0 58-59 0.0 0.0 0.0 0.13442825348863655 0.0 60-61 0.0 0.0 0.0 0.17829004012541222 0.0 62-63 0.0 0.0 0.0 0.2073958553319146 0.0 64-65 0.0 0.0 0.0 0.25252115022571675 0.0 66-67 0.0 0.0 0.0 0.321337224938765 0.0 68-69 0.0 0.0 0.0 0.41212931826509486 0.0 70-71 0.0 0.0 0.0 0.5113598417365658 0.0 72-73 0.0 0.0 0.0 0.6197959253663723 0.0 74-75 0.0 0.0 0.0 0.7403255880277178 0.0 76-77 0.0 0.0 0.0 0.8485360451830553 0.0 78-79 0.0 0.0 0.0 0.9789030221312496 0.0 80-81 0.0 0.0 0.0 1.1485290056370518 0.0 82-83 0.0 0.0 0.0 1.3080469230866423 9.025058978760426E-5 84-85 0.0 0.0 0.0 1.492383752727824 9.025058978760426E-5 86-87 0.0 0.0 0.0 1.7214397496087637 9.025058978760426E-5 88-89 0.0 0.0 0.0 1.9868216088792139 9.025058978760426E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1100 0.0 32.38929 2 TAGGCCG 75 3.076748E-4 31.669525 5 GTACCGT 60 0.0039526373 31.663809 6 GTACCTG 1180 0.0 30.20158 1 GAGTACT 9765 0.0 23.76216 12-13 TATAACG 100 0.0016527398 23.752144 2 ACCTGGG 1460 0.0 23.101398 3 GGTATCA 13315 0.0 23.089447 1 GTACACG 165 2.422139E-6 23.03862 1 GTACATG 9830 0.0 22.622614 1 TACATGG 9920 0.0 22.315521 2 GTATCAA 17475 0.0 22.133884 1 GTACTTT 10520 0.0 21.964512 14-15 TAACGCA 130 2.9396213E-4 21.925055 4 AGTACTT 9870 0.0 21.921225 12-13 ACATGGG 10060 0.0 21.107769 3 GTGTAGC 530 0.0 20.620653 1 ATGGGCG 120 0.004760906 19.793453 5 ACTTTTT 11850 0.0 19.759819 16-17 ATGGGAT 2440 0.0 19.079592 5 >>END_MODULE