FastQCFastQC Report
Thu 26 May 2016
SRR937534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077788
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112011.0392581843553648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87240.8094356218477103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.5780357547124295No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42870.39775911403726893No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35960.33364632005552114No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23770.22054429999220623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.18547246768381168No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.18435907618195785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16040.14882333074779083No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.14436976474037566No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14990.13908115510657013No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13830.1283183705886501No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13210.12256584782907214No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12890.11959680382412868No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.10428767067363898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA1056.8728434E-527.1617224
GTACCTG10650.025.8913421
TACCTGG10700.025.3225042
GTACATG99500.025.037121
CATGGGG26500.024.753044
TACATGG100550.024.6303842
TAGCCCT5200.023.7665064
ACATGGG101400.023.4808773
GAGTACT78650.023.10069512-13
GTATCAA148650.022.9953581
ATAACGC1252.2480154E-422.8116023
GGTATCA112250.022.659151
GTGTAGC4750.022.0193881
AGTACTT79450.021.91151412-13
GTACTTT84050.021.53176514-15
ACCTGGG12900.020.999053
ATAGCCC3252.1827873E-1120.471953
CATGGGA72450.020.4040954
GGACCGA1456.1361905E-419.6596856
ATGGGAG22300.019.6100315
Produced by FastQC (version 0.11.5)