##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937534_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1077788 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63321450971805 33.0 31.0 34.0 30.0 34.0 2 31.71632454620018 33.0 31.0 34.0 30.0 34.0 3 31.7418620359477 33.0 31.0 34.0 30.0 34.0 4 35.357037747683215 37.0 35.0 37.0 33.0 37.0 5 35.35582879007745 37.0 35.0 37.0 33.0 37.0 6 35.45702401585469 37.0 35.0 37.0 33.0 37.0 7 35.39953033435147 37.0 35.0 37.0 33.0 37.0 8 35.39409791164867 37.0 35.0 37.0 33.0 37.0 9 36.966623306253176 39.0 37.0 39.0 33.0 39.0 10-11 36.858689278410964 39.0 37.0 39.0 33.0 39.0 12-13 36.71843767048807 39.0 37.0 39.0 32.5 39.0 14-15 37.75188951816127 40.0 37.5 41.0 32.5 41.0 16-17 37.70730189981703 40.0 37.5 41.0 32.5 41.0 18-19 37.75358094541784 40.0 37.5 41.0 33.0 41.0 20-21 37.627061629930935 40.0 37.0 41.0 32.0 41.0 22-23 37.48033379477226 40.0 37.0 41.0 32.0 41.0 24-25 37.32192741058539 40.0 37.0 41.0 32.0 41.0 26-27 37.37812445490208 40.0 37.0 41.0 32.0 41.0 28-29 37.30541442287351 40.0 37.0 41.0 32.0 41.0 30-31 37.03594723637673 39.0 36.0 41.0 31.0 41.0 32-33 36.73124399232502 39.0 35.0 41.0 31.0 41.0 34-35 36.42818624813043 39.0 35.0 40.5 30.0 41.0 36-37 36.353821901895365 39.0 35.0 41.0 30.0 41.0 38-39 36.26450841909541 39.0 35.0 41.0 30.0 41.0 40-41 36.0294018860852 39.0 35.0 41.0 30.0 41.0 42-43 35.7220561928691 39.0 35.0 40.5 28.5 41.0 44-45 35.36182301157556 39.0 35.0 40.0 26.5 41.0 46-47 34.82133592135003 38.0 35.0 40.0 24.0 41.0 48-49 34.35286067389876 38.0 34.0 40.0 22.0 41.0 50-51 33.401660623425016 37.5 33.0 39.5 18.5 40.5 52-53 33.482348105564355 38.0 33.5 39.5 18.0 40.5 54-55 33.77353709634919 38.0 34.0 40.0 17.5 41.0 56-57 33.51856858677217 38.0 33.5 40.0 13.0 41.0 58-59 33.292022178758714 38.0 33.0 40.0 11.0 41.0 60-61 32.978066187413475 37.5 33.0 40.0 8.0 41.0 62-63 32.60204650636303 37.0 32.0 40.0 7.0 41.0 64-65 32.223794011438244 36.0 32.0 39.5 3.5 41.0 66-67 31.813364966023002 36.0 31.0 39.0 2.0 41.0 68-69 31.341170063129297 35.0 31.0 39.0 2.0 40.0 70-71 30.897174583498796 35.0 30.5 38.0 2.0 40.0 72-73 30.431233229540503 35.0 30.0 37.5 2.0 40.0 74-75 29.938192390340216 35.0 29.0 37.0 2.0 39.0 76-77 29.45957182674144 34.0 29.0 36.5 2.0 39.0 78-79 28.92628420431476 34.0 28.5 36.0 2.0 38.5 80-81 28.398436891114024 34.0 27.5 35.5 2.0 37.0 82-83 27.879652584738373 34.0 26.0 35.0 2.0 37.0 84-85 27.405140435781433 33.5 26.0 35.0 2.0 36.0 86-87 26.861700538510355 33.0 25.0 35.0 2.0 36.0 88-89 26.340302081670977 33.0 24.5 35.0 2.0 35.5 90-91 25.806842347474642 32.0 22.0 35.0 2.0 35.0 92-93 25.25634076460306 32.0 19.5 35.0 2.0 35.0 94-95 24.619107839389564 32.0 14.5 34.0 2.0 35.0 96-97 23.83572279520648 31.0 2.0 34.0 2.0 35.0 98-99 22.915962137266327 31.0 2.0 34.0 2.0 35.0 100-101 20.50039386224378 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27898521718552516 1101 2 0.379920188480515 1101 3 0.5068493791073294 1101 4 0.37663591300150046 1101 5 0.26701101953517536 1101 6 0.16470882265951303 1101 7 0.3444140775623836 1101 8 0.39463661382450965 1101 9 0.5538547523280926 1101 10-11 0.4930679525953465 1101 12-13 0.6200513475772524 1101 14-15 0.7163124267628973 1101 16-17 0.7287811020058257 1101 18-19 0.6721322423405738 1101 20-21 0.668313223816078 1101 22-23 0.7191565029816545 1101 24-25 0.5353167165254362 1101 26-27 0.5600733258602162 1101 28-29 0.5488288216469996 1101 30-31 0.6180185458555272 1101 32-33 0.7044783739217024 1101 34-35 0.8715585808441801 1101 36-37 0.7965436023179606 1101 38-39 0.8027366923622665 1101 40-41 0.7202752097606293 1101 42-43 0.6656767785011581 1101 44-45 0.8029130744002089 1101 46-47 0.9203871047775607 1101 48-49 1.3441694267213435 1101 50-51 1.307031872031395 1101 52-53 1.110040292955624 1101 54-55 1.303433141956134 1101 56-57 1.4423199320747315 1101 58-59 1.3453275229772999 1101 60-61 1.2690837729660487 1101 62-63 1.3482154467319987 1101 64-65 1.1351336098299427 1101 66-67 1.2155947671879552 1101 68-69 1.3142867977355301 1101 70-71 1.3186553558607166 1101 72-73 1.2247149566296613 1101 74-75 1.3313116022915317 1101 76-77 1.370141596693145 1101 78-79 1.486614689165588 1101 80-81 1.6739015771381212 1101 82-83 1.6065025990426243 1101 84-85 1.5945980734028602 1101 86-87 1.8297838161168336 1101 88-89 1.7957921624808968 1101 90-91 1.9837090287243306 1101 92-93 1.9384867058517408 1101 94-95 1.9581284540329413 1101 96-97 2.0376662484984145 1101 98-99 2.5406391214207105 1101 100-101 2.809175312431776 1102 1 0.16574206203331698 1102 2 0.21100116109833422 1102 3 0.25849947790038286 1102 4 0.1115466781992609 1102 5 0.13186937660420028 1102 6 0.3045601480079725 1102 7 0.10030833444387355 1102 8 0.2035845916552219 1102 9 0.3652350424328006 1102 10-11 0.21351047290357883 1102 12-13 0.39242460735727747 1102 14-15 0.3723087919042669 1102 16-17 0.4704552307878558 1102 18-19 0.411635436451661 1102 20-21 0.43033467422796434 1102 22-23 0.4049869055589994 1102 24-25 0.36237268343761286 1102 26-27 0.45786386503790055 1102 28-29 0.3769411170029926 1102 30-31 0.50514564656023 1102 32-33 0.4137022464559763 1102 34-35 0.2319679351612436 1102 36-37 0.12626080501227221 1102 38-39 0.34144849511395137 1102 40-41 0.3995142903535722 1102 42-43 0.6184745657600317 1102 44-45 0.33240128379263467 1102 46-47 0.4337585408468314 1102 48-49 0.36936287755478503 1102 50-51 0.4334321964327543 1102 52-53 0.3316522075866999 1102 54-55 0.454503115078559 1102 56-57 0.5607920351628977 1102 58-59 0.559213618177381 1102 60-61 0.6718404866015888 1102 62-63 0.7399398619126956 1102 64-65 0.8228547251620242 1102 66-67 0.6181403547222324 1102 68-69 0.558390796010233 1102 70-71 0.6650249989284873 1102 72-73 0.6871983718892274 1102 74-75 0.6408130445794278 1102 76-77 0.92013730437737 1102 78-79 0.8745060314599762 1102 80-81 0.8374890597682061 1102 82-83 0.7655838480437005 1102 84-85 0.9666605416030833 1102 86-87 1.3209482760213689 1102 88-89 1.3364567944270327 1102 90-91 1.3522130624542577 1102 92-93 1.7201931351824271 1102 94-95 1.9190812923005787 1102 96-97 1.8962620121572265 1102 98-99 2.231084286912168 1102 100-101 2.3945585464369685 1103 1 0.18880139810055852 1103 2 0.14134045679960394 1103 3 0.15010343737579035 1103 4 0.10912841177562882 1103 5 -0.0037272043212368544 1103 6 0.13115807036363947 1103 7 0.13310226970243377 1103 8 0.24375229094512463 1103 9 0.15464827268157677 1103 10-11 0.20042947704547487 1103 12-13 0.20232780832977681 1103 14-15 0.24954126135288845 1103 16-17 0.10450185129985101 1103 18-19 3.6605510943843456E-4 1103 20-21 0.08345496947936226 1103 22-23 0.21203050819482172 1103 24-25 0.33028569968655574 1103 26-27 0.0755094156874847 1103 28-29 0.26977608826685184 1103 30-31 0.18275490612082734 1103 32-33 0.27772449970323265 1103 34-35 0.2572428189654872 1103 36-37 0.10636053029939774 1103 38-39 0.3593660238193266 1103 40-41 0.2103541678882337 1103 42-43 0.2766888089869042 1103 44-45 0.12581419133239535 1103 46-47 0.3334883404253972 1103 48-49 0.37206556547466363 1103 50-51 0.231135697962209 1103 52-53 0.27178831968158335 1103 54-55 0.3175377557024248 1103 56-57 0.585526449753722 1103 58-59 0.4965660232482776 1103 60-61 0.6009333871402092 1103 62-63 0.770811217249495 1103 64-65 0.7145297900114898 1103 66-67 0.6510125742628112 1103 68-69 0.6574860484958442 1103 70-71 0.7462170805246409 1103 72-73 0.7069164390810698 1103 74-75 0.7073943392168509 1103 76-77 0.8030920471741894 1103 78-79 0.6549610101351995 1103 80-81 0.8366262234021811 1103 82-83 0.5993555505661625 1103 84-85 0.9020753252308005 1103 86-87 0.7053975990845345 1103 88-89 0.7716462970859617 1103 90-91 0.9310343951604025 1103 92-93 1.1894151056490827 1103 94-95 1.1210649954958107 1103 96-97 1.2764897954882137 1103 98-99 1.2866090377356905 1103 100-101 1.5777259900210083 1104 1 0.27434415118570854 1104 2 0.2651528711010336 1104 3 0.3443539779881064 1104 4 0.2892972887653258 1104 5 0.341935431915779 1104 6 0.2844629865484052 1104 7 0.29693218954547973 1104 8 0.4733092869291511 1104 9 0.3283636748483616 1104 10-11 0.4407980418620383 1104 12-13 0.40874280347917136 1104 14-15 0.5715023155875514 1104 16-17 0.3992595377501189 1104 18-19 0.48092772285100693 1104 20-21 0.6385920058941323 1104 22-23 0.5655448947150106 1104 24-25 0.5232700693515682 1104 26-27 0.6533880746437717 1104 28-29 0.48494656405988934 1104 30-31 0.732374038648075 1104 32-33 0.8779301762680305 1104 34-35 0.9058292329308983 1104 36-37 0.9914607671390812 1104 38-39 0.9380616983788457 1104 40-41 0.9208569508899771 1104 42-43 0.8396735309306109 1104 44-45 1.0207247125422896 1104 46-47 0.7372105713793147 1104 48-49 0.7782259715165125 1104 50-51 0.552060239864403 1104 52-53 0.5717150186133679 1104 54-55 0.6659041309713771 1104 56-57 0.8137888215107338 1104 58-59 0.6993559350702157 1104 60-61 0.8172081211697915 1104 62-63 0.641614358491573 1104 64-65 0.8158485317803681 1104 66-67 0.8876036274677297 1104 68-69 0.9116551314330898 1104 70-71 0.8157839945090224 1104 72-73 0.7099487457478091 1104 74-75 0.7182296191158315 1104 76-77 0.7639918211738532 1104 78-79 0.8071853791498462 1104 80-81 1.0201972996949102 1104 82-83 1.0178551955776243 1104 84-85 0.992219380121238 1104 86-87 0.8365448624209932 1104 88-89 1.2557653022509108 1104 90-91 1.2834519754934952 1104 92-93 1.3036001495213299 1104 94-95 1.278752394215683 1104 96-97 1.3857856174628118 1104 98-99 1.8275885351152787 1104 100-101 1.6517742955604504 1105 1 0.10741427897120559 1105 2 0.14741468259217427 1105 3 0.09482215172833364 1105 4 0.17984290908870548 1105 5 0.1424818166044446 1105 6 0.11566587912505355 1105 7 0.12538063720146653 1105 8 0.07487500732978702 1105 9 0.039513444037339696 1105 10-11 0.09949699659250655 1105 12-13 0.23929325568313686 1105 14-15 -0.017218655593147503 1105 16-17 0.0992540097511494 1105 18-19 0.04480716333561219 1105 20-21 0.2725176768294162 1105 22-23 0.16090560688507338 1105 24-25 0.2182620435160203 1105 26-27 0.2678562128956088 1105 28-29 0.2477803937756775 1105 30-31 0.25467814956839874 1105 32-33 0.12830075693995724 1105 34-35 0.12756785003029592 1105 36-37 0.028092291201566866 1105 38-39 -0.018435338340715646 1105 40-41 0.1408467411536023 1105 42-43 0.14605843799198226 1105 44-45 -0.02112872314319958 1105 46-47 0.21997276914791541 1105 48-49 -0.014591110409796215 1105 50-51 -0.035688895447364644 1105 52-53 -0.2858233293202517 1105 54-55 -0.32183633698397074 1105 56-57 -0.20819316403730426 1105 58-59 -0.20958802603450977 1105 60-61 -0.17707688334815685 1105 62-63 -0.14205115525744105 1105 64-65 -0.05706243058283178 1105 66-67 0.08250210337794428 1105 68-69 0.1056326154155407 1105 70-71 0.042206737652254134 1105 72-73 -0.16680963565409357 1105 74-75 -0.12881201422360178 1105 76-77 -0.09517455658093255 1105 78-79 -0.03434565817574864 1105 80-81 0.009711163370358378 1105 82-83 0.4080080549862828 1105 84-85 0.5553696024696428 1105 86-87 0.5344523441922462 1105 88-89 0.34260743624960455 1105 90-91 0.28588600121439 1105 92-93 0.15652159460850967 1105 94-95 0.12917736766764065 1105 96-97 0.46773240125936155 1105 98-99 0.808591327778462 1105 100-101 1.2453097443657235 1106 1 0.07160229015073583 1106 2 0.3508449324403138 1106 3 0.34691690431144906 1106 4 0.3467472981679478 1106 5 0.2737821065224324 1106 6 0.22747598495850951 1106 7 0.1731409960965422 1106 8 0.17300526887611767 1106 9 0.4169748223669245 1106 10-11 0.17007025056601321 1106 12-13 -0.058181343019349185 1106 14-15 0.14621468088789413 1106 16-17 0.19534448614968625 1106 18-19 0.0864714222406846 1106 20-21 0.345543676426324 1106 22-23 0.012236312117266834 1106 24-25 0.15085294062684795 1106 26-27 0.21179330478001646 1106 28-29 0.4031736857753785 1106 30-31 0.4337874234203909 1106 32-33 0.6445129791606163 1106 34-35 0.3682237383815661 1106 36-37 0.381217894610117 1106 38-39 0.44493344663936085 1106 40-41 0.26987081548085 1106 42-43 0.2658131000056798 1106 44-45 0.2698706412077456 1106 46-47 0.26074134074158195 1106 48-49 0.5303778893661075 1106 50-51 0.6292172864159156 1106 52-53 0.496125787747701 1106 54-55 0.17306508103492035 1106 56-57 0.2713082108532845 1106 58-59 0.08410474264701406 1106 60-61 0.04139365481122326 1106 62-63 0.123992993459062 1106 64-65 -0.010900355576247023 1106 66-67 0.27335538119886493 1106 68-69 0.3645722231171824 1106 70-71 0.2391811326933464 1106 72-73 0.3359922941257132 1106 74-75 0.3219561909205062 1106 76-77 0.47553083065055546 1106 78-79 0.4596424547640119 1106 80-81 0.5106935300989939 1106 82-83 0.2654796093385059 1106 84-85 0.2127336179676398 1106 86-87 0.14899179460245904 1106 88-89 0.360752679945616 1106 90-91 0.6484695270511978 1106 92-93 0.5956106291689309 1106 94-95 0.6820870477148837 1106 96-97 0.6921115863600882 1106 98-99 0.8788185687937542 1106 100-101 0.8348696666220405 1107 1 0.36480535172657014 1107 2 0.46729979694062607 1107 3 0.5277558367400843 1107 4 0.5125800234189981 1107 5 0.38824056581566424 1107 6 0.48939834627626055 1107 7 0.39148044184138087 1107 8 0.36270956714916736 1107 9 0.44350932195469284 1107 10-11 0.473053170561343 1107 12-13 0.5787011375772977 1107 14-15 0.6323369796600389 1107 16-17 0.7068522459113851 1107 18-19 0.6678896912436585 1107 20-21 0.6559436208302287 1107 22-23 0.7690531514161236 1107 24-25 0.5831923358297928 1107 26-27 0.5808344932728886 1107 28-29 0.5424253181209338 1107 30-31 0.4095588982015599 1107 32-33 0.5600227100565647 1107 34-35 0.49660017448238847 1107 36-37 0.5647355479495175 1107 38-39 0.5832461051750997 1107 40-41 0.62925376201067 1107 42-43 0.6114747093230761 1107 44-45 0.38833375667388026 1107 46-47 0.17211295324944587 1107 48-49 0.16753677100012254 1107 50-51 0.33983487689382486 1107 52-53 0.18555210554887935 1107 54-55 0.2562467886563198 1107 56-57 0.3235101004887255 1107 58-59 0.4066307954190833 1107 60-61 0.4264510988439625 1107 62-63 0.43875770510171463 1107 64-65 0.4972305732260054 1107 66-67 0.7116516136158033 1107 68-69 0.7671353294226044 1107 70-71 0.7855272689227313 1107 72-73 0.9380450851444202 1107 74-75 0.8137644538900339 1107 76-77 0.6503742297199544 1107 78-79 0.7873458938246891 1107 80-81 0.9076014711797704 1107 82-83 1.0339487266557406 1107 84-85 0.9854371991774258 1107 86-87 0.7996627383777479 1107 88-89 1.0645518516901653 1107 90-91 0.8569722547345755 1107 92-93 0.892413221968603 1107 94-95 0.9114462079611485 1107 96-97 1.0755398437068244 1107 98-99 1.3411253644686312 1107 100-101 1.5348489760300978 1108 1 0.06357673426296984 1108 2 0.07059566811626539 1108 3 0.1310609236986764 1108 4 0.1835059177723366 1108 5 0.10703938066598084 1108 6 0.005585101045816998 1108 7 0.11959794921784805 1108 8 0.2040767592690571 1108 9 0.16018850672652718 1108 10-11 0.21585526008652778 1108 12-13 0.15517255252253648 1108 14-15 0.20163429156974644 1108 16-17 0.34957285255564585 1108 18-19 0.3496191711509411 1108 20-21 0.25924141196065875 1108 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0.1519976138267367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4761.0 3 1008.0 4 954.0 5 1047.0 6 1140.0 7 1353.0 8 1738.0 9 2136.0 10 2706.0 11 3772.0 12 5119.0 13 7055.0 14 8055.0 15 10115.0 16 13805.0 17 18298.0 18 15661.0 19 9330.0 20 8446.0 21 8703.0 22 9228.0 23 9910.0 24 11181.0 25 12820.0 26 14564.0 27 16789.0 28 20017.0 29 23815.0 30 28861.0 31 35599.0 32 44771.0 33 57414.0 34 77182.0 35 106397.0 36 143309.0 37 185387.0 38 138854.0 39 16485.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.04185902781914 19.028396229224995 14.71981667361649 20.20992806933937 2 14.899664680553332 21.14540805883163 41.50769076178177 22.447236498833266 3 19.51606165845962 27.87264545455391 30.153160000707054 22.45813288627942 4 11.762445583861096 17.11683548578877 38.23815916912522 32.88255976122491 5 12.35577348027745 39.30534944198142 33.26888633540662 15.069990742334513 6 28.55037535820349 36.859394554749116 18.89175885583158 15.69847123121582 7 26.953074519407327 35.33882050838649 20.55199010039987 17.156114871806313 8 26.482214908610846 33.764991353823845 20.11900113422956 19.63379260333575 9 27.975669126903984 16.80455525120269 20.925499253374692 34.29427636851863 10-11 24.514958384491685 27.712028027880976 26.518909103250067 21.254104484377272 12-13 26.67099621033214 25.66718980694636 27.56843902130619 20.093374961415307 14-15 23.63088383717465 25.626196804245016 28.181703649535272 22.56121570904506 16-17 20.4284866308843 29.510318922308386 28.618920588526493 21.44227385828082 18-19 20.23189340879964 27.218120466873398 32.82212595764224 19.727860166684717 20-21 21.911695260617687 25.923168165153303 32.43661178412868 19.728524790100323 22-23 21.129385908846444 26.177140910141016 32.144110541892715 20.549362639119824 24-25 20.693572618551794 26.245760694948533 32.81872880347475 20.24193788302493 26-27 20.805604040338306 26.185129491169313 32.848154957107425 20.16111151138495 28-29 20.107845958519427 26.285348746717276 33.43945298789278 20.16735230687052 30-31 20.19884682416416 26.783794174430515 33.10631801452557 19.911040986879755 32-33 19.930367886104214 26.796276555437217 32.653965399654766 20.619390158803803 34-35 20.530606078214888 26.442303445871346 32.65170550293451 20.375384972979255 36-37 20.840276373676176 26.330100607269102 32.62938457355207 20.200238445502652 38-39 20.919032948156605 26.338308268108285 32.685288938318585 20.057369845416524 40-41 20.42734303908419 26.60279200674439 32.84660507855598 20.12325987561544 42-43 20.208718642120317 27.003648299977733 32.259914762995294 20.52771829490665 44-45 20.321754922052683 27.392907558791084 31.52344833965406 20.761889179502173 46-47 20.547789204578468 27.263308349311487 30.78361512985881 21.405287316251233 48-49 20.85975305588576 27.493371727646466 30.515017807888654 21.131857408579123 50-51 21.259475749936428 27.887422024229842 30.154582504512707 20.698519721321023 52-53 20.94506882205579 28.141813792680182 30.16472286127162 20.748394523992406 54-55 20.446724772898094 28.06994873444405 30.354331495569987 21.128994997087872 56-57 20.95086811179237 28.54635250836649 29.51203654346554 20.9907428363756 58-59 20.896615603979978 27.85372657225623 29.9470318157672 21.302626007996583 60-61 21.035799327473043 27.804426260895106 30.231880528876093 20.927893882755765 62-63 21.5032640555152 27.62171253353633 30.165802959515673 20.7092204514328 64-65 21.10993534199313 27.613704541768385 30.449910609015873 20.826449507222613 66-67 20.727432932457038 28.061154780261603 30.18706321167625 21.0243490756051 68-69 20.756018249357382 28.332698965073494 29.994557910386305 20.916724875182815 70-71 20.7628341541423 28.357853810336664 29.608284276113412 21.271027759407623 72-73 20.873029741400945 28.297226255119096 29.293207197125316 21.536536806354647 74-75 20.73825462803514 28.447734541416786 29.586331813003632 21.227679017544446 76-77 21.083007725810194 28.030241413558816 29.713485519508797 21.173265341122196 78-79 20.827740450578748 28.121944055059995 29.827835266208822 21.222480228152435 80-81 20.564371832158916 28.92477675811985 29.436232340723762 21.07461906899747 82-83 20.73288201780697 28.811860531142212 29.013665390536314 21.44159206051451 84-85 20.715676739646323 28.28809220556481 29.089231206736237 21.90699984805263 86-87 21.001374081486706 28.265046703435853 29.316449061168232 21.41713015390921 88-89 21.40786216466278 27.68315085127057 29.41283079472522 21.496156189341427 90-91 20.775821524241866 28.123158460254345 29.693567786322834 21.407452229180958 92-93 20.77936200557942 28.40809968413035 29.466079447467443 21.346458862822786 94-95 20.4509882649922 28.38234228004528 29.212975613564502 21.953693841398014 96-97 20.638566232819862 28.092099576253464 28.957345575106498 22.31198861582018 98-99 20.76032850430151 28.002435430871408 28.949232420976312 22.28800364385077 100-101 21.22346004478839 27.699234675265593 29.4611684481181 21.616136831827916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1995.0 1 1316.5 2 1170.5 3 1515.5 4 1058.5 5 881.0 6 1371.0 7 2761.5 8 4331.0 9 4780.0 10 3969.5 11 3165.0 12 3094.5 13 3217.5 14 3365.0 15 3406.0 16 3435.5 17 3492.5 18 3569.5 19 3713.0 20 3886.0 21 4289.0 22 4817.5 23 5467.5 24 6252.0 25 7191.0 26 8639.0 27 10261.5 28 11342.0 29 12772.0 30 14736.0 31 16238.5 32 18047.0 33 20793.0 34 23780.5 35 26000.0 36 28534.0 37 31455.0 38 32760.0 39 33064.0 40 33209.5 41 33864.0 42 34813.0 43 36032.5 44 37386.5 45 37736.5 46 36922.5 47 38169.0 48 48527.5 49 48849.0 50 39167.0 51 34824.5 52 30164.0 53 27776.5 54 26345.0 55 23922.5 56 21275.0 57 19012.0 58 17273.0 59 15552.5 60 13614.5 61 11515.5 62 9275.0 63 6862.0 64 4924.0 65 3799.0 66 2814.5 67 2065.5 68 1595.0 69 1189.5 70 895.5 71 718.0 72 583.0 73 529.0 74 474.5 75 369.0 76 255.5 77 164.0 78 111.5 79 81.5 80 50.0 81 37.0 82 28.5 83 20.0 84 13.0 85 8.0 86 6.5 87 7.0 88 4.5 89 3.5 90 3.5 91 2.0 92 2.0 93 3.0 94 3.0 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3173165780283321 2 0.2776056144622134 3 0.2689768303228464 4 0.2754716140836602 5 0.2786262233389126 6 0.21172995060253036 7 0.20310116646316345 8 0.2011527313349193 9 0.21172995060253036 10-11 0.21224025504088004 12-13 0.2070908193448062 14-15 0.21038460253779037 16-17 0.19655999138977237 18-19 0.20082799214687863 20-21 0.18890542481452754 22-23 0.1986939917683255 24-25 0.19855481783059378 26-27 0.19813729601739857 28-29 0.21084851566356277 30-31 0.20750834115800137 32-33 0.21140521141448965 34-35 0.20648773228130207 36-37 0.1921064253823572 38-39 0.18199311924051853 40-41 0.1791632491733068 42-43 0.19312703425905653 44-45 0.1942404257609103 46-47 0.1948899041369917 48-49 0.2116835592899531 50-51 0.2053743407794483 52-53 0.2232349961216863 54-55 0.19618886088915446 56-57 0.17800346635887576 58-59 0.1812508582392827 60-61 0.17127672603517574 62-63 0.17651894435640406 64-65 0.17596224860547716 66-67 0.1758230746677454 68-69 0.17763233585825783 70-71 0.17800346635887576 72-73 0.1775859445456806 74-75 0.1887662508767958 76-77 0.18357042386814476 78-79 0.18704977231143788 80-81 0.1797199449242337 82-83 0.16223042008261365 84-85 0.16306546370900402 86-87 0.16668398609002882 88-89 0.17048807372136265 90-91 0.17832820554691647 92-93 0.17345711772630612 94-95 0.17276124803764747 96-97 0.17712203141990818 98-99 0.18584359818442958 100-101 0.17067363897167162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1077788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.098895085646134 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35088973587007 43.72146348509381 2 12.062479096710515 13.292585404449047 3 3.5905859667519135 5.935119584341711 4 1.5880063710269132 3.4998958573019814 5 0.8504112802813392 2.342836095593576 6 0.5023339791867982 1.660682833030113 7 0.3442532074297227 1.327757995134723 8 0.239163488447679 1.0542115166636647 9 0.17950333386822992 0.8901391824296381 >10 1.1372012766331752 11.844970041265878 >50 0.10289604078466516 3.9436837808085294 >100 0.04721723434375767 4.808067486657931 >500 0.002529494332636179 1.0163960389841051 >1k 0.0020235954661089435 2.2320016133537046 >5k 3.3726591101815724E-4 1.3894493433557282 >10k+ 1.6863295550907862E-4 1.0407397415358774 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11201 1.0392581843553648 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8724 0.8094356218477103 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 0.5780357547124295 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4287 0.39775911403726893 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3596 0.33364632005552114 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2377 0.22054429999220623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1999 0.18547246768381168 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1987 0.18435907618195785 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1604 0.14882333074779083 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1556 0.14436976474037566 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1499 0.13908115510657013 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1383 0.1283183705886501 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1321 0.12256584782907214 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1289 0.11959680382412868 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1124 0.10428767067363898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.639131257724153E-5 0.0 12-13 0.0 0.0 0.0 9.278262515448306E-5 0.0 14-15 0.0 0.0 0.0 9.278262515448306E-5 0.0 16-17 0.0 0.0 0.0 9.278262515448306E-5 0.0 18-19 0.0 0.0 0.0 9.278262515448306E-5 0.0 20-21 0.0 0.0 0.0 1.391739377317246E-4 0.0 22-23 0.0 0.0 0.0 3.2473918804069076E-4 0.0 24-25 0.0 0.0 0.0 6.494783760813815E-4 0.0 26-27 0.0 0.0 0.0 0.0010670001892765554 0.0 28-29 0.0 0.0 0.0 0.0017628698779351784 0.0 30-31 0.0 0.0 0.0 0.0033865658181386326 0.0 32-33 0.0 0.0 0.0 0.00575252275957795 0.0 34-35 0.0 0.0 0.0 0.007747349200399337 0.0 36-37 0.0 0.0 0.0 0.010716393205342796 0.0 38-39 0.0 0.0 0.0 0.01595861152657109 0.0 40-41 0.0 0.0 0.0 0.021989482161612485 0.0 42-43 0.0 0.0 0.0 0.027370874420572504 0.0 44-45 0.0 0.0 0.0 0.030154353175206997 0.0 46-47 0.0 0.0 0.0 0.03511822362097185 0.0 48-49 0.0 0.0 0.0 0.041984137882403585 0.0 50-51 0.0 0.0 0.0 0.051494356960738105 0.0 52-53 0.0 0.0 0.0 0.06717462061184575 0.0 54-55 0.0 0.0 0.0 0.08076727519697752 0.0 56-57 0.0 0.0 0.0 0.10841649749301346 0.0 58-59 0.0 0.0 0.0 0.1555964623840681 9.278262515448306E-5 60-61 0.0 0.0 0.0 0.20514238421656206 9.278262515448306E-5 62-63 0.0 0.0 0.0 0.23794104220867185 9.278262515448306E-5 64-65 0.0 0.0 0.0 0.28720861616570237 9.278262515448306E-5 66-67 0.0 0.0 0.0 0.36004297691197157 9.278262515448306E-5 68-69 0.0 0.0 0.0 0.4583925595757236 9.278262515448306E-5 70-71 0.0 0.0 0.0 0.5620771431858584 9.278262515448306E-5 72-73 0.0 0.0 0.0 0.6739265978095879 9.278262515448306E-5 74-75 0.0 0.0 0.0 0.7921316622563992 9.278262515448306E-5 76-77 0.0 0.0 0.0 0.9020790730644617 9.278262515448306E-5 78-79 0.0 0.0 0.0 1.0383303581038201 9.278262515448306E-5 80-81 0.0 0.0 0.0 1.2049679528812716 9.278262515448306E-5 82-83 0.0 0.0 0.0 1.3690076341543977 9.278262515448306E-5 84-85 0.0 0.0 0.0 1.5617635379128363 9.278262515448306E-5 86-87 0.0 0.0 0.0 1.7954365793644018 9.278262515448306E-5 88-89 0.0 0.0 0.0 2.0607948873062236 9.278262515448306E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 105 6.8728434E-5 27.161722 4 GTACCTG 1065 0.0 25.891342 1 TACCTGG 1070 0.0 25.322504 2 GTACATG 9950 0.0 25.03712 1 CATGGGG 2650 0.0 24.75304 4 TACATGG 10055 0.0 24.630384 2 TAGCCCT 520 0.0 23.766506 4 ACATGGG 10140 0.0 23.480877 3 GAGTACT 7865 0.0 23.100695 12-13 GTATCAA 14865 0.0 22.995358 1 ATAACGC 125 2.2480154E-4 22.811602 3 GGTATCA 11225 0.0 22.65915 1 GTGTAGC 475 0.0 22.019388 1 AGTACTT 7945 0.0 21.911514 12-13 GTACTTT 8405 0.0 21.531765 14-15 ACCTGGG 1290 0.0 20.99905 3 ATAGCCC 325 2.1827873E-11 20.47195 3 CATGGGA 7245 0.0 20.404095 4 GGACCGA 145 6.1361905E-4 19.659685 6 ATGGGAG 2230 0.0 19.610031 5 >>END_MODULE