FastQCFastQC Report
Thu 26 May 2016
SRR937534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077788
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127791.1856691668491393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107160.9942586111554406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77090.7152612573159101No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43930.40759407230364414No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40510.3758624145008109No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28290.2624820465620326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23810.2209154304928242No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23100.21432786410685592No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20450.18974046844091788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.18807038118813718No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14450.13407089334822803No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.1325863713457563No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12570.11662775981918522No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11960.11096801968476175No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.10122584404354104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG11250.034.209891
TACCTGG11300.033.6349222
TATCACG851.6299166E-533.5359952
ATTGCCG851.6319886E-533.5297748
GTACCGT700.00839470127.1406296
ACCTGGG14950.024.7874763
GAGTACT93850.023.18085712-13
CATGGGG25450.022.9612854
GTACATG101950.022.8830281
GGTATCA129450.022.793381
GTATCAA169550.022.6149121
TACATGG102800.022.599282
AGTACTT95450.022.36928212-13
ACATGGG101650.022.0136663
GTGTAGC4000.021.3811821
GTACTTT102500.021.06046914-15
ATGGGAG23100.020.5668095
GTATAGG2551.1876182E-820.4961221
TACTTTT110300.019.31278614-15
CATGGGA71800.019.2551734
Produced by FastQC (version 0.11.5)