##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937534_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1077788 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14437162039288 33.0 31.0 34.0 31.0 34.0 2 32.34843308702639 34.0 31.0 34.0 31.0 34.0 3 32.41189083567455 34.0 31.0 34.0 31.0 34.0 4 36.06223301799612 37.0 35.0 37.0 35.0 37.0 5 35.99349129884541 37.0 35.0 37.0 35.0 37.0 6 36.04826552160536 37.0 35.0 37.0 35.0 37.0 7 35.99572364880663 37.0 35.0 37.0 35.0 37.0 8 35.99259872999143 37.0 35.0 37.0 35.0 37.0 9 37.63072607971141 39.0 37.0 39.0 35.0 39.0 10-11 37.568689760880616 39.0 37.0 39.0 35.0 39.0 12-13 37.457007314982164 39.0 37.0 39.0 35.0 39.0 14-15 38.555166693264354 40.0 38.0 41.0 34.0 41.0 16-17 38.52573186934722 40.0 38.0 41.0 34.0 41.0 18-19 38.619238198977904 40.0 38.0 41.0 34.0 41.0 20-21 38.55897542002694 40.0 38.0 41.0 34.0 41.0 22-23 38.3928527688191 40.0 38.0 41.0 34.0 41.0 24-25 38.441257464362195 40.0 38.0 41.0 34.0 41.0 26-27 38.26887569726143 40.0 38.0 41.0 34.0 41.0 28-29 38.10113306141839 40.0 37.0 41.0 33.5 41.0 30-31 38.03173768867347 40.0 37.0 41.0 34.0 41.0 32-33 37.846721247592285 40.0 36.5 41.0 33.0 41.0 34-35 37.66876185298037 40.0 36.0 41.0 33.0 41.0 36-37 37.44774760899175 40.0 36.0 41.0 33.0 41.0 38-39 37.263423790207355 40.0 35.5 41.0 33.0 41.0 40-41 37.018652091134804 39.5 35.0 41.0 32.0 41.0 42-43 36.732369445568146 39.0 35.0 41.0 31.0 41.0 44-45 36.28133454816717 39.0 35.0 41.0 30.5 41.0 46-47 35.70280333423642 39.0 35.0 41.0 28.5 41.0 48-49 35.18253172237954 39.0 35.0 40.5 25.5 41.0 50-51 34.854726532490616 39.0 35.0 40.0 24.0 41.0 52-53 34.560478498554446 38.5 35.0 40.0 23.0 41.0 54-55 34.333359621743796 38.0 34.0 40.0 22.5 41.0 56-57 34.084324097132274 38.0 34.0 40.0 21.5 41.0 58-59 33.82816379473515 38.0 34.0 40.0 20.0 41.0 60-61 33.46379900314348 37.5 33.0 40.0 18.5 41.0 62-63 33.09958544723081 37.0 33.0 40.0 17.5 41.0 64-65 32.68905619658041 36.5 32.5 39.0 13.0 41.0 66-67 32.25224255604998 36.0 32.0 39.0 8.0 40.0 68-69 31.829319866244568 35.0 31.0 39.0 7.5 40.0 70-71 31.41285159975802 35.0 31.0 38.0 6.0 40.0 72-73 30.905499040627657 35.0 30.5 37.5 2.0 39.5 74-75 30.392830500989064 34.5 30.0 37.0 2.0 39.0 76-77 29.179275516149744 33.5 28.5 35.5 2.0 38.0 78-79 29.745872101006878 34.0 30.0 36.0 2.0 38.0 80-81 29.54548389850323 34.0 29.5 35.5 2.0 37.0 82-83 29.17804011549581 34.0 29.0 35.0 2.0 37.0 84-85 28.779908479218548 34.0 29.0 35.0 2.0 36.5 86-87 28.321259839597396 34.0 29.0 35.0 2.0 36.0 88-89 27.921034099470397 34.0 27.5 35.0 2.0 36.0 90-91 27.35472189335936 33.0 26.5 35.0 2.0 35.0 92-93 26.86984499734642 33.0 25.5 35.0 2.0 35.0 94-95 26.38463130040416 33.0 25.0 35.0 2.0 35.0 96-97 25.78235098182574 32.0 23.5 34.5 2.0 35.0 98-99 24.86991922344654 32.0 17.5 34.0 2.0 35.0 100-101 22.317571730247508 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2385187371500166 1101 2 0.27082104814442687 1101 3 0.15820828513074758 1101 4 0.15809850672394532 1101 5 0.14874312397382994 1101 6 0.15881243918734356 1101 7 0.19622871071203463 1101 8 0.11318291518691836 1101 9 0.14661703646667235 1101 10-11 0.2666700660325958 1101 12-13 0.29277264816246173 1101 14-15 0.3840306555793589 1101 16-17 0.26337061624500535 1101 18-19 0.27654422768444675 1101 20-21 0.2550803088216753 1101 22-23 0.2755721429442062 1101 24-25 0.2529696467182987 1101 26-27 0.2283904405372752 1101 28-29 0.30613232563122494 1101 30-31 0.2954222953179553 1101 32-33 0.32668499603834533 1101 34-35 0.2551161354599998 1101 36-37 0.22779939915220382 1101 38-39 0.32078261996719704 1101 40-41 0.20391129154652532 1101 42-43 0.35110293432018125 1101 44-45 0.3271028353905976 1101 46-47 0.3726996028218821 1101 48-49 0.5695315682669886 1101 50-51 0.5053112112980145 1101 52-53 0.3115580698349021 1101 54-55 0.4543695696137817 1101 56-57 0.5455440367807967 1101 58-59 0.5017081765170133 1101 60-61 0.45814907581432607 1101 62-63 0.4237015680669316 1101 64-65 0.5339372899957127 1101 66-67 0.4207143436494505 1101 68-69 0.2725206801776352 1101 70-71 0.32435526489518196 1101 72-73 0.3905115657500673 1101 74-75 0.4357730659376742 1101 76-77 0.39747119312565005 1101 78-79 0.4907124120804802 1101 80-81 0.4907797334275479 1101 82-83 0.4953557491215079 1101 84-85 0.5941180931540764 1101 86-87 0.5439186134004075 1101 88-89 0.9273755949154321 1101 90-91 1.1166454482229007 1101 92-93 1.0781861686387053 1101 94-95 1.2322144174580494 1101 96-97 1.0042058570373271 1101 98-99 1.0312382048673285 1101 100-101 1.3526478203575074 1102 1 0.20910067047883985 1102 2 0.12883403397000848 1102 3 0.13917762264147626 1102 4 0.08236670178072814 1102 5 0.048189170960377226 1102 6 0.09137911141424837 1102 7 0.07001927222371052 1102 8 0.04925951522614014 1102 9 0.14666310034331076 1102 10-11 0.12251351306228031 1102 12-13 0.11535867419691925 1102 14-15 -0.021822457885036783 1102 16-17 0.13769440202563743 1102 18-19 0.0921051635628416 1102 20-21 0.02897926003095108 1102 22-23 0.14271589643825422 1102 24-25 0.17711500477072661 1102 26-27 0.15483654964025817 1102 28-29 0.04889907560836093 1102 30-31 0.1830232956020197 1102 32-33 0.1444601387142228 1102 34-35 0.15726808623028177 1102 36-37 0.027706294286389266 1102 38-39 0.07953039441891718 1102 40-41 0.19993130281123683 1102 42-43 0.30590275536900435 1102 44-45 0.4425256494328167 1102 46-47 0.40195609695607004 1102 48-49 0.25831773626194376 1102 50-51 0.42620348254724405 1102 52-53 0.42482530383171735 1102 54-55 0.43001783143527206 1102 56-57 0.32266571451093284 1102 58-59 0.3532292964554813 1102 60-61 0.24117007047301087 1102 62-63 0.21373788175634445 1102 64-65 0.27496722244234206 1102 66-67 0.1816243285530561 1102 68-69 0.2546241661661881 1102 70-71 0.4188943923197286 1102 72-73 0.46118332363833403 1102 74-75 0.3657462023223452 1102 76-77 0.3291110043195893 1102 78-79 0.5093225672110542 1102 80-81 0.3509835451435457 1102 82-83 0.45788627516400027 1102 84-85 0.43515549353222127 1102 86-87 0.5237154158694288 1102 88-89 0.43001693947811503 1102 90-91 0.7177633347564054 1102 92-93 0.6221708893904108 1102 94-95 0.6755694589748593 1102 96-97 0.739088355765336 1102 98-99 0.6667487077801724 1102 100-101 0.87089783972527 1103 1 0.14139331285025492 1103 2 0.1465105564796474 1103 3 0.1594918810466055 1103 4 0.08863491398651746 1103 5 0.00987909956784705 1103 6 6.965134973597742E-4 1103 7 0.056362938170664734 1103 8 0.026469559133708742 1103 9 0.16707239381570105 1103 10-11 0.007388784664293269 1103 12-13 0.0894561487254748 1103 14-15 0.07459642934320954 1103 16-17 0.19284584963534002 1103 18-19 -0.055236452634964905 1103 20-21 -0.046360986020985706 1103 22-23 0.0038488101587219603 1103 24-25 -0.015549450336493464 1103 26-27 0.07242029331085575 1103 28-29 -0.00973414236105441 1103 30-31 0.2179141556898898 1103 32-33 0.17827108593352392 1103 34-35 0.1346346987018805 1103 36-37 0.093192634533807 1103 38-39 0.024172467742232584 1103 40-41 0.12644424057079107 1103 42-43 0.23911224684250953 1103 44-45 0.4219758544761092 1103 46-47 0.4895353555184556 1103 48-49 0.6520104161004312 1103 50-51 0.577332827324895 1103 52-53 0.5687261720009857 1103 54-55 0.4718339447334756 1103 56-57 0.4913888534303297 1103 58-59 0.6620754886943345 1103 60-61 0.5386342750705211 1103 62-63 0.5880232385617461 1103 64-65 0.49002234961840685 1103 66-67 0.6980756507711661 1103 68-69 0.4074884137004062 1103 70-71 0.4813057669917207 1103 72-73 0.18763787294648893 1103 74-75 0.3915796735362882 1103 76-77 0.4811623259907307 1103 78-79 0.5728343659172062 1103 80-81 0.4306677142157156 1103 82-83 0.4614576109468196 1103 84-85 0.4657085839495423 1103 86-87 0.4998281124176742 1103 88-89 0.32722489124678944 1103 90-91 0.5388680251130111 1103 92-93 0.6615013912712833 1103 94-95 0.8750504581147602 1103 96-97 0.8966028263926624 1103 98-99 1.050905746733612 1103 100-101 1.3224880703938418 1104 1 0.21851118530369007 1104 2 0.1713787229503012 1104 3 0.07879702485761442 1104 4 0.07535807170440023 1104 5 0.09277163288528101 1104 6 0.1010200679352593 1104 7 0.039636474452663606 1104 8 0.13454730975291085 1104 9 0.2460600532218109 1104 10-11 0.24636652607005516 1104 12-13 0.23530427324786984 1104 14-15 0.3310871644133684 1104 16-17 0.2623111447635935 1104 18-19 0.2282788010327934 1104 20-21 0.22073808016946828 1104 22-23 0.2495123948383906 1104 24-25 0.04693598429413015 1104 26-27 0.1998137561020954 1104 28-29 0.040210170505787346 1104 30-31 0.11443973925310758 1104 32-33 0.31388212688998607 1104 34-35 0.25783604494849044 1104 36-37 0.25771356259849654 1104 38-39 0.3068646086753901 1104 40-41 0.29929588246051964 1104 42-43 0.14785038453470634 1104 44-45 0.512931505297459 1104 46-47 0.5557136193806258 1104 48-49 0.12755007997235168 1104 50-51 0.20756687587432054 1104 52-53 0.31073960736868145 1104 54-55 0.2427992696699306 1104 56-57 0.2102223502571121 1104 58-59 0.318247411539879 1104 60-61 0.3579173337167916 1104 62-63 0.45775057484699033 1104 64-65 0.4267273514339962 1104 66-67 0.249639090745589 1104 68-69 0.32672039193499813 1104 70-71 0.18177267176266199 1104 72-73 0.3337285278054978 1104 74-75 0.20120352117845997 1104 76-77 0.185538169427236 1104 78-79 0.4054647225858439 1104 80-81 0.3445308295517613 1104 82-83 0.3724428802774362 1104 84-85 0.3477961604346689 1104 86-87 0.3183483521839747 1104 88-89 0.3144236252113366 1104 90-91 0.4033044062894078 1104 92-93 0.41057598222151626 1104 94-95 0.30667790441935594 1104 96-97 0.6837485830602148 1104 98-99 0.7432925975246114 1104 100-101 0.9951086987506521 1105 1 0.11953065750945768 1105 2 0.14738720740502487 1105 3 0.14457407042197445 1105 4 0.08718321222477243 1105 5 -0.04688404785025568 1105 6 0.07080579996517145 1105 7 0.1133492696546341 1105 8 0.07827842242308236 1105 9 0.1802161256945638 1105 10-11 0.0797000868930553 1105 12-13 0.18045526269143153 1105 14-15 0.20931046554326116 1105 16-17 0.14698889654688685 1105 18-19 0.1812867149434254 1105 20-21 0.12810997391177636 1105 22-23 0.13177181768799784 1105 24-25 0.09519575656787538 1105 26-27 0.07185764158925423 1105 28-29 0.21143925624300408 1105 30-31 0.17519551908313957 1105 32-33 0.21229593241907452 1105 34-35 0.30315676443462536 1105 36-37 0.3477025611980764 1105 38-39 0.3139032528005643 1105 40-41 0.3382549670784911 1105 42-43 0.295102627810337 1105 44-45 -0.04873844177024722 1105 46-47 -0.033878864724670166 1105 48-49 0.15297955771976746 1105 50-51 0.20190204186443594 1105 52-53 0.053747371136132926 1105 54-55 -0.09607969308637365 1105 56-57 -0.07583009885117775 1105 58-59 0.036030779497139065 1105 60-61 -0.08933497780573418 1105 62-63 -0.18067529673417937 1105 64-65 -0.14116330065149185 1105 66-67 0.12939862961492565 1105 68-69 -0.06379724715900892 1105 70-71 -0.056557108182033744 1105 72-73 0.19690202234711052 1105 74-75 -0.0345768957828021 1105 76-77 -0.21747752463009462 1105 78-79 0.18881937170122853 1105 80-81 0.20323142220784973 1105 82-83 0.34737067505627905 1105 84-85 0.3959132966855101 1105 86-87 0.468902039291045 1105 88-89 0.3038733367999349 1105 90-91 0.3004849903190845 1105 92-93 0.39458095605565546 1105 94-95 0.3703860451826877 1105 96-97 0.5038528233766648 1105 98-99 0.5276818696577621 1105 100-101 0.6003515621722215 1106 1 0.1336354423542332 1106 2 0.1689055489106508 1106 3 0.12115582884432285 1106 4 0.13685263243107926 1106 5 0.07545514068945636 1106 6 0.02484174866786759 1106 7 0.04036731447705222 1106 8 -0.005826686588619623 1106 9 0.14703764079440163 1106 10-11 0.16034964694215859 1106 12-13 0.0029548433885651093 1106 14-15 0.2669748287455249 1106 16-17 0.31143983529202757 1106 18-19 0.11185156876528168 1106 20-21 0.08217622517496181 1106 22-23 0.07778992029670206 1106 24-25 0.13683429353206122 1106 26-27 0.16398834851275268 1106 28-29 0.3251316443807468 1106 30-31 0.09948316226274301 1106 32-33 0.17131418583586822 1106 34-35 0.10399635677357111 1106 36-37 0.07568880806387313 1106 38-39 0.08364854216183204 1106 40-41 0.27309177721955535 1106 42-43 0.16844497204461106 1106 44-45 0.06686265532338354 1106 46-47 0.24728203341503274 1106 48-49 0.42633543239964666 1106 50-51 0.45941249624780056 1106 52-53 0.4693596731300431 1106 54-55 0.532434875398117 1106 56-57 0.4556229018774971 1106 58-59 0.2944573578024787 1106 60-61 0.4523645166348018 1106 62-63 0.39073268707439013 1106 64-65 0.570623056431053 1106 66-67 0.5155145601642772 1106 68-69 0.5322474230434295 1106 70-71 0.5591838739873758 1106 72-73 0.5085324974207879 1106 74-75 0.5453387080626619 1106 76-77 0.6307066472854892 1106 78-79 0.6368924107379748 1106 80-81 0.5430749905246195 1106 82-83 0.35228779402976684 1106 84-85 0.3617013535302931 1106 86-87 0.5290617831146065 1106 88-89 0.18603654498134503 1106 90-91 0.3429723513325129 1106 92-93 0.5800710330241579 1106 94-95 0.7665507980899697 1106 96-97 0.8071130970017357 1106 98-99 1.05983394340997 1106 100-101 0.7712294625853211 1107 1 0.2870901394344898 1107 2 0.3134190672905319 1107 3 0.20402875702112055 1107 4 0.06578439770184019 1107 5 -4.856494700362646E-4 1107 6 0.19316840099327237 1107 7 0.16914492694068173 1107 8 0.19571389382197424 1107 9 0.18993192292562355 1107 10-11 0.2825473352818264 1107 12-13 0.26194735371016264 1107 14-15 0.32526938696189944 1107 16-17 0.3602688425542624 1107 18-19 0.3171614696083864 1107 20-21 0.2679793202348151 1107 22-23 0.32981791419376094 1107 24-25 0.3060454291770611 1107 26-27 0.3355812713826509 1107 28-29 0.3322469967999666 1107 30-31 0.23291299904398244 1107 32-33 0.3265883914592891 1107 34-35 0.2458777475604137 1107 36-37 0.004068560977138702 1107 38-39 0.08999313054540181 1107 40-41 0.11982582256195684 1107 42-43 0.3090059555900524 1107 44-45 0.11950994548761429 1107 46-47 0.07094369959207114 1107 48-49 0.08219481850505161 1107 50-51 0.09299711760997553 1107 52-53 -0.14997529017969669 1107 54-55 0.15511350154281445 1107 56-57 0.016274766084038106 1107 58-59 -0.06505747150803387 1107 60-61 0.20372519945591705 1107 62-63 -0.01719023020431365 1107 64-65 -0.018767408206983305 1107 66-67 -0.2043379838308681 1107 68-69 -0.17330255184356247 1107 70-71 -0.2146300496753959 1107 72-73 -0.09040327961360006 1107 74-75 -0.3189847095808851 1107 76-77 -0.191004417284951 1107 78-79 0.05513693246941287 1107 80-81 0.01859533522040735 1107 82-83 0.13225440478445094 1107 84-85 0.04446640633079113 1107 86-87 0.3971900584669008 1107 88-89 0.339539414813828 1107 90-91 0.3861975249913385 1107 92-93 0.4777584250303235 1107 94-95 0.680429762865419 1107 96-97 0.6619757019946455 1107 98-99 0.6002832609363224 1107 100-101 0.8897462788244361 1108 1 0.050633884452203404 1108 2 0.02991393640337492 1108 3 -0.05690922536751941 1108 4 0.009985903867971047 1108 5 -0.03202436267025632 1108 6 -0.02435018800892408 1108 7 -0.010311710496587523 1108 8 0.0024113104844118993 1108 9 0.03862182258110636 1108 10-11 -0.008867625313023098 1108 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68-69 -0.060587707927297174 2316 70-71 0.0010538045045720423 2316 72-73 -0.1788457623111661 2316 74-75 -0.1133815291673308 2316 76-77 -0.19221395625551807 2316 78-79 -0.07564792708218349 2316 80-81 -0.2998255531274445 2316 82-83 -0.2278718129126993 2316 84-85 -0.20761428446139263 2316 86-87 -0.07097601087905403 2316 88-89 -0.05886853394995839 2316 90-91 0.12160856960736766 2316 92-93 0.4367227759087129 2316 94-95 0.5475564090444145 2316 96-97 0.6433778875357952 2316 98-99 0.6357344579897273 2316 100-101 0.9501622183489076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 8.0 5 38.0 6 93.0 7 143.0 8 396.0 9 731.0 10 1211.0 11 2125.0 12 3489.0 13 5192.0 14 6515.0 15 9257.0 16 13506.0 17 19393.0 18 17553.0 19 9132.0 20 6719.0 21 6495.0 22 6645.0 23 7208.0 24 8155.0 25 9403.0 26 11169.0 27 13895.0 28 16865.0 29 20938.0 30 26342.0 31 32766.0 32 41473.0 33 54151.0 34 73737.0 35 105799.0 36 152080.0 37 217477.0 38 163440.0 39 14240.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.165352723088134 19.854762446115938 15.36669002222645 19.613194808569478 2 15.272298448303376 22.099151224545086 40.581914068443886 22.04663625870765 3 19.771420724669415 28.108681855800953 30.512958021429075 21.606939398100554 4 12.181639026671343 17.891677939776542 37.691248540990514 32.2354344925616 5 12.280159585278732 39.74829774774649 33.035618996179274 14.935923670795503 6 28.65303751758231 37.44641803397328 18.191518183538875 15.709026264905528 7 26.66786046977699 35.78727913096082 20.29805490504626 17.24680549421593 8 26.781148055090608 34.130738141452674 19.64904044209065 19.43907336136606 9 27.974796527703038 16.86760290520956 20.911811970443168 34.24578859664424 10-11 24.633972543765566 28.1334548167172 26.12243780780636 21.110134831710877 12-13 26.92542689452932 26.00188632940062 27.468175730187163 19.604511045882898 14-15 23.71291942385701 25.85313623829547 28.35297850783271 22.080965830014808 16-17 20.136566745964885 29.74072823226831 28.884390993405013 21.238314028361792 18-19 19.989877415595647 27.110201635200987 33.48761537519438 19.41230557400899 20-21 21.454219197096275 25.77533800710344 33.49239368038984 19.27804911541045 22-23 20.958483024301895 25.903759222695626 33.10646182509167 20.031295927910808 24-25 20.570511083812402 26.01072752712036 33.74782424744013 19.67093714162711 26-27 20.637327104494645 25.99893763352068 33.797913772027286 19.56582148995739 28-29 20.030664956356947 26.031625265882802 34.28328203177372 19.65442774598653 30-31 20.009361766878087 26.56663462573345 33.98223954989293 19.441764057495536 32-33 19.81994603762521 26.597067326784117 33.5360942968376 20.046892338753075 34-35 20.468032674329276 26.14475202915601 33.53377473120874 19.85344056530598 36-37 20.826266390050733 26.07419084272603 33.47021863297792 19.629324134245323 38-39 20.79787490675346 26.12392232980883 33.602155525947595 19.476047237490118 40-41 20.371403016205562 26.313906945586197 33.69010130101004 19.6245887371982 42-43 20.12212040366547 26.82190001159958 33.069017515369445 19.986962069365504 44-45 20.344910130749273 27.14759303313824 32.23992102342947 20.267575812683013 46-47 20.555016385411605 27.190551388584765 31.394300177771512 20.86013204823212 48-49 20.93138910435076 27.405714296318013 31.004381195559795 20.65851540377143 50-51 21.396740360813073 27.802360018853427 30.550442201991483 20.25045741834201 52-53 21.166927617920972 28.178374679609846 30.362571471649098 20.29212623082008 54-55 20.52379503204712 28.11605807450074 30.58198829454401 20.778158598908135 56-57 21.06040334462807 28.559002326988235 29.753114712726436 20.627479615657254 58-59 21.025053164444213 27.93081756337981 30.097894947800498 20.946234324375478 60-61 21.204309196242672 27.78440658088604 30.464247143222973 20.547037079648316 62-63 21.656485319933047 27.59160428581502 30.436134007801165 20.315776386450768 64-65 21.23047389653624 27.60222789639521 30.613673561034265 20.553624646034287 66-67 20.82802925992867 28.143011427108117 30.349846166407495 20.67911314655572 68-69 21.01336255367475 28.299906846244344 30.106662905877595 20.580067694203315 70-71 20.950548716445162 28.428735521271342 29.742537493458826 20.87817826882467 72-73 21.112779136527777 28.23732496557765 29.433153829881203 21.216742068013374 74-75 21.00542963922404 28.565636284686786 29.45389074660323 20.97504332948595 76-77 21.280808470682548 28.027636232728515 29.989478450307484 20.702076846281457 78-79 21.065413606386414 28.108496290550644 29.94341187691828 20.88267822614466 80-81 20.82578506967753 28.988023138163264 29.510575610290164 20.675616181869046 82-83 20.951161940532543 28.852459624816518 29.11601405128757 21.080364383363364 84-85 20.80928127031078 28.412895539781413 29.132613368083664 21.64520982182414 86-87 21.08935152367627 28.257922708361942 29.424061132616064 21.228664635345726 88-89 21.469574721559344 27.73843279012199 29.637414779158792 21.154577709159874 90-91 20.849436792627035 28.202311103522703 29.74364994010209 21.20460216374817 92-93 20.749275929114123 28.546369995931375 29.54097872393728 21.163375351017226 94-95 20.48426963373131 28.536177801200235 29.232001098546284 21.74755146652217 96-97 20.747447549982002 28.165928735521273 28.9427048733146 22.143918841182124 98-99 20.80102693858085 28.12102621486194 28.95989066277343 22.11805618378378 100-101 20.925179221863907 28.272788436999747 29.38895204510127 21.413080296035076 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1759.0 1 1236.0 2 1490.0 3 1924.5 4 1244.5 5 977.0 6 1467.5 7 2860.5 8 4270.5 9 4698.0 10 4069.0 11 3380.0 12 3339.0 13 3480.5 14 3678.5 15 3774.0 16 3814.0 17 3845.5 18 3935.5 19 4059.5 20 4240.5 21 4653.0 22 5160.0 23 5900.0 24 6703.5 25 7557.5 26 9027.5 27 10659.5 28 11700.5 29 13123.0 30 15013.5 31 16237.0 32 18019.0 33 20799.5 34 23718.0 35 25748.5 36 28427.0 37 31273.0 38 32581.0 39 32977.0 40 32823.5 41 33657.0 42 34542.5 43 35463.5 44 37236.5 45 37529.5 46 36325.5 47 37841.0 48 49820.0 49 49763.0 50 38656.0 51 34488.0 52 29795.5 53 27375.5 54 25699.0 55 23463.0 56 20904.0 57 18732.5 58 17106.5 59 15455.5 60 13400.5 61 11129.0 62 8869.5 63 6512.5 64 4645.5 65 3521.0 66 2560.5 67 1914.5 68 1490.0 69 1091.0 70 797.0 71 627.5 72 563.0 73 537.5 74 440.5 75 348.5 76 254.5 77 157.5 78 103.0 79 68.0 80 45.5 81 30.5 82 22.5 83 15.5 84 9.0 85 5.5 86 3.0 87 1.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022546177912539385 2 0.0 3 0.0 4 1.8556525030896616E-4 5 0.02273174316284835 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003108217942675183 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011133915018537968 24-25 0.0 26-27 5.103044383496569E-4 28-29 9.742175641220722E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.278262515448307E-4 42-43 0.015123567900180742 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.639131257724154E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.391739377317246E-4 82-83 0.0020412177533986275 84-85 4.175218131951738E-4 86-87 0.0 88-89 0.0 90-91 0.011226697643692453 92-93 0.0025515221917482844 94-95 0.0 96-97 0.0 98-99 0.001994826440821386 100-101 0.019762699157904896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1077788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.51862419514086 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75829847705967 40.83758855770291 2 12.797892274485125 13.442553897071619 3 4.013397106245773 6.323342831063627 4 1.7042730457004496 3.5802430245220016 5 0.9256189787746872 2.4306117647078938 6 0.5643612371594688 1.7783685434809744 7 0.35989844356851364 1.3230959774333615 8 0.27765887553807345 1.1665809711063577 9 0.2090307824167376 0.9880208196264809 >10 1.2211134015965623 12.212723929726714 >50 0.10812584094292367 3.9332324597820842 >100 0.054669926531786685 5.410008068356008 >500 0.0023000290544314623 0.8289280764354312 >1k 0.002830804990069492 2.8452647268836837 >5k 1.7692531187934324E-4 0.7163105639772155 >10k+ 3.538506237586865E-4 2.18312578812358 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12779 1.1856691668491393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10716 0.9942586111554406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7709 0.7152612573159101 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4393 0.40759407230364414 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4051 0.3758624145008109 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2829 0.2624820465620326 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2381 0.2209154304928242 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2310 0.21432786410685592 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2045 0.18974046844091788 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.18807038118813718 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1445 0.13407089334822803 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.1325863713457563 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1257 0.11662775981918522 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1196 0.11096801968476175 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.10122584404354104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.278262515448306E-5 0.0 20-21 0.0 0.0 0.0 1.391739377317246E-4 0.0 22-23 0.0 0.0 0.0 3.2473918804069076E-4 0.0 24-25 0.0 0.0 0.0 6.494783760813815E-4 0.0 26-27 0.0 0.0 0.0 9.742175641220723E-4 0.0 28-29 0.0 0.0 0.0 0.0017628698779351784 0.0 30-31 0.0 0.0 0.0 0.0033865658181386326 0.0 32-33 0.0 0.0 0.0 0.00575252275957795 0.0 34-35 0.0 0.0 0.0 0.007747349200399337 0.0 36-37 0.0 0.0 0.0 0.010484436642456588 0.0 38-39 0.0 0.0 0.0 0.01609778546430281 0.0 40-41 0.0 0.0 0.0 0.02212865609934421 0.0 42-43 0.0 0.0 0.0 0.02751004835830423 0.0 44-45 0.0 0.0 0.0 0.030386309738093205 0.0 46-47 0.0 0.0 0.0 0.035814093309630465 0.0 48-49 0.0 0.0 0.0 0.04268000757106222 0.0 50-51 0.0 0.0 0.0 0.05214383533681949 0.0 52-53 0.0 0.0 0.0 0.06805605555081333 0.0 54-55 0.0 0.0 0.0 0.08188066669883132 0.0 56-57 0.0 0.0 0.0 0.10915875849424933 0.0 58-59 0.0 0.0 0.0 0.15633872338530397 0.0 60-61 0.0 0.0 0.0 0.20542073209202552 0.0 62-63 0.0 0.0 0.0 0.23854412927217597 0.0 64-65 0.0 0.0 0.0 0.2872550074782796 0.0 66-67 0.0 0.0 0.0 0.36013575953712607 0.0 68-69 0.0 0.0 0.0 0.4592739945146912 0.0 70-71 0.0 0.0 0.0 0.5635616651883302 0.0 72-73 0.0 0.0 0.0 0.6763389460636043 0.0 74-75 0.0 0.0 0.0 0.7950543149487654 0.0 76-77 0.0 0.0 0.0 0.9056512041329092 0.0 78-79 0.0 0.0 0.0 1.042923098048967 0.0 80-81 0.0 0.0 0.0 1.2110452148288902 0.0 82-83 0.0 0.0 0.0 1.3748993308517075 0.0 84-85 0.0 0.0 0.0 1.5675624519849916 0.0 86-87 0.0 0.0 0.0 1.8034622764402646 0.0 88-89 0.0 0.0 0.0 2.0711401500109483 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1125 0.0 34.20989 1 TACCTGG 1130 0.0 33.634922 2 TATCACG 85 1.6299166E-5 33.535995 2 ATTGCCG 85 1.6319886E-5 33.529774 8 GTACCGT 70 0.008394701 27.140629 6 ACCTGGG 1495 0.0 24.787476 3 GAGTACT 9385 0.0 23.180857 12-13 CATGGGG 2545 0.0 22.961285 4 GTACATG 10195 0.0 22.883028 1 GGTATCA 12945 0.0 22.79338 1 GTATCAA 16955 0.0 22.614912 1 TACATGG 10280 0.0 22.59928 2 AGTACTT 9545 0.0 22.369282 12-13 ACATGGG 10165 0.0 22.013666 3 GTGTAGC 400 0.0 21.381182 1 GTACTTT 10250 0.0 21.060469 14-15 ATGGGAG 2310 0.0 20.566809 5 GTATAGG 255 1.1876182E-8 20.496122 1 TACTTTT 11030 0.0 19.312786 14-15 CATGGGA 7180 0.0 19.255173 4 >>END_MODULE