FastQCFastQC Report
Thu 26 May 2016
SRR937533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035025
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109781.0606507089200743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85350.8246177628559695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62140.600371971691505No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41160.3976715538272022No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34810.33632037873481313No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23520.2272408879012584No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.19294219946378108No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.18830463032293907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.14328156324726454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14790.1428950991521944No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14620.14125262674814618No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14000.13526243327455859No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13070.1262771430641772No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT12510.12086664573319485No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.10579454602545832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGT350.00839102627.14422844-45
TACCTGG9500.026.0227282
GTACATG102050.024.8783951
TACATGG101850.024.3192272
GTACCTG10600.024.2204421
ATGGGCG2202.1555024E-923.7627035
ATGGGAT26250.023.5363885
ACATGGG103400.023.5363083
GAGTACT75350.023.19620912-13
GTATCAA152600.023.1487961
ATGGGAG22000.022.0345065
GTATAGA3301.8189894E-1221.6108321
CATTCCG1100.002877136521.5909549
AGTACTT78450.021.58335912-13
CATGGGG24450.021.1871974
GTACTTT82850.021.12401214-15
CATGGGA74500.021.1153134
GGTATCA115500.020.5405791
TATCAAC177800.019.8936422
ATCAACG178150.019.7439843
Produced by FastQC (version 0.11.5)