##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937533_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035025 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.707735561942947 33.0 31.0 34.0 30.0 34.0 2 31.81101712519021 33.0 31.0 34.0 30.0 34.0 3 31.803829859182144 33.0 31.0 34.0 30.0 34.0 4 35.42493466341393 37.0 35.0 37.0 33.0 37.0 5 35.427458273954734 37.0 35.0 37.0 33.0 37.0 6 35.50056472065892 37.0 35.0 37.0 33.0 37.0 7 35.44905292142702 37.0 35.0 37.0 33.0 37.0 8 35.445161227989665 37.0 35.0 37.0 33.0 37.0 9 37.04022801381609 39.0 37.0 39.0 33.0 39.0 10-11 36.9199743967537 39.0 37.0 39.0 33.0 39.0 12-13 36.79298471051423 39.0 37.0 39.0 33.0 39.0 14-15 37.85097606338011 40.0 38.0 41.0 33.0 41.0 16-17 37.79352865872805 40.0 37.5 41.0 33.0 41.0 18-19 37.821063742421686 40.0 38.0 41.0 33.0 41.0 20-21 37.783198473466825 40.0 38.0 41.0 33.0 41.0 22-23 37.585944300862295 40.0 37.0 41.0 32.0 41.0 24-25 37.40828144247723 40.0 37.0 41.0 32.0 41.0 26-27 37.31901258423709 39.5 37.0 41.0 32.0 41.0 28-29 37.34943745320162 40.0 37.0 41.0 32.0 41.0 30-31 37.09190550952876 39.0 36.5 41.0 31.5 41.0 32-33 36.791254800608684 39.0 35.5 41.0 31.0 41.0 34-35 36.74257868167436 39.0 35.0 41.0 31.0 41.0 36-37 36.60029709427309 39.0 35.0 41.0 30.5 41.0 38-39 36.43088476123765 39.0 35.0 41.0 30.0 41.0 40-41 36.1527248134103 39.0 35.0 41.0 30.0 41.0 42-43 35.83902369507983 39.0 35.0 41.0 29.0 41.0 44-45 35.48729740827517 39.0 35.0 40.5 27.5 41.0 46-47 34.96319702422647 39.0 35.0 40.0 24.5 41.0 48-49 34.471402623125044 38.0 34.0 40.0 22.5 41.0 50-51 33.48642255017995 37.5 33.5 39.5 19.5 40.5 52-53 33.557513103548224 38.0 33.5 39.5 19.0 40.5 54-55 33.900597570107 38.0 34.0 40.0 18.5 41.0 56-57 33.719212096326174 38.0 34.0 40.0 16.0 41.0 58-59 33.48024540470037 38.0 33.5 40.0 13.5 41.0 60-61 33.15584406173764 37.5 33.0 40.0 9.0 41.0 62-63 32.79637979758943 37.0 33.0 40.0 8.0 41.0 64-65 32.41683437598125 36.5 32.0 39.5 6.5 41.0 66-67 32.014746986787756 36.0 31.5 39.0 2.0 41.0 68-69 31.535783193642665 35.5 31.0 39.0 2.0 40.0 70-71 31.096615540687424 35.0 31.0 38.5 2.0 40.0 72-73 30.637154658100044 35.0 30.0 37.5 2.0 40.0 74-75 30.183159827057317 35.0 30.0 37.0 2.0 39.0 76-77 29.67646578585058 34.5 29.0 36.5 2.0 39.0 78-79 29.160834279365233 34.0 29.0 36.0 2.0 38.5 80-81 28.637458032414678 34.0 28.5 35.5 2.0 37.5 82-83 28.10987947151035 34.0 27.0 35.0 2.0 37.0 84-85 27.641886427864062 34.0 26.0 35.0 2.0 36.5 86-87 27.15618849786237 33.0 26.0 35.0 2.0 36.0 88-89 26.64188256322311 33.0 25.0 35.0 2.0 36.0 90-91 26.11946329798797 32.5 23.5 35.0 2.0 35.0 92-93 25.572466365546724 32.0 21.0 35.0 2.0 35.0 94-95 24.94572015168716 32.0 18.5 34.5 2.0 35.0 96-97 24.202736648873216 31.5 5.0 34.0 2.0 35.0 98-99 23.27053259583102 31.0 2.0 34.0 2.0 35.0 100-101 21.00891476051303 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17282376404838473 1101 2 0.27176069647130774 1101 3 0.11983877240665919 1101 4 0.027407117151604155 1101 5 -0.06705918407360656 1101 6 0.08889881561345447 1101 7 0.15780381476902505 1101 8 0.13256345725319818 1101 9 0.1584516920611705 1101 10-11 0.1492006176000018 1101 12-13 0.2457842827511385 1101 14-15 0.3028824094037432 1101 16-17 0.33263182949905 1101 18-19 0.3168382032579089 1101 20-21 0.39552246061494856 1101 22-23 0.3672818132834408 1101 24-25 0.324118678681792 1101 26-27 0.3870988059005569 1101 28-29 0.13710926529693523 1101 30-31 0.09137981880086699 1101 32-33 0.2949114287101722 1101 34-35 0.4891082313335957 1101 36-37 0.30474031929218626 1101 38-39 0.4066508703954028 1101 40-41 0.4123783278282147 1101 42-43 0.5773724035562111 1101 44-45 0.49030778145761644 1101 46-47 0.40931576834029926 1101 48-49 0.33229676095813687 1101 50-51 0.329242896284903 1101 52-53 0.5284868725698928 1101 54-55 0.6119478493733865 1101 56-57 0.5452023707152165 1101 58-59 0.6504296399300813 1101 60-61 0.7061915817330373 1101 62-63 0.7701165248098292 1101 64-65 0.6891670581032372 1101 66-67 0.7474340933055998 1101 68-69 0.8378531334877124 1101 70-71 0.690328086321145 1101 72-73 0.7759842821213105 1101 74-75 0.7772536161917216 1101 76-77 0.7410026727499961 1101 78-79 0.6619764180179146 1101 80-81 0.7544490492006943 1101 82-83 0.8947206805999599 1101 84-85 1.0003654847254815 1101 86-87 1.0652556940562015 1101 88-89 1.1018843239412028 1101 90-91 1.251102901377756 1101 92-93 1.2709525870515073 1101 94-95 1.0841710812387113 1101 96-97 1.1593272032237678 1101 98-99 1.3201914629823932 1101 100-101 1.7105037811102584 1102 1 0.24937760255810915 1102 2 0.13752512918869542 1102 3 0.2324796938510154 1102 4 0.22387403106922932 1102 5 0.1696588119650997 1102 6 0.061086495199141666 1102 7 -0.011342186604188953 1102 8 0.16892627460372012 1102 9 0.29211463267316873 1102 10-11 0.20334489222056362 1102 12-13 0.27629013255232593 1102 14-15 0.4545465758053666 1102 16-17 0.42011096172734597 1102 18-19 0.5069707332303821 1102 20-21 0.5694160080201058 1102 22-23 0.5452322056589693 1102 24-25 0.5029478038802324 1102 26-27 0.4828349553649929 1102 28-29 0.3904590289433969 1102 30-31 0.4787713166257035 1102 32-33 0.5224486289684407 1102 34-35 0.6693150048450818 1102 36-37 0.7165742398124166 1102 38-39 0.6378818198090528 1102 40-41 0.6800744712319542 1102 42-43 0.7175598074074827 1102 44-45 0.4511661208539266 1102 46-47 0.5635403744973715 1102 48-49 0.500414218359218 1102 50-51 0.4330350635164635 1102 52-53 0.31744698526610193 1102 54-55 0.2835058443746803 1102 56-57 0.4096307882863286 1102 58-59 0.5108309017562931 1102 60-61 0.610573165404098 1102 62-63 0.67054227512822 1102 64-65 0.2302525098367454 1102 66-67 0.6191019043155919 1102 68-69 0.6156702314785392 1102 70-71 0.7910980951528366 1102 72-73 0.7111976145628311 1102 74-75 0.7561780199160495 1102 76-77 0.8833792304105685 1102 78-79 0.8261052815416612 1102 80-81 0.698080130202456 1102 82-83 0.7885967147746484 1102 84-85 0.8232972377275054 1102 86-87 1.0408337466887154 1102 88-89 1.1199203577882848 1102 90-91 1.0741929969156345 1102 92-93 1.2664415310569446 1102 94-95 1.7035982088641575 1102 96-97 1.9321438848829224 1102 98-99 1.9725982012763055 1102 100-101 2.417200168852908 1103 1 0.045440236882555496 1103 2 0.21058111515488775 1103 3 0.21395002061524693 1103 4 0.12486659542277323 1103 5 -0.014149205957593836 1103 6 0.09891859938537095 1103 7 0.15229949687161337 1103 8 0.02856705454741615 1103 9 0.029822170957700678 1103 10-11 0.054298422402368374 1103 12-13 0.17797454622736808 1103 14-15 0.19502349280590892 1103 16-17 -0.009703153716046131 1103 18-19 0.04141195014545218 1103 20-21 -0.0275776342313776 1103 22-23 0.0014789280434541752 1103 24-25 0.09255632614291898 1103 26-27 0.0427847723469057 1103 28-29 -0.027215525135773078 1103 30-31 0.00872888760383006 1103 32-33 -0.05831809198941329 1103 34-35 0.20383283705493938 1103 36-37 0.22820391318028754 1103 38-39 0.31811764961214095 1103 40-41 0.3988326033510674 1103 42-43 0.39840129432542426 1103 44-45 0.14801756247636888 1103 46-47 0.07882479556974431 1103 48-49 0.33398400234371906 1103 50-51 0.36104838929394134 1103 52-53 0.3562361201605384 1103 54-55 0.4450795753678065 1103 56-57 0.4948728992226279 1103 58-59 0.2573676922887529 1103 60-61 0.6201183743723107 1103 62-63 0.7256841521236836 1103 64-65 0.8097326222234571 1103 66-67 0.6972927662244501 1103 68-69 0.8364038493452313 1103 70-71 0.8472617295710698 1103 72-73 0.8248570648613018 1103 74-75 0.6005271135438974 1103 76-77 0.6234241956628885 1103 78-79 0.8891273057970075 1103 80-81 0.6365484715737892 1103 82-83 0.911710558497937 1103 84-85 1.0310320140702878 1103 86-87 0.9289169641083923 1103 88-89 0.8950590909147564 1103 90-91 1.3164832526389958 1103 92-93 1.2061211866579846 1103 94-95 1.2545784223038012 1103 96-97 1.5560103417821978 1103 98-99 1.620070094691222 1103 100-101 2.2632047472772463 1104 1 0.09838242874901226 1104 2 0.13010879690457244 1104 3 0.22254822819454745 1104 4 0.16351420887199453 1104 5 0.17238477865613788 1104 6 0.30625996642106657 1104 7 0.17832738622858813 1104 8 0.16843838468033567 1104 9 0.2766089232798663 1104 10-11 0.3843986803952504 1104 12-13 0.35696935371242233 1104 14-15 0.48464032905740595 1104 16-17 0.536516294188516 1104 18-19 0.47362933597310786 1104 20-21 0.5940639096204166 1104 22-23 0.4674313323224908 1104 24-25 0.4091131242320003 1104 26-27 0.5082123267330161 1104 28-29 0.532295454723986 1104 30-31 0.6490830796382454 1104 32-33 0.5715461001849604 1104 34-35 0.44225698108308364 1104 36-37 0.5183665933204367 1104 38-39 0.5037955457338512 1104 40-41 0.3909621876232947 1104 42-43 0.3347848219698477 1104 44-45 0.5434627984722056 1104 46-47 0.6560593731472224 1104 48-49 0.7361589803000257 1104 50-51 0.8779812131729798 1104 52-53 0.7875904194975973 1104 54-55 0.7053211462785285 1104 56-57 0.8251714338417244 1104 58-59 0.7616178100883957 1104 60-61 0.8052061059477467 1104 62-63 0.8814992556707395 1104 64-65 0.6206816907898514 1104 66-67 0.5215566934079376 1104 68-69 0.6471523086192121 1104 70-71 0.6467130864829898 1104 72-73 0.7897877613487729 1104 74-75 0.9270560595986623 1104 76-77 0.878736842912442 1104 78-79 0.7684024997582029 1104 80-81 0.7673527282230559 1104 82-83 0.8663681930811151 1104 84-85 0.8599846002972917 1104 86-87 1.1779500739723012 1104 88-89 1.0941824749602524 1104 90-91 1.0638625021736274 1104 92-93 1.4593111076739085 1104 94-95 1.4305710884012974 1104 96-97 1.8315378742412456 1104 98-99 2.069873450931336 1104 100-101 2.158677975267377 1105 1 0.1422730483881658 1105 2 0.11848003447084565 1105 3 0.14001378641708584 1105 4 0.10912036834136529 1105 5 0.018142958427880274 1105 6 0.13708426574223864 1105 7 0.2192601074470062 1105 8 0.3339851249071373 1105 9 0.09823637246719841 1105 10-11 0.17915133284355989 1105 12-13 0.36487186545084427 1105 14-15 0.419258879109627 1105 16-17 0.24630156121413904 1105 18-19 0.226756890701445 1105 20-21 0.34742832999402395 1105 22-23 0.35039048210890655 1105 24-25 0.3288270152848085 1105 26-27 0.2536223243196716 1105 28-29 0.27998610062807217 1105 30-31 0.32554282863628714 1105 32-33 0.1524613297864974 1105 34-35 0.2294306204463723 1105 36-37 0.176333632898654 1105 38-39 0.18470279959812785 1105 40-41 0.18174622322258216 1105 42-43 0.5222414624930565 1105 44-45 0.33056183718959886 1105 46-47 0.3646039317348553 1105 48-49 0.3201648080887267 1105 50-51 0.26714493649465254 1105 52-53 0.4834236468315467 1105 54-55 0.3983862427333875 1105 56-57 0.3649418288676145 1105 58-59 0.6040306493752325 1105 60-61 0.5049574142093078 1105 62-63 0.7379744886000807 1105 64-65 0.6944493534381593 1105 66-67 0.6920583604057882 1105 68-69 0.6257467361916014 1105 70-71 0.9937810748301672 1105 72-73 0.9714069872759694 1105 74-75 0.8256730942608037 1105 76-77 0.9697420677079087 1105 78-79 0.9262536848771248 1105 80-81 0.9524266172406719 1105 82-83 0.9763422650593938 1105 84-85 1.095910689965205 1105 86-87 1.2580773557379104 1105 88-89 1.0940676518462915 1105 90-91 1.2901331368154203 1105 92-93 1.4332620747664286 1105 94-95 1.548851959489319 1105 96-97 1.661345954625677 1105 98-99 1.9030203399468881 1105 100-101 2.216157257258505 1106 1 0.004175287143027617 1106 2 0.14014307861515718 1106 3 0.17346282419584824 1106 4 0.09058675097344349 1106 5 0.14661093785339574 1106 6 -0.047166953479838014 1106 7 0.09564090122996305 1106 8 0.022638519805326496 1106 9 0.17144664817539734 1106 10-11 0.04023282698084074 1106 12-13 -0.0048928688434415335 1106 14-15 0.04842432925758544 1106 16-17 0.08632736943312125 1106 18-19 0.37755546764490333 1106 20-21 0.31175026950330675 1106 22-23 0.2766348455722465 1106 24-25 0.265327622643035 1106 26-27 0.2388006209051241 1106 28-29 0.30499520199020935 1106 30-31 0.24587761458306545 1106 32-33 0.24626771768114253 1106 34-35 0.26201025285947566 1106 36-37 0.23483682828777575 1106 38-39 0.22163955143913228 1106 40-41 0.2597908541398297 1106 42-43 0.2692942431844756 1106 44-45 0.37325785890310925 1106 46-47 0.40314793006236727 1106 48-49 0.5574486732660162 1106 50-51 0.39699379981562544 1106 52-53 0.3260284746986528 1106 54-55 0.2186359624835248 1106 56-57 0.29182058430590274 1106 58-59 0.24303061693473182 1106 60-61 0.29018220884292845 1106 62-63 0.5348410968738477 1106 64-65 0.43802523356691836 1106 66-67 0.5869471796076766 1106 68-69 0.3363598255723872 1106 70-71 0.49220266434251414 1106 72-73 0.5193100694837973 1106 74-75 0.8336696771949406 1106 76-77 0.9806110765785867 1106 78-79 0.8842846907849022 1106 80-81 0.6393293065580146 1106 82-83 0.30112284113055665 1106 84-85 0.44842140434103683 1106 86-87 0.7126721919314143 1106 88-89 0.6612194841937935 1106 90-91 0.9565081994436042 1106 92-93 0.8072804793427046 1106 94-95 1.014215959907439 1106 96-97 1.2469120953958175 1106 98-99 1.6833909546222507 1106 100-101 1.8642906670072357 1107 1 0.03786986332907105 1107 2 0.19344671468329722 1107 3 0.09073673344927968 1107 4 0.09024176035541132 1107 5 0.1557011082063653 1107 6 0.1872309012251847 1107 7 0.20309255083178357 1107 8 0.061530856137935075 1107 9 0.020483333901502476 1107 10-11 0.148683619646647 1107 12-13 0.0599254754970886 1107 14-15 0.21766919133521867 1107 16-17 0.16323405205652364 1107 18-19 0.13811240217794563 1107 20-21 0.07984343913231129 1107 22-23 0.12804768188269122 1107 24-25 0.2798877780734941 1107 26-27 0.3539255414908027 1107 28-29 0.2315772092889361 1107 30-31 0.2672740509112401 1107 32-33 0.222483680330555 1107 34-35 0.27326603348385703 1107 36-37 0.13850466216038626 1107 38-39 0.26122002216294504 1107 40-41 0.29578586027135145 1107 42-43 0.3458156739721119 1107 44-45 0.4668784874213472 1107 46-47 0.5892947468405865 1107 48-49 0.7919396089514947 1107 50-51 0.494622820396863 1107 52-53 0.5158013866558662 1107 54-55 0.6177764408902107 1107 56-57 0.6716138854302685 1107 58-59 0.545088371466889 1107 60-61 0.7277019700828262 1107 62-63 0.7431411869998783 1107 64-65 0.7425847241332306 1107 66-67 0.5646989784345564 1107 68-69 0.6838318643413572 1107 70-71 1.091748709878317 1107 72-73 1.0245030278337488 1107 74-75 1.0690205038426939 1107 76-77 1.2244359661372606 1107 78-79 1.2256628802859595 1107 80-81 1.2107977698353523 1107 82-83 1.0650521143601708 1107 84-85 1.1192885349351265 1107 86-87 1.143059438359618 1107 88-89 1.274866928899261 1107 90-91 1.2544589403977575 1107 92-93 1.3303767516710572 1107 94-95 1.408314921241896 1107 96-97 1.5930038023237003 1107 98-99 1.832396243985901 1107 100-101 2.325979348064518 1108 1 -0.017031288803085687 1108 2 -0.06533490483037596 1108 3 0.04022930565063021 1108 4 0.006974490759986907 1108 5 -0.011199939001031112 1108 6 0.004193300271531086 1108 7 -0.03718004952437326 1108 8 -0.068720846703485 1108 9 0.06405406922004886 1108 10-11 0.08165892152392473 1108 12-13 0.20706642151038324 1108 14-15 -9.211445114516437E-4 1108 16-17 -0.031537998901931985 1108 18-19 0.12224143923096165 1108 20-21 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-0.8377607085014844 2316 88-89 -0.5086939357196236 2316 90-91 -0.4392710213356139 2316 92-93 -0.6765939795317557 2316 94-95 -0.6363251656336857 2316 96-97 -0.267048943565225 2316 98-99 0.013477534708830063 2316 100-101 -0.13018985247328274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4816.0 3 1012.0 4 878.0 5 937.0 6 1048.0 7 1283.0 8 1547.0 9 1892.0 10 2405.0 11 3365.0 12 4859.0 13 6794.0 14 7161.0 15 9417.0 16 12577.0 17 17384.0 18 14697.0 19 8645.0 20 7698.0 21 8018.0 22 8627.0 23 9164.0 24 10304.0 25 11721.0 26 13324.0 27 15558.0 28 18409.0 29 21807.0 30 26438.0 31 32766.0 32 41294.0 33 54057.0 34 72134.0 35 101223.0 36 138420.0 37 183464.0 38 142221.0 39 17658.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.03931609684429 19.000098852343672 14.723378191622361 20.237206859189683 2 14.804848743241411 21.07740160074417 41.737466328171934 22.380283327842484 3 19.509456373569627 27.80680533366403 30.2204791694319 22.463259123334442 4 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20.582556789389088 24-25 20.65295359568802 26.166012764404485 32.91858215016229 20.262451489745207 26-27 20.767823594591313 26.114938277760487 32.87144318363897 20.245794944009234 28-29 20.14754148363579 26.20698703867868 33.45822926011873 20.1872422175668 30-31 20.175050869711086 26.76882082484698 33.10701980227259 19.94910850316934 32-33 19.98088546578847 26.717161055927924 32.64489378554926 20.657059692734347 34-35 20.509432957499648 26.391560928142987 32.73064391977266 20.3683621945847 36-37 20.87136246587543 26.239859435807084 32.62067029371333 20.268107804604156 38-39 20.836407006619773 26.268579991984247 32.71709814263006 20.177914858765924 40-41 20.506890695129396 26.511594363535778 32.8884096053962 20.09310533593863 42-43 20.21395142598785 26.98035043150437 32.31359727678822 20.49210086571956 44-45 20.361446657937474 27.325938529381155 31.540783158628464 20.771831654052903 46-47 20.526999847520024 27.272472039286587 30.86848238856255 21.33204572463084 48-49 20.812187655975343 27.491599419257362 30.60033471612306 21.095878208644237 50-51 21.289975864998922 27.78991655632025 30.234644498938497 20.685463079742334 52-53 21.01732470592451 28.061530088354598 30.188441706158383 20.732703499562515 54-55 20.40826798831584 28.01144598785304 30.402400733503672 21.177885290327453 56-57 20.875231206571637 28.57021178570357 29.57121162406465 20.983345383660144 58-59 20.859124024712607 27.80501898549785 30.0192516723015 21.31660531748805 60-61 21.047460624519346 27.714588686364365 30.26482953948728 20.973121149629005 62-63 21.477457645659147 27.593655827479086 30.239852237778685 20.68903428908308 64-65 21.101440914658728 27.5336514286461 30.507579647643055 20.857328009052118 66-67 20.723908054210344 27.952921680361868 30.244548619816143 21.078621645611648 68-69 20.73971893294225 28.33709654389646 30.067882994402375 20.855301528758915 70-71 20.71276979331273 28.318067530908714 29.67662311577246 21.292539560006098 72-73 20.82782793538139 28.258300204147417 29.359476592741878 21.55439526772932 74-75 20.694548640185083 28.376111398825802 29.59135371644023 21.337986244548883 76-77 20.99577734715938 27.92934136200523 29.87438539658228 21.200495894253116 78-79 20.81708332526481 28.065539009701595 29.88391007145485 21.233467593578744 80-81 20.592892205861638 28.78168212663605 29.491454702200958 21.13397096530135 82-83 20.693953201094146 28.7600627909442 29.091443305905457 21.454540702056196 84-85 20.60950063212349 28.24522325672345 29.171579418136616 21.97369669301644 86-87 20.929717024967474 28.114642060591045 29.499653444644075 21.45598746979741 88-89 21.38092340697219 27.637007337746923 29.467837879309332 21.514231375971555 90-91 20.678613748664013 28.067446057095058 29.7803172292012 21.47362296503973 92-93 20.716122311866993 28.29720436103415 29.590906186650866 21.39576714044799 94-95 20.42560750766842 28.367509043094646 29.296770817753266 21.910112631483667 96-97 20.602247539031872 27.99676710578533 29.036713675916875 22.364271679265922 98-99 20.64991217468453 28.037838698607427 28.975045413117794 22.33720371359025 100-101 21.162106269821717 27.654071592188867 29.585462612844353 21.598359525145057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1957.0 1 1281.0 2 1162.5 3 1499.0 4 1010.5 5 826.0 6 1264.0 7 2671.5 8 4168.5 9 4527.0 10 3800.0 11 3046.5 12 2988.0 13 3114.0 14 3173.5 15 3246.0 16 3284.0 17 3292.0 18 3433.0 19 3562.5 20 3782.5 21 4145.5 22 4662.0 23 5294.0 24 5992.5 25 6876.5 26 8270.0 27 9894.0 28 10863.5 29 12194.5 30 14103.0 31 15516.5 32 17253.0 33 19924.5 34 22988.5 35 25069.0 36 27636.0 37 30546.0 38 31510.0 39 31786.0 40 31873.0 41 32352.5 42 33282.0 43 34483.0 44 35897.0 45 36326.0 46 35499.0 47 36664.5 48 47146.0 49 47332.0 50 37409.0 51 33275.0 52 29021.5 53 26761.5 54 25206.5 55 22995.5 56 20382.5 57 18095.0 58 16554.5 59 14955.0 60 13009.0 61 11053.5 62 8880.5 63 6435.0 64 4627.5 65 3607.0 66 2634.0 67 1929.5 68 1444.5 69 1102.5 70 876.5 71 673.5 72 563.5 73 536.0 74 433.0 75 311.5 76 233.5 77 162.5 78 110.0 79 78.5 80 56.0 81 34.5 82 20.5 83 13.5 84 11.5 85 11.0 86 9.0 87 5.0 88 2.0 89 2.0 90 2.5 91 2.5 92 3.5 93 2.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3075288036520857 2 0.29033115142146326 3 0.27670829207023984 4 0.2625057365764112 5 0.25980048791092003 6 0.2149706528827806 7 0.2071447549576097 8 0.21912514190478488 9 0.2195116059998551 10-11 0.22168546653462476 12-13 0.21714451341755028 14-15 0.21410110866887272 16-17 0.21265186831235958 18-19 0.2141494166807565 20-21 0.19985024516316033 22-23 0.19086495495277891 24-25 0.19284558344001354 26-27 0.20593705466051543 28-29 0.22207193062969496 30-31 0.21637158522740996 32-33 0.22250670273664888 34-35 0.21352141252626747 36-37 0.19757976860462306 38-39 0.1989323929373687 40-41 0.193376971570735 42-43 0.2055022825535615 44-45 0.2021690297335813 46-47 0.20313518997125674 48-49 0.2134731045143837 50-51 0.22149223448708968 52-53 0.23424554962440522 54-55 0.21004323567063599 56-57 0.17951257216009275 58-59 0.18047873239776815 60-61 0.18613076978816936 62-63 0.1945846718678293 64-65 0.1832322890751431 66-67 0.1872418540614961 68-69 0.19299050747566485 70-71 0.18767662616845004 72-73 0.18820801429917153 74-75 0.19129972705973286 76-77 0.20709644694572593 78-79 0.21303833240742978 80-81 0.20178256563851116 82-83 0.20033332528199801 84-85 0.19313543151131615 86-87 0.19332866355885123 88-89 0.20081640540083576 90-91 0.2010579454602546 92-93 0.1989323929373687 94-95 0.19849762083041472 96-97 0.1830873650394918 98-99 0.1940532837371078 100-101 0.18448829738412115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.99588416271912 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2800205206505 43.600748249716894 2 12.038154273968624 13.24097875968241 3 3.664404279943028 6.045814599153568 4 1.578961070782075 3.4734544058469576 5 0.8445140388203053 2.322239812637579 6 0.5142481304426493 1.696891836763129 7 0.34424921309344897 1.325260289247617 8 0.24935582984004823 1.0970835466545594 9 0.19073203532955849 0.9440529228993643 >10 1.1392618792536002 11.815899527628222 >50 0.10055760253548712 3.7865350901576447 >100 0.04991670807600401 4.785274257838062 >500 0.002812208632303282 1.0210453285242744 >1k 0.0022849195137464163 2.3578868407358353 >5k 3.5152607903791023E-4 1.4256742923794872 >10k+ 1.7576303951895512E-4 1.0611602401343827 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10978 1.0606507089200743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8535 0.8246177628559695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6214 0.600371971691505 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4116 0.3976715538272022 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3481 0.33632037873481313 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2352 0.2272408879012584 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.19294219946378108 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 0.18830463032293907 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1483 0.14328156324726454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1479 0.1428950991521944 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1462 0.14125262674814618 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1400 0.13526243327455859 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1307 0.1262771430641772 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1251 0.12086664573319485 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1095 0.10579454602545832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.8308011883770923E-5 0.0 18-19 0.0 0.0 0.0 9.661602376754185E-5 0.0 20-21 0.0 0.0 0.0 9.661602376754185E-5 0.0 22-23 0.0 0.0 0.0 5.796961426052511E-4 0.0 24-25 0.0 0.0 0.0 6.763121663727929E-4 0.0 26-27 0.0 0.0 0.0 9.178522257916476E-4 0.0 28-29 0.0 0.0 0.0 0.001884012463467066 0.0 30-31 0.0 0.0 0.0 0.003816332938817903 0.0 32-33 0.0 0.0 0.0 0.005652037390401198 0.0 34-35 0.0 0.0 0.0 0.007777589913287119 0.0 36-37 0.0 0.0 0.0 0.011159150745151083 0.0 38-39 0.0 0.0 0.0 0.017390884278157534 0.0 40-41 0.0 0.0 0.0 0.025216782203328424 0.0 42-43 0.0 0.0 0.0 0.031158667665032246 0.0 44-45 0.0 0.0 0.0 0.0337189922948721 0.0 46-47 0.0 0.0 0.0 0.03908118161397067 0.0 48-49 0.0 0.0 0.0 0.04748677568174682 0.0 50-51 0.0 0.0 0.0 0.0578246902248738 0.0 52-53 0.0 0.0 0.0 0.07647158281200937 0.0 54-55 0.0 0.0 0.0 0.09149537450786213 0.0 56-57 0.0 0.0 0.0 0.12357189439868603 0.0 58-59 0.0 0.0 0.0 0.17183159827057318 0.0 60-61 0.0 0.0 0.0 0.21753097751262046 0.0 62-63 0.0 0.0 0.0 0.2512016617956088 0.0 64-65 0.0 0.0 0.0 0.3022632303567546 0.0 66-67 0.0 0.0 0.0 0.3761261805270404 0.0 68-69 0.0 0.0 0.0 0.4793604019226589 0.0 70-71 0.0 0.0 0.0 0.5891162049225864 0.0 72-73 0.0 0.0 0.0 0.7066979058476848 0.0 74-75 0.0 0.0 0.0 0.8396415545518224 0.0 76-77 0.0 0.0 0.0 0.950653365860728 0.0 78-79 0.0 0.0 0.0 1.0871718074442644 0.0 80-81 0.0 0.0 0.0 1.2569261612038356 0.0 82-83 0.0 0.0 0.0 1.4282263713436874 0.0 84-85 0.0 0.0 0.0 1.63348711383783 0.0 86-87 0.0 0.0 0.0 1.878746890171735 0.0 88-89 0.0 0.0 0.0 2.163619236250332 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCGT 35 0.008391026 27.144228 44-45 TACCTGG 950 0.0 26.022728 2 GTACATG 10205 0.0 24.878395 1 TACATGG 10185 0.0 24.319227 2 GTACCTG 1060 0.0 24.220442 1 ATGGGCG 220 2.1555024E-9 23.762703 5 ATGGGAT 2625 0.0 23.536388 5 ACATGGG 10340 0.0 23.536308 3 GAGTACT 7535 0.0 23.196209 12-13 GTATCAA 15260 0.0 23.148796 1 ATGGGAG 2200 0.0 22.034506 5 GTATAGA 330 1.8189894E-12 21.610832 1 CATTCCG 110 0.0028771365 21.590954 9 AGTACTT 7845 0.0 21.583359 12-13 CATGGGG 2445 0.0 21.187197 4 GTACTTT 8285 0.0 21.124012 14-15 CATGGGA 7450 0.0 21.115313 4 GGTATCA 11550 0.0 20.540579 1 TATCAAC 17780 0.0 19.893642 2 ATCAACG 17815 0.0 19.743984 3 >>END_MODULE