FastQCFastQC Report
Thu 26 May 2016
SRR937533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035025
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125691.2143668027342336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104681.011376536798628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74500.7197893770681868No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43500.42027970338880705No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38710.37400062800415446No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.25641892707905606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.2302359846380522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23170.2238593270693945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.19294219946378108No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19590.18927079056061447No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14330.13845076205888746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.1260839110166421No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11760.1136204439506292No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11140.10763025047704161No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10600.10241298519359436No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG10450.10096374483708123No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.10077051278954614No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10410.10057728074201107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1001.2732926E-1147.498842
TACGGGC500.001614785238.004587
ACCGTAT550.002577983534.5496188
TACCTGG9950.031.9841422
ATCACGC1551.3569661E-930.6444133
GTACCTG10550.030.178261
GGACCGA2051.8189894E-1230.119766
TCACGCA1703.6416168E-927.9404954
GATATCG700.00838625927.146139
ACCTGGG13300.023.9279863
CCCGCGA500.001654326723.7494220-21
AGTACTT92600.022.82942412-13
GAGTACT91000.022.60437412-13
GTATCAA163300.022.2320271
GGTATCA123750.022.0413681
GTACATG104000.021.7950041
AGGACCG2858.367351E-1121.6661385
CATGGGG24450.021.563894
TACATGG104450.021.373342
GTACTTT98200.020.91879514-15
Produced by FastQC (version 0.11.5)