##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937533_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035025 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20612255742615 33.0 31.0 34.0 31.0 34.0 2 32.40580758918867 34.0 31.0 34.0 31.0 34.0 3 32.44903553054274 34.0 31.0 34.0 31.0 34.0 4 36.06923021183063 37.0 35.0 37.0 35.0 37.0 5 36.011943672858145 37.0 35.0 37.0 35.0 37.0 6 36.08009564986353 37.0 35.0 37.0 35.0 37.0 7 36.03627351996329 37.0 35.0 37.0 35.0 37.0 8 36.0211308905582 37.0 35.0 37.0 35.0 37.0 9 37.67931402623125 39.0 37.0 39.0 35.0 39.0 10-11 37.60481775802517 39.0 37.0 39.0 35.0 39.0 12-13 37.488639404845294 39.0 37.0 39.0 35.0 39.0 14-15 38.60353662955001 40.0 38.0 41.0 34.0 41.0 16-17 38.578137726141875 40.0 38.0 41.0 34.0 41.0 18-19 38.65261563730345 40.0 38.0 41.0 34.0 41.0 20-21 38.599569092534 40.0 38.0 41.0 34.0 41.0 22-23 38.5332760078259 40.0 38.0 41.0 34.0 41.0 24-25 38.522230863988796 40.0 38.0 41.0 34.0 41.0 26-27 38.3049853868264 40.0 38.0 41.0 34.0 41.0 28-29 38.2290292505012 40.0 38.0 41.0 34.0 41.0 30-31 38.1286316755634 40.0 37.0 41.0 34.0 41.0 32-33 37.89627303688317 40.0 36.5 41.0 33.5 41.0 34-35 37.69503490253858 40.0 36.0 41.0 33.0 41.0 36-37 37.45760827033163 40.0 36.0 41.0 33.0 41.0 38-39 37.27289195913143 40.0 35.5 41.0 33.0 41.0 40-41 37.030315692857656 40.0 35.0 41.0 32.0 41.0 42-43 36.73001473394362 39.0 35.0 41.0 31.0 41.0 44-45 36.2757841598029 39.0 35.0 41.0 30.5 41.0 46-47 35.69968937948359 39.0 35.0 41.0 28.0 41.0 48-49 35.16418830463033 39.0 35.0 41.0 25.5 41.0 50-51 34.83874061013019 39.0 35.0 40.0 24.0 41.0 52-53 34.51614550373179 38.5 35.0 40.0 23.0 41.0 54-55 34.28169319581653 38.0 34.0 40.0 22.0 41.0 56-57 34.025010023912465 38.0 34.0 40.0 20.5 41.0 58-59 33.776366271346106 38.0 34.0 40.0 19.5 41.0 60-61 33.42603318760416 37.5 33.5 40.0 17.5 41.0 62-63 33.00124731286684 37.0 33.0 40.0 13.5 41.0 64-65 32.59125335136832 36.0 32.0 39.0 9.5 41.0 66-67 32.16955870631144 36.0 32.0 39.0 7.5 40.0 68-69 31.71469771261564 35.0 31.0 39.0 6.0 40.0 70-71 31.28822975290452 35.0 31.0 38.0 2.0 40.0 72-73 30.800307238955583 35.0 30.5 37.5 2.0 39.5 74-75 30.31808458732881 34.5 30.0 37.0 2.0 39.0 76-77 29.082937127122534 33.5 28.5 35.5 2.0 38.0 78-79 29.64932248013333 34.0 29.5 36.0 2.0 38.0 80-81 29.479274896741625 34.0 29.5 35.5 2.0 37.0 82-83 29.082985918214536 34.0 29.0 35.0 2.0 37.0 84-85 28.669843240501436 34.0 29.0 35.0 2.0 36.5 86-87 28.310871234994323 34.0 29.0 35.0 2.0 36.0 88-89 27.868516702495107 34.0 27.5 35.0 2.0 36.0 90-91 27.33222337624695 33.0 26.5 35.0 2.0 35.0 92-93 26.879798072510326 33.0 25.5 35.0 2.0 35.0 94-95 26.385530784280572 33.0 25.0 35.0 2.0 35.0 96-97 25.838584092171686 32.5 24.0 34.5 2.0 35.0 98-99 25.022014444095554 32.0 19.0 34.0 2.0 35.0 100-101 22.671000700466173 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2892434729421254 1101 2 0.19917269155877904 1101 3 0.22386344901257615 1101 4 0.15989127013345694 1101 5 0.09464168086811497 1101 6 0.018232285712798557 1101 7 0.06870952409317965 1101 8 0.1558777187613174 1101 9 0.21546026165042775 1101 10-11 0.15321900614046058 1101 12-13 0.22450004421195757 1101 14-15 0.3178388915799175 1101 16-17 0.3355473847375521 1101 18-19 0.1919998712237856 1101 20-21 0.22027755635946988 1101 22-23 0.23329328819561113 1101 24-25 0.05314743025577684 1101 26-27 0.14909357149551283 1101 28-29 0.21540526925828374 1101 30-31 0.22735092284777636 1101 32-33 0.19876189723909476 1101 34-35 0.2328279283615018 1101 36-37 0.05416629662089889 1101 38-39 0.18541134565165152 1101 40-41 0.17267168780030318 1101 42-43 0.28441727640537096 1101 44-45 0.1269712738472748 1101 46-47 0.1538902649280658 1101 48-49 0.27258806442260664 1101 50-51 0.35652198035253235 1101 52-53 0.24626956043710635 1101 54-55 0.34853619125535573 1101 56-57 0.3328009323738357 1101 58-59 0.46351154379193105 1101 60-61 0.47398220595582785 1101 62-63 0.33964325806302753 1101 64-65 0.5454291894195578 1101 66-67 0.4452066837550177 1101 68-69 0.4386110052014125 1101 70-71 0.2513541378444657 1101 72-73 0.4946867721972623 1101 74-75 0.433614174019457 1101 76-77 0.2911743999459695 1101 78-79 0.3897605818174199 1101 80-81 0.6315959327372198 1101 82-83 0.48066994158523 1101 84-85 0.5314356236935467 1101 86-87 0.6472914436362167 1101 88-89 0.8103036981783696 1101 90-91 0.6505395829132219 1101 92-93 0.8289827931342337 1101 94-95 0.978981513311254 1101 96-97 1.0569337958212834 1101 98-99 1.0103819889476853 1101 100-101 1.3075444807524832 1102 1 0.25166988120105316 1102 2 0.1483529276802642 1102 3 0.21907836487945787 1102 4 0.04621634462952784 1102 5 -0.02646715368986463 1102 6 0.12545338785874804 1102 7 0.08078450747403565 1102 8 0.029123153786962064 1102 9 0.12435256006058637 1102 10-11 0.09911841221945394 1102 12-13 0.12261139996531512 1102 14-15 0.2411785813650411 1102 16-17 0.27569424743956716 1102 18-19 0.27538291410321847 1102 20-21 0.21690868240695238 1102 22-23 0.1788080993653125 1102 24-25 0.10130744903847955 1102 26-27 0.08187853004662315 1102 28-29 0.14163471032278352 1102 30-31 0.08181433957939532 1102 32-33 0.1881904854379286 1102 34-35 0.2855041219707388 1102 36-37 0.31016932150934196 1102 38-39 0.09865738128691959 1102 40-41 0.26832252339843876 1102 42-43 0.20902097643362794 1102 44-45 0.4032861652802566 1102 46-47 0.3825887575344282 1102 48-49 0.3169857417014086 1102 50-51 0.5982820258896666 1102 52-53 0.5048084546874989 1102 54-55 0.46105187194397956 1102 56-57 0.494694572600487 1102 58-59 0.4452014818062793 1102 60-61 0.608621819709235 1102 62-63 0.5094915631836301 1102 64-65 0.867736786175719 1102 66-67 0.7630841020618746 1102 68-69 0.6065622711909349 1102 70-71 0.502967252428494 1102 72-73 0.6762339971760696 1102 74-75 0.38937859308662937 1102 76-77 0.16547294933723933 1102 78-79 0.33194166834752536 1102 80-81 0.48792704062991277 1102 82-83 0.3737051222936465 1102 84-85 0.3904780062573039 1102 86-87 0.3212464170251188 1102 88-89 0.3890139299727302 1102 90-91 0.3731558793632104 1102 92-93 0.5295771919035275 1102 94-95 0.5644898952279895 1102 96-97 0.5790706965937495 1102 98-99 0.8671604827552635 1102 100-101 1.137599600335225 1103 1 0.14559336365681474 1103 2 0.08070162238020373 1103 3 0.14894696964888965 1103 4 0.06179566853917606 1103 5 -0.01146772830458076 1103 6 0.035636485203767165 1103 7 0.02520407396356461 1103 8 -0.018272652714507842 1103 9 0.0642392751005616 1103 10-11 0.12032301600609685 1103 12-13 0.11405624553926685 1103 14-15 0.1362704470663587 1103 16-17 0.056373389189744216 1103 18-19 0.09657800702568409 1103 20-21 0.09971502139793387 1103 22-23 0.06374084302507299 1103 24-25 0.06425602828709032 1103 26-27 -0.019186290455628807 1103 28-29 0.05386528537340496 1103 30-31 0.08800895035388123 1103 32-33 0.06693291142160973 1103 34-35 0.08694633884751113 1103 36-37 0.004597143957354888 1103 38-39 -0.20192885781356296 1103 40-41 -0.08532343996624547 1103 42-43 0.19423686644577032 1103 44-45 0.03142689700916179 1103 46-47 0.005603859994174343 1103 48-49 0.128052094509556 1103 50-51 0.1706157806071218 1103 52-53 0.16681843052726464 1103 54-55 0.19428369001171575 1103 56-57 0.08050634467262086 1103 58-59 -0.0734044764859405 1103 60-61 0.16739499748710074 1103 62-63 0.082815702681593 1103 64-65 0.08590346142857896 1103 66-67 0.1770854150895076 1103 68-69 0.2154983225178988 1103 70-71 0.3400950242840004 1103 72-73 0.1909540409260444 1103 74-75 0.22261879920819183 1103 76-77 0.06650910515161357 1103 78-79 0.21833241427667716 1103 80-81 0.49380825522010596 1103 82-83 0.4275085432986536 1103 84-85 0.27960180468510387 1103 86-87 0.36418862927854434 1103 88-89 0.34338985216329476 1103 90-91 0.45879956012048595 1103 92-93 0.5952407926374512 1103 94-95 0.7490151908243305 1103 96-97 0.8163664276687825 1103 98-99 0.8987854271101021 1103 100-101 1.3972940770329458 1104 1 0.16315367474117437 1104 2 0.12168656735260441 1104 3 0.12415539337303727 1104 4 0.19163186871925575 1104 5 0.16314633207415596 1104 6 0.11647269937984817 1104 7 0.08343208741838026 1104 8 0.13933627113327418 1104 9 0.1381614666871016 1104 10-11 0.2321393698622245 1104 12-13 0.2580657254946672 1104 14-15 0.21520553829693512 1104 16-17 0.2948628478712294 1104 18-19 0.23083694230352592 1104 20-21 0.13298350661116132 1104 22-23 0.22902190597439898 1104 24-25 0.09268423115440783 1104 26-27 0.18868925713776008 1104 28-29 0.3922884963350697 1104 30-31 0.4200896419427522 1104 32-33 0.2602806139786793 1104 34-35 0.20720927698053515 1104 36-37 0.11541744935001219 1104 38-39 0.1910064293609679 1104 40-41 0.11185683314231198 1104 42-43 0.2232529011931561 1104 44-45 0.23959402787640016 1104 46-47 0.41304133287692224 1104 48-49 0.3478778909984328 1104 50-51 0.3192789296131693 1104 52-53 0.3016931896794759 1104 54-55 0.14557528728156655 1104 56-57 -0.044554887814882704 1104 58-59 0.01964557786518384 1104 60-61 0.23685713131683173 1104 62-63 0.2575930496936749 1104 64-65 0.35438400932521574 1104 66-67 0.38549514324058975 1104 68-69 0.008238598798886443 1104 70-71 0.062241823632717796 1104 72-73 0.14006459530749282 1104 74-75 0.2503961882082706 1104 76-77 0.08951619179149972 1104 78-79 0.16736599067382585 1104 80-81 0.14165730372556595 1104 82-83 0.17711082371312514 1104 84-85 0.18093469014652896 1104 86-87 0.3015612073878735 1104 88-89 0.42405111637506465 1104 90-91 0.5157586031675088 1104 92-93 0.7342000502897612 1104 94-95 0.7583551862523024 1104 96-97 1.183136198181085 1104 98-99 1.0801338947816816 1104 100-101 1.384266136357315 1105 1 0.09694611718007451 1105 2 0.16325322217026184 1105 3 0.06375431004789789 1105 4 0.03630507319859788 1105 5 0.12530489660646538 1105 6 0.07304343488721088 1105 7 0.12433386827675008 1105 8 0.1705832845290729 1105 9 0.09658587354321213 1105 10-11 0.14531900065434655 1105 12-13 0.04848672753603722 1105 14-15 0.12774882901737072 1105 16-17 0.29575152376821023 1105 18-19 0.19020021719624935 1105 20-21 0.14898792351944934 1105 22-23 0.10254493350925742 1105 24-25 0.1041992018195117 1105 26-27 0.10294458111426508 1105 28-29 0.14722674968987093 1105 30-31 0.14185364125243893 1105 32-33 0.02335240042693698 1105 34-35 -0.0035062635372398177 1105 36-37 0.03337461551556231 1105 38-39 0.13431165738797546 1105 40-41 0.19069183920318977 1105 42-43 0.2665058056335141 1105 44-45 0.2025175068654974 1105 46-47 0.1775277813430094 1105 48-49 0.18116091759820563 1105 50-51 0.026574529000136238 1105 52-53 0.2076375891649107 1105 54-55 0.2901424007041058 1105 56-57 0.22574044135961202 1105 58-59 0.03400534274594946 1105 60-61 0.23610287409517383 1105 62-63 0.10478858108060507 1105 64-65 0.2813637282629884 1105 66-67 0.44925738101245116 1105 68-69 0.11788092136107409 1105 70-71 0.29298402786522004 1105 72-73 0.3648023069997137 1105 74-75 0.2717522560597949 1105 76-77 0.3680952866965832 1105 78-79 0.2307514436166329 1105 80-81 0.4229065517029227 1105 82-83 0.3191226071773592 1105 84-85 0.3275837460186324 1105 86-87 0.5803778555017836 1105 88-89 0.44654407056751566 1105 90-91 0.5444169397724963 1105 92-93 0.5876170005190993 1105 94-95 0.7323566892662363 1105 96-97 0.6241459952742012 1105 98-99 0.892073573455729 1105 100-101 1.21719059856229 1106 1 -0.3463576232094283 1106 2 -0.22587993165898013 1106 3 -0.1887740663772277 1106 4 -0.22851176414113894 1106 5 -0.1340886102474954 1106 6 -0.12827869566056904 1106 7 -0.1310362958361324 1106 8 -0.19265081692317665 1106 9 -0.3569214850432445 1106 10-11 -0.4734182709555341 1106 12-13 -0.5667347149127124 1106 14-15 -0.44661164259539277 1106 16-17 -0.3977978632865842 1106 18-19 -0.33999107361119485 1106 20-21 -0.4571123320395927 1106 22-23 -0.4371543070226238 1106 24-25 -0.45671396217441895 1106 26-27 -0.36229290135884895 1106 28-29 -0.5400814458614605 1106 30-31 -0.4778359392198226 1106 32-33 -0.4590998129161932 1106 34-35 -0.9356378566180368 1106 36-37 -0.8472555231495491 1106 38-39 -0.6815531882853563 1106 40-41 -0.9109892995304065 1106 42-43 -1.1326763333487904 1106 44-45 -1.1042690454664381 1106 46-47 -0.8409586170767867 1106 48-49 -0.9722707464912475 1106 50-51 -0.9150177948569578 1106 52-53 -0.9916231279142949 1106 54-55 -1.027757545588841 1106 56-57 -0.8674135320608087 1106 58-59 -1.1296375089901503 1106 60-61 -1.279915883722083 1106 62-63 -1.1561746854613446 1106 64-65 -1.4964430056636502 1106 66-67 -1.5383669291763908 1106 68-69 -1.7693281504441671 1106 70-71 -1.6849618397559176 1106 72-73 -1.7409778689719673 1106 74-75 -1.5064589027508681 1106 76-77 -1.4520322405083768 1106 78-79 -1.4025862151165285 1106 80-81 -1.4523946943726784 1106 82-83 -1.6986342404712467 1106 84-85 -1.6043441928202142 1106 86-87 -1.3968723436468373 1106 88-89 -1.5362271410130184 1106 90-91 -1.7070600040441626 1106 92-93 -1.6438596549375752 1106 94-95 -1.4467145032104511 1106 96-97 -1.8083309088929589 1106 98-99 -1.981440561590464 1106 100-101 -1.544983419481838 1107 1 0.06724106479199321 1107 2 0.021169549008249078 1107 3 -0.04368815115479663 1107 4 0.05559502781548531 1107 5 0.040325939934973576 1107 6 0.013385385423312357 1107 7 -0.07494687899585983 1107 8 0.01131398960135499 1107 9 0.03275539098174818 1107 10-11 0.09185956838373954 1107 12-13 0.07671157145166063 1107 14-15 0.08727168113434658 1107 16-17 0.18216017639841198 1107 18-19 0.20388441949638292 1107 20-21 0.1804776093046101 1107 22-23 0.07897455842365275 1107 24-25 0.09540178328603588 1107 26-27 0.24933815813910343 1107 28-29 0.18546706705906502 1107 30-31 0.1289807651224777 1107 32-33 0.1413829523276604 1107 34-35 0.15544262149315102 1107 36-37 0.1323283457687907 1107 38-39 0.3831543453641544 1107 40-41 0.1755132195659712 1107 42-43 0.1860022860977466 1107 44-45 0.15630672688407543 1107 46-47 -0.04469921663649501 1107 48-49 -0.1759153101187465 1107 50-51 0.06864058317001565 1107 52-53 0.22908480465370218 1107 54-55 0.15038578434630523 1107 56-57 0.20338563464279247 1107 58-59 0.30186375425171974 1107 60-61 0.337341034558726 1107 62-63 0.3627373751742695 1107 64-65 0.23222556694690155 1107 66-67 0.21425448240407263 1107 68-69 0.4191205737554107 1107 70-71 0.5700689656161835 1107 72-73 0.48839989206108214 1107 74-75 0.7696621320732397 1107 76-77 0.6579348117794943 1107 78-79 0.7328447645977292 1107 80-81 0.7291516318835463 1107 82-83 0.6660348788053803 1107 84-85 0.8820349570401653 1107 86-87 0.7890606084977208 1107 88-89 0.7092514134245782 1107 90-91 0.7327660970447347 1107 92-93 0.7505990717737134 1107 94-95 0.7451091452684864 1107 96-97 0.7220404546695285 1107 98-99 0.946553912649744 1107 100-101 1.6972225258811058 1108 1 0.06489483057077905 1108 2 0.001008693521704629 1108 3 0.0838763119947501 1108 4 0.03775490400426662 1108 5 0.030105976833510795 1108 6 -0.021558202945200833 1108 7 0.06250309958200262 1108 8 0.022436742827061096 1108 9 -0.016175979481346303 1108 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0.7516055225729659 2315 88-89 1.033204502612172 2315 90-91 0.9480859252953131 2315 92-93 1.098501747101249 2315 94-95 1.1644990144546767 2315 96-97 1.5681528224294787 2315 98-99 1.186852972832103 2315 100-101 1.4166071404958203 2316 1 -0.20198532883713227 2316 2 -0.07652928230832856 2316 3 -0.23834116594433397 2316 4 -0.12331695894633299 2316 5 -0.1375518137107008 2316 6 -0.051005968387840994 2316 7 -0.007659672459503497 2316 8 -0.07100579527815398 2316 9 -0.04436737248913403 2316 10-11 -0.15967368221094347 2316 12-13 -0.05104207422007079 2316 14-15 -0.07972852571227662 2316 16-17 -0.13913985462858136 2316 18-19 -0.23263176625188464 2316 20-21 -0.07551060043785895 2316 22-23 -0.18185127671959123 2316 24-25 -0.26071829117874756 2316 26-27 -0.29746606153201327 2316 28-29 -0.2810987618787735 2316 30-31 -0.11679697818085799 2316 32-33 -0.11721669603308271 2316 34-35 -0.01691491289509628 2316 36-37 -0.08881396470798819 2316 38-39 -0.17343630516847242 2316 40-41 -0.1221364856775935 2316 42-43 -0.2676330433055014 2316 44-45 -0.16509155628894945 2316 46-47 -0.09344779456596086 2316 48-49 -0.19304996727046841 2316 50-51 0.0662592614200932 2316 52-53 -0.24692615821732744 2316 54-55 -0.2707229135542093 2316 56-57 -0.16384210348937955 2316 58-59 -0.07996218431483015 2316 60-61 -0.08770809151429404 2316 62-63 0.0010763967897347015 2316 64-65 -0.004668113888754988 2316 66-67 -0.15416779497725486 2316 68-69 0.0674683863523704 2316 70-71 0.1690424892484117 2316 72-73 0.05512602713192649 2316 74-75 0.029471833179869122 2316 76-77 0.3132274997513633 2316 78-79 0.3987124861821698 2316 80-81 0.3711983792203952 2316 82-83 0.35331381147680574 2316 84-85 0.6494436859676647 2316 86-87 0.6406817689072781 2316 88-89 0.7537079239220468 2316 90-91 0.5132863163021533 2316 92-93 0.5138243277464092 2316 94-95 0.3906231591272089 2316 96-97 0.46937471881266646 2316 98-99 0.3282996981497952 2316 100-101 0.7591291346307187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 10.0 5 21.0 6 69.0 7 150.0 8 336.0 9 665.0 10 1235.0 11 2013.0 12 3434.0 13 5219.0 14 6409.0 15 9196.0 16 13007.0 17 19326.0 18 17605.0 19 9105.0 20 6824.0 21 6581.0 22 6593.0 23 7059.0 24 7898.0 25 9178.0 26 10729.0 27 13023.0 28 15747.0 29 19508.0 30 23830.0 31 29990.0 32 38275.0 33 49877.0 34 68988.0 35 97863.0 36 143603.0 37 213352.0 38 164243.0 39 14055.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.329802297428564 19.877251397835952 15.30162811039642 19.491318194339065 2 15.365909036013623 22.09492524335161 40.54626699838168 21.992898722253084 3 19.79720296611193 28.112461051665417 30.495785125963142 21.594550856259513 4 12.20086471341272 17.9279727542813 37.66527378565735 32.20588874664863 5 12.247315537081 39.64695435567576 33.04278424677058 15.062945860472665 6 28.53061520253134 37.56788483369967 18.166807565034663 15.734692398734328 7 26.56071109393493 35.9529479964252 20.29835028139417 17.187990628245693 8 26.73829134561967 34.17627593536388 19.644066568440376 19.441366150576073 9 27.918069611845127 16.814279848312843 20.873119006787274 34.39453153305475 10-11 24.644815342624575 28.151252385208085 26.081978696166757 21.12195357600058 12-13 26.9541406554206 26.002952745444553 27.497876747999218 19.545029851135638 14-15 23.718895678848337 25.901789811840292 28.33743146300814 22.04188304630323 16-17 20.10004589261129 29.74802541001425 28.91277988454385 21.239148812830607 18-19 19.979420786937514 27.126929301224607 33.52508393517065 19.368565976667227 20-21 21.482635151716583 25.804717194417126 33.51436008123499 19.198287572631305 22-23 20.963845294323104 25.8997372490424 33.1291325063852 20.007284950249296 24-25 20.50385256394773 26.017390884278157 33.79585034177918 19.682906209994925 26-27 20.639459331507272 26.102327019415178 33.773037105742425 19.48517654333512 28-29 19.96630945542959 26.049399594302848 34.36039414169063 19.623896808576934 30-31 19.98652206468443 26.564672350909397 34.06782444868482 19.38098113572136 32-33 19.917779763773822 26.551725803724548 33.56088017197652 19.96961426052511 34-35 20.44399893722374 26.1288857757059 33.605082002850175 19.82203328422019 36-37 20.7698000622209 26.092875320524588 33.50426367748705 19.633060939767464 38-39 20.821091277988454 26.132267336537762 33.59121760343953 19.455423782034252 40-41 20.34487172984162 26.343195437780338 33.73216524278458 19.579767589593462 42-43 20.133578980275285 26.847171750408407 33.10285813405926 19.916391135257054 44-45 20.315596241636673 27.172483756431003 32.25405183449675 20.257868167435568 46-47 20.484867515277408 27.350933552329654 31.370256757083165 20.793942175309777 48-49 20.935388034105458 27.443684935146496 31.051955266780993 20.568971763967053 50-51 21.366053960049275 27.876041641506244 30.484770899253643 20.27313349919084 52-53 21.1272776634756 28.253437479016196 30.34691449332842 20.272370364179785 54-55 20.495688509939374 28.21009154368252 30.53418999541074 20.76002995096737 56-57 21.077172048984323 28.670225356875438 29.731552377961883 20.521050216178352 58-59 21.05780053621893 27.912562498490374 30.08888674186614 20.940750223424555 60-61 21.110118113089055 27.87763580589841 30.49766913842661 20.514576942585926 62-63 21.638849303156928 27.62682060819787 30.45105190695877 20.28327818168643 64-65 21.20127533151373 27.585903722132315 30.640709161614453 20.572111784739498 66-67 20.83592183763677 28.122943890244194 30.350088162121686 20.691046109997345 68-69 20.952778918383615 28.379024661240067 30.10424868964518 20.56394773073114 70-71 20.920364242409605 28.485640443467545 29.702857418902923 20.89113789521992 72-73 21.083355474505446 28.392840752638826 29.40532837371078 21.11847539914495 74-75 21.058428540373423 28.577570590082363 29.39967633632038 20.964324533223834 76-77 21.221709620540565 28.069611845124516 29.991207941837157 20.717470592497765 78-79 21.021472911282334 28.17844979589865 29.948165503248713 20.851911789570302 80-81 20.80630902635202 29.01041037656095 29.51445617255622 20.668824424530808 82-83 20.879602366900492 28.886369356474173 29.166894116999163 21.06713415962617 84-85 20.855778497568895 28.361943822477286 29.147675533623662 21.634602146330153 86-87 21.02654525253013 28.259027559720778 29.49870776068211 21.21571942706698 88-89 21.44325016303954 27.742131832564432 29.619236250332115 21.19538175406391 90-91 20.806878166286126 28.22894754895756 29.830074971471415 21.1340993132849 92-93 20.741369115481678 28.54259500446375 29.588098210248464 21.12793766980611 94-95 20.4786357817444 28.502113475519913 29.292094393855223 21.72715634888046 96-97 20.759112098741575 28.22419748315258 28.919543006207583 22.097147411898263 98-99 20.695657046737676 28.186660856803456 29.024952621757134 22.092729474701734 100-101 20.871573816936547 28.34994366594582 29.358497165904275 21.419985351213356 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1728.0 1 1181.5 2 1404.0 3 1818.0 4 1171.5 5 923.5 6 1390.5 7 2715.5 8 4057.0 9 4538.5 10 4036.0 11 3320.5 12 3199.5 13 3367.0 14 3574.5 15 3672.5 16 3689.0 17 3791.5 18 3943.0 19 4059.0 20 4262.0 21 4582.0 22 5080.5 23 5708.0 24 6400.5 25 7336.0 26 8684.0 27 10070.5 28 11080.0 29 12519.5 30 14332.5 31 15700.5 32 17272.0 33 19838.0 34 22704.0 35 24516.5 36 27233.5 37 30117.5 38 31252.5 39 31876.0 40 31955.0 41 32477.0 42 33193.0 43 34321.5 44 35965.5 45 36225.5 46 34966.5 47 36037.0 48 47840.5 49 47855.0 50 37155.5 51 33172.5 52 28390.5 53 26153.5 54 24693.5 55 22442.0 56 19957.5 57 17924.5 58 16334.5 59 14754.5 60 12772.0 61 10437.5 62 8235.5 63 6067.0 64 4372.5 65 3422.0 66 2521.0 67 1821.5 68 1394.5 69 1042.0 70 767.0 71 608.0 72 535.5 73 489.0 74 397.5 75 339.5 76 258.5 77 156.5 78 103.5 79 70.5 80 47.0 81 29.5 82 14.5 83 9.5 84 7.0 85 1.5 86 2.0 87 2.0 88 0.5 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03768024926934132 2 0.0 3 0.0 4 0.0 5 0.008502210091543682 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005410497330982343 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.898480713026256E-4 22-23 0.0014009323446293569 24-25 0.0 26-27 9.661602376754185E-4 28-29 0.0015941643921644404 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.898480713026256E-4 38-39 0.0 40-41 2.4154005941885462E-4 42-43 0.01995120890799739 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.830801188377092E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.830801188377092E-5 84-85 2.4154005941885462E-4 86-87 0.0 88-89 0.0 90-91 0.0094683703292191 92-93 0.002028936499118379 94-95 0.0 96-97 0.0 98-99 3.3815608318639646E-4 100-101 0.012270235018477816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.81739051418759 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12371544724883 41.26290787196611 2 12.630556899802578 13.342261123770788 3 3.9195518820084665 6.210615071779803 4 1.6773135466991334 3.5436529844298055 5 0.9111697574169785 2.406280445110506 6 0.5331271488250833 1.6895030887925897 7 0.3465992165167826 1.2814526319474855 8 0.2730463182346839 1.1537275214929952 9 0.1968200174972058 0.9355967752643236 >10 1.21349809110036 12.044048178069415 >50 0.11481810122070707 4.144491182716973 >100 0.05391524743716873 5.303529490211396 >500 0.002567392621530509 0.9444747466080332 >1k 0.002750777808782688 2.78451030822354 >5k 1.833851872521792E-4 0.7216015651963745 >10k+ 3.667703745043584E-4 2.231347014419984 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12569 1.2143668027342336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10468 1.011376536798628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7450 0.7197893770681868 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4350 0.42027970338880705 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3871 0.37400062800415446 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2654 0.25641892707905606 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2383 0.2302359846380522 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2317 0.2238593270693945 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.19294219946378108 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1959 0.18927079056061447 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1433 0.13845076205888746 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1305 0.1260839110166421 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1176 0.1136204439506292 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1114 0.10763025047704161 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1060 0.10241298519359436 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1045 0.10096374483708123 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1043 0.10077051278954614 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1041 0.10057728074201107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.8308011883770923E-5 0.0 18-19 0.0 0.0 0.0 9.661602376754185E-5 0.0 20-21 0.0 0.0 0.0 9.661602376754185E-5 0.0 22-23 0.0 0.0 0.0 5.796961426052511E-4 0.0 24-25 0.0 0.0 0.0 6.763121663727929E-4 0.0 26-27 0.0 0.0 0.0 9.178522257916476E-4 0.0 28-29 0.0 0.0 0.0 0.0017873964396995243 0.0 30-31 0.0 0.0 0.0 0.0036714089031665903 0.0 32-33 0.0 0.0 0.0 0.005507113354749885 0.0 34-35 0.0 0.0 0.0 0.007536049853868264 0.0 36-37 0.0 0.0 0.0 0.01091761068573223 0.0 38-39 0.0 0.0 0.0 0.017101036206854908 0.0 40-41 0.0 0.0 0.0 0.024926934132025798 0.0 42-43 0.0 0.0 0.0 0.03086881959372962 0.0 44-45 0.0 0.0 0.0 0.033429144223569476 0.0 46-47 0.0 0.0 0.0 0.03869471751890051 0.0 48-49 0.0 0.0 0.0 0.04729354363421173 0.0 50-51 0.0 0.0 0.0 0.05719668607038477 0.0 52-53 0.0 0.0 0.0 0.07603681070505544 0.0 54-55 0.0 0.0 0.0 0.09096398637714065 0.0 56-57 0.0 0.0 0.0 0.12304050626796455 0.0 58-59 0.0 0.0 0.0 0.1715900582111543 0.0 60-61 0.0 0.0 0.0 0.21757928552450423 0.0 62-63 0.0 0.0 0.0 0.2510084297480737 0.0 64-65 0.0 0.0 0.0 0.3016835342141494 0.0 66-67 0.0 0.0 0.0 0.37559479239631893 0.0 68-69 0.0 0.0 0.0 0.4794570179464265 0.0 70-71 0.0 0.0 0.0 0.5895992850414241 0.0 72-73 0.0 0.0 0.0 0.7078089901210116 0.0 74-75 0.0 0.0 0.0 0.8409941788845681 0.0 76-77 0.0 0.0 0.0 0.9530687664549164 0.0 78-79 0.0 0.0 0.0 1.0907949083355475 0.0 80-81 0.0 0.0 0.0 1.2613704982971425 0.0 82-83 0.0 0.0 0.0 1.4335402526509022 0.0 84-85 0.0 0.0 0.0 1.638945919180696 0.0 86-87 0.0 0.0 0.0 1.8837709234076472 0.0 88-89 0.0 0.0 0.0 2.1717832902586895 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 100 1.2732926E-11 47.49884 2 TACGGGC 50 0.0016147852 38.00458 7 ACCGTAT 55 0.0025779835 34.549618 8 TACCTGG 995 0.0 31.984142 2 ATCACGC 155 1.3569661E-9 30.644413 3 GTACCTG 1055 0.0 30.17826 1 GGACCGA 205 1.8189894E-12 30.11976 6 TCACGCA 170 3.6416168E-9 27.940495 4 GATATCG 70 0.008386259 27.14613 9 ACCTGGG 1330 0.0 23.927986 3 CCCGCGA 50 0.0016543267 23.74942 20-21 AGTACTT 9260 0.0 22.829424 12-13 GAGTACT 9100 0.0 22.604374 12-13 GTATCAA 16330 0.0 22.232027 1 GGTATCA 12375 0.0 22.041368 1 GTACATG 10400 0.0 21.795004 1 AGGACCG 285 8.367351E-11 21.666138 5 CATGGGG 2445 0.0 21.56389 4 TACATGG 10445 0.0 21.37334 2 GTACTTT 9820 0.0 20.918795 14-15 >>END_MODULE