FastQCFastQC Report
Thu 26 May 2016
SRR937532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913822
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97531.0672756838859208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76170.8335321320782385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.604712952850774No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36090.394934680933486No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29730.3253368817997378No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22000.24074710392177034No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.19817863872833003No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.1939108491588077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.1525461194849763No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13780.15079523145645432No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13550.148278329915454No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12490.136678696726496No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11960.13087888013201696No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11660.12759596507853826No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.10155150565427404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG853.6245365E-739.1383252
ATAACGC1201.4982834E-731.6834093
TAACGCA1102.7213955E-630.2432524
TACCTGG8250.029.3791582
GTACCTG8450.029.259071
GTATCAA120650.025.5759181
GTACATG87800.024.6935861
TACATGG87550.024.2103922
GGTATCA88250.023.7056481
GAGTACT65950.023.47943512-13
AGTACTT67750.023.1010112-13
ACCTGGG10450.022.7392883
GGTGCGT1050.002193333822.6235546
ACATGGG88650.022.6233463
GTACACG2005.333677E-721.3956951
CATGGGA64450.021.2369774
GTACTTT73200.021.21708914-15
ATCAACG144050.021.2139153
TATCAAC144750.021.176992
TCAACGC145300.020.9987054
Produced by FastQC (version 0.11.5)