FastQCFastQC Report
Thu 26 May 2016
SRR937532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913822
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109551.1988111470286336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92451.0116849889803485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64840.7095473735585267No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40660.4449444202481446No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34930.38224074272670167No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.2608823162497729No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21870.23932450739859623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.23221152478272575No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18470.20211813679250445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.19489572367485133No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13290.1454331368691058No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.13503723919975663No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10830.11851323343058057No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC9670.10581929522379632No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9510.10406840719527435No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9400.10286467167566549No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT9320.10198922766140452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG702.0513652E-433.9331862
AATACCG702.0513652E-433.9331865
TACCTGG8250.031.0951352
GTACCTG8950.030.7997911
TATCACG650.005839572829.2347432
GGACCGA1405.805341E-727.142096
TATACCG950.001225390725.0033995
GTATCAA145500.023.2246651
GTGTAGC4150.022.9047051
GAGTACT81550.022.3672412-13
ACCTGGG10950.022.1262933
GGTATCA110200.021.8659921
AGTACTT82700.021.53926512-13
GTACATG85450.021.358071
GACGAGT1353.7958456E-421.1105186
GTACTTT87100.021.02265714-15
TACATGG86700.020.8217472
GGAACGC1404.8498245E-420.3576838
CGGTTAC704.8532736E-420.35712428-29
ACATGGG85950.020.1190813
Produced by FastQC (version 0.11.5)