##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937532_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 913822 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19888665407486 33.0 31.0 34.0 31.0 34.0 2 32.42285259054827 34.0 31.0 34.0 31.0 34.0 3 32.497545473845015 34.0 31.0 34.0 31.0 34.0 4 36.124252863249076 37.0 35.0 37.0 35.0 37.0 5 35.98260602174165 37.0 35.0 37.0 35.0 37.0 6 36.10040576830061 37.0 35.0 37.0 35.0 37.0 7 36.06144522675094 37.0 35.0 37.0 35.0 37.0 8 36.07355590038323 37.0 35.0 37.0 35.0 37.0 9 37.72068083280989 39.0 37.0 39.0 35.0 39.0 10-11 37.67535417181902 39.0 37.0 39.0 35.0 39.0 12-13 37.58323776402844 39.0 37.0 39.0 35.0 39.0 14-15 38.710897198798015 40.0 38.0 41.0 34.5 41.0 16-17 38.69450067956342 40.0 38.0 41.0 34.5 41.0 18-19 38.77474059499552 40.0 38.0 41.0 35.0 41.0 20-21 38.74227913094673 40.0 38.0 41.0 35.0 41.0 22-23 38.58599377121584 40.0 38.0 41.0 34.0 41.0 24-25 38.51055676050697 40.0 38.0 41.0 34.0 41.0 26-27 38.381333563866924 40.0 38.0 41.0 34.0 41.0 28-29 38.250269746186895 40.0 38.0 41.0 34.0 41.0 30-31 38.20364359798735 40.0 37.0 41.0 34.0 41.0 32-33 38.012064712821534 40.0 37.0 41.0 34.0 41.0 34-35 37.83080731258386 40.0 36.0 41.0 33.0 41.0 36-37 37.61732591248624 40.0 36.0 41.0 33.0 41.0 38-39 37.43001043966987 40.0 36.0 41.0 33.0 41.0 40-41 37.186209677595855 40.0 36.0 41.0 33.0 41.0 42-43 36.90999614804634 40.0 35.5 41.0 32.0 41.0 44-45 36.46738423894369 39.5 35.0 41.0 31.0 41.0 46-47 35.88760612022911 39.0 35.0 41.0 29.5 41.0 48-49 35.36399867807954 39.0 35.0 41.0 26.5 41.0 50-51 35.02966277896571 39.0 35.0 41.0 24.5 41.0 52-53 34.754514008198534 39.0 35.0 40.0 23.5 41.0 54-55 34.539140554725094 38.5 35.0 40.0 23.0 41.0 56-57 34.28374781959725 38.0 34.0 40.0 22.5 41.0 58-59 34.033838646913736 38.0 34.0 40.0 21.5 41.0 60-61 33.69238210504891 38.0 34.0 40.0 19.5 41.0 62-63 33.3079434506939 37.0 33.0 40.0 18.0 41.0 64-65 32.89163480415223 36.5 33.0 39.5 14.0 41.0 66-67 32.47400916152161 36.0 32.0 39.0 10.5 40.5 68-69 32.03349448798562 35.5 32.0 39.0 8.0 40.0 70-71 31.63020150532598 35.0 31.0 38.0 7.0 40.0 72-73 31.177662061101614 35.0 31.0 37.5 2.0 39.5 74-75 30.68037320178328 35.0 30.5 37.0 2.0 39.0 76-77 29.469721674461766 33.5 29.0 35.5 2.0 38.0 78-79 29.98470708737588 34.0 30.0 36.0 2.0 38.5 80-81 29.781547172206402 34.0 30.0 36.0 2.0 37.5 82-83 29.429405288995014 34.0 30.0 35.0 2.0 37.0 84-85 29.027902589344535 34.0 29.0 35.0 2.0 36.5 86-87 28.582804966393894 34.0 29.0 35.0 2.0 36.0 88-89 28.117101579957584 34.0 28.5 35.0 2.0 36.0 90-91 27.56995891978963 33.0 27.0 35.0 2.0 35.0 92-93 27.14078507630589 33.0 26.0 35.0 2.0 35.0 94-95 26.686158792412527 33.0 25.0 35.0 2.0 35.0 96-97 26.128336262423097 33.0 24.5 35.0 2.0 35.0 98-99 25.329813136475153 32.0 21.0 34.0 2.0 35.0 100-101 23.033319946335283 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10459776751011418 1101 2 0.07933754937582904 1101 3 0.09500030388313974 1101 4 0.0653226704966201 1101 5 -0.11284613411329758 1101 6 0.015754871757714284 1101 7 -0.021066788737208242 1101 8 -0.01117982447893695 1101 9 0.06922673957443948 1101 10-11 0.027712363950591623 1101 12-13 0.07399078863900854 1101 14-15 0.12850869308248036 1101 16-17 -0.043734748891289144 1101 18-19 0.06892712458179062 1101 20-21 0.010337604684195867 1101 22-23 0.0871836086447928 1101 24-25 0.004940741674012372 1101 26-27 -0.06305648102046035 1101 28-29 -0.08654360331747313 1101 30-31 0.0865117548431229 1101 32-33 0.20020921896139754 1101 34-35 0.09036721371057865 1101 36-37 0.14774622806604043 1101 38-39 0.26657309193646483 1101 40-41 0.26810307435413705 1101 42-43 0.07616002792131837 1101 44-45 0.10947140221762908 1101 46-47 0.21220783315354907 1101 48-49 0.18511268477276843 1101 50-51 0.1481836451125389 1101 52-53 0.12474413946952723 1101 54-55 0.23510580266771086 1101 56-57 0.13915981292472424 1101 58-59 0.26947706601759336 1101 60-61 0.24310857544750064 1101 62-63 0.34697807131805547 1101 64-65 0.025819641919959224 1101 66-67 0.3592804218013157 1101 68-69 0.3260757650228001 1101 70-71 0.3008083189096169 1101 72-73 0.2501686153675706 1101 74-75 -0.012821908838905216 1101 76-77 -0.039807127365708794 1101 78-79 0.20086070748639173 1101 80-81 0.14306803220603825 1101 82-83 0.07537144324454914 1101 84-85 0.4001216453711436 1101 86-87 0.38100502438704353 1101 88-89 0.46335763478024816 1101 90-91 0.5405155474343637 1101 92-93 0.6694397128663852 1101 94-95 0.6065836722093714 1101 96-97 0.7689373506450643 1101 98-99 1.0111916360796904 1101 100-101 1.5200916992829292 1102 1 0.015629258010804392 1102 2 -0.03923005339422048 1102 3 0.0202932929230073 1102 4 -0.07354818793010054 1102 5 -0.18687109209395913 1102 6 -0.08285265117201845 1102 7 -0.06114671502963631 1102 8 -0.07191549599305347 1102 9 -0.15645255510253975 1102 10-11 -0.05989956223294257 1102 12-13 -0.09115916987898487 1102 14-15 -0.132415617664293 1102 16-17 -0.011068566539556457 1102 18-19 -0.17069413541079115 1102 20-21 -0.06321306855654285 1102 22-23 0.0017460845544192694 1102 24-25 -0.15110896795196282 1102 26-27 -0.21398792753159768 1102 28-29 -0.2072017844426597 1102 30-31 -0.10696991068439843 1102 32-33 -0.05103273360153082 1102 34-35 -0.11130553555040734 1102 36-37 -0.11038610740659038 1102 38-39 0.009275769854141913 1102 40-41 -0.04591390246035587 1102 42-43 0.002881244497494606 1102 44-45 0.23542444946125585 1102 46-47 0.1765462059413565 1102 48-49 0.14665155964326715 1102 50-51 0.4479806770357726 1102 52-53 0.44777324278479114 1102 54-55 0.5034918589829331 1102 56-57 0.5156405198525817 1102 58-59 0.4844186831422874 1102 60-61 0.5512865482886085 1102 62-63 0.3569570365749044 1102 64-65 0.4192141164310925 1102 66-67 0.5855622443729729 1102 68-69 0.4271865370179313 1102 70-71 0.5170649657021791 1102 72-73 0.46580267388370444 1102 74-75 0.46368093615308936 1102 76-77 0.30149984949177266 1102 78-79 0.4909166329649395 1102 80-81 0.6909650594007495 1102 82-83 0.600422919617003 1102 84-85 0.6305086817793004 1102 86-87 0.6070948404476724 1102 88-89 0.42235708025398466 1102 90-91 0.4528960986076811 1102 92-93 0.7878378770548764 1102 94-95 0.6810273906590467 1102 96-97 0.8336292160169734 1102 98-99 1.147112901947093 1102 100-101 1.7955798471795248 1103 1 0.1448833799757594 1103 2 0.23851028282164322 1103 3 0.1984100572419436 1103 4 0.10252356507914584 1103 5 -0.08311467379121495 1103 6 0.08815396053979896 1103 7 0.0989489575214435 1103 8 0.10997888791306565 1103 9 0.19690265346334002 1103 10-11 0.13716391946726247 1103 12-13 0.21268191315563456 1103 14-15 0.2100662103076374 1103 16-17 0.12570639516474813 1103 18-19 0.1319921094486034 1103 20-21 0.16241914391682855 1103 22-23 0.16108812866713862 1103 24-25 0.10235028135408442 1103 26-27 0.12228831256803119 1103 28-29 0.18388117997428566 1103 30-31 0.1852406531714763 1103 32-33 0.299720052401689 1103 34-35 0.3503124173041172 1103 36-37 0.12051939804747747 1103 38-39 0.1114645567615753 1103 40-41 0.24796994819835305 1103 42-43 0.3975891371319449 1103 44-45 0.32944828475957166 1103 46-47 0.5424368494354397 1103 48-49 0.7224591390322459 1103 50-51 0.5438578696487397 1103 52-53 0.5543240886598824 1103 54-55 0.40623307273541087 1103 56-57 0.5615212928149091 1103 58-59 0.6197492195909433 1103 60-61 0.606751425061411 1103 62-63 0.5603043824127809 1103 64-65 0.595429434429299 1103 66-67 0.4476570155184376 1103 68-69 0.39232657079958244 1103 70-71 0.6005250153216117 1103 72-73 0.7051630690419515 1103 74-75 0.8853730794625392 1103 76-77 0.7429755290912219 1103 78-79 0.6366810796745987 1103 80-81 0.6489703695502662 1103 82-83 0.6804468868968208 1103 84-85 0.717337769941544 1103 86-87 0.9324159135415577 1103 88-89 1.0149280659452202 1103 90-91 0.9088198919246437 1103 92-93 0.9347982306529872 1103 94-95 0.7369527986433653 1103 96-97 0.8686962808746514 1103 98-99 0.8712238169580253 1103 100-101 1.280674712396923 1104 1 0.06420124962151874 1104 2 0.08545640410335409 1104 3 0.02783379656164442 1104 4 -0.046477259056040054 1104 5 -0.20777392558916574 1104 6 -0.16432865866043755 1104 7 0.010262150023926608 1104 8 0.0037147561077901514 1104 9 0.02187606277677645 1104 10-11 -0.05453239023851353 1104 12-13 -0.06474770076872716 1104 14-15 -0.06953890919196226 1104 16-17 -0.07939619910011686 1104 18-19 -0.11488005098304654 1104 20-21 -0.08151817954566098 1104 22-23 -0.016001617541490987 1104 24-25 -0.0746320455247016 1104 26-27 -0.12515581177072477 1104 28-29 -0.08094303973874872 1104 30-31 -0.05030704453291435 1104 32-33 -0.0638123177970158 1104 34-35 -0.10681740864134781 1104 36-37 -0.04325915612356113 1104 38-39 -0.19060247290025245 1104 40-41 -0.26951580553314614 1104 42-43 -0.26388676924066345 1104 44-45 -0.31442282613239314 1104 46-47 0.0845547359865364 1104 48-49 0.079637920016566 1104 50-51 0.06270837716104438 1104 52-53 0.13639562641161262 1104 54-55 0.039526373291174366 1104 56-57 0.26601282958048955 1104 58-59 0.22458449821203175 1104 60-61 0.027066709599360195 1104 62-63 0.005590258707975693 1104 64-65 0.14349940843734288 1104 66-67 0.24389522580674594 1104 68-69 0.14411783214904972 1104 70-71 0.3210745092180751 1104 72-73 0.22145629211491524 1104 74-75 0.4248699343654394 1104 76-77 -0.07268339164387427 1104 78-79 0.22628464951829486 1104 80-81 0.31121828631966153 1104 82-83 0.39023331613741874 1104 84-85 0.3329023025441913 1104 86-87 0.5956932949048337 1104 88-89 0.6343308144712871 1104 90-91 0.36900394878292886 1104 92-93 0.5817830933323407 1104 94-95 0.7195786905759967 1104 96-97 0.8721502525723004 1104 98-99 1.015564403150588 1104 100-101 1.578666147026098 1105 1 0.017436619173288648 1105 2 0.019600905710099426 1105 3 0.004551808721629413 1105 4 -0.005066942685324705 1105 5 -0.19628530859838378 1105 6 -0.1772233677110222 1105 7 -0.004117102574781484 1105 8 -0.11679879940418658 1105 9 0.06187877840521594 1105 10-11 -0.00851832931791563 1105 12-13 0.032353365869781214 1105 14-15 -0.0022848621512423506 1105 16-17 -0.09428010598423242 1105 18-19 -0.02538425266372002 1105 20-21 0.10809605034736336 1105 22-23 0.023832879991815048 1105 24-25 -0.19675461088836954 1105 26-27 -0.13480987088090046 1105 28-29 -0.08605445593898509 1105 30-31 -0.05663219577833445 1105 32-33 -0.09425903867567342 1105 34-35 -0.13211575059931846 1105 36-37 -0.2220365198518195 1105 38-39 -0.222862630104963 1105 40-41 -0.13947341155580517 1105 42-43 -0.37376034164987715 1105 44-45 -0.16616007399384358 1105 46-47 -0.27533618627105483 1105 48-49 -0.2576793408195428 1105 50-51 -0.026418548294955713 1105 52-53 0.07722811739723312 1105 54-55 0.07227232625044877 1105 56-57 0.27808304366253367 1105 58-59 -0.05961507030477975 1105 60-61 -0.011040346508544019 1105 62-63 0.15720002290090918 1105 64-65 0.22082089358436718 1105 66-67 0.24115017805694805 1105 68-69 0.09726268075276323 1105 70-71 0.21493492591617525 1105 72-73 0.43039523573545324 1105 74-75 0.30416402183885083 1105 76-77 0.1667950568270733 1105 78-79 0.30488323437414167 1105 80-81 0.4477216129645001 1105 82-83 0.4518479081142459 1105 84-85 0.6659379225372497 1105 86-87 0.6085698997658042 1105 88-89 0.6279000554531358 1105 90-91 0.6013402944522532 1105 92-93 0.9286782248140177 1105 94-95 0.9014232057608886 1105 96-97 0.7252815230338037 1105 98-99 1.091588421375473 1105 100-101 1.6537072586233386 1106 1 -0.09016438047807185 1106 2 0.01491544724873961 1106 3 -0.15449474283959574 1106 4 0.014411270584488989 1106 5 0.0299069906974907 1106 6 -0.02114514205960205 1106 7 -0.021708750859119164 1106 8 -0.03198809127435709 1106 9 0.08842427915951845 1106 10-11 0.07431050808234119 1106 12-13 -0.0463479996904681 1106 14-15 -0.029479582375941504 1106 16-17 -0.06018590155097314 1106 18-19 0.16381719924190463 1106 20-21 0.12234873927267387 1106 22-23 0.12538074302523938 1106 24-25 -0.040697564291185984 1106 26-27 -0.15152421757224488 1106 28-29 -0.04231807977291169 1106 30-31 -0.16154101180403302 1106 32-33 -0.09983238825714125 1106 34-35 -0.1599987900868456 1106 36-37 -0.12693360593979008 1106 38-39 -0.173271874599223 1106 40-41 -0.04771434447685152 1106 42-43 -0.06648403319161389 1106 44-45 -0.05252467304175923 1106 46-47 0.048318862326730994 1106 48-49 -0.015928582446491646 1106 50-51 0.08146266653560019 1106 52-53 0.0848827163474084 1106 54-55 -0.1641445687698635 1106 56-57 -0.2618639109523784 1106 58-59 -0.16273344166809522 1106 60-61 -0.015316886307239486 1106 62-63 -0.03724324492941378 1106 64-65 -0.11010671640823944 1106 66-67 0.03571986854951348 1106 68-69 -0.24848537973461404 1106 70-71 -0.006763340985525446 1106 72-73 -0.016284866054341762 1106 74-75 -0.06014585053291199 1106 76-77 -0.07453945167224063 1106 78-79 0.056272906163965075 1106 80-81 0.2860928459438732 1106 82-83 -0.020620135951222807 1106 84-85 0.1323357238255909 1106 86-87 0.2535918445243155 1106 88-89 0.3417954700249801 1106 90-91 0.6356291832687369 1106 92-93 0.7404518239824966 1106 94-95 0.5427779158196344 1106 96-97 0.7703994251996065 1106 98-99 0.9284355770284911 1106 100-101 1.730763174584851 1107 1 -0.03476655667908091 1107 2 0.07408308765348437 1107 3 -0.019125261033991592 1107 4 0.10116097431950521 1107 5 -0.21667995375386084 1107 6 -0.10561256948503939 1107 7 -0.10185941092922235 1107 8 -0.10205248229442532 1107 9 0.05973339749148465 1107 10-11 -0.043391221442227845 1107 12-13 -0.017406846618847283 1107 14-15 0.03242571227306712 1107 16-17 -0.045698336393392935 1107 18-19 -0.03063539421623318 1107 20-21 -0.01367542600589644 1107 22-23 -0.02344952821260904 1107 24-25 -0.08781074166068237 1107 26-27 -0.048163370958782536 1107 28-29 -0.2029682881942918 1107 30-31 -0.1945378875550574 1107 32-33 -0.18038497425686728 1107 34-35 -0.106454797843611 1107 36-37 -0.26238927392410716 1107 38-39 -0.22386481893455112 1107 40-41 -0.1297130351099085 1107 42-43 0.0917077973531022 1107 44-45 0.09421268286572371 1107 46-47 0.07220486453110908 1107 48-49 -0.006402523558129758 1107 50-51 -0.1485786033845926 1107 52-53 -0.13542241587379067 1107 54-55 -0.14074875045695734 1107 56-57 0.05385097853870491 1107 58-59 -0.12655513572373422 1107 60-61 0.027350658795477045 1107 62-63 -0.16291056627954958 1107 64-65 -0.07689313214052618 1107 66-67 -0.028202928523477055 1107 68-69 -0.1579331252202003 1107 70-71 0.0775362720053856 1107 72-73 -0.009413590084538015 1107 74-75 0.20595853418893384 1107 76-77 0.15571728369475224 1107 78-79 0.33502759875278443 1107 80-81 0.2536437496556765 1107 82-83 0.19849662145955094 1107 84-85 0.5255982850467795 1107 86-87 0.5296482467485859 1107 88-89 0.4646232054002617 1107 90-91 0.608413411198029 1107 92-93 0.6029488198969375 1107 94-95 0.42411612198691984 1107 96-97 0.5922281467436683 1107 98-99 1.0544807916237886 1107 100-101 1.7189581542399424 1108 1 0.03337495788598943 1108 2 0.06714902575034642 1108 3 0.03853547335424423 1108 4 0.06121612300592716 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-0.5714411447166725 2316 52-53 -0.35095586590945516 2316 54-55 -0.41606620856915555 2316 56-57 -0.3915212185215111 2316 58-59 -0.38681799381926396 2316 60-61 -0.5341329426753418 2316 62-63 -0.3124914567368222 2316 64-65 -0.15454463676694274 2316 66-67 -0.3062786368417605 2316 68-69 -0.2069832979581676 2316 70-71 -0.4321129726712307 2316 72-73 -0.3578830298207549 2316 74-75 -0.12924481498257023 2316 76-77 -0.3485599491634339 2316 78-79 -0.31580459997460864 2316 80-81 -0.10287907676350372 2316 82-83 -0.3448434731990062 2316 84-85 -0.18985921585627352 2316 86-87 -0.37417051268717216 2316 88-89 -0.32111688184862075 2316 90-91 -0.4002948052014226 2316 92-93 -0.023693580095653033 2316 94-95 -0.5907226713480824 2316 96-97 -0.6311966034114711 2316 98-99 -0.7699470428071038 2316 100-101 -1.6984564261410995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 13.0 5 17.0 6 52.0 7 146.0 8 270.0 9 503.0 10 1015.0 11 1677.0 12 2830.0 13 4221.0 14 5386.0 15 7596.0 16 11155.0 17 16455.0 18 15081.0 19 7763.0 20 5464.0 21 5248.0 22 5404.0 23 5766.0 24 6296.0 25 7305.0 26 8884.0 27 10442.0 28 13169.0 29 16185.0 30 20187.0 31 25641.0 32 32576.0 33 43016.0 34 59544.0 35 86811.0 36 128266.0 37 193636.0 38 152535.0 39 13260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.398903299188696 19.817464670316497 15.300388519782324 19.483243510712484 2 15.39150950622769 22.031642923895465 40.62530777328626 21.951539796590584 3 19.767088120005866 28.189625550709003 30.554528124733267 21.48875820455187 4 12.110017049272177 17.9979251976862 37.73481049920006 32.15724725384156 5 12.247694473083397 39.71799569649569 33.08762206213595 14.946687768284969 6 28.647045048160365 37.48257319259112 18.155723981256745 15.714657777991775 7 26.586140408088227 35.89594034724487 20.430455821812124 17.08746342285478 8 26.742626025637378 34.17755317775234 19.636428100877414 19.443392695732868 9 27.88880110130857 16.91434436903467 20.860517693817833 34.33633683583893 10-11 24.596310878923905 28.20675142423798 26.083307252397077 21.11363044444104 12-13 26.972671938506387 26.036840135436524 27.45604550737262 19.53444241868446 14-15 23.664729017248437 25.89705653836305 28.35869567596315 22.07951876842536 16-17 20.064191932345686 29.788076890247773 28.96061815101847 21.187113026388072 18-19 19.951040793502457 27.204805749916282 33.51380246918984 19.330350987391416 20-21 21.411250124477334 25.846035492727786 33.56417722945741 19.17853715333747 22-23 20.873084137103888 26.031824717832215 33.102652825836216 19.99243831922768 24-25 20.526899229387034 26.115539418133586 33.81247688270531 19.545084469774068 26-27 20.599667763179493 26.130655740934074 33.82134720060932 19.448329295277116 28-29 19.876088385105437 26.16545368689856 34.390425001627825 19.56803292636818 30-31 19.957059236149032 26.60762494022326 34.118811534475725 19.316504289151986 32-33 19.85244391139631 26.650649688889082 33.58175881079685 19.915147588917755 34-35 20.41223564326532 26.234321344857094 33.60025256559812 19.753190446279472 36-37 20.74317618001775 26.164423886078346 33.489545545571836 19.60285438833207 38-39 20.75896618816356 26.140758265832954 33.66651273442749 19.433762811575995 40-41 20.31418591366809 26.457450137991863 33.754002420602696 19.47436152773735 42-43 20.0843249218175 26.96531515139908 33.08770941008123 19.86265051670219 44-45 20.298646782414956 27.26843958670288 32.2394842759312 20.193429354950965 46-47 20.455022969462323 27.388867853914654 31.38187743346078 20.774231743162236 48-49 20.882622655177922 27.641597597781626 31.040727844153455 20.435051902886993 50-51 21.392623508735838 28.01021424303639 30.489690552427057 20.10747169580071 52-53 21.03648194068429 28.310983977185927 30.442252429904293 20.210281652225486 54-55 20.39018539715612 28.3344568198183 30.627135262666034 20.648222520359543 56-57 20.999439715830874 28.836578677247864 29.76088341055479 20.40309819636647 58-59 20.94122268888252 28.077514001632704 30.122879510451707 20.858383799033074 60-61 21.03270658837279 28.01021424303639 30.515242574593305 20.441836593997518 62-63 21.603605516172735 27.7563901941516 30.478528641245234 20.16147564843044 64-65 21.155214035118437 27.711687834173393 30.703517752910304 20.429580377797866 66-67 20.802957249880176 28.260481800613245 30.343381971543693 20.593178977962886 68-69 20.885084841468032 28.587897861946853 30.082226079039465 20.44479121754565 70-71 20.774286458413126 28.648303498930865 29.72630337199148 20.851106670664528 72-73 20.957867592303312 28.45014042676825 29.490934498695857 21.10105748223258 74-75 21.008522447478832 28.650820400471865 29.49141079991508 20.849246352134223 76-77 21.191326100706704 28.121614493851098 30.025650509617847 20.66140889582435 78-79 20.93471157402645 28.291997785126643 29.95911676453401 20.814173876312893 80-81 20.694073900606462 29.132642899820755 29.565768825876376 20.607514373696407 82-83 20.832767941170033 29.037611345779258 29.146987371692457 20.98263334135825 84-85 20.714648790050987 28.54475090540309 29.218461632740382 21.52213867180554 86-87 21.024171009233747 28.4308103766379 29.46055139841238 21.08446721571597 88-89 21.4608534265973 27.80284344215832 29.618733188739167 21.117569942505217 90-91 20.792923290411686 28.334963962011823 29.827317517329533 21.044795230246958 92-93 20.751734390756774 28.612481922885202 29.538840011446787 21.096943674911238 94-95 20.416394002333057 28.659574840614475 29.272385650597162 21.651645506455306 96-97 20.67393868827846 28.278264257152923 28.952903300642795 22.09489375392582 98-99 20.680070517401187 28.308020095927304 28.97839501782086 22.033514368850657 100-101 20.818980211982037 28.519184708049988 29.388443482310294 21.273391597657678 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1541.0 1 1091.5 2 1274.5 3 1647.5 4 1066.5 5 790.0 6 1244.0 7 2457.5 8 3655.0 9 4032.0 10 3543.0 11 2951.0 12 2899.5 13 3069.5 14 3297.0 15 3434.0 16 3354.5 17 3372.0 18 3535.0 19 3625.0 20 3736.5 21 4021.5 22 4563.0 23 5218.5 24 5793.0 25 6502.0 26 7691.5 27 9067.0 28 9993.0 29 11153.5 30 12781.0 31 13917.5 32 15364.5 33 17756.0 34 20203.5 35 21772.5 36 24260.0 37 26675.0 38 27606.5 39 28285.0 40 28272.0 41 28597.0 42 29211.0 43 30220.5 44 31694.5 45 31923.5 46 30814.0 47 31764.0 48 42331.5 49 42458.5 50 32666.0 51 29037.5 52 24945.5 53 22862.5 54 21513.0 55 19558.0 56 17254.0 57 15531.5 58 14356.0 59 12858.0 60 11094.5 61 9210.5 62 7197.5 63 5281.0 64 3832.0 65 2902.0 66 2096.0 67 1540.0 68 1166.5 69 861.5 70 667.5 71 538.0 72 473.5 73 434.0 74 358.0 75 296.5 76 215.0 77 118.0 78 75.5 79 56.0 80 31.0 81 15.0 82 9.0 83 5.0 84 3.0 85 2.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03884782813283112 2 0.0 3 0.0 4 0.0 5 0.016195714263828185 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003939498064174424 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0943050178262287E-4 22-23 4.377220071304915E-4 24-25 4.377220071304915E-4 26-27 0.001750888028521966 28-29 0.0027904777954568835 30-31 5.471525089131143E-4 32-33 0.0 34-35 0.0 36-37 1.0943050178262287E-4 38-39 0.0 40-41 0.0 42-43 0.010341182418457861 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.4715250891311436E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1886100356524575E-4 84-85 2.7357625445655715E-4 86-87 0.0 88-89 0.0 90-91 0.007003552114087864 92-93 0.0031187693008047517 94-95 0.0 96-97 0.0 98-99 5.471525089131143E-4 100-101 0.012310931450545072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 913822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.00672867952786 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88845189279365 42.605072173220925 2 12.29531286714923 13.280592520920726 3 3.7954560121338634 6.149404891871891 4 1.5511084812541451 3.3508117959842854 5 0.8210030318745578 2.2169843993759506 6 0.5157142886202418 1.6711224997001475 7 0.3594221216774112 1.3587849104796539 8 0.2489585921016441 1.0756351308856593 9 0.19941904411749936 0.9692973188266125 >10 1.1668678087972615 11.996043909822534 >50 0.10246989482115569 3.7912957454334872 >100 0.05011214347399091 4.964360757850198 >500 0.0030556184921078358 1.2667239081434687 >1k 0.002037078994738557 2.368203825490386 >5k 4.0741579894771143E-4 1.7304412325159473 >10k+ 2.0370789947385572E-4 1.2052249794781744 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10955 1.1988111470286336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9245 1.0116849889803485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6484 0.7095473735585267 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4066 0.4449444202481446 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3493 0.38224074272670167 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2384 0.2608823162497729 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2187 0.23932450739859623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2122 0.23221152478272575 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1847 0.20211813679250445 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1781 0.19489572367485133 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1329 0.1454331368691058 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.13503723919975663 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1083 0.11851323343058057 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 967 0.10581929522379632 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 951 0.10406840719527435 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 940 0.10286467167566549 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 932 0.10198922766140452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0943050178262287E-4 0.0 18-19 0.0 0.0 0.0 1.0943050178262287E-4 0.0 20-21 0.0 0.0 0.0 1.0943050178262287E-4 0.0 22-23 0.0 0.0 0.0 2.7357625445655715E-4 0.0 24-25 0.0 0.0 0.0 6.565830106957372E-4 0.0 26-27 0.0 0.0 0.0 9.301592651522945E-4 0.0 28-29 0.0 0.0 0.0 0.001367881272282786 0.0 30-31 0.0 0.0 0.0 0.0032281998025873748 0.0 32-33 0.0 0.0 0.0 0.005143233583783276 0.0 34-35 0.0 0.0 0.0 0.006894121612305241 0.0 36-37 0.0 0.0 0.0 0.00913744689884901 0.0 38-39 0.0 0.0 0.0 0.014007104228175728 0.0 40-41 0.0 0.0 0.0 0.020463503833350476 0.0 42-43 0.0 0.0 0.0 0.025278445911785886 0.0 44-45 0.0 0.0 0.0 0.027904777954568832 0.0 46-47 0.0 0.0 0.0 0.03140655401161276 0.0 48-49 0.0 0.0 0.0 0.03665921809717866 0.0 50-51 0.0 0.0 0.0 0.04574194974513636 0.0 52-53 0.0 0.0 0.0 0.061116935245594875 0.0 54-55 0.0 0.0 0.0 0.07205998542385716 0.0 56-57 0.0 0.0 0.0 0.10040248538555649 0.0 58-59 0.0 0.0 0.0 0.14937263493328024 0.0 60-61 0.0 1.0943050178262287E-4 0.0 0.1991087979934823 0.0 62-63 0.0 1.0943050178262287E-4 0.0 0.2305153520050951 0.0 64-65 0.0 1.0943050178262287E-4 0.0 0.27620258649934015 0.0 66-67 0.0 1.0943050178262287E-4 0.0 0.3476607041633929 0.0 68-69 0.0 1.0943050178262287E-4 0.0 0.46037412099949443 0.0 70-71 0.0 1.0943050178262287E-4 0.0 0.5761515918855094 0.0 72-73 0.0 1.0943050178262287E-4 0.0 0.7005740724123517 0.0 74-75 0.0 1.0943050178262287E-4 0.0 0.8398243859307393 0.0 76-77 0.0 1.0943050178262287E-4 0.0 0.9534679620319931 0.0 78-79 0.0 1.0943050178262287E-4 0.0 1.0996123971626859 0.0 80-81 0.0 1.0943050178262287E-4 0.0 1.280063294602231 0.0 82-83 0.0 1.0943050178262287E-4 0.0 1.4491334198563832 0.0 84-85 0.0 1.0943050178262287E-4 0.0 1.6598418510388238 0.0 86-87 0.0 1.0943050178262287E-4 0.0 1.9236787908367274 0.0 88-89 0.0 1.0943050178262287E-4 0.0 2.2245032402371576 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 70 2.0513652E-4 33.933186 2 AATACCG 70 2.0513652E-4 33.933186 5 TACCTGG 825 0.0 31.095135 2 GTACCTG 895 0.0 30.799791 1 TATCACG 65 0.0058395728 29.234743 2 GGACCGA 140 5.805341E-7 27.14209 6 TATACCG 95 0.0012253907 25.003399 5 GTATCAA 14550 0.0 23.224665 1 GTGTAGC 415 0.0 22.904705 1 GAGTACT 8155 0.0 22.36724 12-13 ACCTGGG 1095 0.0 22.126293 3 GGTATCA 11020 0.0 21.865992 1 AGTACTT 8270 0.0 21.539265 12-13 GTACATG 8545 0.0 21.35807 1 GACGAGT 135 3.7958456E-4 21.110518 6 GTACTTT 8710 0.0 21.022657 14-15 TACATGG 8670 0.0 20.821747 2 GGAACGC 140 4.8498245E-4 20.357683 8 CGGTTAC 70 4.8532736E-4 20.357124 28-29 ACATGGG 8595 0.0 20.119081 3 >>END_MODULE