FastQCFastQC Report
Thu 26 May 2016
SRR937531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301621
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88620.6808433484094064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.5371763362760742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.34825805668470317No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34720.2667443134368606No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27360.210199435934116No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19140.14704741241882238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18330.14082440280235184No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17550.13483187502352834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.13390994767294012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG550.002579435734.546318
GTATCAA121750.026.6474381
GTACATG77000.026.0094661
TACATGG78050.025.0965122
GGTATCA87650.024.965831
GAGTACT67300.024.24372312-13
ACATGGG78650.023.5751743
GTACTTT71850.022.51012814-15
TATCAAC143300.022.4943032
ATCAACG143600.022.4804153
TCAACGC143800.022.4500164
CAACGCA144200.022.3547695
AACGCAG145750.022.1617836
AGTACTT70600.022.13496412-13
CAATCGG550.002882743321.58521388-89
ATGGGCG1805.1272873E-621.1311555
ACGCAGA157400.020.2800437
CGCAGAG157650.020.2780158
GTACCTG9900.020.1815361
ATGGGAT21300.020.0894785
Produced by FastQC (version 0.11.5)