##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937531_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1301621 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87388110671232 33.0 31.0 34.0 30.0 34.0 2 32.01702953471095 33.0 31.0 34.0 30.0 34.0 3 32.0286811598768 34.0 31.0 34.0 30.0 34.0 4 35.609596034483154 37.0 35.0 37.0 35.0 37.0 5 35.60212458157943 37.0 35.0 37.0 33.0 37.0 6 35.6763773786686 37.0 35.0 37.0 35.0 37.0 7 35.62197828707435 37.0 35.0 37.0 33.0 37.0 8 35.61859635024327 37.0 35.0 37.0 33.0 37.0 9 37.26518548794157 39.0 37.0 39.0 34.0 39.0 10-11 37.14777957638975 39.0 37.0 39.0 34.0 39.0 12-13 37.0361645210088 39.0 37.0 39.0 33.0 39.0 14-15 38.19536754554513 40.0 38.0 41.0 33.5 41.0 16-17 38.12308728884983 40.0 38.0 41.0 33.0 41.0 18-19 38.09445837152289 40.0 38.0 41.0 33.5 41.0 20-21 38.01912768770633 40.0 38.0 41.0 33.0 41.0 22-23 37.94233306008431 40.0 38.0 41.0 33.0 41.0 24-25 37.9556852570756 40.0 38.0 41.0 33.0 41.0 26-27 37.85763328956739 40.0 38.0 41.0 33.0 41.0 28-29 37.73405737922175 40.0 38.0 41.0 33.0 41.0 30-31 37.473965924028576 40.0 37.0 41.0 32.5 41.0 32-33 37.30352076372462 40.0 37.0 41.0 32.5 41.0 34-35 37.30271945520239 40.0 37.0 41.0 32.5 41.0 36-37 37.162651032827526 40.0 36.5 41.0 32.0 41.0 38-39 36.99897281927689 40.0 36.0 41.0 31.0 41.0 40-41 36.764884709143445 39.5 36.0 41.0 31.0 41.0 42-43 36.47499540957007 39.0 36.0 41.0 30.0 41.0 44-45 36.17816899081991 39.0 35.0 41.0 30.0 41.0 46-47 35.74586842099198 39.0 35.0 41.0 28.0 41.0 48-49 35.354176446139085 39.0 35.0 40.5 26.5 41.0 50-51 34.42194348431686 38.0 34.0 39.5 24.5 40.5 52-53 34.49592431283761 38.0 34.5 40.0 24.0 40.5 54-55 34.77457762282569 39.0 35.0 40.0 24.0 41.0 56-57 34.55059806195506 38.0 34.0 40.0 23.5 41.0 58-59 34.31036031225679 38.0 34.0 40.0 23.0 41.0 60-61 33.97565381935294 38.0 34.0 40.0 22.0 41.0 62-63 33.61493360970667 37.5 33.5 40.0 20.5 41.0 64-65 33.23510299849188 37.0 33.0 40.0 19.5 41.0 66-67 32.78322453310142 36.0 32.5 39.0 17.5 41.0 68-69 32.28884713753082 36.0 32.0 39.0 13.5 40.0 70-71 31.829136899297108 35.0 31.0 38.5 9.0 40.0 72-73 31.375297033468268 35.0 31.0 38.0 7.5 40.0 74-75 30.862139209493392 35.0 30.5 37.0 4.0 39.0 76-77 30.391829495682693 34.5 30.0 36.5 2.0 39.0 78-79 29.835525471700286 34.0 29.5 36.0 2.0 38.5 80-81 29.29054540453788 34.0 29.0 35.5 2.0 37.5 82-83 28.799347505917623 34.0 29.0 35.0 2.0 37.0 84-85 28.28655576392821 34.0 27.5 35.0 2.0 36.5 86-87 27.77717361659039 33.5 26.5 35.0 2.0 36.0 88-89 27.320045159074724 33.0 26.0 35.0 2.0 36.0 90-91 26.779227209763825 33.0 25.0 35.0 2.0 35.0 92-93 26.16702480983328 32.5 24.5 35.0 2.0 35.0 94-95 25.43784481043253 32.0 21.5 34.0 2.0 35.0 96-97 24.6412703851582 31.5 15.0 34.0 2.0 35.0 98-99 23.63971770584525 31.0 2.0 34.0 2.0 35.0 100-101 20.905533177476393 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 8.004053108017217E-4 1101 2 0.1844278727331492 1101 3 0.22609412978071575 1101 4 0.13628905312569373 1101 5 -0.0409994282082522 1101 6 0.09765604844707809 1101 7 0.11563727435271431 1101 8 0.2707486980737457 1101 9 0.19512004707225117 1101 10-11 0.18947703423892648 1101 12-13 0.19691423243091322 1101 14-15 0.3416166485093086 1101 16-17 0.27074180754171806 1101 18-19 0.3516661355316657 1101 20-21 0.35316920332614643 1101 22-23 0.3384223621864919 1101 24-25 0.35965175026048257 1101 26-27 0.3548435115447006 1101 28-29 0.32809631575969433 1101 30-31 0.32495402763649395 1101 32-33 0.2848118319388746 1101 34-35 0.26839512675931587 1101 36-37 0.43167049072624053 1101 38-39 0.3270605820464425 1101 40-41 0.3852233530938278 1101 42-43 0.46201730891614545 1101 44-45 0.5080728073062772 1101 46-47 0.448164151458748 1101 48-49 0.6246201596176633 1101 50-51 0.502802775183234 1101 52-53 0.4721063081610666 1101 54-55 0.5994888249678354 1101 56-57 0.36134792835064644 1101 58-59 0.4895640610123593 1101 60-61 0.5246238027973789 1101 62-63 0.633776736034612 1101 64-65 0.571988475806414 1101 66-67 0.6266952527261651 1101 68-69 0.6535743591672869 1101 70-71 0.6572957060835058 1101 72-73 0.6965597919959734 1101 74-75 0.7604759875326579 1101 76-77 0.6539830722519646 1101 78-79 0.7153740801236452 1101 80-81 0.730452115930305 1101 82-83 0.7560820717599732 1101 84-85 0.8495675981897328 1101 86-87 0.8983427407389435 1101 88-89 0.8150202808283318 1101 90-91 1.022663965183579 1101 92-93 0.9981292961990818 1101 94-95 0.9857325276462845 1101 96-97 1.0691501902236347 1101 98-99 1.3377919936323224 1101 100-101 1.5287554795492362 1102 1 0.31502141864686095 1102 2 0.4395241366789726 1102 3 0.3710242570117295 1102 4 0.30881924335499633 1102 5 0.1438920487186266 1102 6 0.26545923837416296 1102 7 0.23399106084809773 1102 8 0.23551434868009835 1102 9 0.2810105577415243 1102 10-11 0.2072655387530915 1102 12-13 0.3738500465348693 1102 14-15 0.39293791000756784 1102 16-17 0.5578724906901016 1102 18-19 0.41793246507233306 1102 20-21 0.3842043759542406 1102 22-23 0.4937493360975367 1102 24-25 0.6584967106098674 1102 26-27 0.3563993139854631 1102 28-29 0.4864306068373452 1102 30-31 0.6083931385092356 1102 32-33 0.6156050145019663 1102 34-35 0.627847211875121 1102 36-37 0.6758729451056809 1102 38-39 0.6173688480184225 1102 40-41 0.6157662451411454 1102 42-43 0.4899823789166149 1102 44-45 0.5376436952842454 1102 46-47 0.7573474296685774 1102 48-49 0.8425793554529619 1102 50-51 0.719776558528693 1102 52-53 0.7186871609366108 1102 54-55 0.6761775410370845 1102 56-57 0.7466208003491701 1102 58-59 0.6716791435699179 1102 60-61 0.6777979803078367 1102 62-63 0.6086519100248253 1102 64-65 0.7091491682768378 1102 66-67 0.694176853393607 1102 68-69 0.6194818538358433 1102 70-71 0.6278546456689931 1102 72-73 0.6697706191891015 1102 74-75 0.6357446880225943 1102 76-77 0.5212003354869275 1102 78-79 0.587651425852318 1102 80-81 0.5304463694643857 1102 82-83 0.678116363263289 1102 84-85 0.8473722353749018 1102 86-87 0.8858207658275852 1102 88-89 0.7656602668665471 1102 90-91 0.6980269502810792 1102 92-93 0.7797795535130554 1102 94-95 0.9214019057935374 1102 96-97 1.083176887878647 1102 98-99 1.01247604442257 1102 100-101 1.1395633049588305 1103 1 0.15385475836515283 1103 2 0.2862845345898144 1103 3 0.23496377865036422 1103 4 0.22109905793569595 1103 5 0.27036180315298 1103 6 0.23791578870682173 1103 7 0.21932173803718058 1103 8 0.18357217089721445 1103 9 0.16135381330602172 1103 10-11 0.2695347542966431 1103 12-13 0.28325381877049693 1103 14-15 0.3696589765516407 1103 16-17 0.34876970556961595 1103 18-19 0.27839052225605343 1103 20-21 0.3246651148220536 1103 22-23 0.3243963881605225 1103 24-25 0.271109181717911 1103 26-27 0.13978819648939123 1103 28-29 0.22518145284482927 1103 30-31 0.33243839512086737 1103 32-33 0.3104491575762012 1103 34-35 0.3524451508093449 1103 36-37 0.36855360760935696 1103 38-39 0.402327537313397 1103 40-41 0.3679746758451472 1103 42-43 0.37496584186468596 1103 44-45 0.38390734314081953 1103 46-47 0.3501651134597026 1103 48-49 0.46957446457196284 1103 50-51 0.29884895122940947 1103 52-53 0.5288739849287438 1103 54-55 0.48810835358736426 1103 56-57 0.47922190622461613 1103 58-59 0.5875438589921558 1103 60-61 0.8087123068858872 1103 62-63 0.7770956393535116 1103 64-65 0.7626570664749934 1103 66-67 0.9481863542172633 1103 68-69 0.8644545900475222 1103 70-71 0.8447993135871066 1103 72-73 0.5843423797785618 1103 74-75 0.6067578538145249 1103 76-77 0.9327036109725064 1103 78-79 0.9156530604026223 1103 80-81 1.0858345113127044 1103 82-83 0.999064274742171 1103 84-85 0.9090288776510107 1103 86-87 0.9577559201521204 1103 88-89 1.158358424166476 1103 90-91 1.2179309204505344 1103 92-93 1.0134539715237558 1103 94-95 0.8238085257222849 1103 96-97 0.9773945384679799 1103 98-99 1.054136389976719 1103 100-101 1.0439069947007518 1104 1 0.12069394532941402 1104 2 0.15754462185899243 1104 3 0.1320017946423313 1104 4 0.06353875581598345 1104 5 0.18365049286008883 1104 6 0.2368732837416303 1104 7 0.1376082497062967 1104 8 0.2762021333168292 1104 9 0.25310015678630293 1104 10-11 0.3013231819008766 1104 12-13 0.4079114238146815 1104 14-15 0.39678034864174805 1104 16-17 0.37323209934412205 1104 18-19 0.3120783466545092 1104 20-21 0.2888671776824481 1104 22-23 0.39166247057156767 1104 24-25 0.24553650538912564 1104 26-27 0.30393252288499184 1104 28-29 0.3499410934316458 1104 30-31 0.36241026520643516 1104 32-33 0.3967651724311523 1104 34-35 0.4325372928912472 1104 36-37 0.4600514332629544 1104 38-39 0.3578020911847659 1104 40-41 0.3887702872831724 1104 42-43 0.4886347653232619 1104 44-45 0.5569275804362093 1104 46-47 0.47628887031683576 1104 48-49 0.450598068597273 1104 50-51 0.5398679776833433 1104 52-53 0.7094197495677435 1104 54-55 0.7303100177560538 1104 56-57 0.6669686336302405 1104 58-59 0.6418294256142758 1104 60-61 0.7226278285444394 1104 62-63 0.8401345560933962 1104 64-65 0.7421628364298911 1104 66-67 0.907409348391937 1104 68-69 0.799773211164883 1104 70-71 0.7679002490359643 1104 72-73 0.8357293100739582 1104 74-75 0.8667850239156003 1104 76-77 0.7574430216471058 1104 78-79 0.7589010687899176 1104 80-81 0.8308912510482358 1104 82-83 0.8973704256691342 1104 84-85 0.921181296011504 1104 86-87 0.9904682788565218 1104 88-89 0.9790977757756387 1104 90-91 1.194955794382743 1104 92-93 1.2123042627901945 1104 94-95 1.2708185594634145 1104 96-97 1.3323956828608239 1104 98-99 1.4584521538035702 1104 100-101 1.5225031569239746 1105 1 0.15908271483752756 1105 2 0.21234094148859484 1105 3 0.13355203149516015 1105 4 0.1300679703251575 1105 5 0.16886319965054497 1105 6 0.22721124118032066 1105 7 0.24967372172675084 1105 8 0.27467221316509693 1105 9 0.28240736090711493 1105 10-11 0.2574284049982083 1105 12-13 0.2992948932034025 1105 14-15 0.2763024925761428 1105 16-17 0.33024957860022397 1105 18-19 0.33314930527264863 1105 20-21 0.22746938139147943 1105 22-23 0.4016252728686567 1105 24-25 0.29764412730837364 1105 26-27 0.22817861908529835 1105 28-29 0.2813700504629111 1105 30-31 0.28635596446918754 1105 32-33 0.2691650428396244 1105 34-35 0.41699864453961055 1105 36-37 0.3038308766071083 1105 38-39 0.3553432261555045 1105 40-41 0.29178524944512674 1105 42-43 0.2369453046482235 1105 44-45 0.17303571317538058 1105 46-47 0.4508790262221183 1105 48-49 0.49700830705367594 1105 50-51 0.2953466845108039 1105 52-53 0.4082868758030287 1105 54-55 0.4919477687988305 1105 56-57 0.40247039991344735 1105 58-59 0.41721922510657805 1105 60-61 0.3973503695252205 1105 62-63 0.5998095686915619 1105 64-65 0.6150891270755352 1105 66-67 0.5126823304809491 1105 68-69 0.5789489916255164 1105 70-71 0.44075800709525836 1105 72-73 0.6520024797296635 1105 74-75 0.5996566844032074 1105 76-77 0.5761768703232377 1105 78-79 0.5414125976107904 1105 80-81 0.5835956397821889 1105 82-83 0.5584062218824606 1105 84-85 0.6240727616546913 1105 86-87 0.6133893996273372 1105 88-89 0.5082428090859601 1105 90-91 0.673332838251941 1105 92-93 0.7782658794626585 1105 94-95 0.99941863354681 1105 96-97 0.8448222479633358 1105 98-99 0.6988551428256571 1105 100-101 0.8345649646822686 1106 1 0.08406757403524168 1106 2 0.05557599275683245 1106 3 -0.014919201937260596 1106 4 0.0834850078178988 1106 5 -0.0016590695275624512 1106 6 -9.245313928687438E-4 1106 7 -0.005551409804482432 1106 8 0.06957150889055441 1106 9 0.12211434213251948 1106 10-11 -0.018369799919753405 1106 12-13 -0.10304166984883523 1106 14-15 -0.08169424294251115 1106 16-17 -0.07075014489475961 1106 18-19 -0.01722679895347312 1106 20-21 -0.155767526000389 1106 22-23 -0.1364797971234637 1106 24-25 -0.11883844187289583 1106 26-27 -0.14207379459298863 1106 28-29 -0.010630965621260202 1106 30-31 -0.14907915779099312 1106 32-33 -0.04854926529029058 1106 34-35 0.2490885823403275 1106 36-37 0.1375642970698152 1106 38-39 0.09064253565837532 1106 40-41 0.15443975499388785 1106 42-43 0.14932048272606835 1106 44-45 -0.00663394465684064 1106 46-47 0.12781927339272414 1106 48-49 0.20762212905141553 1106 50-51 0.2807785059325738 1106 52-53 0.3085441498462842 1106 54-55 0.32652051114494896 1106 56-57 0.3210021200138229 1106 58-59 0.35062433174279306 1106 60-61 0.24829407444367035 1106 62-63 0.38819281803690586 1106 64-65 0.26836882098735515 1106 66-67 0.5022473756545978 1106 68-69 0.21081413754649247 1106 70-71 0.2187995861780898 1106 72-73 0.4006754470111815 1106 74-75 0.45189560961194886 1106 76-77 0.569614181661187 1106 78-79 0.5761608584516509 1106 80-81 0.46679714687803653 1106 82-83 0.23298900069088901 1106 84-85 0.455777840831761 1106 86-87 0.4910476509101187 1106 88-89 0.7618933450177359 1106 90-91 0.7398519080087063 1106 92-93 0.5322469776181116 1106 94-95 0.6419940433530762 1106 96-97 1.101885085402305 1106 98-99 0.9576317851362788 1106 100-101 0.9790790190781742 1107 1 0.08515559504715853 1107 2 0.09144595015481727 1107 3 0.16533708247658652 1107 4 0.1809239565049907 1107 5 0.15731189342145768 1107 6 0.16307642776880726 1107 7 0.13096889506550013 1107 8 0.18421822140873445 1107 9 0.18162830712311262 1107 10-11 0.1562637353305547 1107 12-13 0.23922609761272895 1107 14-15 0.26235402440364197 1107 16-17 0.35419377328068435 1107 18-19 0.3037623701391112 1107 20-21 0.24357820472170033 1107 22-23 0.285804576384848 1107 24-25 0.1273204293641328 1107 26-27 0.08726164979243833 1107 28-29 0.22091301575127886 1107 30-31 0.33998852809700253 1107 32-33 0.18520213255339257 1107 34-35 0.14236167673483635 1107 36-37 0.13472603010464468 1107 38-39 0.1855704080450522 1107 40-41 -0.006092646966571635 1107 42-43 -0.007310282608749219 1107 44-45 0.044737230742008194 1107 46-47 0.2462987108040693 1107 48-49 0.2322068098052057 1107 50-51 0.05375916806787728 1107 52-53 0.08758848418135301 1107 54-55 0.2653212901276518 1107 56-57 0.24394983897945366 1107 58-59 0.18048350529502954 1107 60-61 0.26218735202012766 1107 62-63 0.3242270430768457 1107 64-65 0.33485631714958686 1107 66-67 0.3361930709683776 1107 68-69 0.3603004930234661 1107 70-71 0.28633756606706484 1107 72-73 0.2967178966383841 1107 74-75 0.22977068552690483 1107 76-77 0.08433776157526651 1107 78-79 0.21173152603266132 1107 80-81 0.3678219707889525 1107 82-83 0.3700673225165225 1107 84-85 0.45345669198913896 1107 86-87 0.34034497534207375 1107 88-89 0.38318807881675454 1107 90-91 0.4069197193944909 1107 92-93 0.29852919525368904 1107 94-95 0.5042887928393185 1107 96-97 0.4330507805816204 1107 98-99 0.582257252671571 1107 100-101 0.47507485524080906 1108 1 -0.1825131290665638 1108 2 -0.10877104812534455 1108 3 -0.17541137810776775 1108 4 -0.09609991330582801 1108 5 -0.20680485997112896 1108 6 -0.10496048395668822 1108 7 -0.013404684476476803 1108 8 -0.21694603430184145 1108 9 -0.23028433577893992 1108 10-11 -0.15623458274929192 1108 12-13 -0.07457983693549863 1108 14-15 -0.15037205624535233 1108 16-17 -0.03155125007049264 1108 18-19 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-0.5640331354419814 2316 60-61 -0.5006630796323606 2316 62-63 -0.4444133278697322 2316 64-65 -0.38731201206550736 2316 66-67 -0.9107746847437745 2316 68-69 -0.6058979145907024 2316 70-71 -0.7999502225909971 2316 72-73 -0.6794176696957734 2316 74-75 -0.8980411566987705 2316 76-77 -0.828817724834547 2316 78-79 -0.2875256656332432 2316 80-81 -0.5091067379142586 2316 82-83 -0.4581898996548581 2316 84-85 -0.5228573313780522 2316 86-87 -0.6064122925875175 2316 88-89 -0.5146780631557277 2316 90-91 -0.6137202854863837 2316 92-93 -0.6070655089957278 2316 94-95 -0.35867755962094705 2316 96-97 -0.18526470704840747 2316 98-99 -0.39103367805049416 2316 100-101 -0.8645654852002984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5531.0 3 1072.0 4 910.0 5 1076.0 6 1095.0 7 1386.0 8 1535.0 9 1958.0 10 2471.0 11 3410.0 12 4552.0 13 6181.0 14 7052.0 15 9310.0 16 12304.0 17 17366.0 18 14829.0 19 9372.0 20 8598.0 21 8834.0 22 9374.0 23 10530.0 24 11833.0 25 13398.0 26 15352.0 27 18121.0 28 21275.0 29 26101.0 30 31766.0 31 39958.0 32 51459.0 33 67570.0 34 92694.0 35 130704.0 36 186392.0 37 250576.0 38 185149.0 39 20525.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38315659977847 19.00513281416808 14.863038881895942 21.74867170415751 2 14.958266268373361 20.27404880762418 41.277677057273024 23.49000786672944 3 19.55524290822628 25.301858342508577 30.20301615638462 24.939882592880526 4 12.088488701979754 16.605231161362205 38.05436425809693 33.251915878561114 5 12.929455452220395 38.09330062979736 34.21017955350889 14.767064364473356 6 30.56946833096478 36.48124739215653 17.778525380956854 15.170758895921832 7 27.63261318591136 33.905001512763796 20.671821098166436 17.790564203158404 8 26.329140625842047 34.244550481091245 19.858544238992025 19.567764654074686 9 26.586961877194064 16.96264704070949 21.13036583112644 35.320025250970005 10-11 24.907456881219904 26.921001128687287 26.848360169811237 21.323181820281572 12-13 26.309320810639157 24.49015569968629 27.80954213899421 21.390981350680345 14-15 22.951259487244233 25.442365977639398 27.28634983883521 24.320024696281163 16-17 21.249581368491967 29.209508228274466 27.63857184101626 21.902338562217302 18-19 21.0535898291606 27.15666315233972 30.983930711175827 20.80581630732385 20-21 22.200762690075944 25.856371325685906 30.9725838012264 20.970282183011747 22-23 21.596389773591078 25.942517343904857 30.92055000144332 21.540542881060745 24-25 21.56931574338693 26.121609268400643 31.200254034236885 21.108820953975542 26-27 21.247455915362423 26.224278188149874 31.373990448713407 21.154275447774292 28-29 20.82371785124915 26.663181675928964 31.403025705912718 21.110074766909165 30-31 21.062656799834002 26.32123769902054 31.19547253892832 21.420632962217134 32-33 20.865511691229433 26.418917852458645 31.16593542346854 21.549635032843387 34-35 21.64502297821362 26.338868789742225 30.809630754851064 21.206477477193094 36-37 21.535329355144736 26.14239503374276 31.057080784425413 21.26519482668709 38-39 21.249364550193896 26.454992451568238 31.119357580930618 21.17628541730725 40-41 21.323772493367976 26.483300922075586 31.037105085212808 21.15582149934363 42-43 21.08507212734781 26.573016647081936 30.54031907003275 21.8015921555375 44-45 21.262844535318045 26.716979882924967 30.22534326510628 21.794832316650705 46-47 21.618077210140562 27.03348020707311 29.745107918902907 21.603334663883416 48-49 21.730149944070256 27.175582450762192 29.3744336776368 21.719833927530754 50-51 21.48897094911466 27.41563025320066 29.463423384444116 21.63197541324056 52-53 21.802083473714003 27.628224503822523 28.977996653966624 21.591695368496847 54-55 21.328658678338677 27.33460758608904 29.213309295476275 22.123424440096006 56-57 21.619846223706794 27.619546059771103 28.914714959708764 21.84589275681334 58-59 21.91228206551061 27.156678860402632 29.13281833847584 21.79822073561092 60-61 21.732921804840526 27.170461992167823 29.688579237769297 21.408036965222355 62-63 21.607841401723675 27.220200768515863 29.575276648393412 21.59668118136705 64-65 21.33453344549431 27.51721473413462 29.384340922157154 21.76391089821392 66-67 21.39464526836574 27.517796322869298 28.947928415279726 22.139629993485233 68-69 21.288255822366448 27.439244035239767 29.223784534341533 22.04871560805225 70-71 21.57451983206148 27.440092050627847 29.0018741076823 21.983514009628372 72-73 21.5723729552529 27.22399257158755 29.260802925803226 21.942831547356324 74-75 21.31710335623571 27.835852543287338 28.826668247025477 22.02037585345148 76-77 21.48008035590585 27.462439001862656 28.847038992626345 22.210441649605148 78-79 21.48672036428894 27.524604782139008 28.900694548943434 22.087980304628623 80-81 21.487768685618587 27.786487676327344 28.692676296556268 22.033067341497805 82-83 21.469782942067216 27.589004142591346 28.836437871712434 22.10477504362901 84-85 21.47336430419269 27.445496687493048 28.670067997107886 22.41107101120638 86-87 21.48933959759434 27.515651032948934 28.774121060306978 22.220888309149746 88-89 21.469907251527562 27.394873456420765 28.529804148072785 22.60541514397889 90-91 21.34073645913747 27.437578457458066 28.771249416018673 22.450435667385786 92-93 21.69657183975496 27.490889921001088 28.811014441447302 22.001523797796647 94-95 21.388693272993848 27.332902337000654 28.693080584769664 22.585323805235834 96-97 21.235716168556426 27.306702890533757 28.783235943209085 22.674344997700736 98-99 21.421133283100655 27.45540438717543 28.285167172456894 22.838295157267023 100-101 21.658057481603688 26.68294627268327 28.94567556774068 22.713320677972362 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1777.0 1 1053.0 2 403.0 3 464.5 4 378.0 5 373.0 6 640.5 7 1436.5 8 2486.0 9 2949.0 10 2648.0 11 2259.0 12 2258.5 13 2458.0 14 2662.5 15 2802.5 16 2871.5 17 2931.5 18 3108.0 19 3289.5 20 3543.5 21 3942.0 22 4564.5 23 5449.5 24 6402.0 25 7704.5 26 9528.5 27 11472.5 28 12892.5 29 14752.0 30 17321.5 31 19418.5 32 21918.0 33 24444.0 34 27097.5 35 29680.0 36 32788.0 37 36073.0 38 37580.5 39 37880.5 40 38574.0 41 40438.5 42 41650.0 43 42868.0 44 44568.0 45 45870.5 46 46042.0 47 48979.5 48 58679.5 49 58297.5 50 48772.0 51 43804.0 52 40773.5 53 39582.5 54 38024.0 55 34629.5 56 30740.0 57 26977.5 58 24169.5 59 21537.5 60 18497.5 61 15442.5 62 12237.0 63 9304.5 64 6850.0 65 5278.0 66 3997.0 67 3047.0 68 2229.5 69 1555.5 70 1217.0 71 934.5 72 727.0 73 623.5 74 482.5 75 381.5 76 288.5 77 196.0 78 135.0 79 105.5 80 83.0 81 55.5 82 40.5 83 31.0 84 24.5 85 17.5 86 9.5 87 5.0 88 6.5 89 4.5 90 4.0 91 3.5 92 2.5 93 5.5 94 5.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32897440960156604 2 0.2881022970588213 3 0.28764133338352715 4 0.29063759727293886 5 0.2997800434996055 6 0.20397642631764545 7 0.2054361446227435 8 0.20735682660313562 9 0.20443738999293956 10-11 0.21258108158980224 12-13 0.20328498080470428 14-15 0.2028240171294102 16-17 0.21096770872627285 18-19 0.20378435811960624 20-21 0.19475715281176317 22-23 0.19464191189293964 24-25 0.19848327585372394 26-27 0.19429618913646907 28-29 0.20877813126862582 30-31 0.21446334993058658 32-33 0.21553893183960615 34-35 0.21373349077803755 36-37 0.1884957295556848 38-39 0.18012155612117506 40-41 0.1868823566921554 42-43 0.1970235575486259 44-45 0.2016716079411749 46-47 0.20432214907411603 48-49 0.20512883550588076 50-51 0.20824034031411603 52-53 0.2357445062733315 54-55 0.19660100751293963 56-57 0.1790843878517633 58-59 0.17823928778039075 60-61 0.1719010372450967 62-63 0.1815812744262731 64-65 0.17654908763764568 66-67 0.17405220106313588 68-69 0.18492326107215543 70-71 0.1794685242478417 72-73 0.1755887466474496 74-75 0.1796221788062731 76-77 0.1844622973968613 78-79 0.1868823566921554 80-81 0.17923804241019467 82-83 0.17773991046548882 84-85 0.17113276445293982 86-87 0.16794443236548887 88-89 0.17259248275803785 90-91 0.18054410615686134 92-93 0.17178579632627317 94-95 0.17716370587137115 96-97 0.1762033648811751 98-99 0.17931486968941038 100-101 0.15672764959999877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1301621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.58863820719912 #Duplication Level Percentage of deduplicated Percentage of total 1 77.69344312969999 43.965661443381926 2 13.05643865562282 14.77692166715058 3 4.079769189685527 6.926057479319765 4 1.7504089802079208 3.962130419824735 5 0.9069895873398564 2.5662652807835973 6 0.5483475861676214 1.8618145899258278 7 0.3574249472479514 1.4158333718229084 8 0.24258646833916828 1.0982110312645885 9 0.1917310872805396 0.9764821018072235 >10 1.0434661121974986 11.020098934420265 >50 0.0839649266368524 3.2510052066504502 >100 0.04081861319559251 4.176251109358713 >500 0.0027121861051412786 0.9808837665640767 >1k 0.0016273116630847672 1.8057562127225233 >5k 2.7121861051412785E-4 1.2166273850028848 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8862 0.6808433484094064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6992 0.5371763362760742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4533 0.34825805668470317 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3472 0.2667443134368606 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2736 0.210199435934116 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1914 0.14704741241882238 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1833 0.14082440280235184 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1755 0.13483187502352834 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1743 0.13390994767294012 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 7.682727921568568E-5 0.0 0.0 0.0 3 0.0 7.682727921568568E-5 0.0 0.0 0.0 4 0.0 7.682727921568568E-5 0.0 0.0 0.0 5 0.0 7.682727921568568E-5 0.0 0.0 0.0 6 0.0 7.682727921568568E-5 0.0 0.0 0.0 7 0.0 7.682727921568568E-5 0.0 0.0 0.0 8 0.0 7.682727921568568E-5 0.0 0.0 0.0 9 0.0 7.682727921568568E-5 0.0 0.0 0.0 10-11 0.0 7.682727921568568E-5 0.0 0.0 0.0 12-13 0.0 7.682727921568568E-5 0.0 0.0 0.0 14-15 0.0 7.682727921568568E-5 0.0 0.0 0.0 16-17 0.0 7.682727921568568E-5 0.0 3.841363960784284E-5 0.0 18-19 0.0 7.682727921568568E-5 0.0 7.682727921568568E-5 0.0 20-21 0.0 7.682727921568568E-5 0.0 7.682727921568568E-5 0.0 22-23 0.0 7.682727921568568E-5 0.0 1.1524091882352851E-4 0.0 24-25 0.0 7.682727921568568E-5 0.0 2.3048183764705701E-4 0.0 26-27 0.0 7.682727921568568E-5 0.0 3.841363960784284E-4 0.0 28-29 0.0 7.682727921568568E-5 0.0 9.603409901960709E-4 0.0 30-31 0.0 7.682727921568568E-5 0.0 0.002765782051764684 0.0 32-33 0.0 7.682727921568568E-5 0.0 0.005185841347058783 0.0 34-35 0.0 7.682727921568568E-5 0.0 0.00799003703843131 0.0 36-37 0.0 7.682727921568568E-5 0.0 0.012753328349803823 0.0 38-39 0.0 7.682727921568568E-5 0.0 0.019283647083137102 0.0 40-41 0.0 7.682727921568568E-5 0.0 0.02827243875137233 0.0 42-43 0.0 7.682727921568568E-5 0.0 0.041179421659607515 0.0 44-45 0.0 7.682727921568568E-5 0.0 0.053855922730195654 0.0 46-47 0.0 7.682727921568568E-5 0.0 0.07786444748509744 0.0 48-49 0.0 7.682727921568568E-5 0.0 0.10325586326588154 0.0 50-51 0.0 7.682727921568568E-5 0.0 0.12288523310548924 0.0 52-53 0.0 7.682727921568568E-5 0.0 0.15154180825294 0.0 54-55 0.0 7.682727921568568E-5 0.0 0.18868779775372402 0.0 56-57 0.0 7.682727921568568E-5 0.0 0.241237656737253 0.0 58-59 0.0 7.682727921568568E-5 0.0 0.3041207847752917 0.0 60-61 0.0 7.682727921568568E-5 0.0 0.3811017185494088 0.0 62-63 0.0 7.682727921568568E-5 0.0 0.46461297105685906 0.0 64-65 0.0 7.682727921568568E-5 0.0 0.5604165882388191 0.0 66-67 0.0 7.682727921568568E-5 0.0 0.6665534744752889 0.0 68-69 0.0 7.682727921568568E-5 0.0 0.7938178624960722 0.0 70-71 0.0 7.682727921568568E-5 0.0 0.9304167649415613 0.0 72-73 0.0 7.682727921568568E-5 0.0 1.0734307452015601 0.0 74-75 0.0 7.682727921568568E-5 0.0 1.2238585579058727 0.0 76-77 0.0 7.682727921568568E-5 0.0 1.4021746729654794 0.0 78-79 0.0 7.682727921568568E-5 0.0 1.614870995474105 0.0 80-81 0.0 7.682727921568568E-5 0.0 1.8731643081972402 0.0 82-83 0.0 7.682727921568568E-5 0.0 2.144249362909787 0.0 84-85 0.0 7.682727921568568E-5 0.0 2.4478707703701765 0.0 86-87 0.0 7.682727921568568E-5 0.0 2.7701996203195858 0.0 88-89 0.0 7.682727921568568E-5 0.0 3.156410352936838 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 55 0.0025794357 34.54631 8 GTATCAA 12175 0.0 26.647438 1 GTACATG 7700 0.0 26.009466 1 TACATGG 7805 0.0 25.096512 2 GGTATCA 8765 0.0 24.96583 1 GAGTACT 6730 0.0 24.243723 12-13 ACATGGG 7865 0.0 23.575174 3 GTACTTT 7185 0.0 22.510128 14-15 TATCAAC 14330 0.0 22.494303 2 ATCAACG 14360 0.0 22.480415 3 TCAACGC 14380 0.0 22.450016 4 CAACGCA 14420 0.0 22.354769 5 AACGCAG 14575 0.0 22.161783 6 AGTACTT 7060 0.0 22.134964 12-13 CAATCGG 55 0.0028827433 21.585213 88-89 ATGGGCG 180 5.1272873E-6 21.131155 5 ACGCAGA 15740 0.0 20.280043 7 CGCAGAG 15765 0.0 20.278015 8 GTACCTG 990 0.0 20.181536 1 ATGGGAT 2130 0.0 20.089478 5 >>END_MODULE