FastQCFastQC Report
Thu 26 May 2016
SRR937531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301621
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94930.729321361594504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84870.6520331187035243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.4545870111192121No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53270.40925891638195755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.1588019861388223No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17450.1340636022313715No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16860.12953079275764603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.12031151925176377No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13960.1072508817850972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73950.022.4291
GAGTACT79950.022.18934412-13
CGCGGAA1205.9426384E-921.7689382-83
ATTCGCG1205.9426384E-921.7689380-81
TACATGG76200.021.7633822
AGTACTT81950.021.41597612-13
GTATCAA149300.021.1045651
GTACTTT85400.020.9393314-15
ACATGGG74700.020.8645573
GGTATCA115200.020.7922131
CGATCGT1401.5788828E-920.35614824-25
CCCGCGA1401.5788828E-920.35614820-21
ACACGCT2601.4913894E-820.0951719
CCTATAC3353.45608E-1119.8581663
CGCTAGA1200.00474931719.8021091
TCGCGGA1201.4162106E-719.78993882-83
CGCGTAA857.964133E-519.55711464-65
AGAGTAC134300.019.04504810-11
TACTTTT92600.018.87524214-15
ACTTTTT95700.018.71084416-17
Produced by FastQC (version 0.11.5)