##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937531_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1301621 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00979010019046 31.0 31.0 34.0 30.0 34.0 2 32.311795829968936 33.0 31.0 34.0 31.0 34.0 3 32.39268650398234 34.0 31.0 34.0 31.0 34.0 4 36.06259963537773 37.0 35.0 37.0 35.0 37.0 5 35.97748576582584 37.0 35.0 37.0 35.0 37.0 6 36.03434486690058 37.0 35.0 37.0 35.0 37.0 7 35.973955552345885 37.0 35.0 37.0 35.0 37.0 8 35.95313920104239 37.0 35.0 37.0 35.0 37.0 9 37.618668567885734 39.0 37.0 39.0 35.0 39.0 10-11 37.529622678183586 39.0 37.0 39.0 35.0 39.0 12-13 37.40837770748935 39.0 37.0 39.0 34.0 39.0 14-15 38.53770721277546 40.0 38.0 41.0 34.0 41.0 16-17 38.49893939941043 40.0 38.0 41.0 34.0 41.0 18-19 38.51387385421717 40.0 38.0 41.0 34.0 41.0 20-21 38.43214076908716 40.0 38.0 41.0 34.0 41.0 22-23 38.36208082076119 40.0 38.0 41.0 34.0 41.0 24-25 38.37083375268223 40.0 38.0 41.0 34.0 41.0 26-27 38.20407322868945 40.0 38.0 41.0 34.0 41.0 28-29 38.034642188471146 40.0 37.0 41.0 33.0 41.0 30-31 38.066397207789365 40.0 37.0 41.0 34.0 41.0 32-33 37.95046561172569 40.0 37.0 41.0 33.0 41.0 34-35 37.777749437048115 40.0 37.0 41.0 33.0 41.0 36-37 37.590370391995826 40.0 36.5 41.0 33.0 41.0 38-39 37.44124364926503 40.0 36.0 41.0 33.0 41.0 40-41 37.235408386926764 40.0 36.0 41.0 32.5 41.0 42-43 36.96794535429284 39.0 36.0 41.0 31.5 41.0 44-45 36.59433583201255 39.0 35.5 41.0 31.0 41.0 46-47 36.11184976271895 39.0 35.0 41.0 30.0 41.0 48-49 35.6869069414215 39.0 35.0 40.0 28.5 41.0 50-51 35.36143931297974 39.0 35.0 40.0 27.0 41.0 52-53 35.11047839578495 38.5 35.0 40.0 26.5 41.0 54-55 34.868842773741356 38.0 34.0 40.0 26.0 41.0 56-57 34.57320487300066 38.0 34.0 40.0 25.5 41.0 58-59 34.2982527171888 38.0 34.0 40.0 25.0 41.0 60-61 33.92226001270723 37.5 33.5 40.0 23.5 41.0 62-63 33.53426035689344 37.0 33.0 40.0 22.5 41.0 64-65 33.09271631296668 36.0 32.5 39.0 21.5 40.5 66-67 32.6413687240756 36.0 32.0 39.0 20.0 40.0 68-69 32.18533812837992 35.0 31.0 38.5 18.5 40.0 70-71 31.76056816846071 35.0 31.0 38.0 18.0 40.0 72-73 31.242484179342526 35.0 30.5 37.0 14.5 39.0 74-75 30.74128375310478 34.0 30.0 36.5 10.0 39.0 76-77 29.575381389820848 33.5 28.5 35.5 8.0 37.5 78-79 30.179648684217604 34.0 30.0 36.0 7.5 38.0 80-81 29.97210555146237 34.0 30.0 35.5 7.0 37.0 82-83 29.577439592631038 34.0 29.5 35.0 2.0 37.0 84-85 29.158102473761563 34.0 29.0 35.0 2.0 36.0 86-87 28.74477094330838 34.0 29.0 35.0 2.0 36.0 88-89 28.249505040253652 33.5 28.0 35.0 2.0 35.5 90-91 27.620244679518848 33.0 26.5 35.0 2.0 35.0 92-93 27.113604113639838 33.0 26.0 35.0 2.0 35.0 94-95 26.53914964494273 32.0 25.0 34.0 2.0 35.0 96-97 25.838443371764896 32.0 24.5 34.0 2.0 35.0 98-99 24.797147556777283 31.5 18.5 34.0 2.0 35.0 100-101 21.99032398831918 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27592776601366964 1101 2 0.2831140414140805 1101 3 0.21866408301278994 1101 4 0.09323783319762668 1101 5 0.025402927654738505 1101 6 0.026128926195546853 1101 7 0.10574600575104398 1101 8 0.14968674271486293 1101 9 0.22986728218126729 1101 10-11 0.18885882197405124 1101 12-13 0.12218352487247586 1101 14-15 0.2367297236002628 1101 16-17 0.14444583298882208 1101 18-19 0.32080832061485864 1101 20-21 0.2919768966042966 1101 22-23 0.176687713703096 1101 24-25 0.16923855099496166 1101 26-27 0.02882264579866245 1101 28-29 -0.04236545762184818 1101 30-31 0.11794443061037185 1101 32-33 0.20630340047709694 1101 34-35 0.19739254843263154 1101 36-37 0.10758296277215607 1101 38-39 0.15616918552454706 1101 40-41 0.22055916285628996 1101 42-43 0.28603927990836553 1101 44-45 0.31343268008590286 1101 46-47 0.15252043455567588 1101 48-49 0.13734870344745076 1101 50-51 0.2346755205596125 1101 52-53 0.38786510808011343 1101 54-55 0.1588511717505483 1101 56-57 0.3881678205222485 1101 58-59 0.0929091571218521 1101 60-61 0.20553458565947835 1101 62-63 0.05945002708860159 1101 64-65 0.0292971882784272 1101 66-67 0.05998857739997021 1101 68-69 0.24888879604263892 1101 70-71 0.05232950122681501 1101 72-73 -0.03404417724009434 1101 74-75 -0.13174656265735152 1101 76-77 -0.01644114747953651 1101 78-79 -0.001473048365639329 1101 80-81 -0.022274369826710938 1101 82-83 0.22293318902767822 1101 84-85 0.09708141665602454 1101 86-87 -0.08510086426017338 1101 88-89 0.0012405077186663505 1101 90-91 -0.08817216069915901 1101 92-93 -0.20391704215778717 1101 94-95 -0.22547936763557175 1101 96-97 -0.4152280147266545 1101 98-99 -0.2704511715195146 1101 100-101 0.09735012815593436 1102 1 0.2657288531598958 1102 2 0.1522648840162475 1102 3 0.15639793225106757 1102 4 0.14191039956930496 1102 5 0.11206389042189357 1102 6 0.07616787446458062 1102 7 0.07795439388863912 1102 8 0.023862550518991554 1102 9 0.24273510920838248 1102 10-11 0.1570159516356071 1102 12-13 0.22855699407779895 1102 14-15 0.24339775239218397 1102 16-17 0.2923832773864419 1102 18-19 0.18359335596626636 1102 20-21 0.25306374485928274 1102 22-23 0.27304636979626906 1102 24-25 0.1982358565409399 1102 26-27 0.2504042434864502 1102 28-29 0.05499138088543276 1102 30-31 0.11942042102441519 1102 32-33 0.18522664046228243 1102 34-35 0.25933306612536455 1102 36-37 0.16360887720514938 1102 38-39 0.14319494246181108 1102 40-41 0.15143237027816525 1102 42-43 0.22588442329333702 1102 44-45 0.13674162286387315 1102 46-47 0.08789397612390815 1102 48-49 0.053991958586280475 1102 50-51 0.18377460240206034 1102 52-53 0.3317359700925664 1102 54-55 0.1810431718186507 1102 56-57 0.18524308219927121 1102 58-59 0.15210520525594973 1102 60-61 0.09312732723451944 1102 62-63 0.07213162605339107 1102 64-65 -0.1893154020155734 1102 66-67 -0.1666073438862199 1102 68-69 -0.08922793809179552 1102 70-71 0.05960090894069836 1102 72-73 -0.07039695916809663 1102 74-75 -0.14977024661023464 1102 76-77 -0.08184976377310704 1102 78-79 0.00606014279587086 1102 80-81 0.1776994518285271 1102 82-83 0.21296519897119381 1102 84-85 0.1952523378357931 1102 86-87 0.06435194448467385 1102 88-89 0.05454323580015341 1102 90-91 0.19833492448049483 1102 92-93 0.060350155737726396 1102 94-95 -0.06298101708412318 1102 96-97 0.14059996588008516 1102 98-99 0.0010672150431219052 1102 100-101 0.029436478383875198 1103 1 0.24191141199731803 1103 2 0.1774670957671347 1103 3 0.15857750292621375 1103 4 0.13849035845014868 1103 5 0.07790894016075356 1103 6 0.1371245971912174 1103 7 -0.015273715268676824 1103 8 0.08294311597123993 1103 9 0.12252721484119888 1103 10-11 0.07615078926602337 1103 12-13 0.09522825791720635 1103 14-15 0.1063882132587537 1103 16-17 0.11068921923220643 1103 18-19 0.1305437703503074 1103 20-21 0.06337660800400613 1103 22-23 -0.04847823146284469 1103 24-25 0.011172172928588964 1103 26-27 0.053565310541323186 1103 28-29 -0.006617501873890319 1103 30-31 0.07503918770512996 1103 32-33 0.09071898489268193 1103 34-35 0.15687306791315336 1103 36-37 0.0854569406461394 1103 38-39 0.08791521727058438 1103 40-41 0.10266594496306425 1103 42-43 0.17522638909547084 1103 44-45 0.307660674313901 1103 46-47 0.33308801512325203 1103 48-49 0.06192782802657604 1103 50-51 0.16076498664907746 1103 52-53 0.1816698218848245 1103 54-55 -0.1261031332037632 1103 56-57 0.027321259675680665 1103 58-59 -0.03533519112249195 1103 60-61 0.17618393630882423 1103 62-63 0.05198489962347708 1103 64-65 -0.06294908396784393 1103 66-67 -0.09369107627968276 1103 68-69 0.10085608800993029 1103 70-71 -0.13587636697905126 1103 72-73 -0.11314951634543391 1103 74-75 0.11088931997853635 1103 76-77 0.06975413871574787 1103 78-79 0.01577562888303774 1103 80-81 -0.006834254386596683 1103 82-83 0.0759105820050685 1103 84-85 0.19914971872432474 1103 86-87 0.06557734641803492 1103 88-89 0.02373209021024536 1103 90-91 0.15261668008968599 1103 92-93 0.3349862767455285 1103 94-95 0.16731524515904184 1103 96-97 -0.0477725072711479 1103 98-99 -0.08712264819099147 1103 100-101 -0.026574835769029193 1104 1 0.2611685164619253 1104 2 0.2652743652458085 1104 3 0.1823708329617233 1104 4 0.11231432868116542 1104 5 0.10708933338889892 1104 6 0.09863667238151663 1104 7 0.1304569611044144 1104 8 0.163817875094999 1104 9 0.11850869782666251 1104 10-11 0.19844902542440934 1104 12-13 0.14999432187030948 1104 14-15 0.20593680565555417 1104 16-17 0.24282273665281906 1104 18-19 0.1753598184295697 1104 20-21 0.10221892543643207 1104 22-23 0.07341466813328879 1104 24-25 0.04292014070838235 1104 26-27 0.23784962202290671 1104 28-29 0.16248728759474318 1104 30-31 0.21886278877716592 1104 32-33 0.10721374652672466 1104 34-35 0.14630094532283522 1104 36-37 0.2732850306913974 1104 38-39 0.24255443819746603 1104 40-41 0.19942029919326387 1104 42-43 0.3079129066711346 1104 44-45 0.18315442445233288 1104 46-47 0.23010151895881847 1104 48-49 0.2766609629984842 1104 50-51 0.2403240631609691 1104 52-53 0.3762318147526713 1104 54-55 0.2804711452750084 1104 56-57 0.3727278144625714 1104 58-59 0.4374802367696802 1104 60-61 0.4314377331089574 1104 62-63 0.36400058149703796 1104 64-65 0.3577279536051563 1104 66-67 0.5666937350431596 1104 68-69 0.5402359009201625 1104 70-71 0.3727491064673387 1104 72-73 0.37103069230322205 1104 74-75 0.44185895296067557 1104 76-77 0.4392387189450737 1104 78-79 0.5288245129154348 1104 80-81 0.5133388073441694 1104 82-83 0.48396577865606005 1104 84-85 0.4166441628816493 1104 86-87 0.5655004136861876 1104 88-89 0.603175693792565 1104 90-91 0.6409571628019677 1104 92-93 0.4954858226799814 1104 94-95 0.4933561627406071 1104 96-97 0.4245776326401085 1104 98-99 0.4685215711485107 1104 100-101 0.42798449915414594 1105 1 0.23065754780152403 1105 2 0.13467244306820447 1105 3 0.15552194967283128 1105 4 0.058068011173944 1105 5 -0.010781998766304923 1105 6 0.03370123051322338 1105 7 0.0749524593225317 1105 8 -0.032208178171764246 1105 9 0.032797977900727915 1105 10-11 0.1072319353056912 1105 12-13 0.09063962383272184 1105 14-15 0.048303694076594184 1105 16-17 0.030005474132380527 1105 18-19 0.04700470571360427 1105 20-21 -0.15349523744473714 1105 22-23 0.028345876146197213 1105 24-25 0.0828246812371205 1105 26-27 -0.06952737214293592 1105 28-29 -0.04451123545364766 1105 30-31 0.14776231652270866 1105 32-33 0.10063784714083113 1105 34-35 0.24730179681029796 1105 36-37 0.11201940179698511 1105 38-39 0.14539870379044117 1105 40-41 0.08852775386252887 1105 42-43 0.17733547337431332 1105 44-45 0.025841663713784158 1105 46-47 -0.05396060951311199 1105 48-49 0.009257078227555837 1105 50-51 -0.014656158893316729 1105 52-53 0.05810075534192549 1105 54-55 -0.09099742206741723 1105 56-57 0.0543743597345312 1105 58-59 0.029131777773315548 1105 60-61 -0.056314924116009024 1105 62-63 -0.08743760512129484 1105 64-65 0.03053238032408956 1105 66-67 0.07413726640506724 1105 68-69 0.10209064158653902 1105 70-71 -0.0635039786781384 1105 72-73 0.022013712257486162 1105 74-75 -0.15143044066924105 1105 76-77 -0.0917025284205586 1105 78-79 0.025948429917360016 1105 80-81 -0.29806905659778593 1105 82-83 -0.08386316052553155 1105 84-85 0.16790298205321008 1105 86-87 -0.10670122376736302 1105 88-89 0.015718100371117316 1105 90-91 0.43898901866368334 1105 92-93 0.4979332595648813 1105 94-95 0.3094221746048369 1105 96-97 0.2749117516734856 1105 98-99 0.22569744811347192 1105 100-101 0.26310467425508577 1106 1 -0.004177491602828098 1106 2 -0.04080156211171726 1106 3 -0.0668392692072004 1106 4 -0.014125151976458028 1106 5 -0.045562311226539975 1106 6 -0.11262637148029597 1106 7 -0.1555977091159093 1106 8 -0.20797064792603237 1106 9 -0.08174350694076793 1106 10-11 -0.0971641117250428 1106 12-13 -0.338695883907981 1106 14-15 -0.09499168120959922 1106 16-17 -0.05305462313232567 1106 18-19 -0.17912425278098 1106 20-21 -0.060784493263540185 1106 22-23 -0.02733257052707927 1106 24-25 -0.0898321301147007 1106 26-27 0.0056965825676442705 1106 28-29 -0.08942129478263183 1106 30-31 0.17981537104203227 1106 32-33 9.353053038978487E-4 1106 34-35 -0.32479927773220396 1106 36-37 0.006887459153119835 1106 38-39 0.0071143839210279225 1106 40-41 -0.014405064986505067 1106 42-43 -0.19700184743561522 1106 44-45 -0.04780769539734564 1106 46-47 0.15093104166193427 1106 48-49 -0.14289411165293586 1106 50-51 -0.10955550245702028 1106 52-53 -0.114869635659133 1106 54-55 -0.17996769442964222 1106 56-57 -0.24568931296007435 1106 58-59 -0.06390330966063118 1106 60-61 -0.08164000886144862 1106 62-63 -0.2576946112704235 1106 64-65 -0.5025238420433453 1106 66-67 -0.5610775129314902 1106 68-69 -0.1560908930449969 1106 70-71 -0.11477724121725785 1106 72-73 -0.04714531065607375 1106 74-75 -0.24013951625270025 1106 76-77 -0.19186407814984463 1106 78-79 -0.11141356872595054 1106 80-81 -0.1331967225031896 1106 82-83 -0.40862260594640887 1106 84-85 -0.5073856499549727 1106 86-87 -0.42259337341684855 1106 88-89 -0.241201222354249 1106 90-91 -0.3813958590952673 1106 92-93 -0.721999451169733 1106 94-95 -0.6233450091293982 1106 96-97 -0.32035355440991253 1106 98-99 -0.9311215578270797 1106 100-101 -0.8906407249023864 1107 1 0.057197650422569524 1107 2 0.09236391792853027 1107 3 0.0407672414388216 1107 4 -0.026094503623504295 1107 5 -0.05868803535999234 1107 6 -0.047988286935122915 1107 7 -0.03608011365354713 1107 8 0.029815167686336963 1107 9 0.03419067843291401 1107 10-11 0.09083717910936429 1107 12-13 0.17209972725505196 1107 14-15 0.14799052723774508 1107 16-17 0.03261106420337967 1107 18-19 0.1451967705638424 1107 20-21 0.002221559763754044 1107 22-23 0.0731200058753032 1107 24-25 0.12472875087977542 1107 26-27 0.12972705612011026 1107 28-29 0.05067793811213761 1107 30-31 0.1251459568701918 1107 32-33 0.09666602738680297 1107 34-35 -0.04100843772619811 1107 36-37 0.18303425122793726 1107 38-39 0.18338723404304602 1107 40-41 0.026106629838729134 1107 42-43 0.14646879392532242 1107 44-45 0.19590733049777498 1107 46-47 0.09929654500891871 1107 48-49 0.28148114466509355 1107 50-51 0.480165332192918 1107 52-53 0.4737312432736047 1107 54-55 0.2465550307548554 1107 56-57 0.3015219468808539 1107 58-59 0.34380483178434673 1107 60-61 0.3045290142503134 1107 62-63 0.3005666168599035 1107 64-65 0.2786231405191799 1107 66-67 0.23849691510113047 1107 68-69 0.5361000070206607 1107 70-71 0.3568955546717909 1107 72-73 0.51926599041985 1107 74-75 0.3474232046290098 1107 76-77 0.26825451726097427 1107 78-79 0.24046318348090878 1107 80-81 0.3176333557312354 1107 82-83 0.21354613031326508 1107 84-85 0.35811153418838515 1107 86-87 0.16092764634232637 1107 88-89 0.19718888752354857 1107 90-91 0.41768666242424146 1107 92-93 0.37073725222744613 1107 94-95 0.31751247692578133 1107 96-97 0.23379369931748073 1107 98-99 0.2142172077676996 1107 100-101 0.36849204055345197 1108 1 0.11173222947771322 1108 2 -0.041547506651724575 1108 3 -0.1096955006659428 1108 4 -0.10543465270890806 1108 5 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2316 82-83 -0.38909869443277856 2316 84-85 -0.4300401390369579 2316 86-87 -0.6358326721348391 2316 88-89 -0.8311720532653233 2316 90-91 -0.5546189414316522 2316 92-93 -0.41432726685372856 2316 94-95 -0.6175703454408357 2316 96-97 -0.534117590759088 2316 98-99 -0.3857126296381139 2316 100-101 -0.056308911217392676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 15.0 5 43.0 6 57.0 7 162.0 8 401.0 9 793.0 10 1242.0 11 2109.0 12 3206.0 13 4922.0 14 6297.0 15 9109.0 16 12509.0 17 18029.0 18 15877.0 19 9253.0 20 7533.0 21 7514.0 22 7903.0 23 8681.0 24 10184.0 25 11963.0 26 14520.0 27 17728.0 28 21719.0 29 27025.0 30 33682.0 31 42133.0 32 53967.0 33 71019.0 34 97506.0 35 138229.0 36 200820.0 37 269588.0 38 165993.0 39 9883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.428592065639926 20.039680252773355 15.50242315408832 21.0293045274984 2 15.776020823265757 21.490741160445324 39.64256876617694 23.090669250111976 3 20.007821017024156 25.559052904032743 30.431285297333094 24.001840781610007 4 12.720215792461861 17.609734323585744 37.4431574167903 32.2268924671621 5 13.087062114760903 38.60576110180303 33.77015540218578 14.537021381250288 6 30.81204129312603 37.326687261499316 16.837620167468103 15.02365127790655 7 27.747016988816252 34.33050019936679 20.19243696897945 17.73004584283751 8 26.637016458708025 34.7904651200311 19.110017432109654 19.46250098915122 9 26.60313562857391 17.254638639050846 20.6139114227567 35.528314309618544 10-11 25.178642630996272 27.384737953674687 26.26728517748254 21.169334237846503 12-13 26.61356372353176 24.86324103383632 27.465656596699343 21.05753864593257 14-15 22.992791296391193 25.999004318461367 27.060718903582533 23.947485481564907 16-17 21.13783505336807 29.48957492234683 27.624477478467234 21.748112545817868 18-19 21.070034979460228 27.437210985379 31.025813197543677 20.466940837617095 20-21 22.21656688083551 25.936351672260972 31.27842129160485 20.568660155298662 22-23 21.61153123086509 26.046259525680824 31.148352083146065 21.19385716030802 24-25 21.776695706153532 26.273750718816615 31.393223129511448 20.556330445518405 26-27 21.44428831218121 26.379510495903897 31.61371017122733 20.562491020687567 28-29 20.845598587288595 27.079299068346486 31.535701348444867 20.539400995920055 30-31 21.09738549086101 26.528997304130776 31.37676020900093 20.99685699600729 32-33 21.017024156801405 26.6711661843194 31.268126436189952 21.043683222689246 34-35 21.683577631276695 26.334355392237836 31.17101675526132 20.81105022122415 36-37 21.7007485281814 26.32425260502097 31.2145009952974 20.76049787150023 38-39 21.350723444074735 26.73904308550646 31.294055642925244 20.616177827493566 40-41 21.31520911977892 26.709142251080486 31.151869165161163 20.82377946397943 42-43 21.1819753144285 26.959537776562037 30.554954729288177 21.303532179721287 44-45 21.59829935134728 26.848598785668027 30.291152340043688 21.26194952294101 46-47 21.86508207842375 27.33894889526214 29.70999238641663 21.08597663989748 48-49 22.02857052859473 27.52168257887665 29.299581060846435 21.150165831682187 50-51 21.71465426571944 27.823536958915074 29.347636523995845 21.114172251369638 52-53 22.026188882939042 28.061701524483702 28.70386233780801 21.208247254769244 54-55 21.496404480570366 27.840306694734256 28.980731703569397 21.68255712112598 56-57 22.000912708077085 28.00957421553586 28.70420806056448 21.28530501582258 58-59 22.256632306946493 27.53966016221312 28.760023078914678 21.443684451925716 60-61 21.917900832884534 27.67971629222331 29.460226901686436 20.94215597320572 62-63 21.698290055246495 27.618600191607236 29.454272787547218 21.228836965599047 64-65 21.56553251676179 27.843742533348802 29.16966613169271 21.421058818196695 66-67 21.621693257868458 27.901900783715078 28.75057332357115 21.725832634845318 68-69 21.583125963702184 27.84773755186802 28.955356436320557 21.613780048109245 70-71 21.88202249349081 27.974886698969975 28.653732538119776 21.48935826941944 72-73 21.908143768424143 27.631737656353117 28.906417459460165 21.55370111576258 74-75 21.6554287520825 28.418959289593243 28.30729079635731 21.618321161966946 76-77 21.802851982259046 27.840823096738603 28.545559728984088 21.810765192018263 78-79 21.737087831250417 28.02666828516135 28.484136319251146 21.752107564337084 80-81 21.6767400034265 28.29322053040017 28.439307601828794 21.590731864344537 82-83 21.82532121707544 28.02229082092052 28.456521605516848 21.695866356487187 84-85 21.573769610178086 28.04001936048924 28.37467924586285 22.011531783469827 86-87 21.631373495049637 28.065927024840565 28.32598736498566 21.97671211512414 88-89 21.523277513193165 27.971045335009194 28.22376866998919 22.28190848180845 90-91 21.590135913945115 27.868717048734542 28.40738607789705 22.133760959423288 92-93 21.781177169272873 28.070217896282706 28.4588149020575 21.689790032386924 94-95 21.457820671301402 28.013031443100566 28.249851531282914 22.27929635431512 96-97 21.30412769923042 27.92337400825586 28.447643361623697 22.32485493089002 98-99 21.49940726843326 28.098165259553426 27.83694849650546 22.565478975507855 100-101 21.33677692618611 27.75428515223375 28.299179162626004 22.609758758954136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 416.5 2 352.5 3 477.0 4 411.0 5 360.0 6 702.0 7 1622.0 8 2542.5 9 3005.0 10 2802.0 11 2441.0 12 2491.0 13 2688.5 14 2910.5 15 3083.5 16 3259.0 17 3339.5 18 3434.0 19 3614.0 20 3917.0 21 4367.0 22 4941.5 23 5706.5 24 6680.0 25 7960.0 26 9615.5 27 11636.5 28 13218.5 29 15100.5 30 17633.5 31 19797.5 32 21924.5 33 24194.5 34 26946.0 35 29514.0 36 32765.0 37 35822.5 38 37749.5 39 38372.0 40 38377.0 41 40191.0 42 41958.5 43 43372.5 44 44661.5 45 45615.5 46 45711.0 47 48897.0 48 59739.0 49 58879.0 50 48264.5 51 43669.5 52 40528.0 53 38667.0 54 36829.0 55 33976.0 56 30341.0 57 26588.5 58 23836.5 59 21376.5 60 18505.0 61 15179.0 62 12013.5 63 9171.5 64 6658.5 65 5133.0 66 3890.5 67 2983.5 68 2157.5 69 1382.0 70 993.0 71 829.0 72 703.5 73 586.5 74 472.0 75 385.0 76 299.0 77 201.5 78 132.0 79 100.0 80 74.5 81 58.5 82 48.0 83 33.5 84 24.0 85 14.0 86 5.5 87 2.0 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016748346869019475 2 0.0 3 0.0 4 0.0 5 0.03695392130274481 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004686464032156826 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.146182337254854E-4 24-25 1.920681980392142E-4 26-27 0.001382891025882342 28-29 8.066864317646995E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.066864317646995E-4 42-43 0.013252705664705777 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.682727921568568E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.841363960784284E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.91445512941171E-4 84-85 7.682727921568568E-5 86-87 0.0 88-89 0.0 90-91 0.013252705664705777 92-93 0.0011908228278431278 94-95 0.0 96-97 0.0 98-99 0.0015365455843137136 100-101 0.020512883550588074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1301621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.836978729901844 #Duplication Level Percentage of deduplicated Percentage of total 1 76.58061585966759 41.99449603019376 2 13.494312004070865 14.799746006837863 3 4.385023396713846 7.2138430420715745 4 1.8883856573562692 4.142134565051898 5 1.0034064605856363 2.7511889368290317 6 0.5678239018932114 1.8682648338268746 7 0.3793483817656807 1.456162339947512 8 0.26435403316717765 1.1597101195161832 9 0.19334373010408182 0.9542147413749676 >10 1.1029761178743223 11.284144373061379 >50 0.09164614170160407 3.478627733545207 >100 0.04385987588549082 4.477323881642926 >500 0.0028025366937915307 1.0010119086453089 >1k 0.001541395181585342 1.1735206777332583 >5k 5.605073387583061E-4 2.2456108097221548 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9493 0.729321361594504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8487 0.6520331187035243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5917 0.4545870111192121 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5327 0.40925891638195755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2067 0.1588019861388223 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1745 0.1340636022313715 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1686 0.12953079275764603 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1566 0.12031151925176377 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1396 0.1072508817850972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.841363960784284E-5 0.0 18-19 0.0 0.0 0.0 7.682727921568568E-5 0.0 20-21 0.0 0.0 0.0 7.682727921568568E-5 0.0 22-23 0.0 0.0 0.0 1.1524091882352851E-4 0.0 24-25 0.0 0.0 0.0 2.688954772548999E-4 0.0 26-27 0.0 0.0 0.0 5.377909545097998E-4 0.0 28-29 0.0 0.0 0.0 0.0011139955486274423 0.0 30-31 0.0 0.0 0.0 0.0028810229705882127 0.0 32-33 0.0 0.0 0.0 0.005262668626274468 0.0 34-35 0.0 0.0 0.0 0.00799003703843131 0.0 36-37 0.0 0.0 0.0 0.012676501070588136 0.0 38-39 0.0 0.0 0.0 0.019168406164313576 0.0 40-41 0.0 0.0 0.0 0.028349266030588013 0.0 42-43 0.0 0.0 0.0 0.04144831713686242 0.0 44-45 0.0 0.0 0.0 0.05427847276588193 0.0 46-47 0.0 0.0 0.0 0.07870954755646997 0.0 48-49 0.0 0.0 0.0 0.10398572241843056 0.0 50-51 0.0 0.0 0.0 0.12376874681646961 0.0 52-53 0.0 0.0 0.0 0.1523869083243125 0.0 54-55 0.0 0.0 0.0 0.1893792432666652 0.0 56-57 0.0 0.0 0.0 0.2421211704482334 0.0 58-59 0.0 0.0 0.0 0.304543334810978 3.841363960784284E-5 60-61 0.0 0.0 0.0 0.3820620595396048 7.682727921568568E-5 62-63 0.0 0.0 0.0 0.4655733120470552 7.682727921568568E-5 64-65 0.0 0.0 0.0 0.5610312064725447 7.682727921568568E-5 66-67 0.0 0.0 0.0 0.6671296790694066 7.682727921568568E-5 68-69 0.0 0.0 0.0 0.7939331034148958 7.682727921568568E-5 70-71 0.0 0.0 0.0 0.9292259421137181 7.682727921568568E-5 72-73 0.0 0.0 0.0 1.0719326132568543 1.5365455843137135E-4 74-75 0.0 0.0 0.0 1.2237817306266572 1.5365455843137135E-4 76-77 0.0 0.0 0.0 1.4039417003874402 1.5365455843137135E-4 78-79 0.0 0.0 0.0 1.616330713779203 1.5365455843137135E-4 80-81 0.0 0.0 0.0 1.8752770583756715 1.5365455843137135E-4 82-83 0.0 0.0 0.0 2.148013899591356 1.5365455843137135E-4 84-85 0.0 0.0 0.0 2.4535944026717456 1.5365455843137135E-4 86-87 0.0 0.0 0.0 2.7771524890886057 1.5365455843137135E-4 88-89 0.0 0.0 0.0 3.1657064537219357 1.5365455843137135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7395 0.0 22.429 1 GAGTACT 7995 0.0 22.189344 12-13 CGCGGAA 120 5.9426384E-9 21.76893 82-83 ATTCGCG 120 5.9426384E-9 21.76893 80-81 TACATGG 7620 0.0 21.763382 2 AGTACTT 8195 0.0 21.415976 12-13 GTATCAA 14930 0.0 21.104565 1 GTACTTT 8540 0.0 20.93933 14-15 ACATGGG 7470 0.0 20.864557 3 GGTATCA 11520 0.0 20.792213 1 CGATCGT 140 1.5788828E-9 20.356148 24-25 CCCGCGA 140 1.5788828E-9 20.356148 20-21 ACACGCT 260 1.4913894E-8 20.095171 9 CCTATAC 335 3.45608E-11 19.858166 3 CGCTAGA 120 0.004749317 19.802109 1 TCGCGGA 120 1.4162106E-7 19.789938 82-83 CGCGTAA 85 7.964133E-5 19.557114 64-65 AGAGTAC 13430 0.0 19.045048 10-11 TACTTTT 9260 0.0 18.875242 14-15 ACTTTTT 9570 0.0 18.710844 16-17 >>END_MODULE