FastQCFastQC Report
Thu 26 May 2016
SRR937530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146856
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80520.7020933752798956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62340.5435730379402471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.3496515691595109No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30930.2696938412494681No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25310.2206903046241202No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18540.16165935392063172No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16380.14282525443473287No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.13925026332861318No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15030.13105394225604608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107050.027.6760181
GTACATG64550.025.9327531
ACTGCGT1309.997981E-625.5845138
GGTATCA75350.025.4345471
TACATGG65000.025.0815892
GAGTACT62650.024.2631412-13
ACATGGG66400.023.7632793
ATCAACG125200.023.5734773
TCAACGC125050.023.5678674
TATCAAC126450.023.455252
CAACGCA125950.023.4361745
AACGCAG127600.023.1220026
GTACTTT66800.022.53997614-15
AGTACTT65400.021.2817812-13
TAACGCA1353.7771664E-421.126614
ACGCAGA139850.021.029637
CGCAGAG140300.020.8953658
CATGGGA41650.020.885674
GTGTAGG4550.019.8584081
GCAGAGT143950.019.8044179
Produced by FastQC (version 0.11.5)