##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937530_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1146856 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86289909108031 33.0 31.0 34.0 30.0 34.0 2 31.99891529538146 33.0 31.0 34.0 30.0 34.0 3 32.0255411315806 34.0 31.0 34.0 30.0 34.0 4 35.605344524508745 37.0 35.0 37.0 33.0 37.0 5 35.59202201496962 37.0 35.0 37.0 33.0 37.0 6 35.67030560070314 37.0 35.0 37.0 35.0 37.0 7 35.61869668031557 37.0 35.0 37.0 33.0 37.0 8 35.61007223225933 37.0 35.0 37.0 33.0 37.0 9 37.24179757528408 39.0 37.0 39.0 34.0 39.0 10-11 37.133419976003964 39.0 37.0 39.0 34.0 39.0 12-13 37.02578963705993 39.0 37.0 39.0 33.0 39.0 14-15 38.17266814665486 40.0 38.0 41.0 33.0 41.0 16-17 38.134468058762394 40.0 38.0 41.0 33.0 41.0 18-19 38.117226574216815 40.0 38.0 41.0 33.5 41.0 20-21 37.91616253479077 40.0 38.0 41.0 33.0 41.0 22-23 37.81949608320487 40.0 38.0 41.0 33.0 41.0 24-25 37.68549059341365 40.0 37.5 41.0 32.5 41.0 26-27 37.765075999079215 40.0 38.0 41.0 33.0 41.0 28-29 37.71412758009724 40.0 38.0 41.0 33.0 41.0 30-31 37.48156089343388 40.0 37.0 41.0 32.5 41.0 32-33 37.218053530696096 39.5 36.5 41.0 32.0 41.0 34-35 36.95028408100058 39.0 36.0 41.0 31.0 41.0 36-37 36.97571273115369 39.0 36.0 41.0 31.0 41.0 38-39 36.9256676513878 40.0 36.0 41.0 31.0 41.0 40-41 36.73180024344818 39.5 36.0 41.0 31.0 41.0 42-43 36.4661544256646 39.0 35.5 41.0 30.0 41.0 44-45 36.181854565874005 39.0 35.0 41.0 30.0 41.0 46-47 35.7468034347817 39.0 35.0 41.0 28.0 41.0 48-49 35.349734840293806 39.0 35.0 40.5 26.5 41.0 50-51 34.408457992982555 38.0 34.0 39.5 24.5 40.5 52-53 34.49487904322774 38.0 34.5 39.5 24.0 40.5 54-55 34.79505622327476 39.0 35.0 40.0 24.0 41.0 56-57 34.56168036789274 38.0 34.0 40.0 23.5 41.0 58-59 34.33255352023271 38.0 34.0 40.0 23.0 41.0 60-61 34.02075805506533 38.0 34.0 40.0 22.0 41.0 62-63 33.66939572186918 37.5 33.5 40.0 21.0 41.0 64-65 33.30598348877278 37.0 33.0 40.0 19.5 41.0 66-67 32.89341425601819 36.5 32.5 39.0 18.0 41.0 68-69 32.43607218343018 36.0 32.0 39.0 14.5 40.5 70-71 31.992853941558486 35.0 31.5 39.0 10.0 40.0 72-73 31.52751260838327 35.0 31.0 38.0 7.5 40.0 74-75 31.053862908682518 35.0 31.0 37.0 6.0 39.0 76-77 30.608672318059114 35.0 30.5 37.0 2.0 39.0 78-79 30.097529245171145 34.0 30.0 36.0 2.0 39.0 80-81 29.56237443933676 34.0 29.0 36.0 2.0 37.5 82-83 29.054578778852793 34.0 29.0 35.0 2.0 37.0 84-85 28.598082496843546 34.0 29.0 35.0 2.0 36.5 86-87 28.115577282588227 34.0 27.5 35.0 2.0 36.0 88-89 27.630451425462304 33.5 26.5 35.0 2.0 36.0 90-91 27.124668659360896 33.0 25.5 35.0 2.0 35.0 92-93 26.59671746060534 33.0 25.0 35.0 2.0 35.0 94-95 25.9887418298374 32.5 23.5 35.0 2.0 35.0 96-97 25.237159242311154 32.0 19.5 34.0 2.0 35.0 98-99 24.356008513710528 32.0 9.0 34.0 2.0 35.0 100-101 21.858254218489506 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2904000693033275 1101 2 0.3630163579477035 1101 3 0.32732567835592974 1101 4 0.2997488994163575 1101 5 0.26480537339361376 1101 6 0.3167900115629152 1101 7 0.2418513964440976 1101 8 0.3555613978675254 1101 9 0.4316459779153021 1101 10-11 0.4421322959656635 1101 12-13 0.4634981698616798 1101 14-15 0.603076599236573 1101 16-17 0.6161558612079716 1101 18-19 0.5712401498130859 1101 20-21 0.6276932537805209 1101 22-23 0.6768161319264792 1101 24-25 0.6032932155692876 1101 26-27 0.628640920733254 1101 28-29 0.5422742942506531 1101 30-31 0.605572622340091 1101 32-33 0.4791748938002556 1101 34-35 0.5218931477184086 1101 36-37 0.5719324439654798 1101 38-39 0.41281350511908244 1101 40-41 0.5354067557648747 1101 42-43 0.5191407221389142 1101 44-45 0.5697133810149566 1101 46-47 0.6448581563685138 1101 48-49 0.6651659034764492 1101 50-51 0.689922766522578 1101 52-53 0.7012244895195394 1101 54-55 0.7538519078236874 1101 56-57 0.9134434741185444 1101 58-59 0.8271813065252616 1101 60-61 0.7973528552989606 1101 62-63 0.8683489580617376 1101 64-65 0.7970078944385435 1101 66-67 0.8362752846976846 1101 68-69 1.0133369575224762 1101 70-71 0.9963787817240863 1101 72-73 1.1215646661325245 1101 74-75 1.114018025586141 1101 76-77 1.0259484780315482 1101 78-79 1.0880228045889275 1101 80-81 1.057193568340601 1101 82-83 1.21357852047068 1101 84-85 1.215248690575688 1101 86-87 1.2174701478158418 1101 88-89 1.208014699657447 1101 90-91 1.2275563418689082 1101 92-93 1.4765483651700997 1101 94-95 1.4511882531268618 1101 96-97 1.6129292079418356 1101 98-99 1.788314726351853 1101 100-101 2.0530408088300725 1102 1 0.1273841412331045 1102 2 0.24784085950468793 1102 3 0.2283946008957365 1102 4 0.14497571910023765 1102 5 0.11996008034990524 1102 6 0.15714436252930852 1102 7 0.19033624781641123 1102 8 0.11922403455583463 1102 9 0.19313275026090793 1102 10-11 0.20497594627228466 1102 12-13 0.2736565072288002 1102 14-15 0.4450053193118819 1102 16-17 0.446107322329226 1102 18-19 0.4341035243990703 1102 20-21 0.466383428230813 1102 22-23 0.4461328238122704 1102 24-25 0.4578018809121076 1102 26-27 0.4743012934173194 1102 28-29 0.4314552888117049 1102 30-31 0.3269445460195186 1102 32-33 0.23761798115499033 1102 34-35 0.43829865031591453 1102 36-37 0.38832084265890643 1102 38-39 0.42182497569275057 1102 40-41 0.4969153486900524 1102 42-43 0.5176742648481678 1102 44-45 0.48580801181837074 1102 46-47 0.5699616373703194 1102 48-49 0.4499844972782583 1102 50-51 0.4011464531904849 1102 52-53 0.5049157407058544 1102 54-55 0.5691454660836825 1102 56-57 0.6399765842276608 1102 58-59 0.5958791134140853 1102 60-61 0.5253043030801265 1102 62-63 0.4886558659426825 1102 64-65 0.6573761953300448 1102 66-67 0.6711713939644781 1102 68-69 0.6543050480216621 1102 70-71 0.7045880839610241 1102 72-73 0.5078773026302521 1102 74-75 0.562686303227764 1102 76-77 0.45954650102986605 1102 78-79 0.6869646017661601 1102 80-81 0.9152850013333165 1102 82-83 0.9021812220299452 1102 84-85 0.8360756238409266 1102 86-87 0.807524584921115 1102 88-89 0.8996598951279147 1102 90-91 0.9994633938228468 1102 92-93 1.0807749828468651 1102 94-95 0.8841935948809656 1102 96-97 1.1585513707900574 1102 98-99 1.3620809107424172 1102 100-101 1.4748266565167114 1103 1 0.1644896453796605 1103 2 0.22495498313136508 1103 3 0.22889220740590233 1103 4 0.11282138715771595 1103 5 0.2160139777479202 1103 6 0.26340912540057104 1103 7 0.199145064830482 1103 8 0.16010496151704956 1103 9 0.02063100398642348 1103 10-11 0.12395597613371478 1103 12-13 0.09345682092960317 1103 14-15 0.3460940189924031 1103 16-17 0.23675101243225782 1103 18-19 0.1585629084934581 1103 20-21 0.27408415927738616 1103 22-23 0.26565843558665847 1103 24-25 0.22670200205754298 1103 26-27 0.0387725154674925 1103 28-29 0.16611309193050516 1103 30-31 0.11432638647465154 1103 32-33 0.29770931043069737 1103 34-35 0.22752885128457478 1103 36-37 0.24916024122638447 1103 38-39 0.4059976187502201 1103 40-41 0.36182360453271656 1103 42-43 0.3352291888896275 1103 44-45 0.31770715384316617 1103 46-47 0.35535342894987565 1103 48-49 0.2406064375262602 1103 50-51 0.19483286000883737 1103 52-53 0.26097502402172523 1103 54-55 0.2869150725093661 1103 56-57 0.4992616853506604 1103 58-59 0.5799788378823933 1103 60-61 0.6313185025924284 1103 62-63 0.5561975892050697 1103 64-65 0.5582658775508378 1103 66-67 0.685324514955532 1103 68-69 0.6984800093873744 1103 70-71 0.8373906873055255 1103 72-73 0.7103536179181695 1103 74-75 0.6722276251864692 1103 76-77 0.5518370448969385 1103 78-79 0.6316861508184992 1103 80-81 0.6946469284760788 1103 82-83 0.5826552330904384 1103 84-85 0.6540479439444766 1103 86-87 0.5658730090498274 1103 88-89 0.7586057990896045 1103 90-91 0.8176006608039508 1103 92-93 0.9265891289525854 1103 94-95 0.8663502903915443 1103 96-97 0.9949689509087314 1103 98-99 1.0787705677182906 1103 100-101 1.3635311396018608 1104 1 0.03586766078861814 1104 2 0.059011278278909174 1104 3 0.2046021330660146 1104 4 0.11447168161701882 1104 5 0.07206297866007105 1104 6 0.13035079105289782 1104 7 0.0757186075236973 1104 8 0.12658934743529926 1104 9 0.20793326393710032 1104 10-11 0.24382532907462462 1104 12-13 0.15107106196997222 1104 14-15 0.24030040648250406 1104 16-17 0.3213576812328043 1104 18-19 0.28150398507691676 1104 20-21 0.2102985202353409 1104 22-23 0.2450299051181304 1104 24-25 0.32328074418513353 1104 26-27 0.3394730866539106 1104 28-29 0.25496150738034373 1104 30-31 0.08733891771258584 1104 32-33 0.26704647271165527 1104 34-35 0.3300033913374989 1104 36-37 0.36953526170103856 1104 38-39 0.4346229093399927 1104 40-41 0.47954150366068404 1104 42-43 0.4706851236833103 1104 44-45 0.6580603246716308 1104 46-47 0.6304024554083938 1104 48-49 0.5321212912283002 1104 50-51 0.4145998772616366 1104 52-53 0.5160248619526513 1104 54-55 0.5826815908569145 1104 56-57 0.6162707482953707 1104 58-59 0.5972393070036119 1104 60-61 0.7052504020239425 1104 62-63 0.6463553304265162 1104 64-65 0.8268594906618247 1104 66-67 0.9310209311026227 1104 68-69 0.9388751062907161 1104 70-71 0.9696336421470839 1104 72-73 0.9630404181171279 1104 74-75 0.8087817526507521 1104 76-77 0.8183755289763006 1104 78-79 0.7623840020695916 1104 80-81 0.6368774557678485 1104 82-83 0.7795285096507563 1104 84-85 0.9767832972430384 1104 86-87 0.909900046108568 1104 88-89 0.8661971235478383 1104 90-91 1.1388382464030222 1104 92-93 1.0088620631917635 1104 94-95 1.119603618555498 1104 96-97 1.2088096648842424 1104 98-99 1.267912766237501 1104 100-101 1.3622350378251866 1105 1 0.057041625883702096 1105 2 0.06548611429237639 1105 3 0.16428881902403347 1105 4 0.1067443113573816 1105 5 0.14745848148859864 1105 6 0.06804332858781947 1105 7 0.16683315444760893 1105 8 0.14017230526905422 1105 9 0.24396076915599707 1105 10-11 0.2435209187342977 1105 12-13 0.23287061237314077 1105 14-15 0.09781250368525463 1105 16-17 0.20436083562980656 1105 18-19 0.1340985919203419 1105 20-21 0.27323846637195714 1105 22-23 0.1921858342013465 1105 24-25 0.20099321478186027 1105 26-27 0.17858560417555225 1105 28-29 0.16416560869804897 1105 30-31 0.13754155713873217 1105 32-33 0.13059544006274137 1105 34-35 0.24521307952746696 1105 36-37 0.19792984809000558 1105 38-39 0.3023311486730975 1105 40-41 0.36824543352197026 1105 42-43 0.3195634377553773 1105 44-45 0.5107937009192085 1105 46-47 0.5743951639986591 1105 48-49 0.5101253380008046 1105 50-51 0.4522029767123783 1105 52-53 0.30868412089997577 1105 54-55 0.4202225248231244 1105 56-57 0.6079666427664563 1105 58-59 0.6631393328724755 1105 60-61 0.6185867032578258 1105 62-63 0.5400499454918233 1105 64-65 0.6202324494781948 1105 66-67 0.6354094132420798 1105 68-69 0.5857206090189706 1105 70-71 0.6280262833579862 1105 72-73 0.6331334056499394 1105 74-75 0.5415467731352805 1105 76-77 0.5272449393467049 1105 78-79 0.6134433219791937 1105 80-81 0.7853500899199624 1105 82-83 0.8070900785471586 1105 84-85 0.8519941084223959 1105 86-87 0.7733567679019835 1105 88-89 0.6117281270792354 1105 90-91 0.7464815929916391 1105 92-93 0.8909668260705459 1105 94-95 0.9267825288146092 1105 96-97 1.2793382118763326 1105 98-99 1.4058940724747053 1105 100-101 1.2994188210561468 1106 1 0.0025313007715759284 1106 2 0.11317634429553536 1106 3 0.07862690065163136 1106 4 0.024703036663311195 1106 5 -0.09588118135371104 1106 6 -0.05790334793707075 1106 7 -0.024501874743428687 1106 8 -0.01329512637573771 1106 9 0.17396130034111934 1106 10-11 -0.08875040204411278 1106 12-13 -0.30136856151057856 1106 14-15 -0.12633715745619156 1106 16-17 -0.19605483014636604 1106 18-19 -0.30321056372602584 1106 20-21 -0.3371764106747861 1106 22-23 -0.3558410813246553 1106 24-25 -0.33190431424150546 1106 26-27 -0.17748495652522678 1106 28-29 -0.026066540664771765 1106 30-31 0.08879405411124708 1106 32-33 0.0925132222628875 1106 34-35 -0.0024501083237424837 1106 36-37 -0.03063213719025981 1106 38-39 0.1162481453631159 1106 40-41 -0.08117796233917574 1106 42-43 -0.21329277686917436 1106 44-45 -0.0325872226668622 1106 46-47 0.1930380450180209 1106 48-49 0.24724423279234742 1106 50-51 0.17053508760451308 1106 52-53 0.15692560302451852 1106 54-55 0.20786383213947346 1106 56-57 0.32641223411769005 1106 58-59 0.1538827613344509 1106 60-61 -0.02228520225345676 1106 62-63 0.2398812539724915 1106 64-65 0.18343891899111497 1106 66-67 0.5251005049455628 1106 68-69 0.5770888506942597 1106 70-71 0.2087393269575557 1106 72-73 0.34213651066845685 1106 74-75 0.09786473733340273 1106 76-77 0.3100589006088228 1106 78-79 0.49171768929209847 1106 80-81 0.29802368417071534 1106 82-83 0.04341970735606537 1106 84-85 0.0620670954051441 1106 86-87 -0.10859136160202354 1106 88-89 0.1863338830871264 1106 90-91 0.4128085336818721 1106 92-93 0.332787299447709 1106 94-95 0.2654774337834418 1106 96-97 0.15774671684773978 1106 98-99 0.5504536904935762 1106 100-101 0.6196684566041277 1107 1 0.09570588689164339 1107 2 0.14421461278977077 1107 3 0.17596108814794675 1107 4 0.25347464958529997 1107 5 0.23930723671328735 1107 6 0.15288802652479916 1107 7 0.22318707852185327 1107 8 0.13231031010774075 1107 9 0.16849702694649693 1107 10-11 0.2008936527311107 1107 12-13 0.2953807451552066 1107 14-15 0.3204035456448793 1107 16-17 0.3388744307791498 1107 18-19 0.34581650640070194 1107 20-21 0.47871215175437953 1107 22-23 0.5920847325200498 1107 24-25 0.40536786296122784 1107 26-27 0.46947281491723913 1107 28-29 0.3527936526205693 1107 30-31 0.3633931805060939 1107 32-33 0.5840263913718147 1107 34-35 0.6013759332528821 1107 36-37 0.6749579178967835 1107 38-39 0.5676392165594351 1107 40-41 0.6036940626652765 1107 42-43 0.5867547245021427 1107 44-45 0.4371637748215491 1107 46-47 0.4629863993944028 1107 48-49 0.5457899476112402 1107 50-51 0.6410244567491432 1107 52-53 0.5336958975411008 1107 54-55 0.5555363053371494 1107 56-57 0.472094819937233 1107 58-59 0.39572573375699704 1107 60-61 0.5897396729544582 1107 62-63 0.5341797160752009 1107 64-65 0.5424523445912826 1107 66-67 0.6355492717275624 1107 68-69 0.913496712979061 1107 70-71 0.425711938539326 1107 72-73 0.45075550523892005 1107 74-75 0.6359599485004281 1107 76-77 0.6900868359390948 1107 78-79 0.9314745598241991 1107 80-81 1.1085954051825588 1107 82-83 1.0474629788433667 1107 84-85 1.0516361820297107 1107 86-87 1.2591698292928974 1107 88-89 1.0663791871161017 1107 90-91 1.2077561004079662 1107 92-93 1.1945098381213377 1107 94-95 0.9789653996987866 1107 96-97 1.0906760730991394 1107 98-99 1.442348082342729 1107 100-101 1.4385432234394493 1108 1 0.046864114576038673 1108 2 0.03106062707500712 1108 3 0.09657842775308367 1108 4 0.0654072214826229 1108 5 -0.028636147033459736 1108 6 -0.11929086043853232 1108 7 -0.013021901299232752 1108 8 -0.02087079760799071 1108 9 0.09497201669260136 1108 10-11 0.08283766094900358 1108 12-13 -0.004794032861930475 1108 14-15 0.07044188226069537 1108 16-17 0.06271684973646785 1108 18-19 0.19681686112986796 1108 20-21 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-0.20757871251925764 2316 68-69 -0.19610535280816066 2316 70-71 0.07655830860411683 2316 72-73 -0.03263662544186374 2316 74-75 -0.2955913662567511 2316 76-77 -0.14040664612814524 2316 78-79 -0.05624424164512476 2316 80-81 -0.08721924548845905 2316 82-83 -0.2662810526630075 2316 84-85 -0.031130393366847642 2316 86-87 0.43147290147595285 2316 88-89 0.27049387571530303 2316 90-91 0.059758241012428215 2316 92-93 0.1456461250039034 2316 94-95 0.1532454136231074 2316 96-97 0.009882542712428943 2316 98-99 0.5771905764586656 2316 100-101 0.8512629341461064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4762.0 3 969.0 4 881.0 5 952.0 6 1051.0 7 1191.0 8 1435.0 9 1772.0 10 2204.0 11 2800.0 12 3943.0 13 5333.0 14 6357.0 15 8203.0 16 10930.0 17 15282.0 18 12969.0 19 7935.0 20 7294.0 21 7597.0 22 8081.0 23 8757.0 24 9993.0 25 11390.0 26 13242.0 27 15360.0 28 18322.0 29 22023.0 30 27135.0 31 33942.0 32 43658.0 33 57912.0 34 79352.0 35 113590.0 36 162761.0 37 220816.0 38 173910.0 39 22749.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.463057160477085 18.93761727104537 14.932053867046891 21.66727170143065 2 15.047288630706266 20.183856027824966 41.33971484304301 23.429140498425753 3 19.539334487401426 25.471506758292676 30.21000542062285 24.779153333683052 4 12.083185786544608 16.64100445482008 38.19297808438365 33.082831674251665 5 12.937262274297206 38.00419708827001 34.20522012853583 14.853320508896953 6 30.45007401038417 36.53430429365084 17.83239575196123 15.18322594400376 7 27.522985477843687 33.97097665673243 20.747959637474693 17.758078227949188 8 26.207678079492823 34.219606109402015 19.91514428180626 19.6575715292989 9 26.56420805017839 17.03421102991357 21.082194378349474 35.319386541558565 10-11 24.852087034252197 26.927805540433912 26.913955766355624 21.30615165895827 12-13 26.237945156524013 24.592317579428496 27.749054791923157 21.420682472124337 14-15 22.933879152349476 25.491116970267573 27.32866882343818 24.246335053944772 16-17 21.1291637290028 29.17491178271859 27.776146955706583 21.91977753257203 18-19 20.914419664465534 27.221330080851892 31.11386713869585 20.750383115986725 20-21 22.300787546027546 25.837537509991744 30.90583081082734 20.955844133153374 22-23 21.569701560475526 25.946214660833995 31.07160049869516 21.412483279995318 24-25 21.568295108994082 26.06094547303575 31.277966032096476 21.09279338587369 26-27 21.272331413554017 26.13753914113174 31.4641841170879 21.125945328226347 28-29 20.767345041380988 26.68222904660107 31.44915815913036 21.10126775288758 30-31 20.95731444119595 26.309130403630704 31.288063736498025 21.445491418675324 32-33 20.833458943745324 26.44009606684309 31.224957106774966 21.50148788263662 34-35 21.55618408026709 26.309394929920078 30.917648991375113 21.216771998437718 36-37 21.421782150538764 26.12509194854027 31.181693810050476 21.271432090870494 38-39 21.185711883542723 26.515519120193748 31.14996320309924 21.148805793164293 40-41 21.21063363959196 26.55846217610606 31.14102993566342 21.089874248638562 42-43 21.024698632823675 26.693682002099315 30.494160485419886 21.78745887965712 44-45 21.132497795103404 26.74348457970237 30.282108820328997 21.84190880486523 46-47 21.467520809353342 27.01710396704576 29.91300587314103 21.602369350459867 48-49 21.628934288034817 27.124060076465245 29.48831373206931 21.758691903430623 50-51 21.418757304033463 27.4267303050492 29.547767013619637 21.6067453772977 52-53 21.719592648944175 27.59826999023833 29.019655308925945 21.662482051891555 54-55 21.27580758526665 27.313197923642353 29.318351686898612 22.09264280419238 56-57 21.613262062167333 27.550871112248593 29.023972565347066 21.811894260237004 58-59 21.87243680845693 27.20479239840917 29.155067246790523 21.767703546343377 60-61 21.67351920712868 27.17776501645265 29.761103626569664 21.387612149849012 62-63 21.475117228596748 27.310146884582075 29.60178743857973 21.61294844824145 64-65 21.247168434425372 27.451828993746645 29.538158389491535 21.762844182336448 66-67 21.29302009518727 27.500281683743832 29.063866657466452 22.142831563602442 68-69 21.14332681585106 27.396441253784822 29.413221332212974 22.047010598151143 70-71 21.43046811127338 27.503327929451387 29.097327524160242 21.968876435114986 72-73 21.44495828542005 27.157473358177636 29.391233103335026 22.006335253067288 74-75 21.126115206115205 27.825236145236143 28.9945945945946 22.054054054054053 76-77 21.371754969693104 27.45056075835809 28.946076525892543 22.23160774605626 78-79 21.35710722799894 27.45501336997665 29.085442930160767 22.102436471863644 80-81 21.330991165771394 27.734944316014428 28.886053868398076 22.048010649816106 82-83 21.41288257564179 27.546581545542274 28.96832847904213 22.072207399773806 84-85 21.27699153300991 27.47758802132686 28.83278677071007 22.412633674953167 86-87 21.33186841403126 27.517804577377913 28.899594366989362 22.250732641601466 88-89 21.31812703070957 27.3767162771198 28.679209027704992 22.62594766446564 90-91 21.161880271854532 27.406044492115743 28.959486477586676 22.472588758443045 92-93 21.497625786582386 27.517249240221137 28.919373695034217 22.06575127816226 94-95 21.21328843877876 27.360479690454664 28.813307654346865 22.612924216419703 96-97 21.066526793536603 27.358546600550117 28.912378684662666 22.66254792125061 98-99 21.293854342563773 27.58932915883001 28.301376660111977 22.815439838494246 100-101 21.58730435997764 26.882292831190608 28.851924958077134 22.67847785075461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1474.0 1 896.5 2 362.5 3 413.5 4 351.0 5 335.0 6 588.5 7 1280.0 8 2220.5 9 2724.5 10 2476.0 11 2103.0 12 2106.5 13 2232.5 14 2367.0 15 2497.0 16 2563.0 17 2705.0 18 2824.0 19 2917.0 20 3181.0 21 3538.5 22 4154.0 23 4912.5 24 5689.0 25 6873.0 26 8443.5 27 10171.5 28 11534.0 29 13072.5 30 15160.5 31 17193.5 32 19345.5 33 21465.0 34 23980.0 35 26161.0 36 28740.0 37 31771.5 38 33465.0 39 33954.5 40 34328.0 41 36027.5 42 37101.0 43 37868.0 44 39249.5 45 40170.5 46 40690.0 47 43602.0 48 52004.5 49 51578.0 50 42847.5 51 38513.0 52 35713.0 53 34184.0 54 32929.5 55 30273.0 56 26731.0 57 23289.0 58 20830.0 59 18654.5 60 16153.0 61 13438.0 62 10646.0 63 8016.5 64 5875.5 65 4579.0 66 3521.0 67 2607.0 68 1897.0 69 1346.5 70 993.5 71 761.5 72 668.0 73 581.5 74 426.5 75 340.5 76 257.5 77 183.5 78 137.5 79 93.5 80 64.0 81 51.5 82 39.5 83 26.5 84 15.5 85 9.0 86 8.5 87 6.0 88 6.0 89 4.0 90 2.0 91 3.0 92 3.0 93 2.0 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.320092496355253 2 0.2733560272606151 3 0.2682115278640039 4 0.2747511457410521 5 0.2795468655175541 6 0.21118605997614345 7 0.2030769338086037 8 0.20272815418849446 9 0.2144122714621539 10-11 0.21214520393144387 12-13 0.20918057716051536 14-15 0.2091369797080017 16-17 0.19522939235614586 18-19 0.20002511213264787 20-21 0.1884281897640157 22-23 0.19784523950696514 24-25 0.19841200638964265 26-27 0.19880438346226553 28-29 0.2118400217638483 30-31 0.20874460263537883 32-33 0.21314794533925796 34-35 0.20726228924991455 36-37 0.1913056216299169 38-39 0.1794907119987165 40-41 0.1759157208925968 42-43 0.19161080379751252 44-45 0.19570896433379606 46-47 0.1951857949036322 48-49 0.21053209818843865 50-51 0.20652113255718244 52-53 0.22539882949559492 54-55 0.19666810828909645 56-57 0.17670047503784259 58-59 0.1781391909707932 60-61 0.16597550171948353 62-63 0.17242792469150442 64-65 0.1700736622557671 66-67 0.17046603932838997 68-69 0.17321267883675023 70-71 0.17438981005461887 72-73 0.17373584826691407 74-75 0.18908215155172053 76-77 0.18023186869144864 78-79 0.1854635629930872 80-81 0.17853156804341608 82-83 0.1587819220547305 84-85 0.1587819220547305 86-87 0.16179014627817268 88-89 0.16880933613287108 90-91 0.1725587170490454 92-93 0.1700736622557671 94-95 0.1701172597082807 96-97 0.17517456419986469 98-99 0.1856379528031418 100-101 0.16532153993177873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1146856.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.37677470107175 #Duplication Level Percentage of deduplicated Percentage of total 1 79.08254833035258 46.16584106667611 2 12.33336293305667 14.399638984991975 3 3.788002675126071 6.6339413619867535 4 1.6517705559670193 3.857001504142029 5 0.8030460311981205 2.343961861892126 6 0.49891400186691676 1.7474994169317055 7 0.3339811646280788 1.3647720241326438 8 0.2264134997574123 1.0573831891695697 9 0.17789035049416796 0.934619842106346 >10 0.9845517348848091 10.649946838227505 >50 0.07930293099826291 3.141817265675715 >100 0.036478267620823415 3.9990639686742853 >500 0.0020930134674276655 0.8503090205752246 >1k 0.0013455086576320708 1.6074095803094004 >5k 2.9900192391823794E-4 1.2467940745086379 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8052 0.7020933752798956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6234 0.5435730379402471 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4010 0.3496515691595109 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3093 0.2696938412494681 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2531 0.2206903046241202 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1854 0.16165935392063172 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1638 0.14282525443473287 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.13925026332861318 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1503 0.13105394225604608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.3597452513654724E-5 0.0 22-23 0.0 0.0 0.0 8.719490502730945E-5 0.0 24-25 0.0 0.0 0.0 1.743898100546189E-4 0.0 26-27 0.0 0.0 0.0 3.9237707262289247E-4 0.0 28-29 0.0 0.0 0.0 0.0013079235754096417 0.0 30-31 0.0 0.0 0.0 0.0032262114860104494 0.0 32-33 0.0 0.0 0.0 0.006365228066993589 0.0 34-35 0.0 0.0 0.0 0.009547842100490384 0.0 36-37 0.0 0.0 0.0 0.015346303284806462 0.0 38-39 0.0 0.0 0.0 0.0244581708601603 0.0 40-41 0.0 0.0 0.0 0.035880703418737836 0.0 42-43 0.0 0.0 0.0 0.05336328187671338 0.0 44-45 0.0 0.0 0.0 0.07058427561960699 0.0 46-47 0.0 0.0 0.0 0.09879182739594161 0.0 48-49 0.0 0.0 0.0 0.13428015374205654 0.0 50-51 0.0 0.0 0.0 0.16296727749604134 0.0 52-53 0.0 0.0 0.0 0.2017254127806804 0.0 54-55 0.0 0.0 0.0 0.249508220735646 0.0 56-57 0.0 0.0 0.0 0.3179562211820839 0.0 58-59 0.0 0.0 0.0 0.39930906757256357 0.0 60-61 0.0 0.0 0.0 0.4952670605551176 0.0 62-63 0.0 0.0 0.0 0.604827458721932 0.0 64-65 0.0 0.0 0.0 0.7235433219166137 0.0 66-67 0.0 0.0 0.0 0.8580850603737522 0.0 68-69 0.0 0.0 0.0 1.024409341713345 0.0 70-71 0.0 0.0 0.0 1.1865918650641407 0.0 72-73 0.0 0.0 0.0 1.3585838152305085 0.0 74-75 0.0 0.0 0.0 1.5388592813744708 0.0 76-77 0.0 0.0 0.0 1.7513968623785376 0.0 78-79 0.0 0.0 0.0 1.996065765885168 0.0 80-81 0.0 0.0 0.0 2.303558598463974 0.0 82-83 0.0 0.0 0.0 2.617416659109775 0.0 84-85 0.0 0.0 0.0 2.961574949252565 0.0 86-87 0.0 0.0 0.0 3.335466702009668 0.0 88-89 0.0 0.0 0.0 3.801654261738178 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10705 0.0 27.676018 1 GTACATG 6455 0.0 25.932753 1 ACTGCGT 130 9.997981E-6 25.584513 8 GGTATCA 7535 0.0 25.434547 1 TACATGG 6500 0.0 25.081589 2 GAGTACT 6265 0.0 24.26314 12-13 ACATGGG 6640 0.0 23.763279 3 ATCAACG 12520 0.0 23.573477 3 TCAACGC 12505 0.0 23.567867 4 TATCAAC 12645 0.0 23.45525 2 CAACGCA 12595 0.0 23.436174 5 AACGCAG 12760 0.0 23.122002 6 GTACTTT 6680 0.0 22.539976 14-15 AGTACTT 6540 0.0 21.28178 12-13 TAACGCA 135 3.7771664E-4 21.12661 4 ACGCAGA 13985 0.0 21.02963 7 CGCAGAG 14030 0.0 20.895365 8 CATGGGA 4165 0.0 20.88567 4 GTGTAGG 455 0.0 19.858408 1 GCAGAGT 14395 0.0 19.804417 9 >>END_MODULE