FastQCFastQC Report
Thu 26 May 2016
SRR937530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146856
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85580.7462139972237142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74820.6523922794143292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.4456531595945785No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50350.439026346812503No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.16488556540664215No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15340.1337569843118927No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14580.12713017152981718No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13530.11797470650194969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.11413813068074806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC1004.949629E-528.5029322
CACCGTC1955.347829E-1026.7976267
ACCGTAT950.001225818425.0025718
GTACATG68300.023.0957131
GAGTACT72050.023.0436612-13
TACATGG70100.022.4987452
TCTACGC1954.2172542E-721.9253313
GGTATCA108700.021.7677651
GTACTTT75750.021.75751914-15
GTATCAA140600.021.560031
AGTACTT74100.021.41245812-13
ACATGGG70600.021.2628083
TACTTTT81900.019.0798314-15
ACTTTTT88450.018.82184416-17
TCAACGC162300.018.6448614
ATCAACG162500.018.6219163
CAACGCA162650.018.6047425
AGAGTAC125000.018.56490910-11
CTACGCA1801.2323465E-418.4741214
CATGGGA44900.018.4094664
Produced by FastQC (version 0.11.5)