##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937530_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1146856 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.115414664090345 31.0 31.0 34.0 31.0 34.0 2 32.35006400106029 34.0 31.0 34.0 31.0 34.0 3 32.408578757926016 34.0 31.0 34.0 31.0 34.0 4 36.05813633097791 37.0 35.0 37.0 35.0 37.0 5 35.967410904246044 37.0 35.0 37.0 35.0 37.0 6 36.024956053767866 37.0 35.0 37.0 35.0 37.0 7 35.96516127569634 37.0 35.0 37.0 35.0 37.0 8 35.9470099123168 37.0 35.0 37.0 35.0 37.0 9 37.60287603674742 39.0 37.0 39.0 35.0 39.0 10-11 37.523817724282736 39.0 37.0 39.0 35.0 39.0 12-13 37.416858350132884 39.0 37.0 39.0 34.5 39.0 14-15 38.54345358092036 40.0 38.0 41.0 34.0 41.0 16-17 38.514199254309176 40.0 38.0 41.0 34.0 41.0 18-19 38.53903061936285 40.0 38.0 41.0 34.0 41.0 20-21 38.44413335239995 40.0 38.0 41.0 34.0 41.0 22-23 38.28220064245206 40.0 38.0 41.0 34.0 41.0 24-25 38.334323140830236 40.0 38.0 41.0 34.0 41.0 26-27 38.17844960483269 40.0 38.0 41.0 33.5 41.0 28-29 38.02801659493433 40.0 37.0 41.0 33.0 41.0 30-31 38.05959815356069 40.0 37.0 41.0 34.0 41.0 32-33 37.94675007149982 40.0 37.0 41.0 33.0 41.0 34-35 37.77886674525834 40.0 37.0 41.0 33.0 41.0 36-37 37.57272142274182 40.0 36.5 41.0 33.0 41.0 38-39 37.408142347426356 40.0 36.0 41.0 33.0 41.0 40-41 37.20134306312214 40.0 36.0 41.0 32.0 41.0 42-43 36.945004429501175 39.0 36.0 41.0 31.5 41.0 44-45 36.55057827661014 39.0 35.0 41.0 30.5 41.0 46-47 36.0855089915386 39.0 35.0 41.0 29.5 41.0 48-49 35.65427220156672 39.0 35.0 40.0 28.0 41.0 50-51 35.348323154781426 39.0 35.0 40.0 27.0 41.0 52-53 35.08611674002665 38.5 35.0 40.0 26.5 41.0 54-55 34.84787976868935 38.0 34.0 40.0 26.0 41.0 56-57 34.5538816555871 38.0 34.0 40.0 25.0 41.0 58-59 34.26952163131204 38.0 34.0 40.0 24.0 41.0 60-61 33.86885057932295 37.5 33.0 40.0 23.0 41.0 62-63 33.51209131748014 37.0 33.0 40.0 22.0 41.0 64-65 33.102325400922176 36.0 32.5 39.0 21.5 40.5 66-67 32.64479585928835 36.0 32.0 39.0 20.0 40.0 68-69 32.17059552376236 35.0 31.0 38.5 18.5 40.0 70-71 31.719116872562903 35.0 31.0 38.0 17.0 40.0 72-73 31.190736238900087 35.0 30.5 37.0 12.5 39.0 74-75 30.671017983077213 34.0 30.0 36.5 8.0 39.0 76-77 29.49336446772742 33.5 28.5 35.5 7.5 37.5 78-79 30.10468620297579 34.0 30.0 36.0 7.0 38.0 80-81 29.883412564437034 34.0 30.0 35.5 4.5 37.0 82-83 29.508045909861394 34.0 29.0 35.0 2.0 37.0 84-85 29.077705919487713 34.0 29.0 35.0 2.0 36.0 86-87 28.616202034082747 34.0 29.0 35.0 2.0 36.0 88-89 28.181064144059935 33.5 28.0 35.0 2.0 35.5 90-91 27.580111190942894 33.0 26.5 35.0 2.0 35.0 92-93 27.0225111086309 33.0 25.5 35.0 2.0 35.0 94-95 26.44238814637583 32.0 25.0 34.0 2.0 35.0 96-97 25.7703464951136 32.0 23.5 34.0 2.0 35.0 98-99 24.756384410946097 32.0 18.0 34.0 2.0 35.0 100-101 22.048083194402786 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36477193567392163 1101 2 0.36645693095189813 1101 3 0.29443026733907374 1101 4 0.2647376881626968 1101 5 0.25492110114256405 1101 6 0.3053083411045634 1101 7 0.3053415060122049 1101 8 0.3002881079104611 1101 9 0.4456596036279805 1101 10-11 0.384381053561448 1101 12-13 0.38403571462591657 1101 14-15 0.4609061689333416 1101 16-17 0.5298747715512917 1101 18-19 0.493627174901043 1101 20-21 0.4979172668589129 1101 22-23 0.43321508477858117 1101 24-25 0.463382075029962 1101 26-27 0.4813743760792377 1101 28-29 0.4304709340744566 1101 30-31 0.5017544228198005 1101 32-33 0.5218723953646602 1101 34-35 0.5464422387211556 1101 36-37 0.5038710231973695 1101 38-39 0.5563841748459666 1101 40-41 0.6017477835015015 1101 42-43 0.6168992851864346 1101 44-45 0.6400271107054749 1101 46-47 0.7848180911775344 1101 48-49 0.7371184548421326 1101 50-51 0.6601681127032677 1101 52-53 0.625119105071164 1101 54-55 0.7583451985006846 1101 56-57 0.7004185183146205 1101 58-59 0.700731930434344 1101 60-61 0.6355450283970967 1101 62-63 0.5921648785674947 1101 64-65 0.6387615014426657 1101 66-67 0.4600502340698185 1101 68-69 0.4445272946167833 1101 70-71 0.42013128493988816 1101 72-73 0.6997169138670678 1101 74-75 0.8311453202376704 1101 76-77 0.6838413745760086 1101 78-79 0.6798998419100251 1101 80-81 0.6798590996968166 1101 82-83 0.6341729470022486 1101 84-85 0.6228792216455794 1101 86-87 0.39415980729767597 1101 88-89 0.6268708098435916 1101 90-91 0.7623281819018324 1101 92-93 0.8561205704821546 1101 94-95 0.8466430779891816 1101 96-97 1.1025674565116361 1101 98-99 1.1481297673043827 1101 100-101 1.4442478824337321 1102 1 0.25360846999416253 1102 2 0.28999342753873947 1102 3 0.27851267861792905 1102 4 0.18409158937740955 1102 5 0.1673119506015297 1102 6 0.23929394142777483 1102 7 0.12119725387683644 1102 8 0.1974869743837644 1102 9 0.26856225525718713 1102 10-11 0.25272982646352915 1102 12-13 0.2369207001614413 1102 14-15 0.3694858016615399 1102 16-17 0.2989501506957737 1102 18-19 0.35540833449808673 1102 20-21 0.37202895964040295 1102 22-23 0.31095695018085934 1102 24-25 0.31476463266869104 1102 26-27 0.3418089010004408 1102 28-29 0.37990841096750927 1102 30-31 0.3897338562089061 1102 32-33 0.3206304327702867 1102 34-35 0.26850641790527163 1102 36-37 0.38962409046573754 1102 38-39 0.4161226328075287 1102 40-41 0.5079304875001043 1102 42-43 0.461575587846454 1102 44-45 0.46993235793549104 1102 46-47 0.6213231108636919 1102 48-49 0.526666587800932 1102 50-51 0.5786487143033199 1102 52-53 0.6660098230349334 1102 54-55 0.6752938334280927 1102 56-57 0.500214462545415 1102 58-59 0.488336936018122 1102 60-61 0.48594947141013023 1102 62-63 0.4819278346557283 1102 64-65 0.6249128181097845 1102 66-67 0.6040853547214269 1102 68-69 0.6446882333019062 1102 70-71 0.5023031567835297 1102 72-73 0.6939921085371275 1102 74-75 0.6520000294808312 1102 76-77 0.5397917227524154 1102 78-79 0.5969530536325358 1102 80-81 0.5344847290633155 1102 82-83 0.6205779636392528 1102 84-85 0.6938168300338283 1102 86-87 0.5624029076113573 1102 88-89 0.6088619234363826 1102 90-91 0.6001977024589173 1102 92-93 0.5754576536519131 1102 94-95 0.7007572577450212 1102 96-97 0.7550723447026044 1102 98-99 0.6555914050103411 1102 100-101 0.9374701626747672 1103 1 0.25582519510466284 1103 2 0.1699105920214521 1103 3 0.20108218889165386 1103 4 0.11370000649587553 1103 5 0.0057009727833658985 1103 6 0.15606667530660445 1103 7 0.1675498409026588 1103 8 0.1442383003705885 1103 9 0.16298317856745115 1103 10-11 0.08331605671830289 1103 12-13 0.18736472408074434 1103 14-15 0.21043300910587703 1103 16-17 0.198964809846629 1103 18-19 0.145021232338145 1103 20-21 0.08715048978419304 1103 22-23 0.20267007939913384 1103 24-25 0.19091442052588548 1103 26-27 0.07691578205110972 1103 28-29 0.12471195571614402 1103 30-31 0.2790700908639465 1103 32-33 0.1294986155736666 1103 34-35 0.22437332186746772 1103 36-37 0.23701453531439398 1103 38-39 0.24468826381230713 1103 40-41 0.16878638075533559 1103 42-43 0.3359268009854759 1103 44-45 0.4887811846251111 1103 46-47 0.5853203117956483 1103 48-49 0.5604099163786316 1103 50-51 0.5547089128732168 1103 52-53 0.3294653897154589 1103 54-55 0.2660965866328624 1103 56-57 0.23921381331852842 1103 58-59 0.394855430435328 1103 60-61 0.26476953800240466 1103 62-63 0.29961036622821524 1103 64-65 0.43606310131049497 1103 66-67 0.42952742995429816 1103 68-69 0.3834389065416417 1103 70-71 0.49936164604111255 1103 72-73 0.7144710428596035 1103 74-75 0.4632648451552086 1103 76-77 0.5503050746489784 1103 78-79 0.6071023154701116 1103 80-81 0.5415935371762863 1103 82-83 0.6112628653173324 1103 84-85 0.48759865962660953 1103 86-87 0.6052253548698481 1103 88-89 0.760683580659677 1103 90-91 0.8169648040053445 1103 92-93 0.8916926255078508 1103 94-95 0.9278237328453329 1103 96-97 0.9728166810654955 1103 98-99 0.9208631977364874 1103 100-101 1.07995685754668 1104 1 0.0961830243858941 1104 2 0.15877478610160978 1104 3 0.07993897452919185 1104 4 0.07545140440738862 1104 5 -0.026200412279834495 1104 6 0.0194300481466243 1104 7 -0.04431538855619266 1104 8 0.1224157032681461 1104 9 0.1633291062532365 1104 10-11 0.11658612525766188 1104 12-13 0.12161757588034305 1104 14-15 0.1245260308939109 1104 16-17 0.1252808603202098 1104 18-19 0.18704994343442394 1104 20-21 0.13297253388321195 1104 22-23 0.14747688134126236 1104 24-25 0.0788649567739057 1104 26-27 0.22931090299807266 1104 28-29 0.12517073817691227 1104 30-31 0.11856174847668655 1104 32-33 0.08503112931985868 1104 34-35 0.006947101991507054 1104 36-37 0.0609822747776505 1104 38-39 0.0634888775258915 1104 40-41 0.1326399430441043 1104 42-43 0.10651002424407352 1104 44-45 0.04166604420281317 1104 46-47 0.03885434930671039 1104 48-49 0.06855289180754198 1104 50-51 0.07523570874432295 1104 52-53 0.18454468108965472 1104 54-55 0.013785768653640673 1104 56-57 0.12021769486158718 1104 58-59 0.12933624587494563 1104 60-61 0.10821547064718828 1104 62-63 0.04522022531753578 1104 64-65 0.008331181742441629 1104 66-67 -0.10177722233728304 1104 68-69 -0.05020368794605545 1104 70-71 0.14334585538897215 1104 72-73 0.09951019640371328 1104 74-75 -0.028172768394576053 1104 76-77 -0.032811340085554974 1104 78-79 0.2051284839528229 1104 80-81 0.25495548242594523 1104 82-83 0.35951121194228364 1104 84-85 0.39207618409475486 1104 86-87 0.3386309693573324 1104 88-89 0.37128246233046625 1104 90-91 0.3756912130745995 1104 92-93 0.25519845683880504 1104 94-95 0.29432960775535477 1104 96-97 0.5592820725182861 1104 98-99 0.5302474254176133 1104 100-101 0.7565239385150981 1105 1 0.12434799954977649 1105 2 0.12639619429020854 1105 3 0.05416676891785954 1105 4 0.045635233761124994 1105 5 -0.04348843299096927 1105 6 -0.031801129247604365 1105 7 0.011429805149447247 1105 8 0.029039134843003467 1105 9 0.19518911760345503 1105 10-11 0.10500208855195581 1105 12-13 0.13454458758550203 1105 14-15 0.1460609568668545 1105 16-17 0.1316788918802274 1105 18-19 0.15641419106160726 1105 20-21 0.17735929143180584 1105 22-23 0.22171029067691705 1105 24-25 0.09213267544099324 1105 26-27 0.11183057035257349 1105 28-29 0.10277692584849518 1105 30-31 0.005816219879555717 1105 32-33 0.11723866578191178 1105 34-35 0.20506870168990332 1105 36-37 0.1845668569353549 1105 38-39 0.1146837582985043 1105 40-41 0.06029596337021559 1105 42-43 0.0879235470417683 1105 44-45 0.14689476305238713 1105 46-47 0.09336686161447716 1105 48-49 -0.04757644448567078 1105 50-51 0.236223458133189 1105 52-53 0.2620800803224199 1105 54-55 0.308163401913383 1105 56-57 0.3834105141465187 1105 58-59 0.25156000552312463 1105 60-61 0.3390750187642695 1105 62-63 0.2524596967906021 1105 64-65 0.34670172412626954 1105 66-67 0.41812235032514167 1105 68-69 0.32289550209783613 1105 70-71 0.2148820848317463 1105 72-73 0.3423857310770124 1105 74-75 0.27450736612350823 1105 76-77 0.29195177933835126 1105 78-79 0.4686391493440958 1105 80-81 0.5444422963245437 1105 82-83 0.6483587240194346 1105 84-85 0.5093728277601919 1105 86-87 0.5293410395642262 1105 88-89 0.2629939984395868 1105 90-91 0.39078579485128984 1105 92-93 0.5918155314915268 1105 94-95 0.5303783695592799 1105 96-97 0.5224299722273109 1105 98-99 0.9544576730969361 1105 100-101 0.932266565387831 1106 1 2.8837160471795187E-4 1106 2 0.022326296766017606 1106 3 -0.011298310488953689 1106 4 0.017060156645385405 1106 5 0.06574791058815777 1106 6 -0.06054843911685737 1106 7 0.06750846776480302 1106 8 0.04431795069721289 1106 9 -0.042885373867271426 1106 10-11 -0.09821109857490029 1106 12-13 -0.2169610003210849 1106 14-15 -0.062423840239212325 1106 16-17 0.015918629142113616 1106 18-19 0.01213626212560115 1106 20-21 -0.056044795045131934 1106 22-23 0.03766483839959989 1106 24-25 0.10230755338913866 1106 26-27 0.09400743139185153 1106 28-29 0.19847073142508265 1106 30-31 0.17840068233898876 1106 32-33 0.23396096380682252 1106 34-35 0.11829344075191273 1106 36-37 0.11545350149255995 1106 38-39 0.21504796886888045 1106 40-41 0.20944925964220573 1106 42-43 0.04803449498186296 1106 44-45 0.33855950260803525 1106 46-47 0.32174427347663936 1106 48-49 0.2865889000308073 1106 50-51 0.05675827034315262 1106 52-53 0.08627175337850446 1106 54-55 0.26834034558147124 1106 56-57 -0.03891439194370605 1106 58-59 0.022385815969443 1106 60-61 0.031235262830428212 1106 62-63 -0.04039580958711042 1106 64-65 0.11113319239171204 1106 66-67 0.1863842535668212 1106 68-69 0.13273256776680853 1106 70-71 0.15367802228496785 1106 72-73 0.08312419644562041 1106 74-75 -0.030611809820562996 1106 76-77 -0.15418578044009834 1106 78-79 0.11606528912519565 1106 80-81 -0.08967390015386201 1106 82-83 -0.0771610191659704 1106 84-85 -0.008460237387250658 1106 86-87 0.12348823793826114 1106 88-89 -0.27684273993349606 1106 90-91 -0.04989450842859 1106 92-93 0.29108212042989123 1106 94-95 0.3281886527668014 1106 96-97 -0.09410909375607801 1106 98-99 0.3468688082395559 1106 100-101 0.41401579557181023 1107 1 0.22486241436726573 1107 2 0.2198237500300806 1107 3 0.14560081937963076 1107 4 0.0092539363892854 1107 5 0.10817035127001162 1107 6 0.16991238073418913 1107 7 0.13873790504320027 1107 8 0.19966439968695937 1107 9 0.2675377033975863 1107 10-11 0.2684138562866778 1107 12-13 0.19164894636913488 1107 14-15 0.27021945758388455 1107 16-17 0.2130699202019244 1107 18-19 0.26654892140262376 1107 20-21 0.2287162318569571 1107 22-23 0.37649745180710426 1107 24-25 0.32529660141480576 1107 26-27 0.41734029886706736 1107 28-29 0.2154395461285361 1107 30-31 0.0585550456388475 1107 32-33 0.0039880832121781395 1107 34-35 -0.00902437169684589 1107 36-37 -0.011134825610930932 1107 38-39 0.10860652440711505 1107 40-41 0.3819676967178367 1107 42-43 0.49577733160542437 1107 44-45 0.11662792558674084 1107 46-47 0.2179828596160931 1107 48-49 0.11757207339708486 1107 50-51 0.19179020855259665 1107 52-53 -0.018380715117494617 1107 54-55 0.09677112808576993 1107 56-57 0.039017343415494565 1107 58-59 0.4059759502254465 1107 60-61 0.3799098695505734 1107 62-63 0.4531507090390008 1107 64-65 0.45835367550004236 1107 66-67 0.6620793852417535 1107 68-69 0.4730871638880103 1107 70-71 0.2655950499916777 1107 72-73 0.5422960407348327 1107 74-75 0.6066792709891224 1107 76-77 0.4844548078660047 1107 78-79 0.4077721798130689 1107 80-81 0.4273518677243864 1107 82-83 0.4634070099875913 1107 84-85 0.43530314761863664 1107 86-87 0.5212407283269087 1107 88-89 0.7688054316272392 1107 90-91 0.8192436567420778 1107 92-93 0.4273843971835447 1107 94-95 0.7177810861538028 1107 96-97 0.7300655497210897 1107 98-99 1.1307945219113051 1107 100-101 1.1272355564654184 1108 1 0.11898314791762488 1108 2 0.17476575550620765 1108 3 0.12616646459873238 1108 4 0.10167474125498188 1108 5 -0.08329656469243218 1108 6 -0.052256159961743265 1108 7 0.07697253621724087 1108 8 -0.06718154069636739 1108 9 0.033431232915859255 1108 10-11 0.010847224477075201 1108 12-13 0.049530954579630304 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14.0 5 29.0 6 63.0 7 138.0 8 367.0 9 647.0 10 1178.0 11 1926.0 12 2912.0 13 4280.0 14 5546.0 15 7936.0 16 11314.0 17 15933.0 18 14347.0 19 8502.0 20 6823.0 21 6798.0 22 7042.0 23 7816.0 24 9199.0 25 10484.0 26 12790.0 27 15990.0 28 19428.0 29 24123.0 30 30047.0 31 37945.0 32 47775.0 33 62111.0 34 85053.0 35 120180.0 36 174694.0 37 232454.0 38 150632.0 39 10330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52766445090587 20.004290819912892 15.48183640115329 20.98620832802795 2 15.66456468815614 21.499560537678665 39.767067530710044 23.06880724345515 3 19.962401556952226 25.664512371213128 30.445321818955474 23.927764252879175 4 12.764996446804522 17.631086754646404 37.50107903789058 32.102837760658495 5 12.984549367452935 38.68248797484639 33.83439957088844 14.498563086812233 6 30.731844276875215 37.37879908201204 16.901773195588635 14.98758344552411 7 27.760416303354564 34.42350216592144 20.149173043520722 17.666908487203276 8 26.68556470908292 34.81212985762816 19.053394672042522 19.4489107612464 9 26.669695236367946 17.375503114602008 20.58506037375224 35.3697412752778 10-11 25.194619028020952 27.493381906708425 26.23136644879566 21.080632616474954 12-13 26.620326973433517 24.961393277590833 27.429433189660813 20.988846559314837 14-15 22.939235614584568 26.073979645221367 27.10715207488996 23.8796326653041 16-17 21.05207628507851 29.592032478358227 27.622168781433764 21.733722455129502 18-19 21.032152249279772 27.536630579601972 31.06405686502926 20.367160306088994 20-21 22.159451578925342 25.96546558591488 31.377130171529817 20.49795266362996 22-23 21.500473691071456 26.202680485453783 31.270891563764746 21.025954259710016 24-25 21.78094799214025 26.302834140831166 31.492459163338367 20.42375870369022 26-27 21.40736112921628 26.393503588311795 31.7528862792252 20.446249003246724 28-29 20.771064565943202 27.058978430351843 31.627345678527924 20.542611325177027 30-31 21.014931255536876 26.58755763583222 31.52858772156225 20.868923387068648 32-33 21.042484845525504 26.72471522144018 31.37904845944042 20.853751473593896 34-35 21.670331759174648 26.366780136303074 31.27158073899426 20.691307365528015 36-37 21.618407193230883 26.349907922180293 31.37263963392091 20.659045250667912 38-39 21.27252355354426 26.774526858234037 31.394640124514435 20.558309463707268 40-41 21.21391136477288 26.80014282643754 31.24594267846656 20.74000313032302 42-43 21.092910614369238 27.002490686089832 30.70027871963386 21.204319979907073 44-45 21.507451676583635 26.88820566836639 30.373821996833083 21.2305206582169 46-47 21.823446012402602 27.42994761330106 29.648578374268435 21.098028000027902 48-49 21.98405902746291 27.63991294460682 29.36044281060569 21.01558521732458 50-51 21.688904273945464 27.846259687353946 29.388345180214433 21.076490858486157 52-53 21.918454417317612 28.174865970473178 28.781917163932157 21.12476244827705 54-55 21.432115278640037 27.926522597431585 28.987030629826236 21.654331494102134 56-57 21.950576183932423 28.126416917206694 28.69074234254344 21.232264556317446 58-59 22.204356955014404 27.59840816981382 28.860903199704236 21.33633167546754 60-61 21.806181421207196 27.7017777297237 29.587498343296804 20.9045425057723 62-63 21.60510997021422 27.630147115243766 29.549830144325007 21.214912770217012 64-65 21.483080700628502 27.894216885118965 29.267449444394067 21.355252969858466 66-67 21.58993805673947 27.904941858437326 28.807321930564957 21.697798154258248 68-69 21.546742375129213 27.85028279499902 29.026498979165204 21.57647585070656 70-71 21.77528981431829 28.051758940755374 28.742822763121755 21.430128481804587 72-73 21.785647021073267 27.647106524271575 29.021516214764535 21.545730239890624 74-75 21.565567080784337 28.459370662053473 28.327270380937104 21.64779187622509 76-77 21.728011188850214 27.854456008426514 28.622686719169625 21.794846083553647 78-79 21.67312199613552 28.012278786525947 28.603198657896023 21.711400559442513 80-81 21.641993415040773 28.32129752994273 28.462422483729434 21.574286571287065 82-83 21.71931596028719 28.07285099674408 28.488168296370702 21.719664746598024 84-85 21.471742418264263 28.07691318361619 28.44365639317732 22.007688004942228 86-87 21.572324685923952 28.049728998235175 28.324436546523714 22.05350976931716 88-89 21.455701500449926 27.944920722392347 28.315499068758417 22.28387870839931 90-91 21.49035602227559 27.875490744955638 28.47977633715465 22.15437689561412 92-93 21.683423000793493 28.05664309444294 28.492017125467594 21.767916779295973 94-95 21.29818390451809 28.029368987911297 28.335684689272238 22.336762418298374 96-97 21.18068876999379 27.99619132654841 28.49102241257839 22.332097490879413 98-99 21.41549198753442 28.181138050884257 27.852713286444992 22.55065667513633 100-101 21.23118892163699 27.86030211100951 28.196940342211075 22.711568625142426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 516.0 1 357.5 2 278.5 3 382.0 4 355.5 5 330.0 6 581.5 7 1352.5 8 2235.5 9 2672.5 10 2504.0 11 2211.0 12 2319.0 13 2546.0 14 2745.0 15 2888.0 16 2900.0 17 2930.0 18 3035.5 19 3271.5 20 3560.5 21 3881.0 22 4422.0 23 5169.0 24 5954.5 25 6981.0 26 8613.0 27 10430.5 28 11867.5 29 13498.5 30 15567.5 31 17386.0 32 19418.5 33 21658.0 34 24129.0 35 26257.0 36 29141.0 37 31978.5 38 33433.5 39 34084.5 40 34140.5 41 35575.0 42 37116.0 43 38228.0 44 39422.0 45 40433.5 46 40117.5 47 42891.0 48 53057.5 49 52205.0 50 42487.5 51 38239.5 52 35310.5 53 33626.5 54 32032.5 55 29491.0 56 26045.5 57 22816.0 58 20694.0 59 18512.0 60 15876.5 61 12956.5 62 10270.0 63 8020.5 64 5966.5 65 4479.0 66 3317.5 67 2535.5 68 1789.5 69 1189.5 70 888.0 71 715.0 72 626.0 73 546.0 74 454.0 75 363.0 76 264.0 77 178.0 78 117.5 79 82.5 80 64.5 81 46.5 82 29.5 83 22.0 84 15.5 85 6.5 86 2.5 87 2.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019357268916062695 2 0.0 3 0.0 4 8.719490502730945E-5 5 0.026245666413220143 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003051821675955831 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010027414078140587 24-25 4.3597452513654724E-5 26-27 6.539617877048209E-4 28-29 0.0011335337653550228 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.719490502730945E-5 40-41 8.283515977594398E-4 42-43 0.01604386252502494 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.3597452513654724E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.3597452513654724E-5 70-71 8.719490502730945E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001918287910600808 84-85 3.923770726228925E-4 86-87 0.0 88-89 0.0 90-91 0.011335337653550229 92-93 0.0022670675307100456 94-95 0.0 96-97 0.0 98-99 0.00156950829049157 100-101 0.01957525617863097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1146856.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.170668413512445 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6367448749143 43.609078530732724 2 13.046723989859958 14.656864142340833 3 4.0704263888864975 6.8591571297526315 4 1.7789988755719441 3.9971022379105268 5 0.9450372090127939 2.6541685852944448 6 0.5449948477862131 1.8367634929243235 7 0.35272360131274616 1.3868904315670758 8 0.24538908157134792 1.1026934986592434 9 0.18093843170784546 0.9147089385650118 >10 1.065002871760033 11.022803385587315 >50 0.08864924627384914 3.4076123978933373 >100 0.039716327741391 4.211854270397323 >500 0.002482268587266672 0.933924161846108 >1k 0.0015514178670416698 1.1244213362228546 >5k 6.20567146816668E-4 2.28195746030626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8558 0.7462139972237142 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7482 0.6523922794143292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 0.4456531595945785 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5035 0.439026346812503 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1891 0.16488556540664215 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1534 0.1337569843118927 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1458 0.12713017152981718 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1353 0.11797470650194969 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.11413813068074806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.3597452513654724E-5 0.0 22-23 0.0 0.0 0.0 8.719490502730945E-5 0.0 24-25 0.0 0.0 0.0 1.743898100546189E-4 0.0 26-27 0.0 0.0 0.0 3.9237707262289247E-4 0.0 28-29 0.0 0.0 0.0 0.0013079235754096417 0.0 30-31 0.0 0.0 0.0 0.0031390165809831398 0.0 32-33 0.0 0.0 0.0 0.0062780331619662795 0.0 34-35 0.0 0.0 0.0 0.009460647195463075 0.0 36-37 0.0 0.0 0.0 0.015259108379779153 0.0 38-39 0.0 0.0 0.0 0.024501768312673956 0.0 40-41 0.0 0.0 0.0 0.036055093228792454 0.0 42-43 0.0 0.0 0.0 0.053581269139281656 0.0 44-45 0.0 0.0 0.0 0.07088945778720257 0.0 46-47 0.0 0.0 0.0 0.09940219173113277 0.0 48-49 0.0 0.0 0.0 0.135108505339816 0.0 50-51 0.0 0.0 0.0 0.1638828239988281 0.0 52-53 0.0 0.0 0.0 0.20290254399854907 0.0 54-55 0.0 0.0 0.0 0.2503365723334054 0.0 56-57 0.0 0.0 0.0 0.318697377874816 0.0 58-59 0.0 0.0 0.0 0.40018101662283667 0.0 60-61 0.0 0.0 0.0 0.4969237637506365 0.0 62-63 0.0 0.0 0.0 0.6062225772023688 0.0 64-65 0.0 0.0 0.0 0.724153686251805 0.0 66-67 0.0 0.0 0.0 0.8580850603737522 0.0 68-69 0.0 0.0 0.0 1.0239297697356948 0.0 70-71 0.0 0.0 0.0 1.186417475254086 0.0 72-73 0.0 0.0 0.0 1.3580170483478309 0.0 74-75 0.0 0.0 0.0 1.538684891564416 0.0 76-77 0.0 0.0 0.0 1.7506121082332917 0.0 78-79 0.0 0.0 0.0 1.996065765885168 0.0 80-81 0.0 0.0 0.0 2.3051717042069795 0.0 82-83 0.0 0.0 0.0 2.620686468048299 0.0 84-85 0.0 0.0 0.0 2.9672426180793403 0.0 86-87 0.0 0.0 0.0 3.340829188668848 0.0 88-89 0.0 0.0 0.0 3.808368269425281 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAAGC 100 4.949629E-5 28.502932 2 CACCGTC 195 5.347829E-10 26.797626 7 ACCGTAT 95 0.0012258184 25.002571 8 GTACATG 6830 0.0 23.095713 1 GAGTACT 7205 0.0 23.04366 12-13 TACATGG 7010 0.0 22.498745 2 TCTACGC 195 4.2172542E-7 21.925331 3 GGTATCA 10870 0.0 21.767765 1 GTACTTT 7575 0.0 21.757519 14-15 GTATCAA 14060 0.0 21.56003 1 AGTACTT 7410 0.0 21.412458 12-13 ACATGGG 7060 0.0 21.262808 3 TACTTTT 8190 0.0 19.07983 14-15 ACTTTTT 8845 0.0 18.821844 16-17 TCAACGC 16230 0.0 18.644861 4 ATCAACG 16250 0.0 18.621916 3 CAACGCA 16265 0.0 18.604742 5 AGAGTAC 12500 0.0 18.564909 10-11 CTACGCA 180 1.2323465E-4 18.474121 4 CATGGGA 4490 0.0 18.409466 4 >>END_MODULE