FastQCFastQC Report
Thu 26 May 2016
SRR937529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040179
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76140.7319893979786172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59940.5762469728767837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.3753200170355295No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28470.2737028915215554No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22420.21553982535698182No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17260.16593297884306452No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14980.14401367456947312No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14850.14276388967668063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.1417063793827793No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10930.10507806829401478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG650.00584024829.2344196
GTATACG700.00837820327.1514844
GTATCAA99650.026.9147051
GTACATG59750.026.4233551
TACATGG59850.025.7336272
GGTATCA71350.025.5932251
ACATGGG61900.024.0296863
GAGTACT57450.023.89306412-13
ACTATAC3300.023.0409553
TATCAAC117400.022.8365842
ATCAACG117050.022.735843
CAACGCA117700.022.646295
TCAACGC117750.022.6377644
AACGCAG119600.022.3627416
GTACTTT61300.022.35369914-15
AGTACTT59400.020.94975512-13
CATGGGG21000.020.8161374
CATGGGA38850.020.6693734
CGCAGAG129150.020.5987788
ACGCAGA129150.020.5619957
Produced by FastQC (version 0.11.5)