FastQCFastQC Report
Thu 26 May 2016
SRR937529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040179
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76880.7391035581375898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67770.6515224783426699No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46590.44790367811693954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46410.4461732067269191No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17480.16804799943086718No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14600.14036045719054124No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13330.1281510201609531No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.1174797799224941No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11970.11507634743635471No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10710.1029630477062121No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10710.1029630477062121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG851.6324444E-533.528242
CGTATAG650.005844816629.2297483
GTACATG63950.023.925491
TACATGG65000.023.2376522
GAGTACT63650.022.87673412-13
AGTACTT64950.022.38227512-13
ACATGGG64450.022.183093
GTACTTT66850.021.8119514-15
ATGGGCG1100.002877767721.5901555
ATGGGAT16400.019.9840585
AGTACAT50750.019.7480812
CATGGGA42150.019.7205454
GTATCAA133700.019.6179281
TACTTTT74400.019.37507214-15
CTATTCC3203.965397E-1019.2962024
GGTATCA105400.019.1148431
GCACCGT2001.2854618E-518.9984236
ACTTTTT77900.018.99232516-17
AGAGTAC110600.018.9858110-11
CATGGGG22050.018.3100194
Produced by FastQC (version 0.11.5)