##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1040179 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16405926287687 33.0 31.0 34.0 31.0 34.0 2 32.38207750781356 34.0 31.0 34.0 31.0 34.0 3 32.43015288714731 34.0 31.0 34.0 31.0 34.0 4 36.06092028391267 37.0 35.0 37.0 35.0 37.0 5 35.980651407113584 37.0 35.0 37.0 35.0 37.0 6 36.03923843876871 37.0 35.0 37.0 35.0 37.0 7 35.98811358429655 37.0 35.0 37.0 35.0 37.0 8 35.962503569097244 37.0 35.0 37.0 35.0 37.0 9 37.63039342267052 39.0 37.0 39.0 35.0 39.0 10-11 37.54198267798138 39.0 37.0 39.0 35.0 39.0 12-13 37.43086286110372 39.0 37.0 39.0 34.5 39.0 14-15 38.572660090234464 40.0 38.0 41.0 34.0 41.0 16-17 38.536971521247786 40.0 38.0 41.0 34.0 41.0 18-19 38.5520203734165 40.0 38.0 41.0 34.0 41.0 20-21 38.46737628811964 40.0 38.0 41.0 34.0 41.0 22-23 38.409743419161515 40.0 38.0 41.0 34.0 41.0 24-25 38.40526245963435 40.0 38.0 41.0 34.0 41.0 26-27 38.19771645072627 40.0 38.0 41.0 34.0 41.0 28-29 38.164380842143515 40.0 37.5 41.0 33.5 41.0 30-31 38.15504350693486 40.0 37.5 41.0 34.0 41.0 32-33 37.995012877591265 40.0 37.0 41.0 33.5 41.0 34-35 37.79818233207938 40.0 37.0 41.0 33.0 41.0 36-37 37.59174046005543 40.0 36.5 41.0 33.0 41.0 38-39 37.43282165857992 40.0 36.0 41.0 33.0 41.0 40-41 37.21931850191169 40.0 36.0 41.0 32.0 41.0 42-43 36.94768592713369 39.5 36.0 41.0 31.5 41.0 44-45 36.56736052160253 39.0 35.5 41.0 30.5 41.0 46-47 36.10354419768136 39.0 35.0 41.0 29.5 41.0 48-49 35.65122781751987 39.0 35.0 40.0 28.0 41.0 50-51 35.34528287919676 39.0 35.0 40.0 27.0 41.0 52-53 35.071747747262734 39.0 35.0 40.0 26.0 41.0 54-55 34.831546301165474 38.0 34.5 40.0 26.0 41.0 56-57 34.53731473140681 38.0 34.0 40.0 25.0 41.0 58-59 34.261408853668456 38.0 34.0 40.0 24.0 41.0 60-61 33.8785314835235 37.5 33.5 40.0 23.0 41.0 62-63 33.460874522558136 37.0 33.0 40.0 22.0 41.0 64-65 33.06248491846115 36.0 32.5 39.0 20.5 40.5 66-67 32.62683586190454 36.0 32.0 39.0 19.5 40.0 68-69 32.12985072761515 35.0 31.0 38.5 18.0 40.0 70-71 31.679823376553458 35.0 31.0 38.0 14.5 40.0 72-73 31.178709625939383 35.0 30.5 37.0 11.5 39.5 74-75 30.683629452238506 34.0 30.0 37.0 7.5 39.0 76-77 29.481207561390875 33.5 28.5 35.5 7.0 37.5 78-79 30.08950286441084 34.0 30.0 36.0 7.0 38.0 80-81 29.908982973122896 34.0 30.0 35.5 2.0 37.0 82-83 29.500817647731786 34.0 29.5 35.0 2.0 37.0 84-85 29.06782198063987 34.0 29.0 35.0 2.0 36.0 86-87 28.69550000528755 34.0 29.0 35.0 2.0 36.0 88-89 28.24814430977745 34.0 28.5 35.0 2.0 35.5 90-91 27.69709011622038 33.0 27.0 35.0 2.0 35.0 92-93 27.18534454166062 33.0 26.0 35.0 2.0 35.0 94-95 26.613761669866435 32.5 25.0 34.5 2.0 35.0 96-97 26.008583618780996 32.0 24.5 34.0 2.0 35.0 98-99 25.106008677352648 32.0 20.5 34.0 2.0 35.0 100-101 22.59822299815705 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3078334654650874 1101 2 0.2641856390215551 1101 3 0.29461709439866723 1101 4 0.19894076635891622 1101 5 0.2038546182783918 1101 6 0.19213190591275975 1101 7 0.15640282583141385 1101 8 0.15997857523399972 1101 9 0.14829608377776538 1101 10-11 0.20151175809820643 1101 12-13 0.26073533245858016 1101 14-15 0.3983321531702515 1101 16-17 0.4559554023921564 1101 18-19 0.43302980868151053 1101 20-21 0.3213458908879545 1101 22-23 0.2428245615326574 1101 24-25 0.27778540385605055 1101 26-27 0.17304055830777543 1101 28-29 0.3242932179101743 1101 30-31 0.4714393927987075 1101 32-33 0.5290726935547809 1101 34-35 0.4912556453263974 1101 36-37 0.42332754029491326 1101 38-39 0.5036647599103574 1101 40-41 0.5282038907795297 1101 42-43 0.5589025546823265 1101 44-45 0.5521959057232593 1101 46-47 0.791618074200251 1101 48-49 0.8145750539035177 1101 50-51 0.9574364134815809 1101 52-53 0.951154242768915 1101 54-55 0.9745262349335633 1101 56-57 0.9999606345938972 1101 58-59 1.0081654500107362 1101 60-61 1.005091219154437 1101 62-63 1.1163841582614324 1101 64-65 1.1571423723513234 1101 66-67 0.9805592762494086 1101 68-69 1.1230146356313782 1101 70-71 1.1005259227322632 1101 72-73 1.1262609986303218 1101 74-75 1.0612156813233717 1101 76-77 0.9076216285350966 1101 78-79 1.076054330837028 1101 80-81 1.1794531428687307 1101 82-83 1.2959315887836276 1101 84-85 1.321635599531266 1101 86-87 1.3238821267098508 1101 88-89 1.5605023745931987 1101 90-91 1.515130209655137 1101 92-93 1.7660426350593994 1101 94-95 1.828848991170947 1101 96-97 1.6940641037699962 1101 98-99 1.7655527944943756 1101 100-101 1.9757360140773095 1102 1 0.10449124936931042 1102 2 0.17270598826406314 1102 3 0.18546644607977214 1102 4 0.14331029629154557 1102 5 0.061111344143007784 1102 6 0.10390687125420328 1102 7 0.0653830092915868 1102 8 0.07741044847969647 1102 9 0.16651819843642812 1102 10-11 0.16248319734248184 1102 12-13 0.09316074750631032 1102 14-15 0.2795426115050077 1102 16-17 0.20892735724000744 1102 18-19 0.3160631624460706 1102 20-21 0.2631018893832362 1102 22-23 0.24662990013426622 1102 24-25 0.29095276194011177 1102 26-27 0.23813945686276838 1102 28-29 0.31042295534694375 1102 30-31 0.304003705138733 1102 32-33 0.25607138087014647 1102 34-35 0.15602742497426192 1102 36-37 0.3076927822997746 1102 38-39 0.26628060630257977 1102 40-41 0.40660957083697724 1102 42-43 0.3404623607522481 1102 44-45 0.6151253232753575 1102 46-47 0.4425995624531609 1102 48-49 0.6482246328710985 1102 50-51 0.6006549110232555 1102 52-53 0.6837044812355728 1102 54-55 0.7504144015682286 1102 56-57 0.7822162424405406 1102 58-59 0.761625255291456 1102 60-61 0.7799822452844936 1102 62-63 0.7042932541926348 1102 64-65 0.7334948452755654 1102 66-67 0.646274400007421 1102 68-69 0.6563569569523651 1102 70-71 0.7547584757328885 1102 72-73 0.7898274233666669 1102 74-75 0.8478645736963841 1102 76-77 0.7543263691943842 1102 78-79 0.5689065130765876 1102 80-81 0.7483293638101287 1102 82-83 0.7935245618433449 1102 84-85 0.8318400784428164 1102 86-87 0.7631194279985962 1102 88-89 0.7437376292513846 1102 90-91 0.795354839196591 1102 92-93 0.9841321659390552 1102 94-95 1.1101890080595957 1102 96-97 1.2105052815558999 1102 98-99 1.1225819708176452 1102 100-101 1.4521708257373156 1103 1 0.12717526774348897 1103 2 0.1578017464717476 1103 3 0.1538275159771274 1103 4 0.08321442441976501 1103 5 0.015016766526350978 1103 6 0.06686783597438506 1103 7 0.15854058257840364 1103 8 0.15148366937145852 1103 9 0.12493108400396835 1103 10-11 0.044347409673065386 1103 12-13 0.1395987422292677 1103 14-15 0.2501045213042161 1103 16-17 0.13146906817252813 1103 18-19 0.2624784243508671 1103 20-21 0.15816856473888663 1103 22-23 0.26102341127865003 1103 24-25 0.024215269915536908 1103 26-27 0.04698449330347643 1103 28-29 0.05242244310077382 1103 30-31 0.22024009007505896 1103 32-33 0.29262923816563813 1103 34-35 0.42323927513771054 1103 36-37 0.3683607634285053 1103 38-39 0.231405432207076 1103 40-41 0.3218782954804098 1103 42-43 0.28418105576724173 1103 44-45 0.44288770167371894 1103 46-47 0.29701586703655636 1103 48-49 0.4660618628620554 1103 50-51 0.36926998227548324 1103 52-53 0.30483115001386807 1103 54-55 0.46307294403085564 1103 56-57 0.5484401243268806 1103 58-59 0.44845128406515045 1103 60-61 0.5280867071304911 1103 62-63 0.2531945770911008 1103 64-65 0.43561339237569285 1103 66-67 0.5974202098186012 1103 68-69 0.634448885437962 1103 70-71 0.5633216252284505 1103 72-73 0.6712101546086835 1103 74-75 0.7159517889110063 1103 76-77 0.7435523773569805 1103 78-79 0.7111560618301489 1103 80-81 0.7137371375246246 1103 82-83 0.8231772498475358 1103 84-85 0.7930015132776767 1103 86-87 0.8977368119361948 1103 88-89 1.071131887547729 1103 90-91 1.316827501467234 1103 92-93 1.1598548801162991 1103 94-95 1.278582358558733 1103 96-97 1.2990847683701077 1103 98-99 1.4636947271112248 1103 100-101 1.984155900884435 1104 1 0.16763341123106557 1104 2 0.21543890036907243 1104 3 0.16206111543711899 1104 4 0.14085666827823928 1104 5 0.12584597209696113 1104 6 0.12148155294983098 1104 7 0.20767436907489412 1104 8 0.1680695035397406 1104 9 0.2292553192275193 1104 10-11 0.21287915556496984 1104 12-13 0.22105750160213233 1104 14-15 0.33844569122003776 1104 16-17 0.23929284745302226 1104 18-19 0.2321275733289454 1104 20-21 0.16131337765736475 1104 22-23 0.16862945768264836 1104 24-25 0.10023795078241449 1104 26-27 0.13306709084376678 1104 28-29 0.19262992557858638 1104 30-31 0.3447074550060947 1104 32-33 0.3792178953046772 1104 34-35 0.4486131229956456 1104 36-37 0.360108370265813 1104 38-39 0.3775149204036481 1104 40-41 0.4857562507412183 1104 42-43 0.463666789584984 1104 44-45 0.40720687825895396 1104 46-47 0.3761489057002976 1104 48-49 0.2531599468049066 1104 50-51 0.20614886508382568 1104 52-53 0.4133156454049143 1104 54-55 0.5414186048585563 1104 56-57 0.47020565957348737 1104 58-59 0.3705100793809706 1104 60-61 0.2993368055037777 1104 62-63 0.4664845048019046 1104 64-65 0.4810724226911063 1104 66-67 0.5209386493774701 1104 68-69 0.5425999492202607 1104 70-71 0.6568300845481083 1104 72-73 0.4212803373422034 1104 74-75 0.5998562354619104 1104 76-77 0.549560841570834 1104 78-79 0.5393469682621763 1104 80-81 0.5712990774576028 1104 82-83 0.45815792952962653 1104 84-85 0.34231521032397083 1104 86-87 0.6064602614263563 1104 88-89 0.5795637997247525 1104 90-91 0.43677478513115986 1104 92-93 0.7183030997457749 1104 94-95 0.887616961289222 1104 96-97 0.9891542933240345 1104 98-99 1.1182329952010477 1104 100-101 1.4195687965750778 1105 1 0.08035296629192601 1105 2 0.05660374154973624 1105 3 0.06798572453577378 1105 4 -0.0057941261261404975 1105 5 0.0873785949296888 1105 6 0.018341154349734268 1105 7 0.0010125906898892367 1105 8 0.10410920998494788 1105 9 0.11788045191153884 1105 10-11 0.06799142485888865 1105 12-13 -0.0027056429813967497 1105 14-15 -0.03436374023294775 1105 16-17 0.16658962183185366 1105 18-19 0.13174342706967934 1105 20-21 0.23703133670012733 1105 22-23 0.09471793558076769 1105 24-25 0.08756329288886633 1105 26-27 -0.012283092408175378 1105 28-29 -0.015597685475960077 1105 30-31 0.021201970928466096 1105 32-33 0.04498368800050656 1105 34-35 0.0033434146924236074 1105 36-37 -0.03263282452635252 1105 38-39 0.017878353008960346 1105 40-41 0.008732639871887216 1105 42-43 0.1978532863223208 1105 44-45 0.2288499504677901 1105 46-47 0.4501058524819115 1105 48-49 0.5983177638647774 1105 50-51 0.3868508785987004 1105 52-53 0.4163500140106535 1105 54-55 0.4735866632787946 1105 56-57 0.5281426405965277 1105 58-59 0.5658001555244567 1105 60-61 0.5449949387780251 1105 62-63 0.4939295101678809 1105 64-65 0.6994098090781264 1105 66-67 0.4574358263068916 1105 68-69 0.6573066435285781 1105 70-71 0.4512977688448281 1105 72-73 0.7305300916093387 1105 74-75 0.7311420586831225 1105 76-77 0.6977971038289077 1105 78-79 0.7291142825382408 1105 80-81 0.8534496400495861 1105 82-83 0.9192559407492276 1105 84-85 0.9003075801500273 1105 86-87 1.075332633315572 1105 88-89 1.0408039462325362 1105 90-91 1.0065251135076352 1105 92-93 0.9579411441752157 1105 94-95 1.2913679261973385 1105 96-97 1.361451451614684 1105 98-99 1.5190386824881728 1105 100-101 1.5663022345903173 1106 1 -0.3754006749085512 1106 2 -0.3564110860869647 1106 3 -0.36174351808701743 1106 4 -0.35792139823899305 1106 5 -0.31734379521475375 1106 6 -0.319062676279529 1106 7 -0.23938361397580366 1106 8 -0.2676011310619657 1106 9 -0.20792673248958948 1106 10-11 -0.5220163475412036 1106 12-13 -0.6573444892117806 1106 14-15 -0.49887306523567077 1106 16-17 -0.40921709322145006 1106 18-19 -0.534872706480428 1106 20-21 -0.37677753359841404 1106 22-23 -0.39106035576784137 1106 24-25 -0.5007430936209545 1106 26-27 -0.48532985447135957 1106 28-29 -0.5983357293092979 1106 30-31 -0.5743952648005504 1106 32-33 -0.6101869293233122 1106 34-35 -0.9447828548934609 1106 36-37 -0.7008893287486089 1106 38-39 -0.7920376525030619 1106 40-41 -0.7449052751724068 1106 42-43 -0.8727887251898494 1106 44-45 -0.939814417020294 1106 46-47 -0.606530819729322 1106 48-49 -0.6397072955546577 1106 50-51 -0.7448584486568919 1106 52-53 -0.9366202068081151 1106 54-55 -0.7902727088464516 1106 56-57 -1.3667525446456708 1106 58-59 -1.2057011379981688 1106 60-61 -1.33850354241239 1106 62-63 -1.4749635235838596 1106 64-65 -1.667271939298871 1106 66-67 -1.5855883507386217 1106 68-69 -1.3378440546732584 1106 70-71 -1.3821292776055891 1106 72-73 -1.3022754444422056 1106 74-75 -1.5416982842036298 1106 76-77 -1.241066586296391 1106 78-79 -1.3679754792958967 1106 80-81 -1.549668988553595 1106 82-83 -1.603492409962488 1106 84-85 -1.435392987197634 1106 86-87 -1.075945682439123 1106 88-89 -1.0544600525358945 1106 90-91 -1.5474658449757293 1106 92-93 -1.526117456717671 1106 94-95 -1.1641496330437455 1106 96-97 -1.533466731671279 1106 98-99 -1.6106853034389026 1106 100-101 -0.9719514910927707 1107 1 0.09717797179731491 1107 2 0.0887067621558657 1107 3 0.016983470297539327 1107 4 0.09804155114589008 1107 5 0.1512949752097228 1107 6 0.09596662916065668 1107 7 0.009023497292055538 1107 8 -0.037587070019405644 1107 9 0.20641344264696215 1107 10-11 0.05039338102962887 1107 12-13 0.14013009185732272 1107 14-15 0.1268787132773852 1107 16-17 0.13579738547584697 1107 18-19 0.11188246431715498 1107 20-21 0.16533888533712826 1107 22-23 0.07775997844875349 1107 24-25 0.08615519353059398 1107 26-27 0.03137575463166087 1107 28-29 0.009350161849880578 1107 30-31 0.11202666392899374 1107 32-33 0.18085750913990495 1107 34-35 0.044967782029999626 1107 36-37 0.0295574689997693 1107 38-39 0.06673008042066186 1107 40-41 -0.025449177645377574 1107 42-43 -0.07105348580407878 1107 44-45 0.2115488776258303 1107 46-47 0.16041842129922657 1107 48-49 0.13713050127024218 1107 50-51 -0.010462493410919649 1107 52-53 0.05783658681011872 1107 54-55 -0.14340848375839244 1107 56-57 0.20402485002411908 1107 58-59 0.2771790786254229 1107 60-61 0.23755138382514218 1107 62-63 0.46097578190272515 1107 64-65 0.4749244141112783 1107 66-67 0.6071987520833346 1107 68-69 0.5302930660150338 1107 70-71 0.1664016218834803 1107 72-73 0.28532763506533243 1107 74-75 0.5001356377134982 1107 76-77 0.452770454109384 1107 78-79 0.652482061460244 1107 80-81 0.6022999054502769 1107 82-83 0.7144263701144524 1107 84-85 0.8414767138079888 1107 86-87 1.0493298536671318 1107 88-89 0.8947769154763918 1107 90-91 0.9354741194772096 1107 92-93 1.0744775852566306 1107 94-95 1.0033316537040697 1107 96-97 0.9772559734319977 1107 98-99 1.09014861383762 1107 100-101 1.6043401061581335 1108 1 0.17841801666138224 1108 2 0.1739448141520512 1108 3 0.02536480507725969 1108 4 0.07034387804003472 1108 5 0.15265832263875012 1108 6 0.11155493014260998 1108 7 0.11569469617879946 1108 8 0.13668701803492667 1108 9 0.24266424948848453 1108 10-11 0.12893618228522996 1108 12-13 0.20599597086668098 1108 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60-61 0.30943752602821206 2316 62-63 0.9411283835295521 2316 64-65 0.3556997376386377 2316 66-67 0.38428268546102373 2316 68-69 0.5661268876603316 2316 70-71 0.5565363959854004 2316 72-73 0.6792166177542605 2316 74-75 0.34279034984118084 2316 76-77 0.7505670912124849 2316 78-79 0.595457569718512 2316 80-81 0.4649261630604933 2316 82-83 0.6591386964326453 2316 84-85 0.5821507829529615 2316 86-87 0.6657339078151345 2316 88-89 1.0015483954092517 2316 90-91 0.6494131086217863 2316 92-93 1.2223827269333079 2316 94-95 1.1662945982748703 2316 96-97 1.2156966360796098 2316 98-99 1.4786098849053815 2316 100-101 1.4739632948518064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 15.0 5 51.0 6 64.0 7 151.0 8 352.0 9 669.0 10 1148.0 11 1844.0 12 2921.0 13 4101.0 14 5333.0 15 7519.0 16 10487.0 17 14942.0 18 13371.0 19 7857.0 20 6293.0 21 6230.0 22 6474.0 23 7005.0 24 7787.0 25 9385.0 26 11099.0 27 13625.0 28 16698.0 29 20528.0 30 25830.0 31 32010.0 32 40987.0 33 54094.0 34 74178.0 35 106520.0 36 157875.0 37 218111.0 38 145096.0 39 9519.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51750867980419 20.01336834107543 15.54459159237139 20.924531386748992 2 15.725466482211234 21.567537894920008 39.71460681286586 22.992388810002893 3 19.958584051398844 25.654815180848683 30.539839777576745 23.84676099017573 4 12.790779279335576 17.71060557846294 37.45739915918318 32.041215983018304 5 13.006236070756232 38.76372733583443 33.716509723309215 14.513526870100128 6 30.629343603360574 37.44893907683197 16.856041123691213 15.065676196116245 7 27.69196455610044 34.51627075724467 20.14057195924932 17.651192727405572 8 26.69492462355037 34.73873246816173 19.101904576039317 19.464438332248584 9 26.72049714520289 17.295004032959714 20.599243014904165 35.38525580693323 10-11 25.17691666530472 27.48709597098192 26.280909343487995 21.055078020225366 12-13 26.648828801198338 25.03659470469215 27.406393269170216 20.908183224939297 14-15 23.030843729781125 26.10718924338984 27.150663491572125 23.71130353525691 16-17 21.084544102505433 29.633601524353022 27.63846414895898 21.643390224182568 18-19 21.01378705011349 27.585059879116958 31.097147702462753 20.3040053683068 20-21 22.17437241389919 26.048524480472533 31.37598829428866 20.401114811339617 22-23 21.526083992928005 26.19363962624945 31.317808142793552 20.962468238028993 24-25 21.75519790343777 26.314461260994506 31.540148378307965 20.390192457259758 26-27 21.412516283474258 26.446813726668367 31.777362245412988 20.36330774444439 28-29 20.763556340905573 27.14517684315571 31.647132794120896 20.444134021817817 30-31 21.04969433145641 26.61248688927579 31.49597328921272 20.84184549005508 32-33 20.971967324854663 26.791542609493174 31.344076356088713 20.89241370956345 34-35 21.719338690744575 26.43059511872476 31.237412022353844 20.61265416817682 36-37 21.654284965787088 26.416837510905587 31.36733874747339 20.56153877583393 38-39 21.283163763159997 26.82115289772241 31.466122657734868 20.429560681382725 40-41 21.225965016514024 26.88615826256409 31.268635659428952 20.61924106149293 42-43 21.124279331236696 27.055026855444098 30.680307466106754 21.140386347212452 44-45 21.5274486410512 26.964253267947154 30.30146734360144 21.206830747400208 46-47 21.788749820944282 27.548479636677918 29.719692476006532 20.943078066371267 48-49 21.976217554863155 27.732726771065362 29.32822139266415 20.962834281407336 50-51 21.687805656526425 27.894621983331714 29.38700935127512 21.030563008866743 52-53 21.95084785928569 28.27173413024784 28.701900683392324 21.07551732707415 54-55 21.445587730573294 28.0095541248189 28.982271320609243 21.56258682399856 56-57 21.985735147508265 28.172651053328323 28.69501306986586 21.146600729297553 58-59 22.18565266170534 27.749983416315843 28.754762401471286 21.309601520507528 60-61 21.805573848347258 27.757866674870385 29.536118302715202 20.900441174067154 62-63 21.60171470487291 27.75796281216983 29.50756552477987 21.13275695817739 64-65 21.510961094196286 28.034405616725582 29.154501292565993 21.30013199651214 66-67 21.530669240582633 28.088675122262615 28.743562406085875 21.63709323106888 68-69 21.502452943195355 27.948747282919573 28.9912601581074 21.557539615777667 70-71 21.816052813986822 28.145588403534394 28.655837120341786 21.382521662137 72-73 21.817831354026566 27.737389430088477 28.926992373428035 21.517786842456925 74-75 21.53706237099576 28.493653496177103 28.33036429306879 21.63891983975835 76-77 21.675644288146557 27.9585052188133 28.655068021946224 21.710782471093918 78-79 21.658531848845247 28.134724888697043 28.56037278199233 21.646370480465382 80-81 21.577103556214844 28.404101601743548 28.483462942435867 21.53533189960574 82-83 21.675974377437687 28.109302551392695 28.514425910205667 21.700297160963952 84-85 21.378669207259918 28.119781537180693 28.45366702013215 22.04788223542724 86-87 21.423187739802476 28.173275945774716 28.44087411878148 21.96266219564133 88-89 21.390020371493755 28.043682866122083 28.276142856181487 22.290153906202683 90-91 21.46801013998974 27.912759166590146 28.49735178581988 22.121878907600234 92-93 21.660855808846893 28.152560615026385 28.478763409718113 21.707820166408613 94-95 21.230384385764374 28.105018463168356 28.408812329416378 22.255784821650888 96-97 21.11708657836776 28.160105135750673 28.46005351002087 22.262754775860692 98-99 21.30045737606022 28.334098430789123 27.832402798574275 22.53304139457638 100-101 21.17149211495692 27.946109978105916 28.201060185171826 22.681337721765335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 517.0 1 356.5 2 256.0 3 348.0 4 324.5 5 308.0 6 553.0 7 1202.5 8 1977.5 9 2386.5 10 2258.0 11 2025.5 12 2087.5 13 2276.0 14 2447.0 15 2588.5 16 2675.0 17 2700.0 18 2828.5 19 3049.0 20 3297.5 21 3645.0 22 4127.5 23 4726.5 24 5473.0 25 6504.5 26 7877.0 27 9483.0 28 10801.0 29 12366.0 30 14266.0 31 15744.0 32 17628.0 33 19699.5 34 21891.5 35 23961.0 36 26517.0 37 28842.5 38 30434.5 39 31272.0 40 31262.0 41 32308.5 42 33627.5 43 34802.0 44 35981.5 45 36720.5 46 36642.0 47 39292.5 48 48059.5 49 47095.0 50 38138.5 51 34338.5 52 31982.0 53 30633.0 54 28982.5 55 26625.5 56 23625.5 57 20589.0 58 18545.0 59 16612.0 60 14333.5 61 11739.5 62 9149.5 63 6982.5 64 5080.5 65 3828.0 66 2879.0 67 2232.0 68 1658.5 69 1079.5 70 801.0 71 659.5 72 553.0 73 482.0 74 394.0 75 301.5 76 220.5 77 146.5 78 109.5 79 83.5 80 52.5 81 32.0 82 20.0 83 12.0 84 10.5 85 8.0 86 2.0 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039320155473240664 2 0.0 3 0.0 4 0.0 5 0.009709867244003197 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006681542311467546 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.884118983367286E-4 22-23 0.0019227459889115239 24-25 0.0 26-27 0.0013459221922380667 28-29 0.0019227459889115239 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.442059491683643E-4 38-39 0.0 40-41 2.4034324861394048E-4 42-43 0.024947629206127024 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.806864972278809E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9227459889115236E-4 84-85 1.9227459889115236E-4 86-87 0.0 88-89 0.0 90-91 0.013315015973212302 92-93 0.0027399130341989215 94-95 0.0 96-97 0.0 98-99 6.248924463962453E-4 100-101 0.016535615504639105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1040179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.28370386434132 #Duplication Level Percentage of deduplicated Percentage of total 1 78.58007303529067 45.013576333919026 2 12.540904839361497 14.367789580177378 3 3.867311187384096 6.646017264280942 4 1.713311315739971 3.9257927215309394 5 0.88747750634246 2.5418999329792786 6 0.5114810400919676 1.7579717059712137 7 0.34812440954385415 1.395929890898117 8 0.23897576348634939 1.0951533493045527 9 0.17028898274583099 0.8779305293074899 >10 1.0181539243829327 10.871875717549194 >50 0.08193365561061551 3.2052689874011575 >100 0.03741399063302602 4.048449839288662 >500 0.0025279718815777183 0.9825949266046775 >1k 0.0016853145877184792 1.873282762412769 >5k 3.370629175436958E-4 1.3964664583745985 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7688 0.7391035581375898 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6777 0.6515224783426699 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4659 0.44790367811693954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4641 0.4461732067269191 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1748 0.16804799943086718 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1460 0.14036045719054124 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1333 0.1281510201609531 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1222 0.1174797799224941 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1197 0.11507634743635471 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1071 0.1029630477062121 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1071 0.1029630477062121 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.442059491683643E-4 0.0 20-21 0.0 0.0 0.0 1.922745988911524E-4 0.0 22-23 0.0 0.0 0.0 4.3261784750509285E-4 0.0 24-25 0.0 0.0 0.0 4.8068649722788096E-4 0.0 26-27 0.0 0.0 0.0 5.287551469506691E-4 0.0 28-29 0.0 0.0 0.0 0.0012497848927924904 0.0 30-31 0.0 0.0 0.0 0.0044703844242192925 0.0 32-33 0.0 0.0 0.0 0.008508151000933493 0.0 34-35 0.0 0.0 0.0 0.012449780278202118 0.0 36-37 0.0 0.0 0.0 0.018506430143273418 0.0 38-39 0.0 0.0 0.0 0.028264366036999403 0.0 40-41 0.0 0.0 0.0 0.041579382010211705 0.0 42-43 0.0 0.0 0.0 0.060566498650713 0.0 44-45 0.0 0.0 0.0 0.07883258554537248 0.0 46-47 0.0 0.0 0.0 0.11272098359993808 0.0 48-49 0.0 0.0 0.0 0.15540594455377393 0.0 50-51 0.0 0.0 0.0 0.18482395818412023 0.0 52-53 0.0 0.0 0.0 0.22630720289488637 0.0 54-55 0.0 0.0 0.0 0.27971147273690394 0.0 56-57 0.0 0.0 0.0 0.3617165891639804 0.0 58-59 0.0 0.0 0.0 0.4543448771797931 0.0 60-61 0.0 0.0 0.0 0.5561062086429355 0.0 62-63 0.0 0.0 0.0 0.6678177505986951 0.0 64-65 0.0 0.0 0.0 0.79169066093432 0.0 66-67 0.0 0.0 0.0 0.937771287441873 0.0 68-69 0.0 0.0 0.0 1.1125969664836532 0.0 70-71 0.0 0.0 0.0 1.2884320871696122 0.0 72-73 0.0 0.0 0.0 1.4734963886023462 0.0 74-75 0.0 0.0 0.0 1.6725967357541345 0.0 76-77 0.0 0.0 0.0 1.9049605885140923 0.0 78-79 0.0 0.0 0.0 2.166838592203842 0.0 80-81 0.0 0.0 0.0 2.495916568206049 0.0 82-83 0.0 0.0 0.0 2.8221104252248894 0.0 84-85 0.0 0.0 0.0 3.200987522339905 0.0 86-87 0.0 0.0 0.0 3.588757319653636 0.0 88-89 0.0 0.0 0.0 4.079009478176353 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 85 1.6324444E-5 33.52824 2 CGTATAG 65 0.0058448166 29.229748 3 GTACATG 6395 0.0 23.92549 1 TACATGG 6500 0.0 23.237652 2 GAGTACT 6365 0.0 22.876734 12-13 AGTACTT 6495 0.0 22.382275 12-13 ACATGGG 6445 0.0 22.18309 3 GTACTTT 6685 0.0 21.81195 14-15 ATGGGCG 110 0.0028777677 21.590155 5 ATGGGAT 1640 0.0 19.984058 5 AGTACAT 5075 0.0 19.748081 2 CATGGGA 4215 0.0 19.720545 4 GTATCAA 13370 0.0 19.617928 1 TACTTTT 7440 0.0 19.375072 14-15 CTATTCC 320 3.965397E-10 19.296202 4 GGTATCA 10540 0.0 19.114843 1 GCACCGT 200 1.2854618E-5 18.998423 6 ACTTTTT 7790 0.0 18.992325 16-17 AGAGTAC 11060 0.0 18.98581 10-11 CATGGGG 2205 0.0 18.310019 4 >>END_MODULE