FastQCFastQC Report
Thu 26 May 2016
SRR937528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759589
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.7389522491768574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.5761010230532564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.37809920891429444No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20000.263300284759258No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17980.23670695599857292No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12740.16772228139164733No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.1575852204284159No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11680.15376736629940665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11040.1453417571871104No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC459.5857703E-442.255483
TGGACCG953.4690187E-530.0256145
TACACCG700.00835493827.1660335
GGACCGT908.924311E-426.3992166
GTACATG44850.025.8723051
GTATCAA74750.025.5754091
TACATGG45600.025.1239432
ACATGGG45450.024.7884883
GGTATCA55100.024.252831
CATGGGG15700.023.6204384
GTATAGG1652.4032124E-623.0575831
GAGTACT42850.022.72450812-13
AGTACTT43300.022.70774312-13
CATGGGA26600.021.9844914
TATCAAC88550.021.4200212
TCAACGC88350.021.4175344
ATCAACG88400.021.4025943
CAACGCA89050.021.2477725
GTACTTT45500.021.14000714-15
AACGCAG90950.020.9510216
Produced by FastQC (version 0.11.5)