##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937528_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759589 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91327941821169 33.0 31.0 34.0 30.0 34.0 2 32.012999135058564 33.0 31.0 34.0 30.0 34.0 3 32.04630398807776 34.0 31.0 34.0 30.0 34.0 4 35.64310173001452 37.0 35.0 37.0 35.0 37.0 5 35.64287660827105 37.0 35.0 37.0 35.0 37.0 6 35.71604249139995 37.0 35.0 37.0 35.0 37.0 7 35.64882324520234 37.0 35.0 37.0 35.0 37.0 8 35.65268717688118 37.0 35.0 37.0 33.0 37.0 9 37.220454745921806 39.0 37.0 39.0 34.0 39.0 10-11 37.17034146097429 39.0 37.0 39.0 34.0 39.0 12-13 37.08054092410501 39.0 37.0 39.0 34.0 39.0 14-15 38.22176334833706 40.0 38.0 41.0 33.5 41.0 16-17 38.158846428792415 40.0 38.0 41.0 33.0 41.0 18-19 38.13199374925124 40.0 38.0 41.0 33.5 41.0 20-21 38.164780558960175 40.0 38.0 41.0 34.0 41.0 22-23 38.15541957558627 40.0 38.0 41.0 34.0 41.0 24-25 38.08010647863516 40.0 38.0 41.0 34.0 41.0 26-27 37.97287941241908 40.0 38.0 41.0 33.5 41.0 28-29 37.817895598804085 40.0 38.0 41.0 33.0 41.0 30-31 37.5564469732974 40.0 37.0 41.0 33.0 41.0 32-33 37.39755709995801 40.0 37.0 41.0 33.0 41.0 34-35 37.371797116598586 40.0 37.0 41.0 33.0 41.0 36-37 37.205261661240485 40.0 36.5 41.0 32.0 41.0 38-39 37.02167487944138 40.0 36.0 41.0 31.5 41.0 40-41 36.79060649904093 39.5 36.0 41.0 31.0 41.0 42-43 36.496825915067234 39.0 36.0 41.0 30.0 41.0 44-45 36.178218088992864 39.0 35.0 41.0 30.0 41.0 46-47 35.712330615635565 39.0 35.0 41.0 28.0 41.0 48-49 35.286905155287926 39.0 35.0 40.5 26.0 41.0 50-51 34.36110580853593 38.0 34.0 39.5 23.5 40.5 52-53 34.45321022289686 38.0 34.5 40.0 24.0 40.5 54-55 34.71041181481038 39.0 35.0 40.0 24.0 41.0 56-57 34.47340667123932 38.0 34.0 40.0 23.0 41.0 58-59 34.210403915801834 38.0 34.0 40.0 22.5 41.0 60-61 33.85177181344122 38.0 34.0 40.0 20.5 41.0 62-63 33.50900750274161 37.5 33.5 40.0 19.5 41.0 64-65 33.074825991424305 37.0 33.0 40.0 17.5 41.0 66-67 32.54352419532142 36.0 32.0 39.0 11.5 41.0 68-69 32.11466727401266 36.0 31.5 39.0 8.0 40.0 70-71 31.6714539046774 35.0 31.0 38.5 7.0 40.0 72-73 31.20013783769907 35.0 31.0 38.0 3.5 40.0 74-75 30.66451528392328 35.0 30.5 37.0 2.0 39.0 76-77 30.158508087926496 34.5 30.0 36.5 2.0 39.0 78-79 29.65520498585419 34.0 29.0 36.0 2.0 38.5 80-81 29.135964317545408 34.0 29.0 35.5 2.0 37.5 82-83 28.626395326946543 34.0 28.5 35.0 2.0 37.0 84-85 28.10310049250318 34.0 27.0 35.0 2.0 36.5 86-87 27.55688010226583 33.5 26.0 35.0 2.0 36.0 88-89 27.029394843790524 33.0 25.5 35.0 2.0 36.0 90-91 26.469531549298374 32.5 25.0 35.0 2.0 35.0 92-93 25.825876230435142 32.0 23.5 34.5 2.0 35.0 94-95 25.092912087984423 32.0 19.0 34.0 2.0 35.0 96-97 24.260573152059862 31.0 9.0 34.0 2.0 35.0 98-99 23.135084894594314 31.0 2.0 34.0 2.0 35.0 100-101 20.017802390503284 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19423616055524562 1101 2 0.010915580750651799 1101 3 0.05479952585636383 1101 4 0.06973332657585019 1101 5 -0.010319123904679373 1101 6 0.02129108269463842 1101 7 0.05884276080600159 1101 8 0.15663444792119918 1101 9 0.2628593438540605 1101 10-11 0.2600245815793656 1101 12-13 0.16586597050238794 1101 14-15 0.23086804887526569 1101 16-17 0.3350457120761732 1101 18-19 0.36330030795533474 1101 20-21 0.2780074791680889 1101 22-23 0.28785833702956864 1101 24-25 0.0553507418277448 1101 26-27 0.1995857402685388 1101 28-29 0.19141675212540576 1101 30-31 0.2876521241154748 1101 32-33 0.28728737850263286 1101 34-35 0.36108888264731576 1101 36-37 0.48872986954649633 1101 38-39 0.5382978713273943 1101 40-41 0.528340200448234 1101 42-43 0.323507181107324 1101 44-45 0.5001269354014539 1101 46-47 0.5490847449258283 1101 48-49 0.6771036680142046 1101 50-51 0.48013291032110317 1101 52-53 0.5841270370131255 1101 54-55 0.6192286204131392 1101 56-57 0.7372483076837995 1101 58-59 0.6972194236202895 1101 60-61 0.6126805613695083 1101 62-63 0.6012025555275002 1101 64-65 0.6933661975680465 1101 66-67 0.8249308781491251 1101 68-69 0.7144298088298591 1101 70-71 0.7383069951567443 1101 72-73 0.8239894518676785 1101 74-75 0.8858662181529553 1101 76-77 0.8168733999858837 1101 78-79 1.0466718754588555 1101 80-81 1.1432851261899266 1101 82-83 1.220236507087101 1101 84-85 1.2895129745999547 1101 86-87 1.5497824119578212 1101 88-89 1.6100793079255702 1101 90-91 1.6046794269494846 1101 92-93 1.618062606275032 1101 94-95 1.8107011958216148 1101 96-97 1.8177339074956826 1101 98-99 2.164033547238308 1101 100-101 2.738766918126469 1102 1 0.07685600622983557 1102 2 0.05317985448597895 1102 3 -0.013866408094546756 1102 4 0.11097475641831522 1102 5 0.16843507726448337 1102 6 0.08250129247434757 1102 7 0.09588814139723212 1102 8 0.05500085586183445 1102 9 0.24233078728135382 1102 10-11 0.19846451140249144 1102 12-13 0.01840464856483237 1102 14-15 0.22309432151379127 1102 16-17 0.30492984286366465 1102 18-19 0.1145842671690076 1102 20-21 0.20683791008232078 1102 22-23 0.10705503480252077 1102 24-25 0.08313989717941439 1102 26-27 0.22086009680984375 1102 28-29 0.1372424340403029 1102 30-31 0.312655480499771 1102 32-33 0.38910414950165517 1102 34-35 0.4730635516168107 1102 36-37 0.3860017548787553 1102 38-39 0.5964492203663312 1102 40-41 0.5058916782813085 1102 42-43 0.628728235990458 1102 44-45 0.7375001038964939 1102 46-47 0.7817720596135942 1102 48-49 0.8948744144513228 1102 50-51 0.9058214242051577 1102 52-53 0.9727269355251593 1102 54-55 0.995843382587708 1102 56-57 0.987408162596978 1102 58-59 0.9367372647939121 1102 60-61 1.0379310386587335 1102 62-63 0.918963403475324 1102 64-65 0.9654005760453188 1102 66-67 1.0502620224372379 1102 68-69 1.2023883535016218 1102 70-71 1.080167820121872 1102 72-73 1.0084267511957172 1102 74-75 0.962062968262746 1102 76-77 1.116372104080213 1102 78-79 1.3683170271332905 1102 80-81 1.4270310103525716 1102 82-83 1.411695476142377 1102 84-85 1.4858131013461282 1102 86-87 1.4467220721324452 1102 88-89 1.580621631362689 1102 90-91 1.7195610660482892 1102 92-93 1.6627628185805605 1102 94-95 1.9097128259776497 1102 96-97 2.055661163839247 1102 98-99 1.9662867619718085 1102 100-101 2.5228370631450296 1103 1 0.039701856032205995 1103 2 -0.13232739189083276 1103 3 0.05471066700426874 1103 4 -0.049763056493411284 1103 5 0.019554971862639547 1103 6 0.012606694318876066 1103 7 0.04878669212186537 1103 8 -0.018780435310759458 1103 9 0.09582222338148227 1103 10-11 0.03239073011131666 1103 12-13 -0.001259383055717933 1103 14-15 0.01215888014357347 1103 16-17 0.3128679192800021 1103 18-19 0.12924171117427363 1103 20-21 0.2284123501350237 1103 22-23 2.697910607594167E-4 1103 24-25 0.02464187346399882 1103 26-27 0.08999711920773734 1103 28-29 -0.08854807979369639 1103 30-31 0.006891317126914487 1103 32-33 0.232154215377804 1103 34-35 0.06473021828347214 1103 36-37 0.1537883161435758 1103 38-39 0.15135699375237266 1103 40-41 0.2562869351982968 1103 42-43 0.24117882887583875 1103 44-45 0.44599812761190805 1103 46-47 0.554565208493699 1103 48-49 0.6032406858610742 1103 50-51 0.21807438863203998 1103 52-53 0.4153158683314828 1103 54-55 0.42351136677743284 1103 56-57 0.7335544076562641 1103 58-59 0.7252418761246062 1103 60-61 0.6809811045348866 1103 62-63 0.8231794772566161 1103 64-65 0.7550580200505834 1103 66-67 0.9504596508957306 1103 68-69 1.1421775498125548 1103 70-71 1.1641599149835287 1103 72-73 0.9245664086397234 1103 74-75 0.936832401727834 1103 76-77 1.011200826082849 1103 78-79 0.9880469349066807 1103 80-81 1.4559506414461154 1103 82-83 1.3473015367484393 1103 84-85 1.4910173174199066 1103 86-87 1.436346578061297 1103 88-89 1.5760432387408159 1103 90-91 1.6573739777742453 1103 92-93 1.5338431874870153 1103 94-95 1.5704506388893762 1103 96-97 1.5132039833385917 1103 98-99 1.6569323482695673 1103 100-101 2.09217171400142 1104 1 0.17013183971586088 1104 2 0.03641960878101713 1104 3 0.07488534787575318 1104 4 -0.052882357769924226 1104 5 0.15940764486389014 1104 6 0.1997215805843524 1104 7 0.3273882853649823 1104 8 0.15646430986632254 1104 9 0.3947544625599093 1104 10-11 0.18713686697666532 1104 12-13 0.09500585353128344 1104 14-15 0.17776138518243556 1104 16-17 0.1563125876742859 1104 18-19 0.22452436785917484 1104 20-21 0.300864120938364 1104 22-23 0.12222727258489385 1104 24-25 0.2339613858932168 1104 26-27 0.20522650782468332 1104 28-29 0.07439941996636179 1104 30-31 0.11466437596173051 1104 32-33 0.1725995556215878 1104 34-35 0.2997743683938481 1104 36-37 0.3198011292734364 1104 38-39 0.5350142545259544 1104 40-41 0.6489661750485709 1104 42-43 0.5779098011380484 1104 44-45 0.6565569624329086 1104 46-47 0.5903243984351505 1104 48-49 0.6674239088191243 1104 50-51 0.6238654914250148 1104 52-53 0.6121149853292778 1104 54-55 0.7297151771239143 1104 56-57 0.7503794388492793 1104 58-59 0.732664613431453 1104 60-61 1.0357164438188846 1104 62-63 1.219337937761459 1104 64-65 1.0710276428550785 1104 66-67 1.2703739900837547 1104 68-69 1.2495606959878138 1104 70-71 1.2156583793275217 1104 72-73 1.3159691344291424 1104 74-75 1.1734398016702485 1104 76-77 1.3375349272441888 1104 78-79 1.2396665721079465 1104 80-81 1.3445543942054954 1104 82-83 1.4173178530935786 1104 84-85 1.8558244006332671 1104 86-87 1.7905792299472765 1104 88-89 2.023390632924521 1104 90-91 2.013695361784226 1104 92-93 1.8723689577144853 1104 94-95 1.7919450241470756 1104 96-97 2.086943065784123 1104 98-99 2.092667543585815 1104 100-101 2.5485134934428224 1105 1 0.20465894059671896 1105 2 0.19756215362766838 1105 3 0.0977105262979201 1105 4 0.06449049634182558 1105 5 -0.0745423969286918 1105 6 0.04697773695290408 1105 7 -0.0038902461080496664 1105 8 -0.10077705835846018 1105 9 0.045481010492785856 1105 10-11 0.050756425904197044 1105 12-13 0.08162017231222052 1105 14-15 0.12722852978476595 1105 16-17 0.10552656568495422 1105 18-19 0.009324727918325948 1105 20-21 -0.035755020063369614 1105 22-23 -0.10227523496991608 1105 24-25 -0.05856658298738893 1105 26-27 -0.007911632726980145 1105 28-29 -0.06940322261063159 1105 30-31 0.21063058826803882 1105 32-33 0.13207682688758382 1105 34-35 0.3543337781540217 1105 36-37 0.30445843011571583 1105 38-39 0.4216679547767157 1105 40-41 0.47357798627470515 1105 42-43 0.3372126687520165 1105 44-45 0.7374537347597894 1105 46-47 0.8152729614133563 1105 48-49 1.1529170612656046 1105 50-51 1.45828650344842 1105 52-53 1.178099039852519 1105 54-55 1.3193200765002402 1105 56-57 1.31183462434403 1105 58-59 1.5748373453802742 1105 60-61 1.5492307175924296 1105 62-63 1.6817045229861805 1105 64-65 1.580627880751301 1105 66-67 1.6347409932342742 1105 68-69 1.5568004568056644 1105 70-71 1.576561420541431 1105 72-73 1.527459682876394 1105 74-75 1.530553381044001 1105 76-77 1.4908212707149033 1105 78-79 1.8889544908374525 1105 80-81 1.717034783840937 1105 82-83 1.8616975547588481 1105 84-85 1.7010954094418587 1105 86-87 1.9341568355539138 1105 88-89 2.0512068098678746 1105 90-91 2.1153959983173714 1105 92-93 2.478244833751262 1105 94-95 2.6851087224689465 1105 96-97 2.616463094074483 1105 98-99 2.5612467565993846 1105 100-101 3.0799302619119295 1106 1 -0.18998697602235382 1106 2 -0.26163259223183744 1106 3 -0.1694368180767185 1106 4 -0.053641999374910654 1106 5 -0.20373999829934064 1106 6 0.02628955876900818 1106 7 0.059059496895947916 1106 8 -0.11865255807290964 1106 9 -0.16918203919311026 1106 10-11 -0.48697201498671916 1106 12-13 -0.5854772006175253 1106 14-15 -0.6407183577080957 1106 16-17 -0.6130485342579348 1106 18-19 -0.11271267927755702 1106 20-21 -0.22002115370129616 1106 22-23 -0.17398425838511145 1106 24-25 -0.6241219799034283 1106 26-27 -0.5488882175356551 1106 28-29 -0.07650630656266344 1106 30-31 -0.06560442029849156 1106 32-33 -0.10263904975433746 1106 34-35 -0.21272076210868107 1106 36-37 -0.5114610444702308 1106 38-39 -0.42167156549741236 1106 40-41 -0.08140488065847507 1106 42-43 0.034678402618375515 1106 44-45 -0.027680474263625854 1106 46-47 -0.0012318929555803493 1106 48-49 0.4302014701748007 1106 50-51 0.0630552070463608 1106 52-53 0.21139429970403256 1106 54-55 0.2935348109377003 1106 56-57 0.3291532993868671 1106 58-59 0.14975168004617956 1106 60-61 0.29656081467981465 1106 62-63 0.27363770402558174 1106 64-65 0.47149024511452353 1106 66-67 0.7399737173918979 1106 68-69 0.48206459158579307 1106 70-71 0.6197438877030557 1106 72-73 0.5586989747096283 1106 74-75 0.5133988978915163 1106 76-77 0.6115951142328946 1106 78-79 0.9165315896381365 1106 80-81 0.7674702492892536 1106 82-83 0.6067218266035113 1106 84-85 0.3987538903099299 1106 86-87 0.3609629463477475 1106 88-89 0.9526417042139634 1106 90-91 1.38645539294901 1106 92-93 1.1312217041110557 1106 94-95 0.9898560097333657 1106 96-97 0.9396212893914466 1106 98-99 1.1777230558569514 1106 100-101 1.8006755503185552 1107 1 0.038734129033084486 1107 2 0.15467543257552663 1107 3 0.12341696880544717 1107 4 0.06735969203647585 1107 5 0.028213581854764413 1107 6 0.036644755160665454 1107 7 0.16541168633402492 1107 8 0.13610218727571066 1107 9 -0.1567313720252841 1107 10-11 -0.1600480345319184 1107 12-13 0.062373297409159534 1107 14-15 0.26276313073393 1107 16-17 0.2640845234379867 1107 18-19 0.19675687531744757 1107 20-21 0.044654189279349055 1107 22-23 0.07925761344349525 1107 24-25 0.2110311867945498 1107 26-27 0.17469522542619842 1107 28-29 0.18885734688593914 1107 30-31 0.2544129636518946 1107 32-33 0.1687831453217754 1107 34-35 0.4405821884645178 1107 36-37 0.5578374896399012 1107 38-39 0.5668996617155173 1107 40-41 0.3774773783153975 1107 42-43 0.5971055779613579 1107 44-45 0.9061430551481422 1107 46-47 0.8561163498559239 1107 48-49 0.2306102279270945 1107 50-51 0.4376534968415129 1107 52-53 0.49899531455086077 1107 54-55 0.25984048656258096 1107 56-57 0.6579895143840488 1107 58-59 0.44491706249308294 1107 60-61 0.8377748721878007 1107 62-63 0.9132660633565379 1107 64-65 0.7215325303992373 1107 66-67 1.0200526499233789 1107 68-69 1.07346893287502 1107 70-71 1.2705520064777218 1107 72-73 1.2636158136496753 1107 74-75 1.108265934162894 1107 76-77 1.0681751743719623 1107 78-79 1.1059626176403903 1107 80-81 1.5222978192948915 1107 82-83 1.6325205486482375 1107 84-85 1.6356971340611075 1107 86-87 1.5919063780335172 1107 88-89 1.712794870911953 1107 90-91 1.576962346529168 1107 92-93 1.6692925554547884 1107 94-95 1.9892240796450338 1107 96-97 2.042625893789115 1107 98-99 2.368403848940961 1107 100-101 2.9817223460336457 1108 1 -0.0026859711674376285 1108 2 -0.27738566889436456 1108 3 -0.12024691178790903 1108 4 -0.25969935513871434 1108 5 -0.2508976137447476 1108 6 -0.059382790785015516 1108 7 -0.25811336073078905 1108 8 -0.19177551166893636 1108 9 0.04643837403936146 1108 10-11 -0.15564509842355534 1108 12-13 -0.017777923209827406 1108 14-15 -0.0980430726228505 1108 16-17 -0.0027882881249396974 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587.0 5 574.0 6 683.0 7 786.0 8 918.0 9 1186.0 10 1413.0 11 1883.0 12 2793.0 13 3763.0 14 4251.0 15 5648.0 16 7570.0 17 10759.0 18 9305.0 19 5636.0 20 5187.0 21 5132.0 22 5783.0 23 6262.0 24 7025.0 25 7852.0 26 8981.0 27 10682.0 28 12406.0 29 14987.0 30 18437.0 31 23392.0 32 29745.0 33 39451.0 34 53645.0 35 76977.0 36 110441.0 37 146847.0 38 104286.0 39 10366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.326590141367916 19.00547055924942 15.076866575688841 21.591072723693824 2 14.87323095691714 20.202244253704443 41.56596043575332 23.358564353625095 3 19.385743578774473 25.500448261156027 30.409799160517103 24.7040089995524 4 12.01656163810233 16.608112086800727 38.40625920072668 32.96906707437026 5 12.865200583053426 38.027864038701225 34.376254304244036 14.730681074001309 6 30.291267259976646 36.57881368791192 17.894563231054434 15.235355821057006 7 27.31361458865189 34.014240875825735 20.90495660055491 17.76718793496746 8 26.055422809033686 34.19824545894746 19.964930289384487 19.781401442634373 9 26.41725270810518 17.045031731999842 21.263606496813605 35.27410906308137 10-11 24.725122479327283 26.883898965935376 27.02217888513135 21.368799669605995 12-13 26.25124197253608 24.595872362828832 27.890447796249212 21.262437868385877 14-15 22.831272245074334 25.517405172897213 27.52296664764462 24.128355934383833 16-17 21.070099089845815 29.183513127870825 27.902621352242267 21.843766430041093 18-19 20.740810123376008 27.143117223037223 31.45943050732974 20.65664214625703 20-21 21.934531033973258 25.80797300292345 31.3928085191713 20.864687443931988 22-23 21.320669583251973 25.888300763624656 31.434012697172953 21.357016955950414 24-25 21.36695360083804 26.08042292720351 31.58107567000965 20.971547801948795 26-27 21.06286031693047 26.14389390000218 31.767064484484976 21.026181298582383 28-29 20.55955026973392 26.593580880262152 31.887913136243174 20.95895571376075 30-31 20.72253768948653 26.308848279488384 31.674210831533884 21.294403199491203 32-33 20.59604719927483 26.42431055571169 31.55082577619796 21.42881646881552 34-35 21.373526580756934 26.2892892556095 31.224975705529246 21.112208458104327 36-37 21.22540010897371 26.074927999514504 31.483242983663178 21.216428907848613 38-39 20.99591783968039 26.48688187986484 31.473330743370038 21.043869537084735 40-41 20.99870396691653 26.615243063914544 31.475927751926736 20.910125217242186 42-43 20.790806314260514 26.652955117985062 30.83746207941045 21.718776488343973 44-45 20.914135920511296 26.78541862770772 30.63762336006396 21.662822091717025 46-47 21.283984138701264 27.00016030176312 30.19854329895751 21.5173122605781 48-49 21.405744264510094 27.175079070393434 29.79017481913101 21.629001845965465 50-51 21.18688234750828 27.459658798538083 29.852555052710745 21.50090380124289 52-53 21.461033262823413 27.659858038856548 29.310529114702437 21.568579583617595 54-55 20.958453976252063 27.399318000550192 29.602655166749457 22.039572856448288 56-57 21.326980772820118 27.669846244556435 29.22184431346245 21.781328669160995 58-59 21.621956679857327 27.244264172816337 29.418184383937245 21.715594763389092 60-61 21.48817398897532 27.154383822022194 30.02953369994341 21.327908489059077 62-63 21.343780459977815 27.183325045801254 30.00658166340661 21.466312830814317 64-65 21.04135724140842 27.41496796047095 29.803159369286895 21.740515428833728 66-67 21.155871658924934 27.470435108930573 29.31143531931929 22.062257912825203 68-69 20.92093808531406 27.336046112855144 29.70104466193579 22.041971139895004 70-71 21.21410382314358 27.33224157819515 29.411632798294168 22.0420218003671 72-73 21.22533328144448 27.127734306205358 29.692208954652884 21.954723457697284 74-75 20.910633846064542 27.682208233646595 29.260045686045967 22.1471122342429 76-77 21.07012579238649 27.330985032882804 29.285549376974785 22.313339797755923 78-79 21.142782513023942 27.316968920632114 29.376460205243347 22.163788361100593 80-81 21.13119560449708 27.513909088810394 29.237283943288517 22.11761136340401 82-83 21.13044734205783 27.34656890903779 29.354988270535465 22.167995478368915 84-85 21.013371635987614 27.22739694950486 29.246068174503936 22.513163240003585 86-87 21.06839075266959 27.27775165326402 29.307241649260707 22.34661594480568 88-89 21.03908935727873 27.151484451057833 28.990987496998912 22.818438694664525 90-91 20.94579041546105 27.088303364198364 29.316020422498518 22.649885797842064 92-93 21.263611643672544 27.307837655489802 29.2217612185797 22.206789482257953 94-95 20.95931049310981 27.185674317741427 29.081185109753637 22.773830079395125 96-97 20.76505759863333 27.247896734703303 29.179042210415574 22.808003456247793 98-99 21.039637755960175 27.47764831591479 28.461089436417662 23.021624491707367 100-101 21.338227545241896 26.6080565885558 29.12080266482188 22.93291320138042 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1063.0 1 650.5 2 272.0 3 295.5 4 231.0 5 221.0 6 380.0 7 874.0 8 1544.5 9 1862.5 10 1665.5 11 1425.0 12 1445.5 13 1551.0 14 1704.0 15 1786.5 16 1843.5 17 1864.0 18 1879.0 19 2059.0 20 2263.5 21 2476.5 22 2829.0 23 3324.0 24 3949.0 25 4757.0 26 5782.5 27 6941.5 28 7927.5 29 9050.5 30 10487.0 31 11749.0 32 13005.0 33 14466.0 34 16139.0 35 17552.5 36 19291.5 37 21250.0 38 22260.5 39 22502.5 40 22678.0 41 23650.5 42 24451.5 43 25063.5 44 25984.5 45 26830.0 46 27201.5 47 28952.0 48 34550.5 49 34300.0 50 28245.5 51 25148.5 52 23538.5 53 22710.5 54 21580.0 55 19634.5 56 17334.5 57 15051.5 58 13389.0 59 11839.5 60 10159.0 61 8485.0 62 6751.0 63 5000.0 64 3636.0 65 2826.0 66 2128.5 67 1615.0 68 1180.0 69 807.0 70 602.5 71 484.0 72 412.5 73 333.0 74 241.5 75 204.0 76 151.0 77 101.0 78 77.0 79 49.0 80 29.5 81 20.0 82 16.5 83 12.5 84 12.0 85 10.5 86 6.0 87 3.0 88 3.0 89 3.0 90 1.5 91 0.0 92 0.5 93 2.0 94 1.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34597657417366495 2 0.2880505115266282 3 0.2920000157980171 4 0.28647070981807266 5 0.28923536280804485 6 0.2230153411910915 7 0.21458973207879525 8 0.2198557377739804 9 0.22104058905539709 10-11 0.22459514289964708 12-13 0.2267015451777211 14-15 0.22334446654704054 16-17 0.21597205857378138 18-19 0.20813887510219342 20-21 0.20813887510219342 22-23 0.2139314813668971 24-25 0.21505050757712396 26-27 0.2188683617061332 28-29 0.22545086882511464 30-31 0.22624076967939238 32-33 0.22327864147585075 34-35 0.2238710671165591 36-37 0.2116934289464434 38-39 0.20201714348154065 40-41 0.19833093949491107 42-43 0.2093895514547999 44-45 0.2099161520243184 46-47 0.21636700900092024 48-49 0.22643824489296185 50-51 0.22104058905539709 52-53 0.23381065286622107 54-55 0.21992156284517023 56-57 0.19345988422686478 58-59 0.17779351728368895 60-61 0.19378900958281386 62-63 0.1872065024638324 64-65 0.1926041583013972 66-67 0.19332823408448516 68-69 0.1907610563080824 70-71 0.19517133607779996 72-73 0.20517674689865176 74-75 0.20925790131242028 76-77 0.2098503269531286 78-79 0.20563752239698047 80-81 0.20293869447819807 82-83 0.19161678223355 84-85 0.18654825175193426 86-87 0.19319658394210554 88-89 0.20155636798321197 90-91 0.19793598906777216 92-93 0.19227503294544812 94-95 0.19510551100661017 96-97 0.20293869447819807 98-99 0.20228044376629992 100-101 0.1862191263959852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.0354981402615 #Duplication Level Percentage of deduplicated Percentage of total 1 82.36712217649362 51.92052576775056 2 10.783424321276017 13.594770474988904 3 3.0327074587988276 5.735046761372121 4 1.227455591111696 3.0949309852309965 5 0.654637081710055 2.0632687273340196 6 0.3836273886432663 1.4509286126025593 7 0.27470014624075 1.2121102390241854 8 0.19753936851685042 0.9961593997417884 9 0.1423812042489721 0.8077563122079847 >10 0.8484403119378601 9.984041814767398 >50 0.05787760465240361 2.5061506538182527 >100 0.026535367981554096 3.150810056765166 >500 0.0016715192414602563 0.6647217468001388 >1k 0.0016715192414602563 2.079510929309965 >5k 2.0893990518253204E-4 0.7392675182859479 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5613 0.7389522491768574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4376 0.5761010230532564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2872 0.37809920891429444 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2000 0.263300284759258 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1798 0.23670695599857292 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1274 0.16772228139164733 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1197 0.1575852204284159 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1168 0.15376736629940665 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1104 0.1453417571871104 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.3165014237962898E-4 0.0 24-25 0.0 0.0 0.0 1.9747521356944345E-4 0.0 26-27 0.0 0.0 0.0 4.607754983287014E-4 0.0 28-29 0.0 0.0 0.0 0.0017772769221249913 0.0 30-31 0.0 0.0 0.0 0.004015329342578684 0.0 32-33 0.0 0.0 0.0 0.007438233044449038 0.0 34-35 0.0 0.0 0.0 0.011519387458217536 0.0 36-37 0.0 0.0 0.0 0.018101894577198983 0.0 38-39 0.0 0.0 0.0 0.027909830184481343 0.0 40-41 0.0 0.0 0.0 0.04061406892411554 0.0 42-43 0.0 0.0 0.0 0.05963751449797193 0.0 44-45 0.0 0.0 0.0 0.07984581135324498 0.0 46-47 0.0 0.0 0.0 0.11486474922622628 0.0 48-49 0.0 0.0 0.0 0.15903337199459183 0.0 50-51 0.0 0.0 0.0 0.18885212924357778 0.0 52-53 0.0 0.0 0.0 0.23170425058814703 0.0 54-55 0.0 0.0 0.0 0.28943283802161435 0.0 56-57 0.0 0.0 0.0 0.37888910976857226 0.0 58-59 0.0 0.0 0.0 0.48388009831632633 0.0 60-61 0.0 0.0 0.0 0.601114550105386 0.0 62-63 0.0 0.0 0.0 0.7363192463292649 0.0 64-65 0.0 0.0 0.0 0.8852813824318151 0.0 66-67 0.0 0.0 0.0 1.0547151156743977 0.0 68-69 0.0 0.0 0.0 1.2739784278076698 0.0 70-71 0.0 0.0 0.0 1.4891605855271735 0.0 72-73 0.0 0.0 0.0 1.7250776406714685 0.0 74-75 0.0 0.0 0.0 1.978240864467495 0.0 76-77 0.0 0.0 0.0 2.2796538654456553 0.0 78-79 0.0 0.0 0.0 2.6227341364869687 0.0 80-81 0.0 0.0 0.0 3.0463842946646147 0.0 82-83 0.0 0.0 0.0 3.4695078522727423 0.0 84-85 0.0 0.0 0.0 3.9502941722431473 0.0 86-87 0.0 0.0 0.0 4.423905559453862 0.0 88-89 0.0 0.0 0.0 5.046413257695939 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 45 9.5857703E-4 42.25548 3 TGGACCG 95 3.4690187E-5 30.025614 5 TACACCG 70 0.008354938 27.166033 5 GGACCGT 90 8.924311E-4 26.399216 6 GTACATG 4485 0.0 25.872305 1 GTATCAA 7475 0.0 25.575409 1 TACATGG 4560 0.0 25.123943 2 ACATGGG 4545 0.0 24.788488 3 GGTATCA 5510 0.0 24.25283 1 CATGGGG 1570 0.0 23.620438 4 GTATAGG 165 2.4032124E-6 23.057583 1 GAGTACT 4285 0.0 22.724508 12-13 AGTACTT 4330 0.0 22.707743 12-13 CATGGGA 2660 0.0 21.984491 4 TATCAAC 8855 0.0 21.420021 2 TCAACGC 8835 0.0 21.417534 4 ATCAACG 8840 0.0 21.402594 3 CAACGCA 8905 0.0 21.247772 5 GTACTTT 4550 0.0 21.140007 14-15 AACGCAG 9095 0.0 20.951021 6 >>END_MODULE