FastQCFastQC Report
Thu 26 May 2016
SRR937528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759589
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55920.7361875961868853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50210.6610153648881172No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36660.48262942196371983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.43957982540558116No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.1740414882258695No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10860.1429720546242771No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9780.12875383924727715No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9360.12322453326733274No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8650.11387737315837908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.11005951902936983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT1850.033.3787274
TAAGGTG2600.025.5771885
GTGTAGC3000.025.3369331
GTACATG45600.023.7533741
GTACACG1000.001651764423.7533741
GAGTACT47300.023.4505212-13
ACATGGG45600.022.7085693
GTCTTAG2103.4371624E-822.6222611
TACATGG46800.022.5322842
GTACTTT50200.022.18751514-15
CATGGGG13550.021.7345454
GGTATCA75950.021.4546621
AGTACTT48800.020.73416112-13
GGGATCG1150.003720488620.6537487
CGTGAAC1150.003721908220.652393
AACCTCG1656.3153995E-520.1530517
TAGCACT3353.45608E-1119.8509544
AGGTAAG2651.8519131E-819.7171862
ACTTTTT58300.019.3492716-17
GTATCAA96250.019.2494871
Produced by FastQC (version 0.11.5)