##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937528_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759589 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.125799610052276 33.0 31.0 34.0 31.0 34.0 2 32.37240402375495 34.0 31.0 34.0 31.0 34.0 3 32.44929297291035 34.0 31.0 34.0 31.0 34.0 4 36.08912977939386 37.0 35.0 37.0 35.0 37.0 5 35.929833107114504 37.0 35.0 37.0 35.0 37.0 6 36.04029415907813 37.0 35.0 37.0 35.0 37.0 7 35.99517107277752 37.0 35.0 37.0 35.0 37.0 8 35.997738250553915 37.0 35.0 37.0 35.0 37.0 9 37.65176562588452 39.0 37.0 39.0 35.0 39.0 10-11 37.58556008578323 39.0 37.0 39.0 35.0 39.0 12-13 37.49909358876972 39.0 37.0 39.0 35.0 39.0 14-15 38.6394161842786 40.0 38.0 41.0 34.0 41.0 16-17 38.614339465158125 40.0 38.0 41.0 34.5 41.0 18-19 38.6480300530945 40.0 38.0 41.0 34.0 41.0 20-21 38.5846622318122 40.0 38.0 41.0 34.0 41.0 22-23 38.4291965786761 40.0 38.0 41.0 34.0 41.0 24-25 38.34802373388767 40.0 38.0 41.0 34.0 41.0 26-27 38.25033669523914 40.0 38.0 41.0 34.0 41.0 28-29 38.128103487543925 40.0 38.0 41.0 34.0 41.0 30-31 38.18546937883514 40.0 37.5 41.0 34.0 41.0 32-33 38.06665578358823 40.0 37.0 41.0 34.0 41.0 34-35 37.89877618027644 40.0 37.0 41.0 33.0 41.0 36-37 37.70323424904784 40.0 37.0 41.0 33.0 41.0 38-39 37.545019740938855 40.0 37.0 41.0 33.0 41.0 40-41 37.34494509530812 40.0 36.0 41.0 33.0 41.0 42-43 37.09147973443534 40.0 36.0 41.0 32.0 41.0 44-45 36.712784808626765 39.0 36.0 41.0 31.0 41.0 46-47 36.234201653789086 39.0 35.0 41.0 30.0 41.0 48-49 35.79480284732928 39.0 35.0 40.5 28.5 41.0 50-51 35.488528006593036 39.0 35.0 40.0 27.5 41.0 52-53 35.25717394538363 39.0 35.0 40.0 27.0 41.0 54-55 35.04510663003282 38.5 35.0 40.0 26.5 41.0 56-57 34.74267070744837 38.0 34.0 40.0 26.0 41.0 58-59 34.4724265359293 38.0 34.0 40.0 25.0 41.0 60-61 34.095807732866064 38.0 34.0 40.0 24.0 41.0 62-63 33.734857271498136 37.0 33.0 40.0 23.0 41.0 64-65 33.319357573635216 36.5 33.0 39.0 22.0 41.0 66-67 32.87646543064736 36.0 32.5 39.0 20.5 40.0 68-69 32.39176383544259 35.5 32.0 39.0 19.5 40.0 70-71 31.960495741776143 35.0 31.0 38.0 18.0 40.0 72-73 31.498098313693326 35.0 31.0 37.5 15.5 39.5 74-75 30.998268800627706 35.0 30.5 37.0 11.5 39.0 76-77 29.81361499442462 33.5 29.5 35.5 9.0 38.0 78-79 30.361956268455703 34.0 30.0 36.0 7.5 38.0 80-81 30.14619090060546 34.0 30.0 35.5 7.0 37.0 82-83 29.79367987161478 34.0 30.0 35.0 2.0 37.0 84-85 29.376433834613195 34.0 29.0 35.0 2.0 36.5 86-87 28.927412719246853 34.0 29.0 35.0 2.0 36.0 88-89 28.45202273861259 34.0 29.0 35.0 2.0 35.5 90-91 27.902068750337357 33.0 27.0 35.0 2.0 35.0 92-93 27.41678131199899 33.0 26.5 35.0 2.0 35.0 94-95 26.86961501548864 33.0 25.5 35.0 2.0 35.0 96-97 26.257485297970348 32.0 25.0 34.0 2.0 35.0 98-99 25.377270471268016 32.0 22.0 34.0 2.0 35.0 100-101 22.97105737444855 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1318785579421231 1101 2 0.12071587290581931 1101 3 0.07823986967058971 1101 4 0.10111564822648234 1101 5 -0.04298197453012875 1101 6 0.12877492078072805 1101 7 0.03540378716819248 1101 8 0.060435552485820665 1101 9 0.2055577939781017 1101 10-11 0.09470575744627041 1101 12-13 0.1883273192942454 1101 14-15 0.16468109370971717 1101 16-17 -0.09945016146350127 1101 18-19 0.03835888652529462 1101 20-21 0.023369298940956185 1101 22-23 0.13715207505501326 1101 24-25 0.0776353025432499 1101 26-27 0.1400117223464079 1101 28-29 0.09145798469852195 1101 30-31 0.1500914564781226 1101 32-33 0.16085373979741036 1101 34-35 0.15894389934766906 1101 36-37 0.2129934269759488 1101 38-39 0.36527206033831305 1101 40-41 0.2955961776468783 1101 42-43 0.3660891599555782 1101 44-45 0.47264028535747826 1101 46-47 0.29867970228104923 1101 48-49 0.30984715774764027 1101 50-51 0.4234712991021965 1101 52-53 0.4040961225444093 1101 54-55 0.4671490450554785 1101 56-57 0.531985352923293 1101 58-59 0.45411727800221513 1101 60-61 0.6072270587762816 1101 62-63 0.5518934334850982 1101 64-65 0.5013399911003944 1101 66-67 0.7234263361960842 1101 68-69 0.5982773466166087 1101 70-71 0.5658866201415229 1101 72-73 0.4708821444941229 1101 74-75 0.5785121448556616 1101 76-77 0.48155439147872414 1101 78-79 0.6117239661273395 1101 80-81 0.6488749253356083 1101 82-83 0.5886455152969923 1101 84-85 0.6971907320596067 1101 86-87 0.7938471323316705 1101 88-89 0.795542675978588 1101 90-91 0.9747080451865173 1101 92-93 1.191910902375433 1101 94-95 1.3741452362335416 1101 96-97 1.4212745743478976 1101 98-99 1.7204169453933993 1101 100-101 2.4416275110743726 1102 1 0.10047417848638673 1102 2 0.2907047911488476 1102 3 0.19389177052185858 1102 4 0.09757153395923268 1102 5 0.006363701195361671 1102 6 0.021516256189329397 1102 7 0.0021164192333813503 1102 8 0.15923840416093782 1102 9 0.2427283278821335 1102 10-11 0.07176572408722137 1102 12-13 0.11704113007608896 1102 14-15 0.15846836948617948 1102 16-17 0.20169601533034154 1102 18-19 0.26329921115023325 1102 20-21 0.24410561001293019 1102 22-23 0.18097962744305107 1102 24-25 0.1960559360010592 1102 26-27 0.305924055864061 1102 28-29 0.1358504336302815 1102 30-31 0.35382455224770837 1102 32-33 0.3336489366408202 1102 34-35 0.39313760481868343 1102 36-37 0.3698913086992448 1102 38-39 0.41560701611368245 1102 40-41 0.3335733815398285 1102 42-43 0.7017281586380477 1102 44-45 0.7682120198231104 1102 46-47 0.8638083485090462 1102 48-49 0.8408601486615126 1102 50-51 1.0079486474903305 1102 52-53 0.9468604406522729 1102 54-55 1.2025982658054346 1102 56-57 1.1991145179686455 1102 58-59 1.196129083372881 1102 60-61 1.1945908976607313 1102 62-63 1.249599259485585 1102 64-65 1.1814321949592141 1102 66-67 1.193394092151678 1102 68-69 1.0650007873769916 1102 70-71 1.1949976220279233 1102 72-73 1.156416241945319 1102 74-75 0.9364602904248933 1102 76-77 0.8690364433559132 1102 78-79 1.074168301347072 1102 80-81 1.2210002106282758 1102 82-83 1.080859841482937 1102 84-85 1.1960654900017218 1102 86-87 1.2879285183932332 1102 88-89 1.2632218329458738 1102 90-91 1.1954147745663697 1102 92-93 1.7479647956674391 1102 94-95 1.5934923455333383 1102 96-97 1.8007587606579492 1102 98-99 1.9592453055891852 1102 100-101 2.712833033293638 1103 1 0.11773373052854197 1103 2 0.15058621407374773 1103 3 0.08559137526494709 1103 4 0.02985084884723932 1103 5 -0.12295118681517891 1103 6 0.029157889917918567 1103 7 0.008769910669485625 1103 8 0.02761785080125634 1103 9 0.10069934237488809 1103 10-11 0.047029228698555414 1103 12-13 0.06793045813932963 1103 14-15 0.22022726276681226 1103 16-17 0.04405438160175379 1103 18-19 0.1158025049578768 1103 20-21 0.03590402898526435 1103 22-23 0.13806131387256215 1103 24-25 0.13218900582977255 1103 26-27 0.14238650645695117 1103 28-29 0.1085777063575577 1103 30-31 0.16140030982347042 1103 32-33 0.19919570870932546 1103 34-35 0.13361850073436443 1103 36-37 0.06383070263863289 1103 38-39 -0.01670523685073988 1103 40-41 0.07533886243574273 1103 42-43 0.1900022034338349 1103 44-45 0.31327130836066175 1103 46-47 0.5650929334892751 1103 48-49 0.5274543515600598 1103 50-51 0.5730013484125962 1103 52-53 0.5889882403887654 1103 54-55 0.590575867913472 1103 56-57 0.6829089843718137 1103 58-59 0.48911327309620134 1103 60-61 0.4477529529966944 1103 62-63 0.5783871382734631 1103 64-65 0.6447062397550027 1103 66-67 0.6900673464336222 1103 68-69 0.6440521707762024 1103 70-71 0.6829093479831236 1103 72-73 0.7834913407595501 1103 74-75 0.7442007383827338 1103 76-77 0.699998235187266 1103 78-79 0.5943264041138718 1103 80-81 0.9515338074662196 1103 82-83 0.7538835568979572 1103 84-85 0.8165200354562678 1103 86-87 0.855484513123141 1103 88-89 1.136772557583555 1103 90-91 1.1224239972277665 1103 92-93 1.1675629511351602 1103 94-95 1.0889139529114722 1103 96-97 1.3901527000518854 1103 98-99 1.6446866758131655 1103 100-101 2.335593244097325 1104 1 0.07110257590717595 1104 2 0.16915565451908776 1104 3 0.06119603982008215 1104 4 0.10122526097892859 1104 5 -0.06686135093841727 1104 6 -0.09046011564883116 1104 7 -0.0469863999169462 1104 8 0.1118653793548603 1104 9 0.13685204416968588 1104 10-11 0.15260797905846601 1104 12-13 0.0827795319467981 1104 14-15 0.05512624539470323 1104 16-17 0.10413932257497294 1104 18-19 0.11210253375142543 1104 20-21 0.16196819061305945 1104 22-23 0.21871444757061909 1104 24-25 0.021343435237582753 1104 26-27 -0.06442916683223388 1104 28-29 0.0024626761243311535 1104 30-31 0.09673959382384822 1104 32-33 0.008743170077977425 1104 34-35 0.07382149526359427 1104 36-37 0.12919686061925262 1104 38-39 -0.16609577337056436 1104 40-41 -0.08573705655840058 1104 42-43 0.09429004295778043 1104 44-45 0.25886229597943355 1104 46-47 0.4339659059434453 1104 48-49 0.3471767337464513 1104 50-51 0.32608465775130924 1104 52-53 0.4008403855082605 1104 54-55 0.34561114001252236 1104 56-57 0.1983303299903909 1104 58-59 0.282921460062326 1104 60-61 0.5428052612796392 1104 62-63 0.531778778546041 1104 64-65 0.5219257425863617 1104 66-67 0.7182154412603836 1104 68-69 0.6147247401206677 1104 70-71 0.48234907348461675 1104 72-73 0.5624216603679564 1104 74-75 0.7183513286505097 1104 76-77 0.40227172592534544 1104 78-79 0.501282445960495 1104 80-81 0.8406810384864727 1104 82-83 0.8725837680506565 1104 84-85 0.8095387992531791 1104 86-87 0.7935039967588047 1104 88-89 0.8855104402978249 1104 90-91 0.7434372902501316 1104 92-93 0.9028146547535485 1104 94-95 1.0143182623461584 1104 96-97 1.215270273715383 1104 98-99 1.5968473918127764 1104 100-101 2.307052837148479 1105 1 0.0855049420101821 1105 2 0.18304365040413018 1105 3 0.091849289996901 1105 4 0.09664415122517767 1105 5 0.08062627202821204 1105 6 0.05268750860371796 1105 7 0.0624458639549772 1105 8 0.12183130232791939 1105 9 0.13494726695628856 1105 10-11 0.12043572933173152 1105 12-13 0.047054912902240176 1105 14-15 0.26760133058726865 1105 16-17 0.21048536513911387 1105 18-19 0.14936624493254413 1105 20-21 0.19672434698160401 1105 22-23 0.1859595128313316 1105 24-25 0.14725162015368198 1105 26-27 0.14926352911066232 1105 28-29 0.06294136190842181 1105 30-31 0.2105279304791594 1105 32-33 0.21938179285172055 1105 34-35 0.18589794353723477 1105 36-37 0.38349265564271917 1105 38-39 0.30151095844966846 1105 40-41 0.20900109685251778 1105 42-43 0.23704385251220828 1105 44-45 0.3295439972589307 1105 46-47 0.5322186546093022 1105 48-49 0.31168117018957986 1105 50-51 0.27834467189648393 1105 52-53 0.3987708490844213 1105 54-55 0.44936122038286896 1105 56-57 0.5458557270199051 1105 58-59 0.4777512356683218 1105 60-61 0.492188017018087 1105 62-63 0.5599686776008852 1105 64-65 0.43663673082014753 1105 66-67 0.4555731857469709 1105 68-69 0.2980816487556588 1105 70-71 0.6766162566647118 1105 72-73 0.7282940001094822 1105 74-75 0.9946967282518742 1105 76-77 0.8739411787640243 1105 78-79 0.966385641485477 1105 80-81 0.9461711368597783 1105 82-83 1.0257053523109292 1105 84-85 1.2077417183041632 1105 86-87 1.2118919283475655 1105 88-89 1.478880690410346 1105 90-91 1.2957583858586936 1105 92-93 1.6048536504456976 1105 94-95 1.6381770034250493 1105 96-97 1.952487370095504 1105 98-99 2.4290542007885385 1105 100-101 3.140465201322595 1106 1 -0.2975475993947292 1106 2 -0.08711625821951685 1106 3 -0.10500964775295785 1106 4 0.01895997075687461 1106 5 -0.13637829678960145 1106 6 -0.13853179465156273 1106 7 -0.12641764261270083 1106 8 -0.09064728133856192 1106 9 -0.15584370962852034 1106 10-11 -0.02103343114799827 1106 12-13 -0.2500895759612689 1106 14-15 -0.1778012964829756 1106 16-17 -0.16793410944683984 1106 18-19 0.1024073642929082 1106 20-21 -0.033522654731704904 1106 22-23 -0.37235002457585864 1106 24-25 -0.23173895665956934 1106 26-27 -0.44486839550383905 1106 28-29 -0.13371769006188572 1106 30-31 -0.22065425113135007 1106 32-33 -0.13193174227107107 1106 34-35 -0.7256525990098837 1106 36-37 -0.23947279267253663 1106 38-39 -0.05453558638185996 1106 40-41 0.05562445493361423 1106 42-43 0.10309896387714446 1106 44-45 0.07286210119953296 1106 46-47 0.4099885004116999 1106 48-49 0.08775912564360766 1106 50-51 0.1053649460255599 1106 52-53 0.3605355549667735 1106 54-55 0.24182267098252908 1106 56-57 0.12249764592338863 1106 58-59 0.302487100888186 1106 60-61 0.4022179998850177 1106 62-63 0.3505312132947722 1106 64-65 -0.07385727260646746 1106 66-67 0.04954091847113773 1106 68-69 0.18971500112573736 1106 70-71 0.368992041930273 1106 72-73 0.5497317499667069 1106 74-75 0.7045268253392543 1106 76-77 0.6434871524933214 1106 78-79 0.6567411526219722 1106 80-81 0.5841851288643447 1106 82-83 0.6166819371196084 1106 84-85 0.517500427613129 1106 86-87 0.6179099180677277 1106 88-89 0.4058795482058919 1106 90-91 0.45524582160969373 1106 92-93 0.7893553637523816 1106 94-95 0.5774902529967214 1106 96-97 1.0080342004781428 1106 98-99 1.0524765308856239 1106 100-101 1.6466759380444707 1107 1 -0.11981315098277534 1107 2 -0.08229103739636656 1107 3 -0.0707914556837963 1107 4 -0.031166885097285046 1107 5 -0.32955627085463135 1107 6 -0.05101065200920374 1107 7 -0.12739020416128 1107 8 -0.0549538026691323 1107 9 -0.011242130977890952 1107 10-11 -0.07399105189598032 1107 12-13 -0.15375195145459486 1107 14-15 -0.2401086635192442 1107 16-17 -0.3051052238990124 1107 18-19 -0.08530876049566416 1107 20-21 -0.0516278041397058 1107 22-23 -0.23679514767302123 1107 24-25 0.05492943274091999 1107 26-27 -0.05103734870812815 1107 28-29 -0.11277942657757478 1107 30-31 -0.06847420978574092 1107 32-33 -0.19531973325020857 1107 34-35 0.06541111081909179 1107 36-37 0.053963591465787886 1107 38-39 -0.21700504700956458 1107 40-41 -0.06411337630111547 1107 42-43 -0.10169571757371898 1107 44-45 -0.10775244493840574 1107 46-47 0.09006978212565997 1107 48-49 0.19997252538049537 1107 50-51 -0.09393734677654919 1107 52-53 -0.10522276113922402 1107 54-55 0.12360476081526883 1107 56-57 0.22841993496680146 1107 58-59 0.050539628608547105 1107 60-61 0.3625003716196602 1107 62-63 0.23217470136093254 1107 64-65 0.3037503199513196 1107 66-67 0.3440273871800201 1107 68-69 0.14201485077805032 1107 70-71 0.3349570860948994 1107 72-73 0.3125300961422397 1107 74-75 0.4729373684702445 1107 76-77 0.3144770979922882 1107 78-79 0.3408583298442984 1107 80-81 0.486275901275544 1107 82-83 0.2344722960746921 1107 84-85 0.33763292150525714 1107 86-87 0.7052055391915779 1107 88-89 0.9481201984596019 1107 90-91 0.9761629424516407 1107 92-93 1.0616373596178192 1107 94-95 0.7383293809801863 1107 96-97 0.9892266455072729 1107 98-99 1.2598647097993627 1107 100-101 1.7681521643177263 1108 1 -0.050813377875027044 1108 2 0.02539079834591007 1108 3 -0.01572715932572777 1108 4 -0.06603777114218445 1108 5 -0.24570425365987347 1108 6 -0.13684950945341257 1108 7 -0.04060698437627508 1108 8 -0.026322453518751843 1108 9 -0.06492974327422729 1108 10-11 -0.18301124530628954 1108 12-13 -0.03151528444043805 1108 14-15 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-1.0292021111737348 2316 70-71 -1.3176835373923659 2316 72-73 -1.1257807277516498 2316 74-75 -1.403048932236505 2316 76-77 -1.2549754143515983 2316 78-79 -1.5900280095883694 2316 80-81 -1.1054984718486658 2316 82-83 -1.4664062981745083 2316 84-85 -1.5974951160588837 2316 86-87 -1.2758066594723765 2316 88-89 -1.327275532008013 2316 90-91 -1.5037782425614274 2316 92-93 -1.685912015926764 2316 94-95 -1.7914714225825996 2316 96-97 -1.7905620562063476 2316 98-99 -1.4198719539628577 2316 100-101 -1.5309087321872603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 8.0 5 22.0 6 39.0 7 89.0 8 203.0 9 374.0 10 702.0 11 1171.0 12 1884.0 13 2688.0 14 3591.0 15 5189.0 16 7444.0 17 10629.0 18 9513.0 19 5395.0 20 4360.0 21 4302.0 22 4479.0 23 4915.0 24 5512.0 25 6467.0 26 7732.0 27 9654.0 28 11775.0 29 14654.0 30 17878.0 31 22952.0 32 29525.0 33 39035.0 34 53500.0 35 76537.0 36 115765.0 37 161900.0 38 111983.0 39 7719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63410789004745 20.050363966583824 15.484944150522933 20.83058399284579 2 15.605149626969322 21.54217609786345 39.975302433289585 22.877371841877647 3 20.017272498680207 25.705743500761596 30.468055751202293 23.8089282493559 4 12.629593108904945 17.7418314377907 37.64588481402443 31.982690639279927 5 13.021341475457104 38.79621912635433 33.71766158837246 14.464777809816104 6 30.55599804631189 37.546357306385424 16.920071249057056 14.97757339824563 7 27.63020528206701 34.58633550512185 20.178148972668115 17.605310240143023 8 26.53869395159751 34.87267456479754 19.121524929929212 19.467106553675738 9 26.67231884611283 17.41112628013307 20.624574605477434 35.29198026827666 10-11 25.174337701046223 27.466366679875563 26.333451379627665 21.025844239450546 12-13 26.62928885386776 25.161956259137913 27.381782298926392 20.82697258806793 14-15 22.999740649219515 26.20686976772965 27.1068301410368 23.686559442014037 16-17 20.93461069078146 29.80565805981919 27.64955785299682 21.61017339640253 18-19 20.9040020326782 27.80240366829957 31.05857246484612 20.235021834176116 20-21 22.125664093124104 26.085966283059843 31.371525503611497 20.41684412020456 22-23 21.46577214429966 26.273456856761445 31.32884229040556 20.93192870853333 24-25 21.720023828879683 26.48902522109185 31.48045143220124 20.31049951782723 26-27 21.386578546343806 26.521408564742437 31.781046450776262 20.31096643813749 28-29 20.672281298588793 27.318932070150865 31.67189555046783 20.33689108079251 30-31 20.945370863451185 26.66849222371938 31.50330015515041 20.882836757679023 32-33 20.88234558425675 26.98795006246799 31.34688627665751 20.78281807661775 34-35 21.571994855112436 26.567722807992215 31.301006202038206 20.55927613485714 36-37 21.557447514379486 26.539878802878924 31.386578794584967 20.516094888156623 38-39 21.268738752140962 26.935158355373762 31.439897102248715 20.35620579023656 40-41 21.137417735117282 27.000325175851675 31.252229824286555 20.610027264744488 42-43 20.954639985621938 27.259985477106035 30.67977808807352 21.105596449198508 44-45 21.413093133260226 27.161662425337912 30.386169362642168 21.039075078759698 46-47 21.720364565574275 27.660945590312657 29.696322616572914 20.922367227540157 48-49 21.84345744869923 27.8958752693891 29.363181931281257 20.897485350630408 50-51 21.567650400413907 28.10671297241008 29.440328914715717 20.885307712460293 52-53 21.848328503967277 28.43596997850153 28.67484916184937 21.04085235568182 54-55 21.361617927589787 28.17082659174896 28.97283925912566 21.494716221535594 56-57 21.894537703942525 28.25547763329906 28.80913230707659 21.04085235568182 58-59 22.114130141431747 27.793780583973703 28.847179198224303 21.244910076370246 60-61 21.656975022018486 27.911936586759417 29.62444163883363 20.806646752388463 62-63 21.483920909860462 27.86868951498771 29.618714857640114 21.02867471751171 64-65 21.30033478631207 28.186295483478563 29.29070852790127 21.22266120230809 66-67 21.384393402221463 28.140481233930455 28.864491191947224 21.61063417190086 68-69 21.349901064918 28.15910972907717 29.01338750297858 21.47760170302624 70-71 21.73366122995462 28.19175896438732 28.700586764684587 21.373993040973474 72-73 21.660346358587574 27.846064020189882 29.071332701851066 21.422256919371478 74-75 21.379075644246853 28.575142988605013 28.4560653564397 21.58971601070843 76-77 21.63525274852585 27.941623693866024 28.719544385187252 21.703579172420877 78-79 21.51821577195036 28.143245886920425 28.638382072410213 21.700156268719002 80-81 21.351480866626556 28.46776348788621 28.589671519729748 21.591084125757483 82-83 21.490531709295038 28.127814025498033 28.713592105193868 21.66806216001306 84-85 21.158824811065465 28.139709856060225 28.564874270740013 22.1365910621343 86-87 21.261103043882944 28.187743635044743 28.509496583020557 22.041656738051763 88-89 21.253862286052065 27.97131080097263 28.338153922713467 22.436672990261837 90-91 21.287543250772853 27.902475761134603 28.54965741701381 22.26032357107873 92-93 21.444449783726178 28.181028859110324 28.59501335954116 21.77950799762234 94-95 20.95705704005719 28.2028834014184 28.40035861498784 22.439700943536568 96-97 20.901829805328934 28.217628217364915 28.437483955138898 22.44305802216725 98-99 21.14441287754119 28.57721179695255 27.7657171143228 22.51265821118346 100-101 20.930093234476608 28.089897996400182 28.049673264576036 22.930335504547173 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 353.0 1 254.0 2 204.0 3 267.5 4 227.5 5 220.0 6 394.5 7 862.5 8 1425.0 9 1734.0 10 1676.5 11 1535.0 12 1598.5 13 1707.0 14 1834.0 15 1954.5 16 1999.5 17 2039.0 18 2139.5 19 2245.5 20 2438.5 21 2672.5 22 2962.5 23 3489.5 24 4114.0 25 4883.0 26 5854.5 27 7029.0 28 8042.5 29 9284.5 30 10724.5 31 11888.0 32 13162.5 33 14465.5 34 16058.5 35 17629.0 36 19622.5 37 21259.0 38 22399.0 39 22890.0 40 22829.0 41 23720.5 42 24664.5 43 25527.0 44 26210.0 45 26797.5 46 26798.0 47 28424.0 48 35069.0 49 34520.5 50 27894.5 51 25226.0 52 23359.5 53 22012.0 54 20668.0 55 19054.5 56 16944.5 57 14869.5 58 13418.0 59 11939.5 60 10178.0 61 8205.0 62 6454.5 63 5056.0 64 3707.5 65 2738.0 66 2005.5 67 1543.0 68 1094.5 69 689.0 70 529.0 71 445.5 72 378.5 73 319.5 74 254.5 75 201.5 76 153.5 77 112.5 78 72.0 79 46.5 80 37.5 81 29.0 82 14.5 83 6.5 84 4.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041601444991962756 2 0.0 3 0.0 4 0.0 5 0.010795311675129577 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0062533817630323766 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.582507118981449E-5 22-23 3.9495042713888696E-4 24-25 8.557259254675883E-4 26-27 0.002830478061162023 28-29 0.003422903701870353 30-31 4.6077549832870146E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012967539024393454 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.582507118981449E-5 74-75 6.582507118981449E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3165014237962898E-4 84-85 6.582507118981449E-5 86-87 0.0 88-89 0.0 90-91 0.008557259254675884 92-93 0.0025671777764027653 94-95 0.0 96-97 0.0 98-99 3.9495042713888696E-4 100-101 0.013954915092240672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.9535191949363 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0492463363067 49.4023679229519 2 11.420612136867751 13.92253002204982 3 3.344860433148296 6.11643043848863 4 1.376431484721182 3.3559337169786896 5 0.7359747843045705 2.243012657104887 6 0.42538409960657475 1.555719472835403 7 0.2856867989315782 1.21895310476911 8 0.20246477645603608 0.9872752510409186 9 0.16178064413056517 0.8874989637643407 >10 0.8994085584534895 10.228252215467208 >50 0.06312768209639102 2.604540378234053 >100 0.030914715382253904 3.5982065171657687 >500 0.0028104286699671683 1.2403837229325971 >1k 8.647472830668211E-4 1.2403837229325971 >5k 4.3237364153341054E-4 1.3985118932841445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5592 0.7361875961868853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5021 0.6610153648881172 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3666 0.48262942196371983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3339 0.43957982540558116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1322 0.1740414882258695 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1086 0.1429720546242771 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 978 0.12875383924727715 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 936 0.12322453326733274 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 865 0.11387737315837908 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.11005951902936983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.3165014237962898E-4 0.0 24-25 0.0 0.0 0.0 1.9747521356944345E-4 0.0 26-27 0.0 0.0 0.0 4.607754983287014E-4 0.0 28-29 0.0 0.0 0.0 0.0017772769221249913 0.0 30-31 0.0 0.0 0.0 0.004212804556148127 0.0 32-33 0.0 0.0 0.0 0.007701533329208296 0.0 34-35 0.0 0.0 0.0 0.011914337885356422 0.0 36-37 0.0 0.0 0.0 0.018101894577198983 0.0 38-39 0.0 0.0 0.0 0.02784400511329153 0.0 40-41 0.0 0.0 0.0 0.04067989399530536 0.0 42-43 0.0 0.0 0.0 0.05990081478273119 0.0 44-45 0.0 0.0 0.0 0.0803724119227635 0.0 46-47 0.0 0.0 0.0 0.11578630022288369 0.0 48-49 0.0 0.0 0.0 0.16002074806243904 0.0 50-51 0.0 0.0 0.0 0.1894445548842861 0.0 52-53 0.0 0.0 0.0 0.23236250130004515 0.0 54-55 0.0 0.0 0.0 0.2905518642318412 0.0 56-57 0.0 0.0 0.0 0.3809296869754565 0.0 58-59 0.0 0.0 0.0 0.4865131011639189 0.0 60-61 0.0 0.0 0.0 0.6051298794479647 0.0 62-63 0.0 0.0 0.0 0.7424409779499177 0.0 64-65 0.0 0.0 0.0 0.8919955396931762 0.0 66-67 0.0 0.0 0.0 1.062745774359555 0.0 68-69 0.0 0.0 0.0 1.2826015121335352 0.0 70-71 0.0 0.0 0.0 1.4989026960632659 0.0 72-73 0.0 0.0 0.0 1.7364653779873063 0.0 74-75 0.0 0.0 0.0 1.9894311265697635 0.0 76-77 0.0 0.0 0.0 2.2925555793988592 0.0 78-79 0.0 0.0 0.0 2.637610602575867 0.0 80-81 0.0 0.0 0.0 3.064288714028244 0.0 82-83 0.0 0.0 0.0 3.487280621493992 0.0 84-85 0.0 0.0 0.0 3.9671453904677403 0.0 86-87 0.0 0.0 0.0 4.439111150898709 0.0 88-89 0.0 0.0 0.0 5.060631473072938 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 185 0.0 33.378727 4 TAAGGTG 260 0.0 25.577188 5 GTGTAGC 300 0.0 25.336933 1 GTACATG 4560 0.0 23.753374 1 GTACACG 100 0.0016517644 23.753374 1 GAGTACT 4730 0.0 23.45052 12-13 ACATGGG 4560 0.0 22.708569 3 GTCTTAG 210 3.4371624E-8 22.622261 1 TACATGG 4680 0.0 22.532284 2 GTACTTT 5020 0.0 22.187515 14-15 CATGGGG 1355 0.0 21.734545 4 GGTATCA 7595 0.0 21.454662 1 AGTACTT 4880 0.0 20.734161 12-13 GGGATCG 115 0.0037204886 20.653748 7 CGTGAAC 115 0.0037219082 20.65239 3 AACCTCG 165 6.3153995E-5 20.153051 7 TAGCACT 335 3.45608E-11 19.850954 4 AGGTAAG 265 1.8519131E-8 19.717186 2 ACTTTTT 5830 0.0 19.34927 16-17 GTATCAA 9625 0.0 19.249487 1 >>END_MODULE