FastQCFastQC Report
Thu 26 May 2016
SRR937527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180931
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101190.4639761643078117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77860.3570034998814726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.24397837437314615No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27390.12558856745123986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54250.043.92261
GTATCAA89700.038.3880651
ATCAACG114500.029.643063
TCAACGC116400.029.2422184
TATCAAC117450.029.2229772
CAACGCA118450.028.7367885
AACGCAG121200.028.1424946
GTACATG88200.024.481461
TACATGG86750.024.4402482
CGCAGAG140600.024.0550088
ACGCAGA141450.023.9798267
ACATGGG88700.022.723363
GCAGAGT149650.022.1881589
GAGTACT87100.021.73356612-13
CATGGGG41600.021.1411514
GTACTTT90650.020.85504314-15
AGTACTT91000.020.82822812-13
TGGTATC29700.020.4877282
AGAGTAC136050.020.32077410-11
GAGTACA73450.019.037311
Produced by FastQC (version 0.11.5)